Rh1DG389500

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007067
Physical Location & Seq
Forward (+)
60830169 .. 60836039
5871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG389500.1

Sequence Viewer

Length: 1152 bp
ATGGACCTGAAATCAAACCATACTTCTCCTGTTCTCACTGATCCTGCGCCTTTAGGCAAGTCGAGACTTGGCGTACATTCCAGTCTGTTAACATATTCAGGGTCAGGAACAGCTTTTCCAGCACTCTTAACCATCCCTAGGAGGAAGAATGGATTTCTTGATGATGTTCGCTCCAGCTCTTGGCTTGATGCTATGAAATCTTCATCGCCTCCTCACAGAAAGATAACTAAGGATGTTAACAATGACTCTCCAGCAACTGAGTATGATATTGCCTATCGCACATGGATGGTTAAGTATCCATCAGCTCTTACATCTTTTGAGCAAATCACTAATTATGCCAAGGGCAAAAGAATAGCATTGTTTCTCGATTATGATGGGACTCTTTCGCCAATTGTAGATAACCCTGACTGTGCCTTTATGTCTGATGCTATGCGAAGGGCTGTAAGAAGAGTGGCAAAACATTTCCCAACAGCAATAATTAGTGGAAGAAGCCATGACAAGGTATACGAGTTTGTTGGACTAAATGAACTATATTATGCGGGTAGTCATGGGATGGACATAATGGGCCCTGTTAGACAATCAGTAGTTGGTGACCATAGACATGGCTTTCGGTCTACTGACAGACAGGGCAAGGATGTTAATCTGTTCCAGCCTGCAGCAGAGTTTTTACCTATGATTGGCGAGGTTCTTAAATCCCTAGTAGAAACTACCAAAGACATTGAAGGAGCTAAAGTTGAGAATAACAAGTTCTGTGTCTCTGTACACTACCGCAATGTAGATGAGAAGTATTGGGCTGTAGTTGCACAGCGTGTTCATGACATTCTTAAAAACTACCCACGCCTGCGGTTGACTCATGGACGGAAGGTTTTAGAGGTCCGACCAGTGATAAATTGGGATAAAGGGAAGGCAGTCACATTTTTACTTGAATCTCTTGGGCTAAGTGATTGTGAAGATGTGCTCCCAATATATATTGGAGATGACCGCACAGATGAAGATGCATTCAAGGTTCTGAGGGAAGGAAACAGGGGTTATGGCATTTTAGTCTCTGCTGTGCCAAAGGAAAGCAATGCATTTTACTCACTAAGGGACCCCTCAGAGGTGATGGAGTTTCTGAAGTCACTGGTGATATGGAGGAAGTCAAGTGCTCTATAA

Protein Analysis

383

Amino Acids

43.06

Weight (kDa)

8.87

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 121 - 365 6.2e-71 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AccI GTMKAC 2 cut(s) 504, 614
AciI CCGC 4 cut(s) 539, 769, 844, 982
AclWI GGATC 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 1133
AdeI CACNNNGTG 1 cut(s) 809
AfaI GTAC 2 cut(s) 75, 762
AfiI CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
AgsI TTSAA 3 cut(s) 722, 926, 1003
AloI GAACNNNNNNTCC 2 cut(s) 100, 132
AluBI AGCT 4 cut(s) 113, 177, 305, 728
AluI AGCT 4 cut(s) 113, 177, 305, 728
Alw21I GWGCWC 2 cut(s) 960, 1147
Alw26I GTCTC 3 cut(s) 58, 760, 1048
AlwI GGATC 1 cut(s) 35
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 1 cut(s) 565
ApaI GGGCCC 1 cut(s) 569
ApeKI GCWGC 1 cut(s) 656
ArsI GACNNNNNNTTYG 2 cut(s) 591, 623
AspA2I CCTAGG 1 cut(s) 137
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
AsuHPI GGTGA 3 cut(s) 602, 1111, 1135
AvaII GGWCC 3 cut(s) 4, 874, 1087
AvrII CCTAGG 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 569
BanII GRGCYC 1 cut(s) 569
Bbv12I GWGCWC 2 cut(s) 960, 1147
BbvI GCAGC 1 cut(s) 668
BccI CCATC 6 cut(s) 140, 280, 307, 368, 547, 1096
BciVI GTATCC 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 58, 760, 1048
BfaI CTAG 2 cut(s) 138, 698
BfmI CTRYAG 2 cut(s) 654, 795
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 1 cut(s) 657
BlnI CCTAGG 1 cut(s) 137
BlsI GCNGC 1 cut(s) 658
Bme18I GGWCC 3 cut(s) 4, 874, 1087
BmgT120I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
BmiI GGNNCC 3 cut(s) 567, 1088, 1089
BmsI GCATC 3 cut(s) 178, 415, 985
BpmI CTGGAG 2 cut(s) 157, 234
BsaBI GATNNNNATC 1 cut(s) 639
BsaJI CCNNGG 2 cut(s) 137, 339
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
Bse1I ACTGG 3 cut(s) 81, 881, 1125
Bse3DI GCAATG 2 cut(s) 778, 1072
Bse8I GATNNNNATC 1 cut(s) 639
BseDI CCNNGG 2 cut(s) 137, 339
BseGI GGATG 5 cut(s) 132, 238, 291, 558, 640
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
BseMI GCAATG 2 cut(s) 778, 1072
BseMII CTCAG 3 cut(s) 249, 1001, 1107
BseNI ACTGG 3 cut(s) 81, 881, 1125
BseRI GAGGAG 1 cut(s) 201
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 1 cut(s) 668
BshFI GGCC 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 960, 1147
BslFI GGGAC 2 cut(s) 391, 1100
BslI CCNNNNNNNGG 5 cut(s) 138, 180, 499, 677, 1096
BsmAI GTCTC 3 cut(s) 58, 760, 1048
BsmFI GGGAC 2 cut(s) 391, 1100
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 1 cut(s) 567
Bsp120I GGGCCC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 569, 960, 1147
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 4 cut(s) 539, 769, 844, 982
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 3 cut(s) 250, 1002, 1106
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 3 cut(s) 567, 1088, 1089
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 778, 1072
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 3 cut(s) 81, 881, 1125
BssECI CCNNGG 2 cut(s) 137, 339
BssMI GATC 1 cut(s) 40
BssNAI GTATAC 1 cut(s) 505
BssT1I CCWWGG 2 cut(s) 137, 339
Bst1107I GTATAC 1 cut(s) 505
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 1 cut(s) 442
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 2 cut(s) 654, 842
BstDEI CTNAG 6 cut(s) 228, 258, 938, 1010, 1082, 1093
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 5 cut(s) 132, 238, 291, 558, 640
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 3 cut(s) 58, 760, 1048
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 119, 800
BstPI GGTNACC 1 cut(s) 590
BstSFI CTRYAG 2 cut(s) 654, 795
BstSLI GKGCMC 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 668
BstXI CCANNNNNNTGG 1 cut(s) 602
BstZ17I GTATAC 1 cut(s) 505
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 1 cut(s) 567
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 5 cut(s) 132, 238, 291, 558, 640
BtsIMutI CAGTG 3 cut(s) 36, 888, 1118
Cac8I GCNNGC 2 cut(s) 654, 842
CaiI CAGNNNCTG 1 cut(s) 257
CciI TCATGA 1 cut(s) 814
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
Csp6I GTAC 2 cut(s) 74, 761
CviAII CATG 6 cut(s) 282, 494, 548, 602, 815, 854
CviQI GTAC 2 cut(s) 74, 761
DdeI CTNAG 6 cut(s) 228, 258, 938, 1010, 1082, 1093
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DraIII CACNNNGTG 1 cut(s) 809
Eam1104I CTCTTC 1 cut(s) 442
EarI CTCTTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 137, 339
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 3 cut(s) 4, 874, 1087
Eco57I CTGAAG 1 cut(s) 1133
Eco91I GGTNACC 1 cut(s) 590
EcoO109I RGGNCCY 2 cut(s) 566, 1087
EcoO65I GGTNACC 1 cut(s) 590
EcoT14I CCWWGG 2 cut(s) 137, 339
EcoT22I ATGCAT 2 cut(s) 1000, 1072
EcoT38I GRGCYC 1 cut(s) 569
ErhI CCWWGG 2 cut(s) 137, 339
FaeI CATG 6 cut(s) 285, 497, 551, 605, 818, 857
FaqI GGGAC 2 cut(s) 391, 1100
FatI CATG 6 cut(s) 281, 493, 547, 601, 814, 853
FauI CCCGC 1 cut(s) 532
FblI GTMKAC 2 cut(s) 504, 614
Fnu4HI GCNGC 1 cut(s) 657
FokI GGATG 5 cut(s) 119, 245, 298, 565, 647
FriOI GRGCYC 1 cut(s) 569
Fsp4HI GCNGC 1 cut(s) 657
FspBI CTAG 2 cut(s) 138, 698
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 657
GsuI CTGGAG 2 cut(s) 157, 234
HaeIII GGCC 1 cut(s) 567
HhaI GCGC 1 cut(s) 49
Hin1II CATG 6 cut(s) 285, 497, 551, 605, 818, 857
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 3 cut(s) 90, 238, 849
HindII GTYRAC 3 cut(s) 90, 238, 849
HinfI GANTC 4 cut(s) 245, 379, 850, 926
HpaI GTTAAC 2 cut(s) 90, 238
HphI GGTGA 3 cut(s) 602, 1111, 1135
Hpy166II GTNNAC 6 cut(s) 90, 238, 505, 615, 763, 849
Hpy188I TCNGA 5 cut(s) 424, 878, 1011, 1096, 1113
Hpy188III TCNNGA 5 cut(s) 63, 105, 158, 365, 815
Hpy8I GTNNAC 6 cut(s) 90, 238, 505, 615, 763, 849
HpyAV CCTTC 5 cut(s) 429, 716, 856, 898, 1010
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 4 cut(s) 656, 803, 998, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 800
HpyF3I CTNAG 6 cut(s) 228, 258, 938, 1010, 1082, 1093
Hsp92II CATG 6 cut(s) 285, 497, 551, 605, 818, 857
HspAI GCGC 1 cut(s) 47
KflI GGGWCCC 1 cut(s) 1087
KspAI GTTAAC 2 cut(s) 90, 238
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 176, 725, 963
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 3 cut(s) 178, 415, 985
MaeI CTAG 2 cut(s) 138, 698
MaeIII GTNAC 3 cut(s) 590, 910, 1116
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 6 cut(s) 157, 192, 459, 498, 962, 1004
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 3 cut(s) 569, 960, 1147
MluCI AATT 4 cut(s) 331, 390, 477, 889
MlyI GAGTC 3 cut(s) 239, 373, 844
MmeI TCCRAC 2 cut(s) 496, 901
MnlI CCTC 9 cut(s) 135, 219, 222, 676, 865, 1005, 1090, 1102, 1125
Mph1103I ATGCAT 2 cut(s) 1000, 1072
MseI TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
MslI CAYNNNNRTG 2 cut(s) 284, 600
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 1 cut(s) 998
MwoI GCNNNNNNNGC 2 cut(s) 119, 800
NdeII GATC 1 cut(s) 40
NlaIII CATG 6 cut(s) 285, 497, 551, 605, 818, 857
NlaIV GGNNCC 3 cut(s) 567, 1088, 1089
NmuCI GTSAC 3 cut(s) 590, 910, 1116
NsiI ATGCAT 2 cut(s) 1000, 1072
PagI TCATGA 1 cut(s) 814
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 1 cut(s) 926
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 1 cut(s) 658
PleI GAGTC 3 cut(s) 239, 373, 844
PpsI GAGTC 3 cut(s) 239, 373, 844
PpuMI RGGWCCY 1 cut(s) 1087
Psp5II RGGWCCY 1 cut(s) 1087
PspEI GGTNACC 1 cut(s) 590
PspN4I GGNNCC 3 cut(s) 567, 1088, 1089
PspOMI GGGCCC 1 cut(s) 565
PspPI GGNCC 5 cut(s) 4, 565, 566, 874, 1087
PspPPI RGGWCCY 1 cut(s) 1087
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 75, 762
RsaNI GTAC 2 cut(s) 74, 761
RseI CAYNNNNRTG 2 cut(s) 284, 600
SaqAI TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
SatI GCNGC 1 cut(s) 657
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 5 cut(s) 4, 565, 566, 874, 1087
SchI GAGTC 3 cut(s) 239, 373, 844
SduI GDGCHC 3 cut(s) 569, 960, 1147
SfaNI GCATC 3 cut(s) 178, 415, 985
SfcI CTRYAG 2 cut(s) 654, 795
SinI GGWCC 3 cut(s) 4, 874, 1087
SmiMI CAYNNNNRTG 2 cut(s) 284, 600
Sse9I AATT 4 cut(s) 331, 390, 477, 889
SsiI CCGC 4 cut(s) 539, 769, 844, 982
SspMI CTAG 2 cut(s) 138, 698
StyI CCWWGG 2 cut(s) 137, 339
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 2 cut(s) 62, 366
TaqII GACCGA 1 cut(s) 600
TasI AATT 4 cut(s) 331, 390, 477, 889
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 1 cut(s) 926
Tru1I TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
Tru9I TTAA 7 cut(s) 89, 128, 237, 291, 639, 690, 825
TscAI CASTG 3 cut(s) 43, 888, 1125
TseFI GTSAC 3 cut(s) 590, 910, 1116
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 3 cut(s) 590, 910, 1116
TspDTI ATGAA 5 cut(s) 192, 209, 540, 803, 1005
TspGWI ACGGA 1 cut(s) 874
TspRI CASTG 3 cut(s) 43, 888, 1125
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 3 cut(s) 4, 874, 1087
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XmaJI CCTAGG 1 cut(s) 137
XmiI GTMKAC 2 cut(s) 504, 614
XspI CTAG 2 cut(s) 138, 698
Zsp2I ATGCAT 2 cut(s) 1000, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.