Rorug01G0383600

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007067
Physical Location & Seq
Forward (+)
49566461 .. 49569037
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0383600.1

Sequence Viewer

Length: 1329 bp
ATGAACACCATTGAAGTCGATTCTGGGCCCAATACCAAAGTTTTCGATGAAGAGACATTGGGCCTGGACGTCCCAGAAACCGCCCATCAGATTAGCACAGATTCATGGTTTCAAGCGGGGTTTGTGCTGACAACTGGAATCAACAGTGCATATGTGCTGGGATATTCCGGAACTGTGATGGTTCCTCTGAGTTGGATAGGCGGTGTAGTCGGTTTGCTTTTAGCCACTGCAATATCACTCTATGCGAATACTCTGATTGCCAAGCTTCATTTATTTGGAGGCAAGAGGCACATCAGATACAGGGATCTTGCAGGGTTTATATACGGAAAGAAAGCTTATGCTCTTACATGGGGATTGCAATATGTTAATCTTTTCATGATCAACACTGGATTTATCATTTTGGCTGGCCAGGCCCTCAAGGCTGCCTATGTTCTGTTCAGGGATGATGATCTTATGAAGCTACCTTACTTTATTGCTATTGGTGGTTTTGTTTGTGCATTATTTGCCATTGGGATACCACACTTGTCAGCTCTAAGGGTTTGGCTGGGGGTTTCAACAGTCTTGAGCTTGGTCTATATTATTGCAGCACTGGTCCTGTCGCTTAAAGATGGAATCAAAGCACCACCCAGGGATTATAGTATCCCAGGGACACATATAAGCAAAATATTTACAACAATTGGAGCAACTGCTAACCTTGTTTTCGCATTCAATACCGGAATGCTTCCAGAGATACAGGCTACAGTGAGGAAGCCAGTGGTTGAAAACATGCTAAAAGCTCTGTACTTTCAGTTCACAGCTGGTGTTTTGCCAATGTATGCAGTAACTTTTGTAGGGTATTGGGCATATGGAAACTCAACATCAGCTTACTTGCTCAACAATGTAAATGGACCGGTTTGGGTGAAGGCATTTGCCAATATCTGTGCCTTCCTGCAATCAGTCATTGCTTTGCATATATTTGCAAGTCCAATGTATGAGTATCTGGACACAAGGTTTGGGATTAAAGGAAGTGCATTCAAAATCCAGAACTTGACATTCAGAATTACGGTTAGAGGCGGCTATCTAGCTGTAAACACATTGGTTTCAGCTCTTTTGCCATTCCTTGGAGATTTCATGAGTCTCACAGGAGCCATCAGCACATTCCCTCTTACATTCATTCTCGCAAACCACATGTACCTGGTGGCGAAGAAGGACAAGCTCACTTCATCACAAAAGCTCTGGCATTGGCTAAATATTTGCGTCTTTGGCTTTTTGGCAGTTGCAGCCTTAATATCAGCCTTGAGGCTCATTGCAGTAGATTCCAAAACTTACCATGTCTTTGCTGATCTATGA

Protein Analysis

442

Amino Acids

48.41

Weight (kDa)

9.24

Isoelectric Point (pI)

25.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 33 - 426 5.1e-85 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 4 cut(s) 81, 116, 201, 1053
AclWI GGATC 1 cut(s) 312
AcoI YGGCCR 1 cut(s) 406
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 2 cut(s) 782, 1172
AfiI CCNNNNNNNGG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 1167
AgeI ACCGGT 1 cut(s) 889
AgsI TTSAA 6 cut(s) 14, 113, 555, 709, 761, 1015
AjnI CCWGG 5 cut(s) 63, 408, 626, 643, 1173
Alw26I GTCTC 2 cut(s) 47, 1121
AlwI GGATC 1 cut(s) 312
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 4 cut(s) 26, 61, 406, 411
ApaI GGGCCC 1 cut(s) 30
ApeKI GCWGC 3 cut(s) 422, 584, 1259
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AsiGI ACCGGT 1 cut(s) 889
AspS9I GGNCC 6 cut(s) 26, 27, 61, 412, 592, 887
AsuHPI GGTGA 1 cut(s) 910
AvaII GGWCC 2 cut(s) 592, 887
BaeGI GKGCMC 1 cut(s) 30
BalI TGGCCA 1 cut(s) 408
BanII GRGCYC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 449, 481
BbvI GCAGC 3 cut(s) 409, 596, 1271
BccI CCATC 4 cut(s) 93, 172, 602, 1136
BciT130I CCWGG 5 cut(s) 65, 410, 628, 645, 1175
BciVI GTATCC 2 cut(s) 507, 650
BclI TGATCA 1 cut(s) 378
BcoDI GTCTC 2 cut(s) 47, 1121
BfaI CTAG 1 cut(s) 1061
BfmI CTRYAG 1 cut(s) 738
BfuI GTATCC 2 cut(s) 507, 650
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 4 cut(s) 423, 585, 1054, 1260
BlsI GCNGC 4 cut(s) 424, 586, 1055, 1261
Bme1390I CCNGG 5 cut(s) 65, 410, 628, 645, 1175
Bme18I GGWCC 2 cut(s) 592, 887
BmgT120I GGNCC 6 cut(s) 26, 27, 61, 412, 592, 887
BmiI GGNNCC 3 cut(s) 28, 183, 1126
BmrFI CCNGG 5 cut(s) 65, 410, 628, 645, 1175
BpuEI CTTGAG 3 cut(s) 401, 583, 1297
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 5 cut(s) 626, 627, 643, 644, 1099
BsaWI WCCGGW 3 cut(s) 167, 713, 889
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 4 cut(s) 139, 391, 594, 752
Bse3DI GCAATG 2 cut(s) 939, 1284
Bse8I GATNNNNATC 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 5 cut(s) 65, 410, 628, 645, 1175
BseDI CCNNGG 5 cut(s) 626, 627, 643, 644, 1099
BseGI GGATG 1 cut(s) 448
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMI GCAATG 2 cut(s) 939, 1284
BseMII CTCAG 1 cut(s) 179
BseNI ACTGG 4 cut(s) 139, 391, 594, 752
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 3 cut(s) 409, 596, 1271
BseYI CCCAGC 2 cut(s) 157, 544
BshFI GGCC 4 cut(s) 28, 63, 408, 413
BshTI ACCGGT 1 cut(s) 889
BsiSI CCGG 3 cut(s) 168, 714, 890
BslFI GGGAC 2 cut(s) 56, 661
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 2 cut(s) 47, 1121
BsmFI GGGAC 2 cut(s) 56, 661
BsmI GAATGC 3 cut(s) 704, 723, 1010
BsnI GGCC 4 cut(s) 28, 63, 408, 413
Bsp120I GGGCCC 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 30
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 4 cut(s) 304, 378, 448, 1321
BspACI CCGC 4 cut(s) 81, 116, 201, 1053
BspANI GGCC 4 cut(s) 28, 63, 408, 413
BspCNI CTCAG 1 cut(s) 180
BspEI TCCGGA 1 cut(s) 167
BspHI TCATGA 2 cut(s) 375, 1110
BspLI GGNNCC 3 cut(s) 28, 183, 1126
BspPI GGATC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 939, 1284
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 4 cut(s) 139, 391, 594, 752
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 5 cut(s) 626, 627, 643, 644, 1099
BssMI GATC 4 cut(s) 304, 378, 448, 1321
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 1099
Bst2UI CCWGG 5 cut(s) 65, 410, 628, 645, 1175
Bst4CI ACNGT 5 cut(s) 146, 175, 559, 742, 1045
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 2 cut(s) 188, 533
BstF5I GGATG 1 cut(s) 448
BstKTI GATC 4 cut(s) 307, 381, 451, 1324
BstMAI GTCTC 2 cut(s) 47, 1121
BstMBI GATC 4 cut(s) 304, 378, 448, 1321
BstMWI GCNNNNNNNGC 5 cut(s) 410, 419, 503, 1242, 1259
BstNI CCWGG 5 cut(s) 65, 410, 628, 645, 1175
BstNSI RCATGY 2 cut(s) 769, 1171
BstSCI CCNGG 5 cut(s) 63, 408, 626, 643, 1173
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 409, 596, 1271
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuI GTATCC 2 cut(s) 507, 650
BsuRI GGCC 4 cut(s) 28, 63, 408, 413
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 6 cut(s) 151, 225, 384, 587, 747, 759
Cac8I GCNNGC 1 cut(s) 406
CciI TCATGA 2 cut(s) 375, 1110
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 6 cut(s) 26, 27, 61, 412, 592, 887
CseI GACGC 1 cut(s) 1225
CsiI ACCWGGT 1 cut(s) 1173
Csp6I GTAC 2 cut(s) 781, 1171
CspAI ACCGGT 1 cut(s) 889
CviAII CATG 7 cut(s) 105, 348, 376, 766, 1111, 1168, 1310
CviQI GTAC 2 cut(s) 781, 1171
DdeI CTNAG 2 cut(s) 188, 533
DpnI GATC 4 cut(s) 306, 380, 450, 1323
DpnII GATC 4 cut(s) 304, 378, 448, 1321
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 1099
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 2 cut(s) 592, 887
EcoO109I RGGNCCY 1 cut(s) 412
EcoRII CCWGG 5 cut(s) 63, 408, 626, 643, 1173
EcoT14I CCWWGG 1 cut(s) 1099
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 1099
FaeI CATG 7 cut(s) 108, 351, 379, 769, 1114, 1171, 1313
FaqI GGGAC 2 cut(s) 56, 661
FatI CATG 7 cut(s) 104, 347, 375, 765, 1110, 1167, 1309
FauI CCCGC 1 cut(s) 109
FauNDI CATATG 2 cut(s) 151, 844
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 4 cut(s) 423, 585, 1054, 1260
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 4 cut(s) 423, 585, 1054, 1260
FspBI CTAG 1 cut(s) 1061
GluI GCNGC 4 cut(s) 423, 585, 1054, 1260
GsaI CCCAGC 2 cut(s) 161, 548
HaeIII GGCC 4 cut(s) 28, 63, 408, 413
HapII CCGG 3 cut(s) 168, 714, 890
HgaI GACGC 1 cut(s) 1225
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 7 cut(s) 108, 351, 379, 769, 1114, 1171, 1313
HindIII AAGCTT 2 cut(s) 263, 333
HinfI GANTC 6 cut(s) 20, 101, 138, 612, 1114, 1295
HpaII CCGG 3 cut(s) 168, 714, 890
HphI GGTGA 1 cut(s) 910
Hpy166II GTNNAC 2 cut(s) 792, 1069
Hpy188I TCNGA 5 cut(s) 90, 189, 255, 296, 1037
Hpy188III TCNNGA 7 cut(s) 168, 376, 562, 725, 980, 1021, 1111
Hpy8I GTNNAC 2 cut(s) 792, 1069
HpyAV CCTTC 3 cut(s) 895, 934, 1180
HpyCH4III ACNGT 5 cut(s) 146, 175, 559, 742, 1045
HpyCH4IV ACGT 1 cut(s) 69
HpyF10VI GCNNNNNNNGC 5 cut(s) 410, 419, 503, 1242, 1259
HpyF3I CTNAG 2 cut(s) 188, 533
HpySE526I ACGT 1 cut(s) 69
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 7 cut(s) 108, 351, 379, 769, 1114, 1171, 1313
Kpn2I TCCGGA 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 4 cut(s) 304, 378, 448, 1321
LmnI GCTCC 2 cut(s) 680, 1124
Lsp1109I GCAGC 3 cut(s) 409, 596, 1271
MabI ACCWGGT 1 cut(s) 1173
MaeI CTAG 1 cut(s) 1061
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 820
MalI GATC 4 cut(s) 306, 380, 450, 1323
MboI GATC 4 cut(s) 304, 378, 448, 1321
MboII GAAGA 2 cut(s) 62, 1195
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 2 cut(s) 675, 1038
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 1123
MmeI TCCRAC 1 cut(s) 173
MnlI CCTC 8 cut(s) 195, 272, 279, 425, 738, 1043, 1152, 1272
Mox20I TGGCCA 1 cut(s) 408
MroI TCCGGA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 408
MseI TTAA 4 cut(s) 366, 603, 999, 1265
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 3 cut(s) 168, 714, 890
MspR9I CCNGG 5 cut(s) 65, 410, 628, 645, 1175
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 3 cut(s) 704, 723, 1010
MvaI CCWGG 5 cut(s) 65, 410, 628, 645, 1175
MwoI GCNNNNNNNGC 5 cut(s) 410, 419, 503, 1242, 1259
NdeI CATATG 2 cut(s) 151, 844
NdeII GATC 4 cut(s) 304, 378, 448, 1321
NlaIII CATG 7 cut(s) 108, 351, 379, 769, 1114, 1171, 1313
NlaIV GGNNCC 3 cut(s) 28, 183, 1126
NspI RCATGY 2 cut(s) 769, 1171
PagI TCATGA 2 cut(s) 375, 1110
PasI CCCWGGG 2 cut(s) 627, 644
PciI ACATGT 1 cut(s) 1167
PctI GAATGC 3 cut(s) 704, 723, 1010
PfeI GAWTC 5 cut(s) 20, 101, 138, 612, 1295
PflMI CCANNNNNTGG 1 cut(s) 1100
PinAI ACCGGT 1 cut(s) 889
PkrI GCNGC 4 cut(s) 424, 586, 1055, 1261
PleI GAGTC 1 cut(s) 1122
PpsI GAGTC 1 cut(s) 1122
PscI ACATGT 1 cut(s) 1167
Psp6I CCWGG 5 cut(s) 63, 408, 626, 643, 1173
PspFI CCCAGC 2 cut(s) 157, 544
PspGI CCWGG 5 cut(s) 63, 408, 626, 643, 1173
PspN4I GGNNCC 3 cut(s) 28, 183, 1126
PspOMI GGGCCC 1 cut(s) 26
PspPI GGNCC 6 cut(s) 26, 27, 61, 412, 592, 887
PsrI GAACNNNNNNTAC 2 cut(s) 773, 805
PsuI RGATCY 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 2 cut(s) 782, 1172
RsaNI GTAC 2 cut(s) 781, 1171
SaqAI TTAA 4 cut(s) 366, 603, 999, 1265
SatI GCNGC 4 cut(s) 423, 585, 1054, 1260
Sau3AI GATC 4 cut(s) 304, 378, 448, 1321
Sau96I GGNCC 6 cut(s) 26, 27, 61, 412, 592, 887
SchI GAGTC 1 cut(s) 1123
ScrFI CCNGG 5 cut(s) 65, 410, 628, 645, 1175
SduI GDGCHC 1 cut(s) 30
SexAI ACCWGGT 1 cut(s) 1173
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 2 cut(s) 592, 887
SmlI CTYRAG 3 cut(s) 416, 562, 1276
SmoI CTYRAG 3 cut(s) 416, 562, 1276
Sse9I AATT 2 cut(s) 675, 1038
SsiI CCGC 4 cut(s) 81, 116, 201, 1053
SspI AATATT 2 cut(s) 666, 1231
SspMI CTAG 1 cut(s) 1061
StyD4I CCNGG 5 cut(s) 63, 408, 626, 643, 1173
StyI CCWWGG 1 cut(s) 1099
TaaI ACNGT 5 cut(s) 146, 175, 559, 742, 1045
TaiI ACGT 1 cut(s) 72
TaqI TCGA 2 cut(s) 18, 45
TasI AATT 2 cut(s) 675, 1038
TatI WGTACW 1 cut(s) 780
TauI GCSGC 1 cut(s) 1056
TfiI GAWTC 5 cut(s) 20, 101, 138, 612, 1295
Tru1I TTAA 4 cut(s) 366, 603, 999, 1265
Tru9I TTAA 4 cut(s) 366, 603, 999, 1265
TscAI CASTG 6 cut(s) 151, 232, 391, 594, 747, 759
TseI GCWGC 3 cut(s) 422, 584, 1259
TspDTI ATGAA 9 cut(s) 17, 63, 93, 257, 364, 470, 1099, 1141, 1191
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 6 cut(s) 151, 232, 391, 594, 747, 759
Van91I CCANNNNNTGG 1 cut(s) 1100
VpaK11BI GGWCC 2 cut(s) 592, 887
XceI RCATGY 2 cut(s) 769, 1171
XspI CTAG 1 cut(s) 1061
ZraI GACGTC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.