AT5G53540

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010923
Physical Location & Seq
Reverse (-)
21749279 .. 21751338
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53540.1

Sequence Viewer

Length: 1212 bp
ATGAAGAGATCTTCAGGGAGAAAGGATTCAAATTTCATAGAACAATTGATACTTTACGTCGCTAGTGCTGCATTGAGTAGCTTGGTTCTCTACGTTGGTCTCCGTCAACTTGACCCGAACCGTGACGCCGCTAAAAAGTCTCTCGAGCACAAAAGAGAAATCGCCAAACGTTTGGGTCGTCCTCTTATTCAAACCAATCAATACGAGGATGTGATTGCGTGTGATGTAATAAACCCATTACACATAGATGTTGAATTTGGTTCTATTGGAGGATTGGAGTCAATTAAGCAAGCTTTGTATGAGCTTGTGATTCTACCATTGAAAAGACCTGAGCTTTTTGCTTATGGGAAGTTACTGGGTCCTCAAAAGGGTGTTTTGTTGTATGGTCCTCCTGGAACTGGGAAGACTATGCTTGCTAAAGCGATTGCGAGAGAGTCTGAAGCTGTTTTTATTAATGTAAAGGTTTCTAATTTGATGAGCAAGTGGTTTGGTGATGCACAGAAGCTTGTGTCTGCTGTGTTTAGCTTGGCGTATAAACTCCAACCTGCTATTATTTTTATCGATGAGGTGGATAGCTTTCTTGGCCAGCGACGGTCGACTGATAATGAAGCAATGTCGAATATGAAGACCGAGTTTATGGCTTTATGGGATGGATTTACTACAGACCAGAATGCGAGGGTGATGGTTCTTGCTGCAACTAACAGACCATCAGAGCTCGATGAAGCGATATTGAGGCGTTTTCCTCAGTCATTTGAGATCGGGATGCCTGATTGCCAGGAGAGAGCGCAAATATTGAAAGTTGTTTTGAAAGGAGAGAGTGTTGAATCAGATATTAACTATGATCGTATAGCTCGTTTATGTGAGGACTATACCGGATCAGATATTTTTGAACTCTGCAAAAAAGCAGCTTACTTCCCAATCCGAGAAATCCTAGAGGCAGAGAAAGAGGGCAAACGAGTTTCTGTTCCTAGACCATTGACACAACTGGACTTGGAGAAGGTTCTTGCTACCTCAAAGAAGACACAAGTTGCTGCAAGCGAGTACACTGGTTTAAGCTCTCAATCTTCTGTCTGGGGAAGTCCAAGCAATGCCGATGAGGTCCAAGCAGCTATAAACGGCATCTCCAAGCTTTTTGTCTCGCAGCTTAGAAACATTCAGCCAGATTCTCAGGATTCATATCAACAACATCCTTCAGAAGGAGATTCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

403

Amino Acids

44.85

Weight (kDa)

5.72

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_5 PF07728 124 - 247 9e-08 AAA domain (dynein-related subfamily)
AAA PF00004 125 - 254 1.4e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 277 - 313 2.4e-10 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27680 AT5G53540
fragaria_vesca FvH4_7g32510
malus_domestica MD01G1225800.v1.1 MD07G1296300.v1.1
prunus_persica Prupe.2G315900_v2.0.a1
pyrus_communis pycom01g23430 pycom07g26910
rosa_chinensis RchiOBHm_Chr1g0382351
rosa_laevigata RLG00000026184
rosa_multiflora Rmu_sc0000110.1_g000041 Rmu_sc0006177.1_g000002 Rmu_sc0028160.1_g000002
rosa_roxburghii Rroxscaffold_4G00278170
rosa_rugosa Rorug01G0433600
rosa_samantha Rh1AG458300 Rh1BG414600 Rh1CG428700 Rh1DG446700
rosa_wichuraiana Rw1G041270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 553
AccI GTMKAC 1 cut(s) 596
AciI CCGC 1 cut(s) 129
AclI AACGTT 1 cut(s) 169
AclWI GGATC 1 cut(s) 883
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 2 cut(s) 31, 254
AcuI CTGAAG 2 cut(s) 459, 1176
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 1043
AfiI CCNNNNNNNGG 3 cut(s) 368, 398, 1196
AgsI TTSAA 8 cut(s) 30, 191, 254, 322, 796, 808, 824, 890
AjnI CCWGG 2 cut(s) 391, 774
Alw21I GWGCWC 2 cut(s) 150, 717
Alw26I GTCTC 3 cut(s) 104, 144, 1141
AlwI GGATC 1 cut(s) 883
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 1 cut(s) 583
ApeKI GCWGC 6 cut(s) 68, 692, 905, 1031, 1106, 1141
ApoI RAATTY 2 cut(s) 31, 254
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 1 cut(s) 787
AspS9I GGNCC 3 cut(s) 359, 386, 1099
AsuHPI GGTGA 2 cut(s) 503, 691
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 3 cut(s) 359, 386, 1099
BalI TGGCCA 1 cut(s) 585
BanII GRGCYC 1 cut(s) 717
BbsI GAAGAC 3 cut(s) 410, 632, 1025
Bbv12I GWGCWC 2 cut(s) 150, 717
BbvI GCAGC 6 cut(s) 55, 679, 917, 1018, 1118, 1153
BccI CCATC 3 cut(s) 644, 676, 715
BceAI ACGGC 1 cut(s) 1132
BciT130I CCWGG 2 cut(s) 393, 776
BcoDI GTCTC 3 cut(s) 104, 144, 1141
BfaI CTAG 3 cut(s) 63, 932, 969
BfmI CTRYAG 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 553
BglII AGATCT 1 cut(s) 8
BisI GCNGC 7 cut(s) 69, 129, 693, 906, 1032, 1107, 1142
BlsI GCNGC 7 cut(s) 70, 130, 694, 907, 1033, 1108, 1143
Bme1390I CCNGG 2 cut(s) 393, 776
Bme18I GGWCC 3 cut(s) 359, 386, 1099
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 359, 386, 1099
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 2 cut(s) 393, 776
BmrI ACTGGG 2 cut(s) 365, 408
BmsI GCATC 3 cut(s) 484, 753, 1128
BmuI ACTGGG 2 cut(s) 365, 408
BpiI GAAGAC 3 cut(s) 410, 632, 1025
Bpu10I CCTNAGC 1 cut(s) 330
Bsa29I ATCGAT 1 cut(s) 561
BsaBI GATNNNNATC 1 cut(s) 1176
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 104
BsaWI WCCGGW 1 cut(s) 872
BsaXI ACNNNNNCTCC 4 cut(s) 804, 834, 1106, 1136
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 398, 1196
Bse1I ACTGG 4 cut(s) 360, 403, 990, 1051
Bse3DI GCAATG 2 cut(s) 618, 1093
Bse8I GATNNNNATC 1 cut(s) 1176
BseBI CCWGG 2 cut(s) 393, 776
BseCI ATCGAT 1 cut(s) 561
BseGI GGATG 4 cut(s) 214, 655, 768, 1186
BseJI GATNNNNATC 1 cut(s) 1176
BseLI CCNNNNNNNGG 3 cut(s) 368, 398, 1196
BseMI GCAATG 2 cut(s) 618, 1093
BseMII CTCAG 3 cut(s) 321, 758, 1181
BseNI ACTGG 4 cut(s) 360, 403, 990, 1051
BseXI GCAGC 6 cut(s) 55, 679, 917, 1018, 1118, 1153
Bsh1285I CGRYCG 1 cut(s) 596
BshFI GGCC 1 cut(s) 585
BshVI ATCGAT 1 cut(s) 561
BsiEI CGRYCG 1 cut(s) 596
BsiHKAI GWGCWC 2 cut(s) 150, 717
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 873
BslI CCNNNNNNNGG 3 cut(s) 368, 398, 1196
BsmAI GTCTC 3 cut(s) 104, 144, 1141
BsmI GAATGC 1 cut(s) 676
BsnI GGCC 1 cut(s) 585
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 150, 717
Bsp143I GATC 4 cut(s) 8, 756, 841, 875
BspACI CCGC 1 cut(s) 129
BspANI GGCC 1 cut(s) 585
BspCNI CTCAG 3 cut(s) 322, 757, 1180
BspDI ATCGAT 1 cut(s) 561
BspLI GGNNCC 1 cut(s) 360
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 1 cut(s) 883
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 2 cut(s) 618, 1093
BsrI ACTGG 4 cut(s) 360, 403, 990, 1051
BssMI GATC 4 cut(s) 8, 756, 841, 875
BssNI GRCGYC 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 393, 776
Bst4CI ACNGT 2 cut(s) 122, 594
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 4 cut(s) 291, 414, 587, 1036
BstDEI CTNAG 4 cut(s) 330, 744, 1145, 1167
BstENI CCTNNNNNAGG 1 cut(s) 1194
BstF5I GGATG 4 cut(s) 214, 655, 768, 1186
BstHHI GCGC 1 cut(s) 787
BstKTI GATC 4 cut(s) 11, 759, 844, 878
BstMAI GTCTC 3 cut(s) 104, 144, 1141
BstMBI GATC 4 cut(s) 8, 756, 841, 875
BstMCI CGRYCG 1 cut(s) 596
BstMWI GCNNNNNNNGC 2 cut(s) 68, 582
BstNI CCWGG 2 cut(s) 393, 776
BstSCI CCNGG 2 cut(s) 391, 774
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 6 cut(s) 55, 679, 917, 1018, 1118, 1153
BstV2I GAAGAC 3 cut(s) 410, 632, 1025
BstX2I RGATCY 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 172
BstYI RGATCY 1 cut(s) 8
Bsu15I ATCGAT 1 cut(s) 561
BsuRI GGCC 1 cut(s) 585
BsuTUI ATCGAT 1 cut(s) 561
BtsCI GGATG 4 cut(s) 214, 655, 768, 1186
BtsIMutI CAGTG 1 cut(s) 1044
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 4 cut(s) 291, 414, 587, 1036
CfoI GCGC 1 cut(s) 787
Cfr13I GGNCC 3 cut(s) 359, 386, 1099
ClaI ATCGAT 1 cut(s) 561
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 1042
CviQI GTAC 1 cut(s) 1042
DdeI CTNAG 4 cut(s) 330, 744, 1145, 1167
DpnI GATC 4 cut(s) 10, 758, 843, 877
DpnII GATC 4 cut(s) 8, 756, 841, 875
EaeI YGGCCR 1 cut(s) 583
Ecl136II GAGCTC 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 717
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 3 cut(s) 359, 386, 1099
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 2 cut(s) 459, 1176
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 1194
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 391, 774
EcoT38I GRGCYC 1 cut(s) 717
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 7 cut(s) 69, 129, 693, 906, 1032, 1107, 1142
FokI GGATG 4 cut(s) 221, 662, 775, 1173
FriOI GRGCYC 1 cut(s) 717
Fsp4HI GCNGC 7 cut(s) 69, 129, 693, 906, 1032, 1107, 1142
FspBI CTAG 3 cut(s) 63, 932, 969
GlaI GCGC 1 cut(s) 786
GluI GCNGC 7 cut(s) 69, 129, 693, 906, 1032, 1107, 1142
HaeIII GGCC 1 cut(s) 585
HapII CCGG 1 cut(s) 873
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 787
Hin1I GRCGYC 1 cut(s) 126
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HincII GTYRAC 2 cut(s) 107, 597
HindII GTYRAC 2 cut(s) 107, 597
HindIII AAGCTT 3 cut(s) 291, 503, 1127
HinfI GANTC 8 cut(s) 26, 278, 310, 434, 824, 1163, 1172, 1202
HpaII CCGG 1 cut(s) 873
HphI GGTGA 2 cut(s) 503, 691
Hpy166II GTNNAC 3 cut(s) 107, 597, 1044
Hpy188I TCNGA 7 cut(s) 439, 712, 829, 880, 923, 1195, 1207
Hpy188III TCNNGA 3 cut(s) 143, 760, 1169
Hpy8I GTNNAC 3 cut(s) 107, 597, 1044
Hpy99I CGWCG 2 cut(s) 62, 594
HpyAV CCTTC 3 cut(s) 991, 1190, 1200
HpyCH4III ACNGT 2 cut(s) 122, 594
HpyCH4IV ACGT 3 cut(s) 57, 93, 169
HpyCH4V TGCA 5 cut(s) 71, 497, 695, 897, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 582
HpyF3I CTNAG 4 cut(s) 330, 744, 1145, 1167
HpySE526I ACGT 3 cut(s) 57, 93, 169
Hsp92I GRCGYC 1 cut(s) 126
HspAI GCGC 1 cut(s) 785
Kzo9I GATC 4 cut(s) 8, 756, 841, 875
Lsp1109I GCAGC 6 cut(s) 55, 679, 917, 1018, 1118, 1153
LweI GCATC 3 cut(s) 484, 753, 1128
MaeI CTAG 3 cut(s) 63, 932, 969
MaeII ACGT 3 cut(s) 57, 93, 169
MaeIII GTNAC 2 cut(s) 122, 351
MalI GATC 4 cut(s) 10, 758, 843, 877
MboI GATC 4 cut(s) 8, 756, 841, 875
MboII GAAGA 6 cut(s) 3, 16, 415, 637, 1030, 1056
MfeI CAATTG 1 cut(s) 44
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 2 cut(s) 150, 717
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 5 cut(s) 31, 44, 254, 282, 469
MluNI TGGCCA 1 cut(s) 585
MlyI GAGTC 2 cut(s) 287, 443
MmeI TCCRAC 1 cut(s) 565
Mox20I TGGCCA 1 cut(s) 585
MroXI GAANNNNTTC 1 cut(s) 25
MscI TGGCCA 1 cut(s) 585
MseI TTAA 4 cut(s) 285, 453, 834, 1052
MslI CAYNNNNRTG 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 585
MspI CCGG 1 cut(s) 873
MspR9I CCNGG 2 cut(s) 393, 776
MunI CAATTG 1 cut(s) 44
Mva1269I GAATGC 1 cut(s) 676
MvaI CCWGG 2 cut(s) 393, 776
MwoI GCNNNNNNNGC 2 cut(s) 68, 582
NdeII GATC 4 cut(s) 8, 756, 841, 875
NlaIV GGNNCC 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 143
PcsI WCGNNNNNNNCGW 2 cut(s) 175, 850
PctI GAATGC 1 cut(s) 676
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 6 cut(s) 26, 310, 824, 1163, 1172, 1202
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 7 cut(s) 70, 130, 694, 907, 1033, 1108, 1143
PleI GAGTC 2 cut(s) 286, 442
PpsI GAGTC 2 cut(s) 286, 442
PpuMI RGGWCCY 1 cut(s) 359
PshBI ATTAAT 1 cut(s) 453
Psp124BI GAGCTC 1 cut(s) 717
Psp1406I AACGTT 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 2 cut(s) 391, 774
PspGI CCWGG 2 cut(s) 391, 774
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 3 cut(s) 359, 386, 1099
PspPPI RGGWCCY 1 cut(s) 359
PsrI GAACNNNNNNTAC 2 cut(s) 33, 65
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 1 cut(s) 1043
RsaNI GTAC 1 cut(s) 1042
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 717
SalI GTCGAC 1 cut(s) 595
SaqAI TTAA 4 cut(s) 285, 453, 834, 1052
SatI GCNGC 7 cut(s) 69, 129, 693, 906, 1032, 1107, 1142
Sau3AI GATC 4 cut(s) 8, 756, 841, 875
Sau96I GGNCC 3 cut(s) 359, 386, 1099
SchI GAGTC 2 cut(s) 287, 443
ScrFI CCNGG 2 cut(s) 393, 776
SduI GDGCHC 2 cut(s) 150, 717
SfaNI GCATC 3 cut(s) 484, 753, 1128
SfcI CTRYAG 1 cut(s) 660
Sfr274I CTCGAG 1 cut(s) 143
SinI GGWCC 3 cut(s) 359, 386, 1099
SlaI CTCGAG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 5 cut(s) 31, 44, 254, 282, 469
SsiI CCGC 1 cut(s) 129
SspI AATATT 1 cut(s) 792
SspMI CTAG 3 cut(s) 63, 932, 969
SstI GAGCTC 1 cut(s) 717
StyD4I CCNGG 2 cut(s) 391, 774
TaaI ACNGT 2 cut(s) 122, 594
TaiI ACGT 3 cut(s) 60, 96, 172
TaqI TCGA 5 cut(s) 144, 561, 596, 617, 717
TaqII GACCGA 1 cut(s) 644
TasI AATT 5 cut(s) 31, 44, 254, 282, 469
TatI WGTACW 1 cut(s) 1041
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 6 cut(s) 26, 310, 824, 1163, 1172, 1202
Tru1I TTAA 4 cut(s) 285, 453, 834, 1052
Tru9I TTAA 4 cut(s) 285, 453, 834, 1052
TscAI CASTG 1 cut(s) 1051
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 6 cut(s) 68, 692, 905, 1031, 1106, 1141
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 6 cut(s) 17, 25, 621, 638, 735, 1164
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 1 cut(s) 1051
VpaK11BI GGWCC 3 cut(s) 359, 386, 1099
VspI ATTAAT 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 1194
XapI RAATTY 2 cut(s) 31, 254
XhoI CTCGAG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 3 cut(s) 63, 932, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.