Rh1CG372000

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0007067
Physical Location & Seq
Forward (+)
64165909 .. 64171779
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG372000.1

Sequence Viewer

Length: 1152 bp
ATGGACCTGAAATCAAACCATACTTCTCCTGTTCTCACTGATCCTGCGCCTTTAGGCAAGTCGAGACTTGGCGTACATTCCAGTCTGTTAACATATTCAGGGTCAGGAACAGCTTTTCCAGCACTCTTAACCATCCCTAGGAGGAAGAATGGATTTCTTGATGATGTTCGCTCCAGCTCTTGGCTTGATGCTATGAAATCTTCATCGCCTCCTCACAGAAAGATAACTAAGGATGTTAACAATGACTCTCCAGCAACTGAGTATGATATTGCCTATCGCACATGGATGGTTAAGTATCCATCAGCTCTTACATCTTTTGAGCAAATCACTAATTATGCCAAGGGCAAAAGAATAGCATTGTTTCTCGATTATGATGGGACTCTTTCGCCAATTGTAGATAACCCTGACTGTGCCTTTATGTCTGATGCTATGCGAAGGGCTGTAAGAAGAGTGGCAAAACATTTCCCAACAGCAATAATTAGTGGAAGAAGCCATGACAAGGTATACGAGTTTGTTGGACTAAATGAACTATATTATGCGGGTAGTCATGGGATGGACATAATGGGCCCTGTTAGACAATCAGTAGTTGGTGACCATAGACATGGCTTTCGGTCTACTGACAGACAGGGCAAGGATGTTAATCTGTTCCAGCCTGCAGCAGAGTTTTTACCTATGATTGGCGAGGTTCTTAAATCCCTAGTAGAAACTACCAAAGACATTGAAGGAGCTAAAGTTGAGAATAACAAGTTCTGTGTCTCTGTACACTACCGCAATGTAGATGAGAAGTATTGGGCTGTAGTTGCACAGCGTGTTCATGACATTCTTAAAAACTACCCACGCCTGCGGTTGACTCATGGACGGAAGGTTTTAGAGGTCCGACCAGTGATAAATTGGGATAAAGGGAAGGCAGTCACATTTTTACTTGAATCTCTTGGGCTAAGTGATTGTGAAGATGTGCTCCCAATATATATTGGAGATGACCGCACAGATGAAGATGCATTCAAGGTTCTGAGGGAAGGAAACAGGGGTTATGGCATTTTAGTCTCTGCTGTGCCAAAGGAAAGCAATGCATTTTACTCACTAAGGGACCCCTCAGAGGTGATGGAGTTTCTGAAGTCACTGGTGATATGGAGGAAGTCAAGTGCTCTATAA

Protein Analysis

383

Amino Acids

43.06

Weight (kDa)

8.87

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 121 - 365 6.2e-71 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AccI GTMKAC 2 cut(s) 504, 614
AciI CCGC 4 cut(s) 539, 769, 844, 982
AclWI GGATC 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 1133
AdeI CACNNNGTG 1 cut(s) 809
AfaI GTAC 2 cut(s) 75, 762
AfiI CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
AgsI TTSAA 3 cut(s) 722, 926, 1003
AloI GAACNNNNNNTCC 2 cut(s) 100, 132
AluBI AGCT 4 cut(s) 113, 177, 305, 728
AluI AGCT 4 cut(s) 113, 177, 305, 728
Alw21I GWGCWC 2 cut(s) 960, 1147
Alw26I GTCTC 3 cut(s) 58, 760, 1048
AlwI GGATC 1 cut(s) 35
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 1 cut(s) 565
ApaI GGGCCC 1 cut(s) 569
ApeKI GCWGC 1 cut(s) 656
ArsI GACNNNNNNTTYG 2 cut(s) 591, 623
AspA2I CCTAGG 1 cut(s) 137
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
AsuHPI GGTGA 3 cut(s) 602, 1111, 1135
AvaII GGWCC 3 cut(s) 4, 874, 1087
AvrII CCTAGG 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 569
BanII GRGCYC 1 cut(s) 569
Bbv12I GWGCWC 2 cut(s) 960, 1147
BbvI GCAGC 1 cut(s) 668
BccI CCATC 6 cut(s) 140, 280, 307, 368, 547, 1096
BciVI GTATCC 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 58, 760, 1048
BfaI CTAG 2 cut(s) 138, 698
BfmI CTRYAG 2 cut(s) 654, 795
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 1 cut(s) 657
BlnI CCTAGG 1 cut(s) 137
BlsI GCNGC 1 cut(s) 658
Bme18I GGWCC 3 cut(s) 4, 874, 1087
BmgT120I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
BmiI GGNNCC 3 cut(s) 567, 1088, 1089
BmsI GCATC 3 cut(s) 178, 415, 985
BpmI CTGGAG 2 cut(s) 157, 234
BsaBI GATNNNNATC 1 cut(s) 639
BsaJI CCNNGG 2 cut(s) 137, 339
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
Bse1I ACTGG 3 cut(s) 81, 881, 1125
Bse3DI GCAATG 2 cut(s) 778, 1072
Bse8I GATNNNNATC 1 cut(s) 639
BseDI CCNNGG 2 cut(s) 137, 339
BseGI GGATG 5 cut(s) 132, 238, 291, 558, 640
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
BseMI GCAATG 2 cut(s) 778, 1072
BseMII CTCAG 3 cut(s) 249, 1001, 1107
BseNI ACTGG 3 cut(s) 81, 881, 1125
BseRI GAGGAG 1 cut(s) 201
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 1 cut(s) 668
BshFI GGCC 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 960, 1147
BslFI GGGAC 2 cut(s) 391, 1100
BslI CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
BsmAI GTCTC 3 cut(s) 58, 760, 1048
BsmFI GGGAC 2 cut(s) 391, 1100
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 1 cut(s) 567
Bsp120I GGGCCC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 569, 960, 1147
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 4 cut(s) 539, 769, 844, 982
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 3 cut(s) 250, 1002, 1106
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 3 cut(s) 567, 1088, 1089
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 778, 1072
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 3 cut(s) 81, 881, 1125
BssECI CCNNGG 2 cut(s) 137, 339
BssMI GATC 1 cut(s) 40
BssNAI GTATAC 1 cut(s) 505
BssT1I CCWWGG 2 cut(s) 137, 339
Bst1107I GTATAC 1 cut(s) 505
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 442
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 2 cut(s) 654, 842
BstDEI CTNAG 6 cut(s) 228, 258, 938, 1010, 1082, 1093
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 5 cut(s) 132, 238, 291, 558, 640
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 3 cut(s) 58, 760, 1048
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 119, 800
BstPI GGTNACC 1 cut(s) 590
BstSFI CTRYAG 2 cut(s) 654, 795
BstSLI GKGCMC 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 668
BstXI CCANNNNNNTGG 1 cut(s) 602
BstZ17I GTATAC 1 cut(s) 505
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 1 cut(s) 567
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 5 cut(s) 132, 238, 291, 558, 640
BtsIMutI CAGTG 3 cut(s) 36, 888, 1118
Cac8I GCNNGC 2 cut(s) 654, 842
CaiI CAGNNNCTG 1 cut(s) 257
CciI TCATGA 1 cut(s) 814
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
Csp6I GTAC 2 cut(s) 74, 761
CviAII CATG 6 cut(s) 282, 494, 548, 602, 815, 854
CviQI GTAC 2 cut(s) 74, 761
DdeI CTNAG 6 cut(s) 228, 258, 938, 1010, 1082, 1093
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DraIII CACNNNGTG 1 cut(s) 809
Eam1104I CTCTTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 137, 339
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 3 cut(s) 4, 874, 1087
Eco57I CTGAAG 1 cut(s) 1133
Eco91I GGTNACC 1 cut(s) 590
EcoO109I RGGNCCY 2 cut(s) 566, 1087
EcoO65I GGTNACC 1 cut(s) 590
EcoT14I CCWWGG 2 cut(s) 137, 339
EcoT22I ATGCAT 2 cut(s) 1000, 1072
EcoT38I GRGCYC 1 cut(s) 569
ErhI CCWWGG 2 cut(s) 137, 339
FaeI CATG 6 cut(s) 285, 497, 551, 605, 818, 857
FaqI GGGAC 2 cut(s) 391, 1100
FatI CATG 6 cut(s) 281, 493, 547, 601, 814, 853
FauI CCCGC 1 cut(s) 532
FblI GTMKAC 2 cut(s) 504, 614
Fnu4HI GCNGC 1 cut(s) 657
FokI GGATG 5 cut(s) 119, 245, 298, 565, 647
FriOI GRGCYC 1 cut(s) 569
Fsp4HI GCNGC 1 cut(s) 657
FspBI CTAG 2 cut(s) 138, 698
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 657
GsuI CTGGAG 2 cut(s) 157, 234
HaeIII GGCC 1 cut(s) 567
HhaI GCGC 1 cut(s) 49
Hin1II CATG 6 cut(s) 285, 497, 551, 605, 818, 857
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 3 cut(s) 90, 238, 849
HindII GTYRAC 3 cut(s) 90, 238, 849
HinfI GANTC 4 cut(s) 245, 379, 850, 926
HpaI GTTAAC 2 cut(s) 90, 238
HphI GGTGA 3 cut(s) 602, 1111, 1135
Hpy166II GTNNAC 6 cut(s) 90, 238, 505, 615, 763, 849
Hpy188I TCNGA 5 cut(s) 424, 878, 1011, 1096, 1113
Hpy188III TCNNGA 5 cut(s) 63, 105, 158, 365, 815
Hpy8I GTNNAC 6 cut(s) 90, 238, 505, 615, 763, 849
HpyAV CCTTC 5 cut(s) 429, 716, 856, 898, 1010
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 4 cut(s) 656, 803, 998, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 800
HpyF3I CTNAG 6 cut(s) 228, 258, 938, 1010, 1082, 1093
Hsp92II CATG 6 cut(s) 285, 497, 551, 605, 818, 857
HspAI GCGC 1 cut(s) 47
KflI GGGWCCC 1 cut(s) 1087
KspAI GTTAAC 2 cut(s) 90, 238
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 176, 725, 963
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 3 cut(s) 178, 415, 985
MaeI CTAG 2 cut(s) 138, 698
MaeIII GTNAC 3 cut(s) 590, 910, 1116
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 6 cut(s) 157, 192, 459, 498, 962, 1004
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 3 cut(s) 569, 960, 1147
MluCI AATT 4 cut(s) 331, 390, 477, 889
MlyI GAGTC 3 cut(s) 239, 373, 844
MmeI TCCRAC 2 cut(s) 496, 901
MnlI CCTC 9 cut(s) 135, 219, 222, 676, 865, 1005, 1090, 1102, 1125
Mph1103I ATGCAT 2 cut(s) 1000, 1072
MseI TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
MslI CAYNNNNRTG 2 cut(s) 284, 600
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 1 cut(s) 998
MwoI GCNNNNNNNGC 2 cut(s) 119, 800
NdeII GATC 1 cut(s) 40
NlaIII CATG 6 cut(s) 285, 497, 551, 605, 818, 857
NlaIV GGNNCC 3 cut(s) 567, 1088, 1089
NmuCI GTSAC 3 cut(s) 590, 910, 1116
NsiI ATGCAT 2 cut(s) 1000, 1072
PagI TCATGA 1 cut(s) 814
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 1 cut(s) 926
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 1 cut(s) 658
PleI GAGTC 3 cut(s) 239, 373, 844
PpsI GAGTC 3 cut(s) 239, 373, 844
PpuMI RGGWCCY 1 cut(s) 1087
Psp5II RGGWCCY 1 cut(s) 1087
PspEI GGTNACC 1 cut(s) 590
PspN4I GGNNCC 3 cut(s) 567, 1088, 1089
PspOMI GGGCCC 1 cut(s) 565
PspPI GGNCC 5 cut(s) 4, 565, 566, 874, 1087
PspPPI RGGWCCY 1 cut(s) 1087
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 75, 762
RsaNI GTAC 2 cut(s) 74, 761
RseI CAYNNNNRTG 2 cut(s) 284, 600
SaqAI TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
SatI GCNGC 1 cut(s) 657
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
SchI GAGTC 3 cut(s) 239, 373, 844
SduI GDGCHC 3 cut(s) 569, 960, 1147
SfaNI GCATC 3 cut(s) 178, 415, 985
SfcI CTRYAG 2 cut(s) 654, 795
SinI GGWCC 3 cut(s) 4, 874, 1087
SmiMI CAYNNNNRTG 2 cut(s) 284, 600
Sse9I AATT 4 cut(s) 331, 390, 477, 889
SsiI CCGC 4 cut(s) 539, 769, 844, 982
SspMI CTAG 2 cut(s) 138, 698
StyI CCWWGG 2 cut(s) 137, 339
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 2 cut(s) 62, 366
TaqII GACCGA 1 cut(s) 600
TasI AATT 4 cut(s) 331, 390, 477, 889
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 1 cut(s) 926
Tru1I TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
Tru9I TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
TscAI CASTG 3 cut(s) 43, 888, 1125
TseFI GTSAC 3 cut(s) 590, 910, 1116
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 3 cut(s) 590, 910, 1116
TspDTI ATGAA 5 cut(s) 192, 209, 540, 803, 1005
TspGWI ACGGA 1 cut(s) 874
TspRI CASTG 3 cut(s) 43, 888, 1125
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 3 cut(s) 4, 874, 1087
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XmaJI CCTAGG 1 cut(s) 137
XmiI GTMKAC 2 cut(s) 504, 614
XspI CTAG 2 cut(s) 138, 698
Zsp2I ATGCAT 2 cut(s) 1000, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.