AT5G53140

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005995
Physical Location & Seq
Forward (+)
21548397 .. 21552594
4198 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53140.2

Sequence Viewer

Length: 1263 bp
ATGGTATGCAGCAGTTTCATAAGGAGTTTCATTGTTCAAGCTGGTTGTCGTATTGGGGTGTTGGCTCAAGGAAGACATCAGTTTATTCATATTAAGAAGACTTTGAGTGTGGGTTTTGGATTTAGAACTAGTGTGATTGGATTTAGAACAACCAGTGGAATTGGATTTAGAACTAGTGCGAAGATGATGGTTGATACTTCTGCTGGAGAGAAACGTATATCCTTAGTTGATATGCCGCCTGAGAAAGTTGATGATGGTGGATACATCGGTGGTGGGTGGAAAAATGATGATGGAAGCTTGAGCTGTGGGTACTGTAGTTTCAGAGGGAAAAGATCTACGATGGAAGATTTCTATGATATCAAAGCTTCCACTATTGAAGGCCAAGCAGTTTGCATGTTTGGAATATTTGATGGCCATGGTGGTTCACGTGCTGCTGAATACCTGAAGGAACACCTGTTTAACAATCTCATGAAGCATCCACAATTTTTGACAGACACCAAGCTGGCATTAAATGAAACATATAAACAAACTGATGTAGCATTCCTCGAGTCCGAAAAGGATACTTACAGAGATGATGGCTCCACAGCATCTGCTGCTGTGTTGGTGGGGAACCATTTGTATGTTGCAAATGTTGGAGACTCGAGGACAATAGTTTCTAAAGCTGGGAAAGCGATCGCGCTATCTGATGACCATAAGCCAAATAGAAGCGATGAAAGAAAGCGAATTGAAAGTGCTGGTGGTGTTATCATGTGGGCAGGAACATGGAGAGTAGGTGGGGTGTTGGCTATGTCCCGGGCCTTTGGTAACAGAATGCTGAAGCAATTCGTTGTTGCTGAACCCGAGATACAAGATCTAGAGATAGATCATGAGGCCGAGTTGCTTGTGCTTGCAAGTGACGGTTTATGGGATGTGGTACCAAATGAGGATGCGGTAGCCCTTGCTCAGAGCGAGGAAGAGCCCGAAGCAGCTGCCCGCAAGTTAACTGACACTGCCTTCAGCCGTGGCAGTGCAGACAACATCACGTGCATTGTTGTTAAATTCCGTCATGATAAGACAGAATCTCCTAAAATCGAAACAAACGCCATGGCTGAATCGGAACCTGAACTGAACCCCACAACTGAACTGGAACCTGAATCAAACCCCAGTACTGCATTGGAAACTGAATCAATCCCCAAAGCTGAACTGGAATCCGAACCTGATGCTATACCTGATCCAAAACCTGAAACCGAACCAGAGACCAAGGGTGAGAAAGCTGGTGAGTAA

Protein Analysis

420

Amino Acids

45.79

Weight (kDa)

5.0

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 102 - 340 3e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 913
AccB1I GGYRCC 1 cut(s) 913
AccII CGCG 1 cut(s) 677
AciI CCGC 3 cut(s) 236, 929, 973
AclWI GGATC 1 cut(s) 1205
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 1 cut(s) 1037
AcuI CTGAAG 3 cut(s) 464, 836, 979
AcvI CACGTG 2 cut(s) 428, 1023
AfaI GTAC 3 cut(s) 311, 915, 1147
AgsI TTSAA 3 cut(s) 38, 377, 728
AhlI ACTAGT 2 cut(s) 128, 173
AluBI AGCT 9 cut(s) 41, 297, 303, 365, 502, 662, 968, 1178, 1253
AluI AGCT 9 cut(s) 41, 297, 303, 365, 502, 662, 968, 1178, 1253
Alw26I GTCTC 2 cut(s) 630, 1229
AlwI GGATC 1 cut(s) 1205
AlwNI CAGNNNCTG 1 cut(s) 590
Ama87I CYCGRG 4 cut(s) 545, 640, 792, 839
AoxI GGCC 4 cut(s) 379, 412, 795, 870
ApeKI GCWGC 5 cut(s) 9, 431, 593, 965, 968
ApoI RAATTY 1 cut(s) 1037
AsiSI GCGATCGC 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 821
Asp718I GGTACC 1 cut(s) 913
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 1 cut(s) 795
AsuC2I CCSGG 2 cut(s) 793, 794
AsuHPI GGTGA 1 cut(s) 1256
AvaI CYCGRG 4 cut(s) 545, 640, 792, 839
BaeI ACNNNNGTAYC 4 cut(s) 253, 286, 301, 334
BalI TGGCCA 1 cut(s) 414
BanI GGYRCC 1 cut(s) 913
BanII GRGCYC 1 cut(s) 960
BbrPI CACGTG 2 cut(s) 428, 1023
BbsI GAAGAC 2 cut(s) 79, 104
BbvI GCAGC 5 cut(s) 21, 418, 580, 955, 977
BccI CCATC 6 cut(s) 181, 248, 284, 334, 404, 569
BceAI ACGGC 1 cut(s) 984
BcgI CGANNNNNNTGC 4 cut(s) 950, 984, 1181, 1215
BciVI GTATCC 2 cut(s) 254, 553
BcnI CCSGG 2 cut(s) 793, 794
BcoDI GTCTC 2 cut(s) 630, 1229
BcuI ACTAGT 2 cut(s) 128, 173
BfaI CTAG 3 cut(s) 129, 174, 854
BfmI CTRYAG 1 cut(s) 313
BfuI GTATCC 2 cut(s) 254, 553
BglII AGATCT 2 cut(s) 332, 850
BisI GCNGC 6 cut(s) 10, 236, 432, 594, 966, 969
BlsI GCNGC 6 cut(s) 11, 237, 433, 595, 967, 970
BmcAI AGTACT 1 cut(s) 1147
Bme1390I CCNGG 2 cut(s) 793, 794
BmeT110I CYCGRG 4 cut(s) 545, 640, 792, 839
BmgT120I GGNCC 1 cut(s) 795
BmiI GGNNCC 5 cut(s) 580, 611, 915, 1098, 1128
BmrFI CCNGG 2 cut(s) 793, 794
BmrI ACTGGG 1 cut(s) 1137
BmsI GCATC 4 cut(s) 484, 596, 916, 1189
BmuI ACTGGG 1 cut(s) 1137
BpiI GAAGAC 2 cut(s) 79, 104
BpmI CTGGAG 1 cut(s) 225
BpuEI CTTGAG 2 cut(s) 51, 319
BpuMI CCSGG 2 cut(s) 793, 794
BsaAI YACGTR 2 cut(s) 428, 1023
BsaI GGTCTC 1 cut(s) 1229
BsaJI CCNNGG 5 cut(s) 415, 792, 1000, 1083, 1239
BsaXI ACNNNNNCTCC 4 cut(s) 757, 787, 1045, 1075
Bse1I ACTGG 4 cut(s) 153, 1128, 1143, 1188
BseDI CCNNGG 5 cut(s) 415, 792, 1000, 1083, 1239
BseGI GGATG 3 cut(s) 475, 913, 931
BseMII CTCAG 2 cut(s) 231, 956
BseNI ACTGG 4 cut(s) 153, 1128, 1143, 1188
BseXI GCAGC 5 cut(s) 21, 418, 580, 955, 977
BseYI CCCAGC 1 cut(s) 662
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 1 cut(s) 677
Bsh1285I CGRYCG 1 cut(s) 675
BshFI GGCC 4 cut(s) 381, 414, 797, 872
BshNI GGYRCC 1 cut(s) 913
BsiEI CGRYCG 1 cut(s) 675
BsiHKCI CYCGRG 4 cut(s) 545, 640, 792, 839
BsiSI CCGG 1 cut(s) 793
BslFI GGGAC 1 cut(s) 775
BsmAI GTCTC 2 cut(s) 630, 1229
BsmFI GGGAC 1 cut(s) 775
BsmI GAATGC 2 cut(s) 539, 816
BsnI GGCC 4 cut(s) 381, 414, 797, 872
Bso31I GGTCTC 1 cut(s) 1229
BsoBI CYCGRG 4 cut(s) 545, 640, 792, 839
Bsp1286I GDGCHC 1 cut(s) 960
Bsp143I GATC 5 cut(s) 332, 672, 850, 862, 1210
Bsp19I CCATGG 2 cut(s) 415, 1083
BspACI CCGC 3 cut(s) 236, 929, 973
BspANI GGCC 4 cut(s) 381, 414, 797, 872
BspCNI CTCAG 2 cut(s) 232, 955
BspFNI CGCG 1 cut(s) 677
BspHI TCATGA 3 cut(s) 468, 865, 1045
BspLI GGNNCC 5 cut(s) 580, 611, 915, 1098, 1128
BspPI GGATC 1 cut(s) 1205
BspQI GCTCTTC 1 cut(s) 948
BspT107I GGYRCC 1 cut(s) 913
BspTNI GGTCTC 1 cut(s) 1229
BsrI ACTGG 4 cut(s) 153, 1128, 1143, 1188
BssECI CCNNGG 5 cut(s) 415, 792, 1000, 1083, 1239
BssMI GATC 5 cut(s) 332, 672, 850, 862, 1210
BssT1I CCWWGG 3 cut(s) 415, 1083, 1239
Bst4CI ACNGT 2 cut(s) 314, 899
Bst6I CTCTTC 1 cut(s) 948
BstAPI GCANNNNNTGC 1 cut(s) 593
BstBAI YACGTR 2 cut(s) 428, 1023
BstC8I GCNNGC 3 cut(s) 504, 888, 973
BstDEI CTNAG 3 cut(s) 223, 240, 942
BstDSI CCRYGG 3 cut(s) 415, 1000, 1083
BstF5I GGATG 3 cut(s) 475, 913, 931
BstFNI CGCG 1 cut(s) 677
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 5 cut(s) 335, 675, 853, 865, 1213
BstMAI GTCTC 2 cut(s) 630, 1229
BstMBI GATC 5 cut(s) 332, 672, 850, 862, 1210
BstMCI CGRYCG 1 cut(s) 675
BstMWI GCNNNNNNNGC 2 cut(s) 593, 668
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 2 cut(s) 791, 792
BstSFI CTRYAG 1 cut(s) 313
BstUI CGCG 1 cut(s) 677
BstV1I GCAGC 5 cut(s) 21, 418, 580, 955, 977
BstV2I GAAGAC 2 cut(s) 79, 104
BstX2I RGATCY 2 cut(s) 332, 850
BstYI RGATCY 2 cut(s) 332, 850
BsuI GTATCC 2 cut(s) 254, 553
BsuRI GGCC 4 cut(s) 381, 414, 797, 872
BtgI CCRYGG 3 cut(s) 415, 1000, 1083
BtgZI GCGATG 1 cut(s) 723
BtsCI GGATG 3 cut(s) 475, 913, 931
BtsI GCAGTG 2 cut(s) 987, 1012
BtsIMutI CAGTG 3 cut(s) 160, 987, 1012
Cac8I GCNNGC 3 cut(s) 504, 888, 973
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 3 cut(s) 468, 865, 1045
CfoI GCGC 1 cut(s) 679
Cfr13I GGNCC 1 cut(s) 795
Cfr9I CCCGGG 1 cut(s) 792
Csp6I GTAC 3 cut(s) 310, 914, 1146
CspCI CAANNNNNGTGG 2 cut(s) 468, 503
CviAII CATG 8 cut(s) 394, 416, 469, 748, 762, 866, 1046, 1084
CviQI GTAC 3 cut(s) 310, 914, 1146
DdeI CTNAG 3 cut(s) 223, 240, 942
DpnI GATC 5 cut(s) 334, 674, 852, 864, 1212
DpnII GATC 5 cut(s) 332, 672, 850, 862, 1210
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
Eco130I CCWWGG 3 cut(s) 415, 1083, 1239
Eco24I GRGCYC 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 1229
Eco32I GATATC 1 cut(s) 358
Eco57I CTGAAG 3 cut(s) 464, 836, 979
Eco72I CACGTG 2 cut(s) 428, 1023
Eco88I CYCGRG 4 cut(s) 545, 640, 792, 839
EcoRV GATATC 1 cut(s) 358
EcoT14I CCWWGG 3 cut(s) 415, 1083, 1239
EcoT38I GRGCYC 1 cut(s) 960
ErhI CCWWGG 3 cut(s) 415, 1083, 1239
FaeI CATG 8 cut(s) 397, 419, 472, 751, 765, 869, 1049, 1087
FaqI GGGAC 1 cut(s) 775
FatI CATG 8 cut(s) 393, 415, 468, 747, 761, 865, 1045, 1083
FauI CCCGC 1 cut(s) 980
Fnu4HI GCNGC 6 cut(s) 10, 236, 432, 594, 966, 969
FokI GGATG 3 cut(s) 462, 920, 938
FriOI GRGCYC 1 cut(s) 960
Fsp4HI GCNGC 6 cut(s) 10, 236, 432, 594, 966, 969
FspBI CTAG 3 cut(s) 129, 174, 854
GlaI GCGC 1 cut(s) 678
GluI GCNGC 6 cut(s) 10, 236, 432, 594, 966, 969
GsaI CCCAGC 1 cut(s) 666
GsuI CTGGAG 1 cut(s) 225
HaeIII GGCC 4 cut(s) 381, 414, 797, 872
HapII CCGG 1 cut(s) 793
HhaI GCGC 1 cut(s) 679
Hin1II CATG 8 cut(s) 397, 419, 472, 751, 765, 869, 1049, 1087
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HindIII AAGCTT 2 cut(s) 295, 363
HinfI GANTC 7 cut(s) 548, 638, 1058, 1091, 1133, 1163, 1187
HpaI GTTAAC 1 cut(s) 981
HpaII CCGG 1 cut(s) 793
HphI GGTGA 1 cut(s) 1256
Hpy166II GTNNAC 2 cut(s) 425, 981
Hpy188I TCNGA 6 cut(s) 323, 553, 685, 945, 1096, 1192
Hpy188III TCNNGA 4 cut(s) 469, 854, 866, 1046
Hpy8I GTNNAC 2 cut(s) 425, 981
HpyAV CCTTC 3 cut(s) 371, 439, 1003
HpyCH4III ACNGT 2 cut(s) 314, 899
HpyCH4IV ACGT 3 cut(s) 214, 427, 1022
HpyCH4V TGCA 7 cut(s) 9, 393, 626, 890, 1010, 1026, 1151
HpyF10VI GCNNNNNNNGC 2 cut(s) 593, 668
HpyF3I CTNAG 3 cut(s) 223, 240, 942
HpySE526I ACGT 3 cut(s) 214, 427, 1022
Hsp92II CATG 8 cut(s) 397, 419, 472, 751, 765, 869, 1049, 1087
HspAI GCGC 1 cut(s) 677
KpnI GGTACC 1 cut(s) 917
KspAI GTTAAC 1 cut(s) 981
Kzo9I GATC 5 cut(s) 332, 672, 850, 862, 1210
LguI GCTCTTC 1 cut(s) 948
LmnI GCTCC 1 cut(s) 584
Lsp1109I GCAGC 5 cut(s) 21, 418, 580, 955, 977
LweI GCATC 4 cut(s) 484, 596, 916, 1189
MaeI CTAG 3 cut(s) 129, 174, 854
MaeII ACGT 3 cut(s) 214, 427, 1022
MaeIII GTNAC 2 cut(s) 803, 893
MalI GATC 5 cut(s) 334, 674, 852, 864, 1212
MboI GATC 5 cut(s) 332, 672, 850, 862, 1210
MboII GAAGA 5 cut(s) 84, 109, 193, 356, 965
MflI RGATCY 2 cut(s) 332, 850
MhlI GDGCHC 1 cut(s) 960
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 5 cut(s) 159, 482, 723, 821, 1037
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 2 cut(s) 557, 632
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 6 cut(s) 317, 554, 636, 862, 916, 943
Mox20I TGGCCA 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 414
MseI TTAA 5 cut(s) 93, 459, 509, 980, 1035
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 968
MspI CCGG 1 cut(s) 793
MspR9I CCNGG 2 cut(s) 793, 794
Mva1269I GAATGC 2 cut(s) 539, 816
MvnI CGCG 1 cut(s) 677
MwoI GCNNNNNNNGC 2 cut(s) 593, 668
NciI CCSGG 2 cut(s) 793, 794
NcoI CCATGG 2 cut(s) 415, 1083
NdeII GATC 5 cut(s) 332, 672, 850, 862, 1210
NlaIII CATG 8 cut(s) 397, 419, 472, 751, 765, 869, 1049, 1087
NlaIV GGNNCC 5 cut(s) 580, 611, 915, 1098, 1128
NmeAIII GCCGAG 1 cut(s) 898
NmuCI GTSAC 1 cut(s) 893
NspI RCATGY 1 cut(s) 397
PaeR7I CTCGAG 2 cut(s) 545, 640
PagI TCATGA 3 cut(s) 468, 865, 1045
PciSI GCTCTTC 1 cut(s) 948
PctI GAATGC 2 cut(s) 539, 816
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 5 cut(s) 1058, 1091, 1133, 1163, 1187
PkrI GCNGC 6 cut(s) 11, 237, 433, 595, 967, 970
Ple19I CGATCG 1 cut(s) 675
PleI GAGTC 2 cut(s) 556, 632
PmaCI CACGTG 2 cut(s) 428, 1023
PmlI CACGTG 2 cut(s) 428, 1023
PpsI GAGTC 2 cut(s) 556, 632
Ppu21I YACGTR 2 cut(s) 428, 1023
PspCI CACGTG 2 cut(s) 428, 1023
PspFI CCCAGC 1 cut(s) 662
PspN4I GGNNCC 5 cut(s) 580, 611, 915, 1098, 1128
PspPI GGNCC 1 cut(s) 795
PspXI VCTCGAGB 2 cut(s) 545, 640
PsrI GAACNNNNNNTAC 2 cut(s) 828, 860
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 2 cut(s) 332, 850
PvuI CGATCG 1 cut(s) 675
PvuII CAGCTG 1 cut(s) 968
RgaI GCGATCGC 1 cut(s) 675
RsaI GTAC 3 cut(s) 311, 915, 1147
RsaNI GTAC 3 cut(s) 310, 914, 1146
RseI CAYNNNNRTG 1 cut(s) 618
SapI GCTCTTC 1 cut(s) 948
SaqAI TTAA 5 cut(s) 93, 459, 509, 980, 1035
SatI GCNGC 6 cut(s) 10, 236, 432, 594, 966, 969
Sau3AI GATC 5 cut(s) 332, 672, 850, 862, 1210
Sau96I GGNCC 1 cut(s) 795
ScaI AGTACT 1 cut(s) 1147
SchI GAGTC 2 cut(s) 557, 632
ScrFI CCNGG 2 cut(s) 793, 794
SduI GDGCHC 1 cut(s) 960
SfaAI GCGATCGC 1 cut(s) 675
SfaNI GCATC 4 cut(s) 484, 596, 916, 1189
SfcI CTRYAG 1 cut(s) 313
Sfr274I CTCGAG 2 cut(s) 545, 640
SgfI GCGATCGC 1 cut(s) 675
SlaI CTCGAG 2 cut(s) 545, 640
SmaI CCCGGG 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 4 cut(s) 66, 298, 545, 640
SmoI CTYRAG 4 cut(s) 66, 298, 545, 640
SpeI ACTAGT 2 cut(s) 128, 173
Sse9I AATT 5 cut(s) 159, 482, 723, 821, 1037
SsiI CCGC 3 cut(s) 236, 929, 973
SspI AATATT 1 cut(s) 405
SspMI CTAG 3 cut(s) 129, 174, 854
StyD4I CCNGG 2 cut(s) 791, 792
StyI CCWWGG 3 cut(s) 415, 1083, 1239
TaaI ACNGT 2 cut(s) 314, 899
TaiI ACGT 3 cut(s) 217, 430, 1025
TaqI TCGA 3 cut(s) 546, 641, 1071
TasI AATT 5 cut(s) 159, 482, 723, 821, 1037
TatI WGTACW 1 cut(s) 1145
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 5 cut(s) 1058, 1091, 1133, 1163, 1187
Tru1I TTAA 5 cut(s) 93, 459, 509, 980, 1035
Tru9I TTAA 5 cut(s) 93, 459, 509, 980, 1035
TscAI CASTG 3 cut(s) 160, 994, 1012
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 5 cut(s) 9, 431, 593, 965, 968
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 6 cut(s) 7, 19, 77, 485, 528, 726
TspGWI ACGGA 1 cut(s) 1031
TspMI CCCGGG 1 cut(s) 792
TspRI CASTG 3 cut(s) 160, 994, 1012
XapI RAATTY 1 cut(s) 1037
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 3 cut(s) 1120, 1150, 1180
XhoI CTCGAG 2 cut(s) 545, 640
XmaI CCCGGG 1 cut(s) 792
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 3 cut(s) 129, 174, 854
ZrmI AGTACT 1 cut(s) 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.