MD11G1196000.v1.1

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007067
Physical Location & Seq
Reverse (-)
28062018 .. 28066719
4702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1196000.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGACCTGAAATCAAATCATGCTTCTCCTGTTCTTGCTGACCCTGCGCCCTTGAGCAAGTCAAGACTTGGTGGAGTCCATACCAGCCTGTTACCATATGCACCTCCAGGAGCAGCTTTCCCGGCAGGTTTATTCCTAACAATCCCTAGGTGGAAGACTGGATTACTTGATGATGTTCGCTCTAGCTCTTGGCTCGATGCCATGAAATCTTCATCGCCTCCTCACAGAAAGATAACGAAGGGTGTTAAAAATGAGTCCGGGGCGACAGATGCTGATATTGCTTACCACTCATGGATGGTCAAGTATCCATCAGCGCTTACATCTTTTGAGCAAATCTCTAATCATGCCAAAGGCAAGAGAATAGCACTGTTTCTTGATTATGACGGGACTCTTTCACCGATTGTGGATAACCCTGACTGTGCCTTTATGTCTGATGCCCCGTTAAAAAGGGTAGCAAAACATTTCCCAACAGCAATTATTAGTGGAAGAAGCCATGACAAGGTATACGAATTTGTAAAACTAAATGAACTCTATTACGCCGGTAGTCATGGAATGGACATAATGGCCCCTGTTATACAATCTATAAACGATGACCAGAGAAACTGTTTTAAGTCAACTGACAAAAAGGGTAAGGCGGTTAACTTGTTCCAGCCTGCAGCTGAATTTTTACCTATGATTAGAGAGGTATTTAAATTGCTTGTTGAAAGTACGAAAGACATCCAAGGAGCTAAAGTTGAGGACAATAAGTTCTGTGTCTCTGTACATTACCGCAACGTAGATGAGAAGAGTTGGCCTGCAGTTGCGCAATGTGTCCATGAAATTCTTAAAGACTACCCACGTTTGCGCTTGACTCACGGGCGAAAGGTTTTGGAGGTTCGACCTACAATAAACTGGGATAAAGGGAAGGCCGTAATGTTTTTACTTGAATCTCTCGGGCTAAGTAATTGCGAAGATGTGCTCCCGATATATATTGGTGACGATCGGACAGATGAGGATGCATTCAAGGTCTTGAGGGAAGGAAACCGAGGCTATGGCATTCTGGTCTCGTCCGTGCCCAAGGACAGCAATGCATTCTACTCTCTAAGGGAACCCTTGGAGGTAATGGAGTTTCTCAAGTCACTGGTTTTATGGAGGAAGTCAAGTGCTCTATAA

Protein Analysis

384

Amino Acids

42.76

Weight (kDa)

8.6

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 124 - 364 4.6e-69 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 804
Acc36I ACCTGC 1 cut(s) 116
AccI GTMKAC 1 cut(s) 504
AciI CCGC 2 cut(s) 635, 769
AcsI RAATTY 3 cut(s) 509, 662, 819
AfaI GTAC 2 cut(s) 709, 762
AfeI AGCGCT 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 3 cut(s) 704, 926, 1003
AjnI CCWGG 1 cut(s) 106
AloI GAACNNNNNNTCC 2 cut(s) 731, 763
AluBI AGCT 4 cut(s) 116, 186, 659, 728
AluI AGCT 4 cut(s) 116, 186, 659, 728
Alw21I GWGCWC 2 cut(s) 960, 1147
Alw26I GTCTC 2 cut(s) 760, 1048
AlwNI CAGNNNCTG 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 932
Aor51HI AGCGCT 1 cut(s) 315
AoxI GGCC 3 cut(s) 564, 791, 906
ApeKI GCWGC 2 cut(s) 113, 656
ApoI RAATTY 3 cut(s) 509, 662, 819
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 4 cut(s) 49, 316, 805, 846
AspS9I GGNCC 2 cut(s) 4, 565
AsuC2I CCSGG 2 cut(s) 122, 259
AsuHPI GGTGA 2 cut(s) 387, 986
AvaI CYCGRG 1 cut(s) 932
AvaII GGWCC 1 cut(s) 4
AvrII CCTAGG 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 1056
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 960, 1147
BbvI GCAGC 2 cut(s) 125, 668
BccI CCATC 2 cut(s) 289, 316
BceAI ACGGC 1 cut(s) 893
BciT130I CCWGG 1 cut(s) 108
BciVI GTATCC 1 cut(s) 315
BcnI CCSGG 2 cut(s) 122, 259
BcoDI GTCTC 2 cut(s) 760, 1048
BfaI CTAG 2 cut(s) 147, 183
BfmI CTRYAG 2 cut(s) 654, 795
BfoI RGCGCY 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 116
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 2 cut(s) 114, 657
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 2 cut(s) 115, 658
Bme1390I CCNGG 3 cut(s) 108, 122, 259
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 932
BmgT120I GGNCC 2 cut(s) 4, 565
BmiI GGNNCC 2 cut(s) 567, 1089
BmrFI CCNGG 3 cut(s) 108, 122, 259
BmrI ACTGGG 1 cut(s) 901
BmsI GCATC 4 cut(s) 187, 259, 424, 985
BmuI ACTGGG 1 cut(s) 901
BpiI GAAGAC 1 cut(s) 161
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 1 cut(s) 90
BpuEI CTTGAG 3 cut(s) 73, 1030, 1097
BpuMI CCSGG 2 cut(s) 122, 259
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 6 cut(s) 146, 258, 721, 1024, 1056, 1092
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 3 cut(s) 163, 896, 1125
Bse3DI GCAATG 2 cut(s) 812, 1072
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 6 cut(s) 146, 258, 721, 1024, 1056, 1092
BseGI GGATG 3 cut(s) 300, 717, 1000
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMI GCAATG 2 cut(s) 812, 1072
BseNI ACTGG 3 cut(s) 163, 896, 1125
BseRI GAGGAG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 1056
BseXI GCAGC 2 cut(s) 125, 668
Bsh1285I CGRYCG 1 cut(s) 982
BshFI GGCC 3 cut(s) 566, 793, 908
BsiEI CGRYCG 1 cut(s) 982
BsiHKAI GWGCWC 2 cut(s) 960, 1147
BsiHKCI CYCGRG 1 cut(s) 932
BsiSI CCGG 3 cut(s) 122, 258, 540
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 2 cut(s) 760, 1048
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 3 cut(s) 998, 1035, 1070
BsnI GGCC 3 cut(s) 566, 793, 908
Bso31I GGTCTC 1 cut(s) 1048
BsoBI CYCGRG 1 cut(s) 932
Bsp1286I GDGCHC 3 cut(s) 960, 1056, 1147
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 1 cut(s) 979
BspACI CCGC 2 cut(s) 635, 769
BspANI GGCC 3 cut(s) 566, 793, 908
BspLI GGNNCC 2 cut(s) 567, 1089
BspMAI CTGCAG 2 cut(s) 658, 799
BspMI ACCTGC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 1048
BsrDI GCAATG 2 cut(s) 812, 1072
BsrFI RCCGGY 1 cut(s) 539
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 3 cut(s) 163, 896, 1125
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 6 cut(s) 146, 258, 721, 1024, 1056, 1092
BssMI GATC 1 cut(s) 979
BssNAI GTATAC 1 cut(s) 505
BssT1I CCWWGG 4 cut(s) 146, 721, 1056, 1092
Bst1107I GTATAC 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 369, 419, 605
Bst6I CTCTTC 1 cut(s) 779
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 2 cut(s) 654, 795
BstDEI CTNAG 2 cut(s) 938, 1082
BstF5I GGATG 3 cut(s) 300, 717, 1000
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 4 cut(s) 49, 316, 805, 846
BstKTI GATC 1 cut(s) 982
BstMAI GTCTC 2 cut(s) 760, 1048
BstMBI GATC 1 cut(s) 979
BstMCI CGRYCG 1 cut(s) 982
BstMWI GCNNNNNNNGC 4 cut(s) 44, 122, 269, 278
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 3 cut(s) 106, 120, 257
BstSFI CTRYAG 2 cut(s) 654, 795
BstSLI GKGCMC 1 cut(s) 1056
BstV1I GCAGC 2 cut(s) 125, 668
BstV2I GAAGAC 1 cut(s) 161
BstZ17I GTATAC 1 cut(s) 505
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 3 cut(s) 566, 793, 908
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 3 cut(s) 300, 717, 1000
BtsIMutI CAGTG 2 cut(s) 365, 1118
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 2 cut(s) 654, 795
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 4 cut(s) 49, 316, 805, 846
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 2 cut(s) 4, 565
Csp6I GTAC 2 cut(s) 708, 761
CviAII CATG 7 cut(s) 20, 202, 291, 344, 494, 548, 815
CviQI GTAC 2 cut(s) 708, 761
DdeI CTNAG 2 cut(s) 938, 1082
DpnI GATC 1 cut(s) 981
DpnII GATC 1 cut(s) 979
DraI TTTAAA 1 cut(s) 691
Eam1104I CTCTTC 1 cut(s) 779
EarI CTCTTC 1 cut(s) 779
Eco130I CCWWGG 4 cut(s) 146, 721, 1056, 1092
Eco31I GGTCTC 1 cut(s) 1048
Eco47I GGWCC 1 cut(s) 4
Eco47III AGCGCT 1 cut(s) 315
Eco88I CYCGRG 1 cut(s) 932
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 4 cut(s) 146, 721, 1056, 1092
EcoT22I ATGCAT 2 cut(s) 1000, 1072
ErhI CCWWGG 4 cut(s) 146, 721, 1056, 1092
FaeI CATG 7 cut(s) 23, 205, 294, 347, 497, 551, 818
FaqI GGGAC 1 cut(s) 400
FatI CATG 7 cut(s) 19, 201, 290, 343, 493, 547, 814
FauNDI CATATG 1 cut(s) 97
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 2 cut(s) 114, 657
FokI GGATG 3 cut(s) 307, 704, 1007
Fsp4HI GCNGC 2 cut(s) 114, 657
FspBI CTAG 2 cut(s) 147, 183
FspI TGCGCA 1 cut(s) 804
GlaI GCGC 4 cut(s) 48, 315, 804, 845
GluI GCNGC 2 cut(s) 114, 657
GsuI CTGGAG 1 cut(s) 90
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 3 cut(s) 566, 793, 908
HapII CCGG 3 cut(s) 122, 258, 540
HhaI GCGC 4 cut(s) 49, 316, 805, 846
Hin1II CATG 7 cut(s) 23, 205, 294, 347, 497, 551, 818
Hin6I GCGC 4 cut(s) 47, 314, 803, 844
HinP1I GCGC 4 cut(s) 47, 314, 803, 844
HincII GTYRAC 2 cut(s) 615, 640
HindII GTYRAC 2 cut(s) 615, 640
HinfI GANTC 5 cut(s) 75, 254, 388, 850, 926
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 3 cut(s) 122, 258, 540
HphI GGTGA 2 cut(s) 387, 986
Hpy166II GTNNAC 3 cut(s) 505, 615, 640
Hpy188I TCNGA 2 cut(s) 433, 984
Hpy188III TCNNGA 4 cut(s) 63, 374, 961, 1009
Hpy8I GTNNAC 3 cut(s) 505, 615, 640
HpyAV CCTTC 3 cut(s) 232, 898, 1010
HpyCH4III ACNGT 3 cut(s) 369, 419, 605
HpyCH4IV ACGT 2 cut(s) 774, 838
HpyCH4V TGCA 5 cut(s) 101, 656, 797, 998, 1070
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 122, 269, 278
HpyF3I CTNAG 2 cut(s) 938, 1082
HpySE526I ACGT 2 cut(s) 774, 838
Hsp92II CATG 7 cut(s) 23, 205, 294, 347, 497, 551, 818
HspAI GCGC 4 cut(s) 47, 314, 803, 844
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 1 cut(s) 979
LmnI GCTCC 3 cut(s) 110, 725, 963
Lsp1109I GCAGC 2 cut(s) 125, 668
LweI GCATC 4 cut(s) 187, 259, 424, 985
MaeI CTAG 2 cut(s) 147, 183
MaeII ACGT 2 cut(s) 774, 838
MaeIII GTNAC 3 cut(s) 90, 974, 1116
MalI GATC 1 cut(s) 981
MboI GATC 1 cut(s) 979
MboII GAAGA 5 cut(s) 166, 201, 498, 796, 962
MhlI GDGCHC 3 cut(s) 960, 1056, 1147
MluCI AATT 6 cut(s) 474, 509, 662, 692, 819, 943
MlyI GAGTC 4 cut(s) 84, 263, 382, 844
Mph1103I ATGCAT 2 cut(s) 1000, 1072
MseI TTAA 6 cut(s) 246, 443, 609, 639, 690, 825
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 3 cut(s) 122, 258, 540
MspR9I CCNGG 3 cut(s) 108, 122, 259
Mva1269I GAATGC 3 cut(s) 998, 1035, 1070
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 4 cut(s) 44, 122, 269, 278
NciI CCSGG 2 cut(s) 122, 259
NdeI CATATG 1 cut(s) 97
NdeII GATC 1 cut(s) 979
NlaIII CATG 7 cut(s) 23, 205, 294, 347, 497, 551, 818
NlaIV GGNNCC 2 cut(s) 567, 1089
NmuCI GTSAC 2 cut(s) 974, 1116
NsbI TGCGCA 1 cut(s) 804
NsiI ATGCAT 2 cut(s) 1000, 1072
PctI GAATGC 3 cut(s) 998, 1035, 1070
PfeI GAWTC 1 cut(s) 926
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 2 cut(s) 115, 658
Ple19I CGATCG 1 cut(s) 982
PleI GAGTC 4 cut(s) 83, 262, 382, 844
PpsI GAGTC 4 cut(s) 83, 262, 382, 844
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 567, 1089
PspPI GGNCC 2 cut(s) 4, 565
PstI CTGCAG 2 cut(s) 658, 799
PstNI CAGNNNCTG 1 cut(s) 272
PvuI CGATCG 1 cut(s) 982
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 2 cut(s) 709, 762
RsaNI GTAC 2 cut(s) 708, 761
SaqAI TTAA 6 cut(s) 246, 443, 609, 639, 690, 825
SatI GCNGC 2 cut(s) 114, 657
Sau3AI GATC 1 cut(s) 979
Sau96I GGNCC 2 cut(s) 4, 565
SchI GAGTC 4 cut(s) 84, 263, 382, 844
ScrFI CCNGG 3 cut(s) 108, 122, 259
SduI GDGCHC 3 cut(s) 960, 1056, 1147
SfaNI GCATC 4 cut(s) 187, 259, 424, 985
SfcI CTRYAG 2 cut(s) 654, 795
SinI GGWCC 1 cut(s) 4
SmiI ATTTAAAT 1 cut(s) 691
SmlI CTYRAG 3 cut(s) 52, 1009, 1112
SmoI CTYRAG 3 cut(s) 52, 1009, 1112
Sse9I AATT 6 cut(s) 474, 509, 662, 692, 819, 943
SsiI CCGC 2 cut(s) 635, 769
SspMI CTAG 2 cut(s) 147, 183
StyD4I CCNGG 3 cut(s) 106, 120, 257
StyI CCWWGG 4 cut(s) 146, 721, 1056, 1092
SwaI ATTTAAAT 1 cut(s) 691
TaaI ACNGT 3 cut(s) 369, 419, 605
TaiI ACGT 2 cut(s) 777, 841
TaqI TCGA 2 cut(s) 195, 877
TasI AATT 6 cut(s) 474, 509, 662, 692, 819, 943
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 1 cut(s) 926
Tru1I TTAA 6 cut(s) 246, 443, 609, 639, 690, 825
Tru9I TTAA 6 cut(s) 246, 443, 609, 639, 690, 825
TscAI CASTG 2 cut(s) 372, 1125
TseFI GTSAC 2 cut(s) 974, 1116
TseI GCWGC 2 cut(s) 113, 656
Tsp45I GTSAC 2 cut(s) 974, 1116
TspDTI ATGAA 4 cut(s) 201, 218, 540, 831
TspGWI ACGGA 1 cut(s) 1039
TspRI CASTG 2 cut(s) 372, 1125
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 3 cut(s) 509, 662, 819
XmaJI CCTAGG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 2 cut(s) 147, 183
Zsp2I ATGCAT 2 cut(s) 1000, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.