Rh1DG446700

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010923
Physical Location & Seq
Reverse (-)
65599651 .. 65602725
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG446700.1

Sequence Viewer

Length: 1059 bp
ATGGCGTCGCAGAGGGAAACCAAGTTCCTGCAGGAGCTCATTCTCTACGCCGCGAGCGCCGCCATGAGCTGCCTGGTTCTGTTCGTGGGGCTCCGACACCTCGACCCGAACCGCGAGGCCTCCAAGAAGGCCCTGGAGCACAAGAAGGAAATCGCTAGGCGCCTCGGCCGTCCTCTCATTCACACCAATCCTTATGAGGATGTCATAGCGTGCGATGTCATAAACCCTGACCACATTGATGTGGAGTTTAACTCCATTGGAGGACTGGAGGCTATAAAGGAAGCCTTGTTTGAACTGGTGATTCTTCCACTGAAGAGGCCGGACCTCTTTAACCATGGGAAACTCCTTGGTCCACAAAAGGGAGTCTTGTTGTACGGACCACCGGGTACTGGGAAGACTATGCTTGCTAAAGCTATTGCAAAGGAGTCTGGAGCTGTTTTCATTAATGTTAGGATATCAAACCTGATGAGCAAATGGTTTGGTGATGCACAAAAACTTGTTTCTGCTGTATTTAGCTTGGCTCATAAACTCCAGCCTGCTATCATATTTATCGACGAGGTTGATAGTTTCTTGGGACAGCGCCGTACTACAGAGCATGAAGCGTTAACAAACATGAAAACTGAGTTCATGGCTTTGTGGGATGGGTTTACTACAGATCAGCATGCTCGGGTTATGGTTCTCGCCGCTACCAATCGTCCATCAGAGCTCGATGAAGCAATACTTCGGCGTCTTCCTCAGGCCTTTGAGATTGGGATGCCCAATCAAAGGGATAGAGCTGAGATACTGAAGGTAGTCTTGAAGGGTGAGAGAGTTGAAAAGAACATTGATTATGACCGTTTAGCTGCCTTGGCTGAGGGTTACTCCGGTTCAGATCTACTTGAGCTCTGCAAGAAAGCAGCCTATTTCCCTATCAGAGACTTACTAGATGAAGAAAAGAAGGGGAAAAAATCTTCAGTGAGTTTCCTTTTCAACCATTTAACTGTTAACAAACCCTGCTATATAAAATCCAAGAAATCTTGCAGAAGTGGGGATATTTATTTTAGAATTGTAGGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

352

Amino Acids

39.53

Weight (kDa)

8.91

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_5 PF07728 121 - 244 1.3e-06 AAA domain (dynein-related subfamily)
AAA PF00004 122 - 251 6.1e-37 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 274 - 310 4.2e-10 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27680 AT5G53540
fragaria_vesca FvH4_7g32510
malus_domestica MD01G1225800.v1.1 MD07G1296300.v1.1
prunus_persica Prupe.2G315900_v2.0.a1
pyrus_communis pycom01g23430 pycom07g26910
rosa_chinensis RchiOBHm_Chr1g0382351
rosa_laevigata RLG00000026184
rosa_multiflora Rmu_sc0000110.1_g000041 Rmu_sc0006177.1_g000002 Rmu_sc0028160.1_g000002
rosa_roxburghii Rroxscaffold_4G00278170
rosa_rugosa Rorug01G0433600
rosa_samantha Rh1AG458300 Rh1BG414600 Rh1CG428700 Rh1DG446700
rosa_wichuraiana Rw1G041270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 159
AccII CGCG 2 cut(s) 53, 114
AciI CCGC 4 cut(s) 51, 60, 112, 684
AcoI YGGCCR 1 cut(s) 166
AcuI CTGAAG 3 cut(s) 332, 806, 936
AcyI GRCGYC 3 cut(s) 5, 160, 727
AfaI GTAC 3 cut(s) 374, 388, 586
AfiI CCNNNNNNNGG 3 cut(s) 359, 389, 765
AgsI TTSAA 4 cut(s) 293, 799, 815, 970
AjnI CCWGG 2 cut(s) 72, 132
AloI GAACNNNNNNTCC 2 cut(s) 8, 40
AluBI AGCT 9 cut(s) 37, 69, 413, 434, 516, 706, 776, 842, 883
AluI AGCT 9 cut(s) 37, 69, 413, 434, 516, 706, 776, 842, 883
Alw21I GWGCWC 4 cut(s) 39, 141, 708, 885
Alw26I GTCTC 1 cut(s) 909
Ama87I CYCGRG 1 cut(s) 666
AoxI GGCC 5 cut(s) 117, 129, 166, 317, 738
ApeKI GCWGC 3 cut(s) 69, 842, 896
AseI ATTAAT 1 cut(s) 444
AspLEI GCGC 3 cut(s) 59, 162, 582
AspS9I GGNCC 4 cut(s) 130, 322, 350, 377
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 3 cut(s) 310, 494, 815
AvaI CYCGRG 1 cut(s) 666
AvaII GGWCC 3 cut(s) 322, 350, 377
AxyI CCTNAGG 1 cut(s) 735
BanI GGYRCC 1 cut(s) 159
BanII GRGCYC 4 cut(s) 39, 93, 708, 885
BbsI GAAGAC 2 cut(s) 401, 722
Bbv12I GWGCWC 4 cut(s) 39, 141, 708, 885
BbvCI CCTCAGC 1 cut(s) 852
BbvI GCAGC 3 cut(s) 56, 829, 908
BccI CCATC 2 cut(s) 635, 706
BceAI ACGGC 2 cut(s) 153, 567
BciT130I CCWGG 2 cut(s) 74, 134
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 909
BfaI CTAG 2 cut(s) 156, 923
BfmI CTRYAG 3 cut(s) 29, 588, 651
BfoI RGCGCY 3 cut(s) 60, 163, 583
BglII AGATCT 1 cut(s) 871
BisI GCNGC 6 cut(s) 51, 60, 70, 684, 843, 897
BlsI GCNGC 6 cut(s) 52, 61, 71, 685, 844, 898
Bme1390I CCNGG 3 cut(s) 74, 134, 384
Bme18I GGWCC 3 cut(s) 322, 350, 377
BmeT110I CYCGRG 1 cut(s) 666
BmgT120I GGNCC 4 cut(s) 130, 322, 350, 377
BmiI GGNNCC 2 cut(s) 92, 161
BmrFI CCNGG 3 cut(s) 74, 134, 384
BmrI ACTGGG 1 cut(s) 399
BmsI GCATC 2 cut(s) 475, 744
BmuI ACTGGG 1 cut(s) 399
BpiI GAAGAC 2 cut(s) 401, 722
BplI GAGNNNNNCTC 4 cut(s) 236, 268, 845, 877
BpmI CTGGAG 4 cut(s) 155, 287, 450, 515
Bpu10I CCTNAGC 1 cut(s) 852
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 384
BsaHI GRCGYC 3 cut(s) 5, 160, 727
BsaJI CCNNGG 5 cut(s) 132, 163, 334, 346, 846
BsaWI WCCGGW 1 cut(s) 863
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 389, 765
Bse1I ACTGG 3 cut(s) 270, 300, 394
Bse21I CCTNAGG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 74, 134
BseDI CCNNGG 5 cut(s) 132, 163, 334, 346, 846
BseGI GGATG 3 cut(s) 205, 646, 759
BseLI CCNNNNNNNGG 3 cut(s) 359, 389, 765
BseMII CTCAG 4 cut(s) 612, 749, 768, 843
BseNI ACTGG 3 cut(s) 270, 300, 394
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 56, 829, 908
Bsh1236I CGCG 2 cut(s) 53, 114
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 5 cut(s) 119, 131, 168, 319, 740
BshNI GGYRCC 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 4 cut(s) 39, 141, 708, 885
BsiHKCI CYCGRG 1 cut(s) 666
BsiSI CCGG 3 cut(s) 320, 383, 864
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 3 cut(s) 359, 389, 765
BsmAI GTCTC 1 cut(s) 909
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 5 cut(s) 119, 131, 168, 319, 740
BsoBI CYCGRG 1 cut(s) 666
Bsp1286I GDGCHC 5 cut(s) 39, 93, 141, 708, 885
Bsp143I GATC 2 cut(s) 655, 871
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 4 cut(s) 51, 60, 112, 684
BspANI GGCC 5 cut(s) 119, 131, 168, 319, 740
BspCNI CTCAG 4 cut(s) 613, 748, 769, 844
BspFNI CGCG 2 cut(s) 53, 114
BspLI GGNNCC 2 cut(s) 92, 161
BspMAI CTGCAG 1 cut(s) 33
BspT107I GGYRCC 1 cut(s) 159
BsrI ACTGG 3 cut(s) 270, 300, 394
BssECI CCNNGG 5 cut(s) 132, 163, 334, 346, 846
BssMI GATC 2 cut(s) 655, 871
BssNI GRCGYC 3 cut(s) 5, 160, 727
BssT1I CCWWGG 3 cut(s) 334, 346, 846
Bst2UI CCWGG 2 cut(s) 74, 134
Bst4CI ACNGT 2 cut(s) 836, 982
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 3 cut(s) 5, 160, 727
BstC8I GCNNGC 5 cut(s) 55, 211, 405, 537, 663
BstDEI CTNAG 4 cut(s) 621, 735, 777, 852
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 3 cut(s) 205, 646, 759
BstFNI CGCG 2 cut(s) 53, 114
BstH2I RGCGCY 3 cut(s) 60, 163, 583
BstHHI GCGC 3 cut(s) 59, 162, 582
BstKTI GATC 2 cut(s) 658, 874
BstMAI GTCTC 1 cut(s) 909
BstMBI GATC 2 cut(s) 655, 871
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 56, 59, 848
BstNI CCWGG 2 cut(s) 74, 134
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 3 cut(s) 72, 132, 382
BstSFI CTRYAG 3 cut(s) 29, 588, 651
BstUI CGCG 2 cut(s) 53, 114
BstV1I GCAGC 3 cut(s) 56, 829, 908
BstV2I GAAGAC 2 cut(s) 401, 722
BstX2I RGATCY 1 cut(s) 871
BstYI RGATCY 1 cut(s) 871
BstZI CGGCCG 1 cut(s) 166
Bsu36I CCTNAGG 1 cut(s) 735
BsuRI GGCC 5 cut(s) 119, 131, 168, 319, 740
BtgI CCRYGG 1 cut(s) 334
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 3 cut(s) 205, 646, 759
BtsIMutI CAGTG 2 cut(s) 308, 960
Cac8I GCNNGC 5 cut(s) 55, 211, 405, 537, 663
CfoI GCGC 3 cut(s) 59, 162, 582
Cfr13I GGNCC 4 cut(s) 130, 322, 350, 377
CseI GACGC 1 cut(s) 716
Csp6I GTAC 3 cut(s) 373, 387, 585
CviAII CATG 6 cut(s) 64, 335, 596, 613, 628, 662
CviQI GTAC 3 cut(s) 373, 387, 585
DdeI CTNAG 4 cut(s) 621, 735, 777, 852
DinI GGCGCC 1 cut(s) 161
DpnI GATC 2 cut(s) 657, 873
DpnII GATC 2 cut(s) 655, 871
EaeI YGGCCR 1 cut(s) 166
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Ecl136II GAGCTC 3 cut(s) 37, 706, 883
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 334, 346, 846
Eco147I AGGCCT 2 cut(s) 119, 740
Eco24I GRGCYC 4 cut(s) 39, 93, 708, 885
Eco32I GATATC 1 cut(s) 456
Eco47I GGWCC 3 cut(s) 322, 350, 377
Eco52I CGGCCG 1 cut(s) 166
Eco53kI GAGCTC 3 cut(s) 37, 706, 883
Eco57I CTGAAG 3 cut(s) 332, 806, 936
Eco81I CCTNAGG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 666
EcoICRI GAGCTC 3 cut(s) 37, 706, 883
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 72, 132
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 3 cut(s) 334, 346, 846
EcoT38I GRGCYC 4 cut(s) 39, 93, 708, 885
EgeI GGCGCC 1 cut(s) 161
EheI GGCGCC 1 cut(s) 161
ErhI CCWWGG 3 cut(s) 334, 346, 846
FaeI CATG 6 cut(s) 67, 338, 599, 616, 631, 665
FalI AAGNNNNNCTT 8 cut(s) 269, 301, 350, 382, 705, 737, 779, 811
FaqI GGGAC 1 cut(s) 588
FatI CATG 6 cut(s) 63, 334, 595, 612, 627, 661
Fnu4HI GCNGC 6 cut(s) 51, 60, 70, 684, 843, 897
FokI GGATG 3 cut(s) 212, 653, 766
FriOI GRGCYC 4 cut(s) 39, 93, 708, 885
Fsp4HI GCNGC 6 cut(s) 51, 60, 70, 684, 843, 897
FspBI CTAG 2 cut(s) 156, 923
GlaI GCGC 3 cut(s) 58, 161, 581
GluI GCNGC 6 cut(s) 51, 60, 70, 684, 843, 897
GsuI CTGGAG 4 cut(s) 155, 287, 450, 515
HaeII RGCGCY 3 cut(s) 60, 163, 583
HaeIII GGCC 5 cut(s) 119, 131, 168, 319, 740
HapII CCGG 3 cut(s) 320, 383, 864
HgaI GACGC 1 cut(s) 716
HhaI GCGC 3 cut(s) 59, 162, 582
Hin1I GRCGYC 3 cut(s) 5, 160, 727
Hin1II CATG 6 cut(s) 67, 338, 599, 616, 631, 665
Hin6I GCGC 3 cut(s) 57, 160, 580
HinP1I GCGC 3 cut(s) 57, 160, 580
HincII GTYRAC 2 cut(s) 606, 985
HindII GTYRAC 2 cut(s) 606, 985
HinfI GANTC 4 cut(s) 301, 363, 425, 1053
HpaI GTTAAC 2 cut(s) 606, 985
HpaII CCGG 3 cut(s) 320, 383, 864
HphI GGTGA 3 cut(s) 310, 494, 815
Hpy166II GTNNAC 4 cut(s) 353, 606, 648, 985
Hpy188I TCNGA 4 cut(s) 95, 703, 871, 914
Hpy188III TCNNGA 2 cut(s) 429, 796
Hpy8I GTNNAC 4 cut(s) 353, 606, 648, 985
Hpy99I CGWCG 2 cut(s) 10, 557
HpyAV CCTTC 5 cut(s) 121, 139, 781, 793, 931
HpyCH4III ACNGT 2 cut(s) 836, 982
HpyCH4V TGCA 5 cut(s) 31, 419, 488, 888, 1020
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 59, 848
HpyF3I CTNAG 4 cut(s) 621, 735, 777, 852
Hsp92I GRCGYC 3 cut(s) 5, 160, 727
Hsp92II CATG 6 cut(s) 67, 338, 599, 616, 631, 665
HspAI GCGC 3 cut(s) 57, 160, 580
KasI GGCGCC 1 cut(s) 159
KspAI GTTAAC 2 cut(s) 606, 985
Kzo9I GATC 2 cut(s) 655, 871
LmnI GCTCC 4 cut(s) 34, 96, 136, 431
Lsp1109I GCAGC 3 cut(s) 56, 829, 908
LweI GCATC 2 cut(s) 475, 744
MaeI CTAG 2 cut(s) 156, 923
MaeIII GTNAC 1 cut(s) 857
MalI GATC 2 cut(s) 657, 873
MboI GATC 2 cut(s) 655, 871
MboII GAAGA 6 cut(s) 296, 325, 406, 722, 941, 942
MflI RGATCY 1 cut(s) 871
MhlI GDGCHC 5 cut(s) 39, 93, 141, 708, 885
MluCI AATT 1 cut(s) 1044
Mly113I GGCGCC 1 cut(s) 160
MlyI GAGTC 2 cut(s) 372, 434
MmeI TCCRAC 1 cut(s) 118
MseI TTAA 6 cut(s) 249, 330, 444, 605, 977, 984
MslI CAYNNNNRTG 2 cut(s) 237, 239
MspI CCGG 3 cut(s) 320, 383, 864
MspR9I CCNGG 3 cut(s) 74, 134, 384
MvaI CCWGG 2 cut(s) 74, 134
MvnI CGCG 2 cut(s) 53, 114
MwoI GCNNNNNNNGC 3 cut(s) 56, 59, 848
NarI GGCGCC 1 cut(s) 160
NciI CCSGG 1 cut(s) 384
NcoI CCATGG 1 cut(s) 334
NdeII GATC 2 cut(s) 655, 871
NlaIII CATG 6 cut(s) 67, 338, 599, 616, 631, 665
NlaIV GGNNCC 2 cut(s) 92, 161
NmeAIII GCCGAG 1 cut(s) 144
NspI RCATGY 1 cut(s) 665
PaeI GCATGC 1 cut(s) 665
PceI AGGCCT 2 cut(s) 119, 740
PfeI GAWTC 2 cut(s) 301, 1053
PkrI GCNGC 6 cut(s) 52, 61, 71, 685, 844, 898
PleI GAGTC 2 cut(s) 371, 433
PluTI GGCGCC 1 cut(s) 163
PpsI GAGTC 2 cut(s) 371, 433
PshBI ATTAAT 1 cut(s) 444
Psp124BI GAGCTC 3 cut(s) 39, 708, 885
Psp6I CCWGG 2 cut(s) 72, 132
PspGI CCWGG 2 cut(s) 72, 132
PspN4I GGNNCC 2 cut(s) 92, 161
PspPI GGNCC 4 cut(s) 130, 322, 350, 377
PstI CTGCAG 1 cut(s) 33
PsuI RGATCY 1 cut(s) 871
RsaI GTAC 3 cut(s) 374, 388, 586
RsaNI GTAC 3 cut(s) 373, 387, 585
RseI CAYNNNNRTG 2 cut(s) 237, 239
SacI GAGCTC 3 cut(s) 39, 708, 885
SaqAI TTAA 6 cut(s) 249, 330, 444, 605, 977, 984
SatI GCNGC 6 cut(s) 51, 60, 70, 684, 843, 897
Sau3AI GATC 2 cut(s) 655, 871
Sau96I GGNCC 4 cut(s) 130, 322, 350, 377
SbfI CCTGCAGG 1 cut(s) 33
SchI GAGTC 2 cut(s) 372, 434
ScrFI CCNGG 3 cut(s) 74, 134, 384
SdaI CCTGCAGG 1 cut(s) 33
SduI GDGCHC 5 cut(s) 39, 93, 141, 708, 885
SfaNI GCATC 2 cut(s) 475, 744
SfcI CTRYAG 3 cut(s) 29, 588, 651
SfoI GGCGCC 1 cut(s) 161
SinI GGWCC 3 cut(s) 322, 350, 377
SmiMI CAYNNNNRTG 2 cut(s) 237, 239
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SphI GCATGC 1 cut(s) 665
Sse8387I CCTGCAGG 1 cut(s) 33
Sse9I AATT 1 cut(s) 1044
SseBI AGGCCT 2 cut(s) 119, 740
SsiI CCGC 4 cut(s) 51, 60, 112, 684
SspDI GGCGCC 1 cut(s) 159
SspMI CTAG 2 cut(s) 156, 923
SstI GAGCTC 3 cut(s) 39, 708, 885
StuI AGGCCT 2 cut(s) 119, 740
StyD4I CCNGG 3 cut(s) 72, 132, 382
StyI CCWWGG 3 cut(s) 334, 346, 846
TaaI ACNGT 2 cut(s) 836, 982
TaqI TCGA 3 cut(s) 102, 552, 708
TasI AATT 1 cut(s) 1044
TauI GCSGC 3 cut(s) 53, 62, 686
TfiI GAWTC 2 cut(s) 301, 1053
Tru1I TTAA 6 cut(s) 249, 330, 444, 605, 977, 984
Tru9I TTAA 6 cut(s) 249, 330, 444, 605, 977, 984
TscAI CASTG 2 cut(s) 315, 960
TseI GCWGC 3 cut(s) 69, 842, 896
TspDTI ATGAA 6 cut(s) 430, 612, 616, 629, 726, 942
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 2 cut(s) 315, 960
VpaK11BI GGWCC 3 cut(s) 322, 350, 377
VspI ATTAAT 1 cut(s) 444
XceI RCATGY 1 cut(s) 665
XcmI CCANNNNNNNNNTGG 3 cut(s) 70, 130, 262
XspI CTAG 2 cut(s) 156, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.