FvH4_7g27620

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007067
Physical Location & Seq
Forward (+)
20584490 .. 20589489
5000 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27620.t1

Sequence Viewer

Length: 1158 bp
ATGGACCTGAAGTCAAACCATATTTCTCCTGTTCTCACTGATCCTGCGCCTTTAGGCAAGTCAAGACTTGGTGTACATTCCAGTCTGTTATCATATTCACCTCCTGGAACAGCTTTTCCAGCAGACCTTCTCTTAACCATCCCTAGGAGGAAGAATGCATTTCTTGATGATGTTCGCTCTAGCTCTTGGCTTGATGCTATGAAATCTTCATCACCTCCTCACAGAAAGATAACTAAGGATGTTAACAATGACTCTGCAGCAACTGAGTATGATATTGCCTATCGCACATGGATGGTTAAGTATCCGTCAGCTCTTACATCCTTTGAGCAAATCTCTAATTACGCCAAGGGAAAGAGAATAGCATTGTTTCTCGATTATGATGGGACTCTTTCGCCAATTGTAGATAACCCTGACTGTGCCTTTATGTCTGATGCTATGCGAATGGCAGTAAAAAGAGTGGCAAAACATTTCCCAACAGCAATAATTAGTGGAAGAAGCCATGACAAGGTGTATGAGTTTGTTGGACTAAATGAACTATACTATGCGGGTAGTCATGGGATGGACATAATGGCCCCTGTTCTACAATCAGTAGTTGGTGACCATAGACATGGCTTTAGGTCTACTGACAAACAGGGCAACGATGTTAATGTGTTCCAGCCCGCTGCAGAGTTTTTACCTATGATTGGCGAGGTTCTTAAATCCCTAGAAGAAAGTACCAAAGACATTGAAGGATCTAAAGTTGAGAATAACAAGTTCTGCGTCTCTGTACACTACCGCAATGTAGATGAGAAGAATTGGGCTGTAGTTGCACAGCGGGTTCACGACATTCTTAAAAACTACCCACGCCTGCGTTTGACTCATGGACGGAAGGTTTTAGAGGTCCGACCAGTGATAAATTGGGATAAAGGGAAGGCAGTCACATTTTTACTTGAATCTCTAGGGCTAAGCAATTGTGACAATGTGCTCCCAATATATATTGGAGACGACCGCACAGATGAAGACGCATTCAAGGTTCTGAGGGAAGGAAACAAAGGTTATGGTATTTTAGTCTCTGCGGTGCCAAAGGAAAGCAATGCGTATTACTCACTAAGGGACCCCTCAGAGGTAATGGAGTTTCTCAAGTCACTGGTGCTATGGAGGAAGTCAAGTGCTCTATAA

Protein Analysis

386

Amino Acids

43.13

Weight (kDa)

8.22

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 123 - 367 1.3e-71 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1057
AccI GTMKAC 1 cut(s) 620
AciI CCGC 6 cut(s) 545, 660, 775, 814, 988, 1055
AclWI GGATC 2 cut(s) 35, 739
AcuI CTGAAG 1 cut(s) 29
AfaI GTAC 3 cut(s) 75, 715, 768
AfiI CCNNNNNNNGG 4 cut(s) 144, 505, 683, 1102
AgsI TTSAA 3 cut(s) 728, 932, 1009
AhdI GACNNNNNGTC 1 cut(s) 10
AjnI CCWGG 1 cut(s) 103
AloI GAACNNNNNNTCC 2 cut(s) 100, 132
AluBI AGCT 3 cut(s) 113, 183, 311
AluI AGCT 3 cut(s) 113, 183, 311
Alw21I GWGCWC 2 cut(s) 966, 1153
Alw26I GTCTC 3 cut(s) 766, 975, 1054
AlwI GGATC 2 cut(s) 35, 739
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 1 cut(s) 570
ApeKI GCWGC 2 cut(s) 257, 662
AspA2I CCTAGG 1 cut(s) 143
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 4 cut(s) 4, 571, 880, 1093
AsuHPI GGTGA 3 cut(s) 90, 204, 608
AvaII GGWCC 3 cut(s) 4, 880, 1093
AvrII CCTAGG 1 cut(s) 143
BanI GGYRCC 1 cut(s) 1057
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 2 cut(s) 966, 1153
BbvI GCAGC 2 cut(s) 269, 649
BccI CCATC 4 cut(s) 146, 286, 374, 553
BciT130I CCWGG 1 cut(s) 105
BciVI GTATCC 1 cut(s) 312
BcoDI GTCTC 3 cut(s) 766, 975, 1054
BfaI CTAG 4 cut(s) 144, 180, 704, 938
BfmI CTRYAG 3 cut(s) 255, 663, 801
BfuI GTATCC 1 cut(s) 312
BisI GCNGC 2 cut(s) 258, 663
BlnI CCTAGG 1 cut(s) 143
BlpI GCTNAGC 1 cut(s) 944
BlsI GCNGC 2 cut(s) 259, 664
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 3 cut(s) 4, 880, 1093
BmeRI GACNNNNNGTC 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 4, 571, 880, 1093
BmiI GGNNCC 4 cut(s) 573, 1059, 1094, 1095
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 2 cut(s) 184, 421
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu1102I GCTNAGC 1 cut(s) 944
BpuEI CTTGAG 1 cut(s) 1103
BsaJI CCNNGG 2 cut(s) 143, 345
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 505, 683, 1102
Bse1I ACTGG 3 cut(s) 81, 887, 1131
Bse3DI GCAATG 2 cut(s) 784, 1078
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 143, 345
BseGI GGATG 5 cut(s) 138, 244, 297, 317, 564
BseLI CCNNNNNNNGG 4 cut(s) 144, 505, 683, 1102
BseMI GCAATG 2 cut(s) 784, 1078
BseMII CTCAG 3 cut(s) 255, 1007, 1113
BseNI ACTGG 3 cut(s) 81, 887, 1131
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 2 cut(s) 269, 649
Bsh1285I CGRYCG 1 cut(s) 988
BshFI GGCC 1 cut(s) 572
BshNI GGYRCC 1 cut(s) 1057
BsiEI CGRYCG 1 cut(s) 988
BsiHKAI GWGCWC 2 cut(s) 966, 1153
BslFI GGGAC 2 cut(s) 397, 1106
BslI CCNNNNNNNGG 4 cut(s) 144, 505, 683, 1102
BsmAI GTCTC 3 cut(s) 766, 975, 1054
BsmBI CGTCTC 2 cut(s) 766, 975
BsmFI GGGAC 2 cut(s) 397, 1106
BsmI GAATGC 2 cut(s) 160, 1004
BsnI GGCC 1 cut(s) 572
Bsp1286I GDGCHC 2 cut(s) 966, 1153
Bsp1407I TGTACA 2 cut(s) 73, 766
Bsp143I GATC 2 cut(s) 40, 731
Bsp1720I GCTNAGC 1 cut(s) 944
BspACI CCGC 6 cut(s) 545, 660, 775, 814, 988, 1055
BspANI GGCC 1 cut(s) 572
BspCNI CTCAG 3 cut(s) 256, 1008, 1112
BspLI GGNNCC 4 cut(s) 573, 1059, 1094, 1095
BspMAI CTGCAG 2 cut(s) 259, 667
BspPI GGATC 2 cut(s) 35, 739
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 2 cut(s) 784, 1078
BsrGI TGTACA 2 cut(s) 73, 766
BsrI ACTGG 3 cut(s) 81, 887, 1131
BssECI CCNNGG 2 cut(s) 143, 345
BssMI GATC 2 cut(s) 40, 731
BssT1I CCWWGG 2 cut(s) 143, 345
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 416
BstAUI TGTACA 2 cut(s) 73, 766
BstC8I GCNNGC 2 cut(s) 660, 848
BstDEI CTNAG 6 cut(s) 234, 264, 944, 1016, 1088, 1099
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 5 cut(s) 138, 244, 297, 317, 564
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 2 cut(s) 43, 734
BstMAI GTCTC 3 cut(s) 766, 975, 1054
BstMBI GATC 2 cut(s) 40, 731
BstMCI CGRYCG 1 cut(s) 988
BstMWI GCNNNNNNNGC 2 cut(s) 119, 806
BstNI CCWGG 1 cut(s) 105
BstPI GGTNACC 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 3 cut(s) 255, 663, 801
BstV1I GCAGC 2 cut(s) 269, 649
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 1 cut(s) 731
BstXI CCANNNNNNTGG 1 cut(s) 608
BstYI RGATCY 1 cut(s) 731
BsuI GTATCC 1 cut(s) 312
BsuRI GGCC 1 cut(s) 572
BtsCI GGATG 5 cut(s) 138, 244, 297, 317, 564
BtsIMutI CAGTG 3 cut(s) 36, 894, 1124
Cac8I GCNNGC 2 cut(s) 660, 848
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 4 cut(s) 4, 571, 880, 1093
CseI GACGC 2 cut(s) 748, 1010
Csp6I GTAC 3 cut(s) 74, 714, 767
CviAII CATG 5 cut(s) 288, 500, 554, 608, 860
CviQI GTAC 3 cut(s) 74, 714, 767
DdeI CTNAG 6 cut(s) 234, 264, 944, 1016, 1088, 1099
DpnI GATC 2 cut(s) 42, 733
DpnII GATC 2 cut(s) 40, 731
DriI GACNNNNNGTC 1 cut(s) 10
Eam1105I GACNNNNNGTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 143, 345
Eco47I GGWCC 3 cut(s) 4, 880, 1093
Eco57I CTGAAG 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 596
EcoO109I RGGNCCY 1 cut(s) 1093
EcoO65I GGTNACC 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 143, 345
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 143, 345
Esp3I CGTCTC 2 cut(s) 766, 975
FaeI CATG 5 cut(s) 291, 503, 557, 611, 863
FaqI GGGAC 2 cut(s) 397, 1106
FatI CATG 5 cut(s) 287, 499, 553, 607, 859
FauI CCCGC 3 cut(s) 538, 667, 807
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 2 cut(s) 258, 663
FokI GGATG 5 cut(s) 125, 251, 304, 304, 571
Fsp4HI GCNGC 2 cut(s) 258, 663
FspBI CTAG 4 cut(s) 144, 180, 704, 938
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 258, 663
HaeIII GGCC 1 cut(s) 572
HgaI GACGC 2 cut(s) 748, 1010
HhaI GCGC 1 cut(s) 49
Hin1II CATG 5 cut(s) 291, 503, 557, 611, 863
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 4 cut(s) 251, 385, 856, 932
HpaI GTTAAC 1 cut(s) 244
HphI GGTGA 3 cut(s) 90, 204, 608
Hpy166II GTNNAC 5 cut(s) 74, 244, 621, 769, 820
Hpy188I TCNGA 4 cut(s) 430, 884, 1017, 1102
Hpy188III TCNNGA 4 cut(s) 63, 164, 371, 821
Hpy8I GTNNAC 5 cut(s) 74, 244, 621, 769, 820
HpyAV CCTTC 5 cut(s) 137, 722, 862, 904, 1016
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4V TGCA 4 cut(s) 158, 257, 665, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 806
HpyF3I CTNAG 6 cut(s) 234, 264, 944, 1016, 1088, 1099
Hsp92II CATG 5 cut(s) 291, 503, 557, 611, 863
HspAI GCGC 1 cut(s) 47
KflI GGGWCCC 1 cut(s) 1093
KspAI GTTAAC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 40, 731
LmnI GCTCC 1 cut(s) 969
Lsp1109I GCAGC 2 cut(s) 269, 649
LweI GCATC 2 cut(s) 184, 421
MaeI CTAG 4 cut(s) 144, 180, 704, 938
MaeIII GTNAC 4 cut(s) 596, 916, 953, 1122
MalI GATC 2 cut(s) 42, 733
MboI GATC 2 cut(s) 40, 731
MboII GAAGA 6 cut(s) 163, 198, 504, 719, 802, 1010
MfeI CAATTG 2 cut(s) 396, 949
MflI RGATCY 1 cut(s) 731
MhlI GDGCHC 2 cut(s) 966, 1153
MluCI AATT 6 cut(s) 337, 396, 483, 793, 895, 949
MlyI GAGTC 3 cut(s) 245, 379, 850
MmeI TCCRAC 2 cut(s) 502, 907
Mph1103I ATGCAT 1 cut(s) 160
MseI TTAA 6 cut(s) 134, 243, 297, 645, 696, 831
MslI CAYNNNNRTG 2 cut(s) 290, 606
MspA1I CMGCKG 2 cut(s) 662, 814
MspR9I CCNGG 1 cut(s) 105
MunI CAATTG 2 cut(s) 396, 949
Mva1269I GAATGC 2 cut(s) 160, 1004
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 119, 806
NdeII GATC 2 cut(s) 40, 731
NlaIII CATG 5 cut(s) 291, 503, 557, 611, 863
NlaIV GGNNCC 4 cut(s) 573, 1059, 1094, 1095
NmuCI GTSAC 4 cut(s) 596, 916, 953, 1122
NsiI ATGCAT 1 cut(s) 160
PctI GAATGC 2 cut(s) 160, 1004
PfeI GAWTC 1 cut(s) 932
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 2 cut(s) 259, 664
PleI GAGTC 3 cut(s) 245, 379, 850
PpsI GAGTC 3 cut(s) 245, 379, 850
PpuMI RGGWCCY 1 cut(s) 1093
Psp5II RGGWCCY 1 cut(s) 1093
Psp6I CCWGG 1 cut(s) 103
PspEI GGTNACC 1 cut(s) 596
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 4 cut(s) 573, 1059, 1094, 1095
PspPI GGNCC 4 cut(s) 4, 571, 880, 1093
PspPPI RGGWCCY 1 cut(s) 1093
PstI CTGCAG 2 cut(s) 259, 667
PstNI CAGNNNCTG 1 cut(s) 263
PsuI RGATCY 1 cut(s) 731
RsaI GTAC 3 cut(s) 75, 715, 768
RsaNI GTAC 3 cut(s) 74, 714, 767
RseI CAYNNNNRTG 2 cut(s) 290, 606
SaqAI TTAA 6 cut(s) 134, 243, 297, 645, 696, 831
SatI GCNGC 2 cut(s) 258, 663
Sau3AI GATC 2 cut(s) 40, 731
Sau96I GGNCC 4 cut(s) 4, 571, 880, 1093
SchI GAGTC 3 cut(s) 245, 379, 850
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 2 cut(s) 966, 1153
SfaNI GCATC 2 cut(s) 184, 421
SfcI CTRYAG 3 cut(s) 255, 663, 801
SinI GGWCC 3 cut(s) 4, 880, 1093
SmiMI CAYNNNNRTG 2 cut(s) 290, 606
SmlI CTYRAG 1 cut(s) 1118
SmoI CTYRAG 1 cut(s) 1118
Sse9I AATT 6 cut(s) 337, 396, 483, 793, 895, 949
SsiI CCGC 6 cut(s) 545, 660, 775, 814, 988, 1055
SspMI CTAG 4 cut(s) 144, 180, 704, 938
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 2 cut(s) 143, 345
TaaI ACNGT 1 cut(s) 416
TaqI TCGA 1 cut(s) 372
TasI AATT 6 cut(s) 337, 396, 483, 793, 895, 949
TatI WGTACW 2 cut(s) 73, 766
TfiI GAWTC 1 cut(s) 932
Tru1I TTAA 6 cut(s) 134, 243, 297, 645, 696, 831
Tru9I TTAA 6 cut(s) 134, 243, 297, 645, 696, 831
TscAI CASTG 3 cut(s) 43, 894, 1131
TseFI GTSAC 4 cut(s) 596, 916, 953, 1122
TseI GCWGC 2 cut(s) 257, 662
Tsp45I GTSAC 4 cut(s) 596, 916, 953, 1122
TspDTI ATGAA 4 cut(s) 198, 215, 546, 1011
TspGWI ACGGA 2 cut(s) 294, 880
TspRI CASTG 3 cut(s) 43, 894, 1131
VpaK11BI GGWCC 3 cut(s) 4, 880, 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 894
XmaJI CCTAGG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 620
XspI CTAG 4 cut(s) 144, 180, 704, 938
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.