Rroxscaffold_4G00279540

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005995
Physical Location & Seq
Forward (+)
2318199 .. 2322643
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279540.1

Sequence Viewer

Length: 1050 bp
ATGATATGCAAGAGTGGGATAAGGAGTTTGATTGTTCAAGCTGGGAATATAGTAGCGTGCACAAAAACTGGGGCGTATTTGAACAACATTGGGAGAGTTTGCATGCAAACCTCTTTGGGAAACCGTGAATTTAAGACAACGAGTTTAAGGATGATGGTTGATTCTAGTGCGACCGAGAAACAGGGTTCCATTGTTGATGTCTTATTGCAGAAAGATGACGAGGGTGGATATATCAGTGGAGGCTGGAAAAGTGAAGGCGGAAAACTTAGCTGTGGCTATTCCAGTTTTAGGGGAAAAAGAGCCACCATGGAGGATTTTTATGATATAAAAACTTCCAAGATTGATGGGCAAACAGTTTGCCTCTTTGGAATTTTTGATGGTCATGGTGGTTCTCGTGCTGCTGAATATCTGAAGGAGCATCTATTTGAGAATCTCATGAAGCATCCAAACTTTATCACAGACACCAAGTTGGCTATTAGTGAATCATATCAACAAACTGATGCAGCTTTTTTGGATTCTGAAAGGGATACATACCGTGATGATGGTTCTACTGCTTCAACAGCAGTCTTAGTGGGTAACCATCTATACGTTGCCAATGTTGGAGATTCTCGTACAGTAATATCAAAAGCAGGCAAAGCAATTCCTCTATCTGAGGATCATAAACCAAATCGAACTGATGAGAGAAAGAGAATTGAAAATGCTGGGGGCATTGTAATGTGGGCAGGAACTTGGAGGGTAGGGGGTGTTTTGGCTATGTCTCGGGCATTTGGAAACCGCATGTTGAAGCAATTTGTTGTTGCAGAACCTGAAATCCAGGACCAAGTGATAGACGAAGAATTTGAATTGCTGGTGCTCGCTAGTGATGGGCTGTGGGATGTGGTGCCAAATGAGGATGCTGTTTCACTTGCCCGAACAGAAGAAGAACCTGAGGCGGCAGCTCGGAAGTTAACGGGAACAGCATTTGCCCGTGGTAGTGCAGACAATATAACATGCATTGTGGTGAGGTTCCACCATGACAAAGCAGAGTCTGCTGGGGCTAACCATGATTAG

Protein Analysis

349

Amino Acids

38.09

Weight (kDa)

5.49

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 91 - 329 4.5e-64 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 880
AciI CCGC 3 cut(s) 258, 775, 932
AclWI GGATC 1 cut(s) 663
AcsI RAATTY 3 cut(s) 128, 369, 836
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 1 cut(s) 613
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 6 cut(s) 38, 82, 558, 695, 784, 842
AjnI CCWGG 1 cut(s) 813
AloI GAACNNNNNNTCC 2 cut(s) 795, 827
AluBI AGCT 4 cut(s) 41, 270, 506, 938
AluI AGCT 4 cut(s) 41, 270, 506, 938
Alw21I GWGCWC 2 cut(s) 62, 855
Alw26I GTCTC 1 cut(s) 762
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 1 cut(s) 663
AlwNI CAGNNNCTG 2 cut(s) 806, 1028
Ama87I CYCGRG 1 cut(s) 759
ApaLI GTGCAC 1 cut(s) 58
ApeKI GCWGC 3 cut(s) 398, 503, 935
ApoI RAATTY 3 cut(s) 128, 369, 836
ArsI GACNNNNNNTTYG 2 cut(s) 821, 853
AspS9I GGNCC 1 cut(s) 817
AsuHPI GGTGA 1 cut(s) 1012
AvaI CYCGRG 1 cut(s) 759
AvaII GGWCC 1 cut(s) 817
AxyI CCTNAGG 1 cut(s) 927
BaeGI GKGCMC 1 cut(s) 62
BanI GGYRCC 1 cut(s) 880
BauI CACGAG 1 cut(s) 393
Bbv12I GWGCWC 2 cut(s) 62, 855
BbvI GCAGC 3 cut(s) 385, 515, 947
BccI CCATC 6 cut(s) 148, 338, 371, 536, 588, 857
BciT130I CCWGG 1 cut(s) 815
BciVI GTATCC 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 762
BfaI CTAG 2 cut(s) 165, 858
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 4 cut(s) 399, 504, 933, 936
BlsI GCNGC 4 cut(s) 400, 505, 934, 937
Bme1390I CCNGG 1 cut(s) 815
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 759
BmgT120I GGNCC 1 cut(s) 817
BmiI GGNNCC 3 cut(s) 187, 882, 1007
BmrFI CCNGG 1 cut(s) 815
BmrI ACTGGG 1 cut(s) 78
BmsI GCATC 4 cut(s) 427, 451, 490, 883
BmuI ACTGGG 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 306, 967
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 2 cut(s) 73, 282
Bse21I CCTNAGG 1 cut(s) 927
BseBI CCWGG 1 cut(s) 815
BseDI CCNNGG 2 cut(s) 306, 967
BseGI GGATG 4 cut(s) 156, 442, 880, 898
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMII CTCAG 2 cut(s) 642, 918
BseNI ACTGG 2 cut(s) 73, 282
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 3 cut(s) 385, 515, 947
BseYI CCCAGC 3 cut(s) 41, 701, 1031
BsgI GTGCAG 1 cut(s) 996
Bsh1285I CGRYCG 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 880
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 62, 855
BsiHKCI CYCGRG 1 cut(s) 759
BslI CCNNNNNNNGG 1 cut(s) 288
BsmAI GTCTC 1 cut(s) 762
BsoBI CYCGRG 1 cut(s) 759
Bsp1286I GDGCHC 2 cut(s) 62, 855
Bsp143I GATC 1 cut(s) 655
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 3 cut(s) 258, 775, 932
BspCNI CTCAG 2 cut(s) 643, 919
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 187, 882, 1007
BspPI GGATC 1 cut(s) 663
BspT107I GGYRCC 1 cut(s) 880
BsrI ACTGG 2 cut(s) 73, 282
BssECI CCNNGG 2 cut(s) 306, 967
BssMI GATC 1 cut(s) 655
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 4 cut(s) 125, 355, 536, 616
BstAPI GCANNNNNTGC 1 cut(s) 1028
BstC8I GCNNGC 4 cut(s) 58, 104, 631, 855
BstDEI CTNAG 4 cut(s) 266, 568, 651, 927
BstDSI CCRYGG 2 cut(s) 306, 967
BstEII GGTNACC 1 cut(s) 575
BstF5I GGATG 4 cut(s) 156, 442, 880, 898
BstKTI GATC 1 cut(s) 658
BstMAI GTCTC 1 cut(s) 762
BstMBI GATC 1 cut(s) 655
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 3 cut(s) 560, 635, 1028
BstNI CCWGG 1 cut(s) 815
BstNSI RCATGY 3 cut(s) 106, 781, 993
BstPI GGTNACC 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 62
BstV1I GCAGC 3 cut(s) 385, 515, 947
Bsu36I CCTNAGG 1 cut(s) 927
BsuI GTATCC 1 cut(s) 520
BtgI CCRYGG 2 cut(s) 306, 967
BtsCI GGATG 4 cut(s) 156, 442, 880, 898
BtsIMutI CAGTG 1 cut(s) 241
Cac8I GCNNGC 4 cut(s) 58, 104, 631, 855
CaiI CAGNNNCTG 2 cut(s) 806, 1028
CciI TCATGA 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 817
Csp6I GTAC 1 cut(s) 612
CviAII CATG 8 cut(s) 103, 307, 383, 436, 778, 990, 1013, 1043
CviQI GTAC 1 cut(s) 612
DdeI CTNAG 4 cut(s) 266, 568, 651, 927
DpnI GATC 1 cut(s) 657
DpnII GATC 1 cut(s) 655
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 817
Eco57I CTGAAG 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 927
Eco88I CYCGRG 1 cut(s) 759
Eco91I GGTNACC 1 cut(s) 575
EcoO65I GGTNACC 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 813
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 995
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 8 cut(s) 106, 310, 386, 439, 781, 993, 1016, 1046
FatI CATG 8 cut(s) 102, 306, 382, 435, 777, 989, 1012, 1042
Fnu4HI GCNGC 4 cut(s) 399, 504, 933, 936
FokI GGATG 4 cut(s) 163, 429, 887, 905
Fsp4HI GCNGC 4 cut(s) 399, 504, 933, 936
FspBI CTAG 2 cut(s) 165, 858
GluI GCNGC 4 cut(s) 399, 504, 933, 936
GsaI CCCAGC 3 cut(s) 45, 705, 1035
Hin1II CATG 8 cut(s) 106, 310, 386, 439, 781, 993, 1016, 1046
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HinfI GANTC 6 cut(s) 161, 430, 482, 515, 605, 1025
HpaI GTTAAC 1 cut(s) 948
HphI GGTGA 1 cut(s) 1012
Hpy166II GTNNAC 2 cut(s) 60, 948
Hpy188I TCNGA 4 cut(s) 411, 520, 652, 942
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 2 cut(s) 60, 948
HpyAV CCTTC 2 cut(s) 248, 406
HpyCH4III ACNGT 4 cut(s) 125, 355, 536, 616
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 9 cut(s) 9, 60, 102, 106, 208, 503, 800, 977, 993
HpyF10VI GCNNNNNNNGC 3 cut(s) 560, 635, 1028
HpyF3I CTNAG 4 cut(s) 266, 568, 651, 927
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 8 cut(s) 106, 310, 386, 439, 781, 993, 1016, 1046
KspAI GTTAAC 1 cut(s) 948
Kzo9I GATC 1 cut(s) 655
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 3 cut(s) 385, 515, 947
LweI GCATC 4 cut(s) 427, 451, 490, 883
MaeI CTAG 2 cut(s) 165, 858
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 1 cut(s) 575
MalI GATC 1 cut(s) 657
MboI GATC 1 cut(s) 655
MboII GAAGA 3 cut(s) 845, 929, 932
MhlI GDGCHC 2 cut(s) 62, 855
MluCI AATT 7 cut(s) 128, 369, 639, 690, 788, 836, 842
MlyI GAGTC 1 cut(s) 1034
MmeI TCCRAC 1 cut(s) 580
Mph1103I ATGCAT 1 cut(s) 995
MseI TTAA 3 cut(s) 132, 146, 947
MslI CAYNNNNRTG 2 cut(s) 713, 998
MspR9I CCNGG 1 cut(s) 815
MvaI CCWGG 1 cut(s) 815
MwoI GCNNNNNNNGC 3 cut(s) 560, 635, 1028
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 655
NlaIII CATG 8 cut(s) 106, 310, 386, 439, 781, 993, 1016, 1046
NlaIV GGNNCC 3 cut(s) 187, 882, 1007
NsiI ATGCAT 1 cut(s) 995
NspI RCATGY 3 cut(s) 106, 781, 993
PaeI GCATGC 1 cut(s) 106
PagI TCATGA 1 cut(s) 435
PfeI GAWTC 5 cut(s) 161, 430, 482, 515, 605
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 4 cut(s) 400, 505, 934, 937
PleI GAGTC 1 cut(s) 1033
PpsI GAGTC 1 cut(s) 1033
Psp6I CCWGG 1 cut(s) 813
PspEI GGTNACC 1 cut(s) 575
PspFI CCCAGC 3 cut(s) 41, 701, 1031
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 3 cut(s) 187, 882, 1007
PspPI GGNCC 1 cut(s) 817
PstNI CAGNNNCTG 2 cut(s) 806, 1028
RsaI GTAC 1 cut(s) 613
RsaNI GTAC 1 cut(s) 612
RseI CAYNNNNRTG 2 cut(s) 713, 998
SaqAI TTAA 3 cut(s) 132, 146, 947
SatI GCNGC 4 cut(s) 399, 504, 933, 936
Sau3AI GATC 1 cut(s) 655
Sau96I GGNCC 1 cut(s) 817
SchI GAGTC 1 cut(s) 1034
ScrFI CCNGG 1 cut(s) 815
SduI GDGCHC 2 cut(s) 62, 855
SetI ASST 9 cut(s) 43, 113, 272, 508, 591, 808, 928, 940, 1007
SfaNI GCATC 4 cut(s) 427, 451, 490, 883
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 2 cut(s) 713, 998
SphI GCATGC 1 cut(s) 106
Sse9I AATT 7 cut(s) 128, 369, 639, 690, 788, 836, 842
SsiI CCGC 3 cut(s) 258, 775, 932
SspMI CTAG 2 cut(s) 165, 858
StyD4I CCNGG 1 cut(s) 813
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 4 cut(s) 125, 355, 536, 616
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 670
TaqII GACCGA 1 cut(s) 188
TasI AATT 7 cut(s) 128, 369, 639, 690, 788, 836, 842
TauI GCSGC 1 cut(s) 935
TfiI GAWTC 5 cut(s) 161, 430, 482, 515, 605
Tru1I TTAA 3 cut(s) 132, 146, 947
Tru9I TTAA 3 cut(s) 132, 146, 947
TscAI CASTG 1 cut(s) 241
TseI GCWGC 3 cut(s) 398, 503, 935
TspDTI ATGAA 1 cut(s) 452
TspRI CASTG 1 cut(s) 241
VneI GTGCAC 1 cut(s) 58
VpaK11BI GGWCC 1 cut(s) 817
XapI RAATTY 3 cut(s) 128, 369, 836
XceI RCATGY 3 cut(s) 106, 781, 993
XspI CTAG 2 cut(s) 165, 858
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.