MD01G1216600.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005995
Physical Location & Seq
Reverse (-)
30944388 .. 30948081
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1216600.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGATATGCAAAAGTTACATAAGGAGTGTGATTGTTCATGCTGGGCATATCGTAACGTCTACGAAGGCAAGGGTGCATTTCAACAATACTGATAGATTTTGCATGCTTGCCAATTCGCGGAACCGAGCATTTAGGGCGAGTTTAGCAAGGGTGCATTTCAACAATACTGATAGATTTTGCATGCTTGCCAATTCGCGGAACCGAGCATTTAGGGTGAGTTTAAGGATGATGGTTGATACAGGTGCGACGGATAACCAGGGATCCGTTTTTGATGTGCTGCCTGACAAAGATGAGGATGGTGAATATACCAGTGGAGGGTGTAAAAGCGAAGGTGGAAAACTGAGTTGCGGTTATTCAAGTTTCAGGGGAAAAAGAGCAACCATGGAGGATTTTTATGATGTTAAAATGTCCAAAATTGATGGGCAAATGGTCTGCCTGTTTGGAATTTTCGATGGTCACGGTGGTTCTCGTGCTGCTCAATACTTGAAGGAGCATCTATTTGACAATCTCATGAAGCATCCACAATTCATGACAGACACAAAGTTAGCTATTAGTGAATCGTATCAACAGACCGATGCAGACTTTTTGGATTCTGAAAGAGATACATACCGGGATGATGGTTCTACTGCTTCAACAGCAGTCTTGGTTGGTAACCATCTATATGTTGCCAATGTTGGAGATTCACGTACAGTAATATCAAAGGCAGGAAAAGCTATTGCTTTATCCGAGGATCATAAACCAAATCGAAGTGATGAGAGGAAGAGAATTGAAAATGCTGGGGGTGTTGTAATGTGGGCAGGCACTTGGAGGGTAGGAGGTGTGCTGGCTATGTCTCGGGCTTTTGGGAACCGCATGTTAAAGCAATATGTTGTTGCAGAACCTGAGATCCAGGACCAGGTGGTGGATGATGATTTTGAGTTTCTAGTGCTTGCTAGTGATGGGGTATGGGATGTGCTAACGAATGAGGTTGCTGTTGAAGTTGCCAAAACAGAAGAAGAACCCGAGGCAGCAGCTCGAAAGTTAACGGCGGTCGCCTTCTGCCGTGGCAGTGCCGACAACATAACGTGCATTGTGGTGAAGTTCCGGCATGACAAAGCAGAGCCAGCCAGCGCTCACCAAGATTAG

Protein Analysis

375

Amino Acids

41.48

Weight (kDa)

6.15

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 117 - 354 3.1e-63 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 901
AccI GTMKAC 1 cut(s) 59
AccII CGCG 2 cut(s) 118, 196
AciI CCGC 5 cut(s) 118, 196, 348, 850, 1028
AclWI GGATC 4 cut(s) 255, 268, 738, 880
AcsI RAATTY 1 cut(s) 444
AfaI GTAC 1 cut(s) 688
AfeI AGCGCT 1 cut(s) 1111
AfiI CCNNNNNNNGG 5 cut(s) 117, 195, 315, 895, 901
AgsI TTSAA 7 cut(s) 82, 160, 357, 487, 633, 770, 977
AjnI CCWGG 3 cut(s) 255, 888, 894
AloI GAACNNNNNNTCC 2 cut(s) 870, 902
AluBI AGCT 3 cut(s) 548, 713, 1013
AluI AGCT 3 cut(s) 548, 713, 1013
Alw26I GTCTC 1 cut(s) 837
AlwI GGATC 4 cut(s) 255, 268, 738, 880
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 2 cut(s) 834, 1001
Aor51HI AGCGCT 1 cut(s) 1111
ApeKI GCWGC 4 cut(s) 277, 473, 1007, 1010
ApoI RAATTY 1 cut(s) 444
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 1 cut(s) 892
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 4 cut(s) 226, 311, 1087, 1106
AvaI CYCGRG 2 cut(s) 834, 1001
AvaII GGWCC 1 cut(s) 892
BamHI GGATCC 1 cut(s) 260
BauI CACGAG 1 cut(s) 468
BbvI GCAGC 4 cut(s) 264, 460, 1019, 1022
BccI CCATC 7 cut(s) 223, 290, 413, 446, 611, 663, 932
BceAI ACGGC 2 cut(s) 1026, 1041
BciT130I CCWGG 3 cut(s) 257, 890, 896
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 1 cut(s) 837
BfaI CTAG 2 cut(s) 923, 933
BfoI RGCGCY 1 cut(s) 1113
BisI GCNGC 4 cut(s) 278, 474, 1008, 1011
BlsI GCNGC 4 cut(s) 279, 475, 1009, 1012
Bme1390I CCNGG 4 cut(s) 257, 611, 890, 896
Bme18I GGWCC 1 cut(s) 892
BmeT110I CYCGRG 2 cut(s) 834, 1001
BmgT120I GGNCC 1 cut(s) 892
BmiI GGNNCC 4 cut(s) 122, 200, 262, 848
BmrFI CCNGG 4 cut(s) 257, 611, 890, 896
BmsI GCATC 3 cut(s) 502, 526, 565
BpuMI CCSGG 1 cut(s) 611
BsaAI YACGTR 1 cut(s) 686
BsaJI CCNNGG 5 cut(s) 256, 381, 726, 1002, 1042
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 195, 315, 895, 901
Bse1I ACTGG 1 cut(s) 309
BseBI CCWGG 3 cut(s) 257, 890, 896
BseDI CCNNGG 5 cut(s) 256, 381, 726, 1002, 1042
BseGI GGATG 6 cut(s) 231, 301, 517, 619, 910, 955
BseLI CCNNNNNNNGG 5 cut(s) 117, 195, 315, 895, 901
BseMII CTCAG 2 cut(s) 332, 873
BseNI ACTGG 1 cut(s) 309
BseXI GCAGC 4 cut(s) 264, 460, 1019, 1022
BseYI CCCAGC 2 cut(s) 41, 776
Bsh1236I CGCG 2 cut(s) 118, 196
Bsh1285I CGRYCG 1 cut(s) 1032
BsiEI CGRYCG 1 cut(s) 1032
BsiHKCI CYCGRG 2 cut(s) 834, 1001
BsiSI CCGG 2 cut(s) 610, 1084
BslI CCNNNNNNNGG 5 cut(s) 117, 195, 315, 895, 901
BsmAI GTCTC 1 cut(s) 837
BsoBI CYCGRG 2 cut(s) 834, 1001
Bsp143I GATC 3 cut(s) 260, 730, 885
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 5 cut(s) 118, 196, 348, 850, 1028
BspCNI CTCAG 2 cut(s) 333, 874
BspFNI CGCG 2 cut(s) 118, 196
BspHI TCATGA 2 cut(s) 510, 528
BspLI GGNNCC 4 cut(s) 122, 200, 262, 848
BspPI GGATC 4 cut(s) 255, 268, 738, 880
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 5 cut(s) 256, 381, 726, 1002, 1042
BssMI GATC 3 cut(s) 260, 730, 885
BssSI CACGAG 1 cut(s) 468
BssT1I CCWWGG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 3 cut(s) 257, 890, 896
Bst4CI ACNGT 2 cut(s) 461, 691
Bst6I CTCTTC 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 9 cut(s) 104, 108, 182, 186, 799, 825, 930, 1104, 1108
BstDEI CTNAG 2 cut(s) 341, 882
BstDSI CCRYGG 2 cut(s) 381, 1042
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 6 cut(s) 231, 301, 517, 619, 910, 955
BstFNI CGCG 2 cut(s) 118, 196
BstH2I RGCGCY 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 1112
BstKTI GATC 3 cut(s) 263, 733, 888
BstMAI GTCTC 1 cut(s) 837
BstMBI GATC 3 cut(s) 260, 730, 885
BstMCI CGRYCG 1 cut(s) 1032
BstMWI GCNNNNNNNGC 5 cut(s) 134, 143, 635, 710, 1103
BstNI CCWGG 3 cut(s) 257, 890, 896
BstNSI RCATGY 3 cut(s) 106, 184, 856
BstPI GGTNACC 1 cut(s) 650
BstSCI CCNGG 4 cut(s) 255, 609, 888, 894
BstUI CGCG 2 cut(s) 118, 196
BstV1I GCAGC 4 cut(s) 264, 460, 1019, 1022
BstX2I RGATCY 2 cut(s) 260, 885
BstYI RGATCY 2 cut(s) 260, 885
BtgI CCRYGG 2 cut(s) 381, 1042
BtsCI GGATG 6 cut(s) 231, 301, 517, 619, 910, 955
BtsI GCAGTG 1 cut(s) 1054
BtsIMutI CAGTG 2 cut(s) 316, 1054
Cac8I GCNNGC 9 cut(s) 104, 108, 182, 186, 799, 825, 930, 1104, 1108
CaiI CAGNNNCTG 1 cut(s) 881
CciI TCATGA 2 cut(s) 510, 528
CfoI GCGC 1 cut(s) 1112
Cfr13I GGNCC 1 cut(s) 892
CsiI ACCWGGT 1 cut(s) 894
Csp6I GTAC 1 cut(s) 687
CviAII CATG 8 cut(s) 38, 103, 181, 382, 511, 529, 853, 1088
CviJI RGCY 7 cut(s) 548, 713, 827, 839, 1013, 1102, 1106
CviKI_1 RGCY 7 cut(s) 548, 713, 827, 839, 1013, 1102, 1106
CviQI GTAC 1 cut(s) 687
DdeI CTNAG 2 cut(s) 341, 882
DpnI GATC 3 cut(s) 262, 732, 887
DpnII GATC 3 cut(s) 260, 730, 885
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
Eco130I CCWWGG 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 892
Eco47III AGCGCT 1 cut(s) 1111
Eco88I CYCGRG 2 cut(s) 834, 1001
Eco91I GGTNACC 1 cut(s) 650
EcoO65I GGTNACC 1 cut(s) 650
EcoRII CCWGG 3 cut(s) 255, 888, 894
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 8 cut(s) 41, 106, 184, 385, 514, 532, 856, 1091
FatI CATG 8 cut(s) 37, 102, 180, 381, 510, 528, 852, 1087
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 4 cut(s) 278, 474, 1008, 1011
FokI GGATG 6 cut(s) 238, 308, 504, 626, 917, 962
Fsp4HI GCNGC 4 cut(s) 278, 474, 1008, 1011
FspBI CTAG 2 cut(s) 923, 933
GlaI GCGC 1 cut(s) 1111
GluI GCNGC 4 cut(s) 278, 474, 1008, 1011
GsaI CCCAGC 2 cut(s) 45, 780
HaeII RGCGCY 1 cut(s) 1113
HapII CCGG 2 cut(s) 610, 1084
HhaI GCGC 1 cut(s) 1112
Hin1II CATG 8 cut(s) 41, 106, 184, 385, 514, 532, 856, 1091
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 3 cut(s) 557, 590, 680
HpaI GTTAAC 1 cut(s) 1023
HpaII CCGG 2 cut(s) 610, 1084
HphI GGTGA 4 cut(s) 226, 311, 1087, 1106
Hpy166II GTNNAC 2 cut(s) 60, 1023
Hpy188I TCNGA 2 cut(s) 595, 727
Hpy188III TCNNGA 2 cut(s) 511, 529
Hpy8I GTNNAC 2 cut(s) 60, 1023
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 4 cut(s) 58, 323, 481, 1045
HpyCH4III ACNGT 2 cut(s) 461, 691
HpyCH4IV ACGT 3 cut(s) 56, 685, 1064
HpyCH4V TGCA 8 cut(s) 9, 76, 102, 154, 180, 578, 875, 1068
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 143, 635, 710, 1103
HpyF3I CTNAG 2 cut(s) 341, 882
HpySE526I ACGT 3 cut(s) 56, 685, 1064
Hsp92II CATG 8 cut(s) 41, 106, 184, 385, 514, 532, 856, 1091
HspAI GCGC 1 cut(s) 1110
KspAI GTTAAC 1 cut(s) 1023
Kzo9I GATC 3 cut(s) 260, 730, 885
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 4 cut(s) 264, 460, 1019, 1022
LweI GCATC 3 cut(s) 502, 526, 565
MabI ACCWGGT 1 cut(s) 894
MaeI CTAG 2 cut(s) 923, 933
MaeII ACGT 3 cut(s) 56, 685, 1064
MaeIII GTNAC 4 cut(s) 14, 52, 455, 650
MalI GATC 3 cut(s) 262, 732, 887
MboI GATC 3 cut(s) 260, 730, 885
MboII GAAGA 3 cut(s) 772, 1004, 1007
MflI RGATCY 2 cut(s) 260, 885
MluCI AATT 6 cut(s) 112, 190, 414, 444, 524, 765
MmeI TCCRAC 1 cut(s) 655
MnlI CCTC 9 cut(s) 286, 308, 379, 721, 750, 801, 809, 958, 997
MseI TTAA 4 cut(s) 221, 402, 857, 1022
MslI CAYNNNNRTG 2 cut(s) 660, 1073
MspI CCGG 2 cut(s) 610, 1084
MspR9I CCNGG 4 cut(s) 257, 611, 890, 896
MvaI CCWGG 3 cut(s) 257, 890, 896
MvnI CGCG 2 cut(s) 118, 196
MwoI GCNNNNNNNGC 5 cut(s) 134, 143, 635, 710, 1103
NciI CCSGG 1 cut(s) 611
NcoI CCATGG 1 cut(s) 381
NdeII GATC 3 cut(s) 260, 730, 885
NlaIII CATG 8 cut(s) 41, 106, 184, 385, 514, 532, 856, 1091
NlaIV GGNNCC 4 cut(s) 122, 200, 262, 848
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 3 cut(s) 106, 184, 856
PaeI GCATGC 2 cut(s) 106, 184
PagI TCATGA 2 cut(s) 510, 528
PfeI GAWTC 3 cut(s) 557, 590, 680
PflMI CCANNNNNTGG 1 cut(s) 901
PfoI TCCNGGA 1 cut(s) 888
PkrI GCNGC 4 cut(s) 279, 475, 1009, 1012
Ppu21I YACGTR 1 cut(s) 686
Psp6I CCWGG 3 cut(s) 255, 888, 894
PspEI GGTNACC 1 cut(s) 650
PspFI CCCAGC 2 cut(s) 41, 776
PspGI CCWGG 3 cut(s) 255, 888, 894
PspN4I GGNNCC 4 cut(s) 122, 200, 262, 848
PspPI GGNCC 1 cut(s) 892
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 2 cut(s) 260, 885
RsaI GTAC 1 cut(s) 688
RsaNI GTAC 1 cut(s) 687
RseI CAYNNNNRTG 2 cut(s) 660, 1073
SaqAI TTAA 4 cut(s) 221, 402, 857, 1022
SatI GCNGC 4 cut(s) 278, 474, 1008, 1011
Sau3AI GATC 3 cut(s) 260, 730, 885
Sau96I GGNCC 1 cut(s) 892
ScrFI CCNGG 4 cut(s) 257, 611, 890, 896
SexAI ACCWGGT 1 cut(s) 894
SfaNI GCATC 3 cut(s) 502, 526, 565
SinI GGWCC 1 cut(s) 892
SmiMI CAYNNNNRTG 2 cut(s) 660, 1073
SphI GCATGC 2 cut(s) 106, 184
Sse9I AATT 6 cut(s) 112, 190, 414, 444, 524, 765
SsiI CCGC 5 cut(s) 118, 196, 348, 850, 1028
SspMI CTAG 2 cut(s) 923, 933
StyD4I CCNGG 4 cut(s) 255, 609, 888, 894
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 461, 691
TaiI ACGT 3 cut(s) 59, 688, 1067
TaqI TCGA 3 cut(s) 450, 745, 1015
TaqII GACCGA 1 cut(s) 587
TasI AATT 6 cut(s) 112, 190, 414, 444, 524, 765
TfiI GAWTC 3 cut(s) 557, 590, 680
Tru1I TTAA 4 cut(s) 221, 402, 857, 1022
Tru9I TTAA 4 cut(s) 221, 402, 857, 1022
TscAI CASTG 2 cut(s) 316, 1054
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 4 cut(s) 277, 473, 1007, 1010
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 3 cut(s) 26, 517, 527
TspGWI ACGGA 2 cut(s) 253, 263
TspRI CASTG 2 cut(s) 316, 1054
Van91I CCANNNNNTGG 1 cut(s) 901
VpaK11BI GGWCC 1 cut(s) 892
XapI RAATTY 1 cut(s) 444
XceI RCATGY 3 cut(s) 106, 184, 856
XmiI GTMKAC 1 cut(s) 59
XspI CTAG 2 cut(s) 923, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.