MD01G1225800.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010923
Physical Location & Seq
Reverse (-)
31642720 .. 31645759
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1225800.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGGGGGTTCACGGGAGAGGGAGACCAAGTTCGTCCAGGAACTGGTACTGTACGCGGCAAGCGCGGCGTTGAGTTGCTTGGTTTTGTTCGTGGGGCTCCGGTATCTTGATCCGAACCGGGAAGCGTCTAAGAAGGCCTTGGAGAACAAGAAGGAGATCGCCAAGCGACTCGGCCGACCGCTTATTAGCACCAACACTTACGAGGATGTTATAGCCGGCGATGTTATAAACCCCGACGACATTGAAGTAGAATTTGGATCTATTGGAGGACTAGATGCTATCAAGGAAGCCTTGTGTGAACTAGTGATCCTTCCGCTACAGAGGCCTGATTTGTTTTCCCACGGGAAGCTCCTTGGTCCACAAAAAGGTGTCTTGCTGTATGGACCGCCGGGTACTGGAAAGACAATGCTTGCCAAAGCTATTGCAAAGGAGTCTGGAGCTGTTTTCATCAATGTCAGGATATCAAATCTGATGAGCAAATGGTTTGGTGATGCACAAAAGCTCGTTGCTGCTGTGTTTAGCTTGGCTTATAAACTCCAGCCTGCTATCATCTTTATTGATGAGGTTGATAGTTTCTTGGGTCAGCGCCGAACTACAGATCACGAGGCATTGACAAACATGAAAACTGAGTTCATGGCTTTATGGGATGGCTTTACTACAGATCAGAACGCTCGAGTTATGGTTCTCACTGCTACTAATCGTCCGTCAGAACTTGATGAAGCAATACTTCGGCGCCTTCCTCAAGCCTTTGAGATTGGGATGCCCGATAAAAGGGAGAGGGCTCAGATACTAAAGGTGATTTTGAAGGGTGAGAGGATTGAAGACAATATTGATTATGATCGTTTAGCCGTCTTGTCTGAGGGTTACACAGGTTCGGATCTACTTGAGCTTTGCAAAAAAGCAGCATATTTTCCTATCAGAGACCTACTAAATGAAGAAAAGACCGGAAAGAAATCTTCTGCACCAAGGCCGTTAACCCAGTTAGATTTGGAGAATGTTCTCGCGACTTCCAAACATACAAAGGTTGCTGCAAATGACTATACTGGGTTGTTCTCATCACAATCATTACCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

359

Amino Acids

39.65

Weight (kDa)

6.4

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvB_N PF05496 123 - 192 9.6e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 124 - 253 2.9e-36 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 276 - 312 1.5e-09 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27680 AT5G53540
fragaria_vesca FvH4_7g32510
malus_domestica MD01G1225800.v1.1 MD07G1296300.v1.1
prunus_persica Prupe.2G315900_v2.0.a1
pyrus_communis pycom01g23430 pycom07g26910
rosa_chinensis RchiOBHm_Chr1g0382351
rosa_laevigata RLG00000026184
rosa_multiflora Rmu_sc0000110.1_g000041 Rmu_sc0006177.1_g000002 Rmu_sc0028160.1_g000002
rosa_roxburghii Rroxscaffold_4G00278170
rosa_rugosa Rorug01G0433600
rosa_samantha Rh1AG458300 Rh1BG414600 Rh1CG428700 Rh1DG446700
rosa_wichuraiana Rw1G041270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 227, 531
AccB1I GGYRCC 1 cut(s) 732
AccB7I CCANNNNNTGG 1 cut(s) 43
AccII CGCG 3 cut(s) 56, 65, 1004
AciI CCGC 5 cut(s) 56, 65, 179, 314, 386
AclWI GGATC 4 cut(s) 104, 265, 301, 885
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 733
AfaI GTAC 3 cut(s) 48, 53, 394
AfiI CCNNNNNNNGG 4 cut(s) 43, 365, 395, 771
AgsI TTSAA 3 cut(s) 245, 805, 821
AhlI ACTAGT 1 cut(s) 301
AjnI CCWGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AloI GAACNNNNNNTCC 4 cut(s) 14, 46, 291, 323
AluBI AGCT 6 cut(s) 349, 419, 440, 502, 522, 889
AluI AGCT 6 cut(s) 349, 419, 440, 502, 522, 889
Alw26I GTCTC 2 cut(s) 17, 915
AlwI GGATC 4 cut(s) 104, 265, 301, 885
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 672
AoxI GGCC 4 cut(s) 135, 172, 323, 968
ApeKI GCWGC 3 cut(s) 509, 902, 1028
ApoI RAATTY 1 cut(s) 251
AspLEI GCGC 3 cut(s) 65, 588, 735
AspS9I GGNCC 2 cut(s) 356, 383
AsuC2I CCSGG 2 cut(s) 119, 390
AsuHPI GGTGA 3 cut(s) 500, 808, 821
AvaI CYCGRG 1 cut(s) 672
AvaII GGWCC 2 cut(s) 356, 383
BanI GGYRCC 1 cut(s) 732
BanII GRGCYC 2 cut(s) 99, 784
BauI CACGAG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 828
BbvI GCAGC 3 cut(s) 496, 914, 1015
BccI CCATC 1 cut(s) 641
BceAI ACGGC 2 cut(s) 833, 955
BciT130I CCWGG 1 cut(s) 38
BcnI CCSGG 2 cut(s) 119, 390
BcoDI GTCTC 2 cut(s) 17, 915
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 2 cut(s) 272, 302
BfmI CTRYAG 3 cut(s) 317, 594, 657
BfoI RGCGCY 2 cut(s) 589, 736
BisI GCNGC 5 cut(s) 57, 66, 510, 903, 1029
BlsI GCNGC 5 cut(s) 58, 67, 511, 904, 1030
Bme1390I CCNGG 3 cut(s) 38, 119, 390
Bme18I GGWCC 2 cut(s) 356, 383
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 2 cut(s) 356, 383
BmiI GGNNCC 2 cut(s) 98, 734
BmrFI CCNGG 3 cut(s) 38, 119, 390
BmrI ACTGGG 2 cut(s) 973, 1053
BmsI GCATC 3 cut(s) 265, 481, 750
BmuI ACTGGG 2 cut(s) 973, 1053
BpiI GAAGAC 1 cut(s) 828
BpmI CTGGAG 2 cut(s) 456, 521
BpuEI CTTGAG 2 cut(s) 726, 905
BpuMI CCSGG 2 cut(s) 119, 390
BsaBI GATNNNNATC 1 cut(s) 837
BsaHI GRCGYC 1 cut(s) 733
BsaI GGTCTC 2 cut(s) 17, 915
BsaJI CCNNGG 5 cut(s) 138, 340, 352, 965, 1070
BsaWI WCCGGW 2 cut(s) 99, 944
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 365, 395, 771
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 4 cut(s) 48, 400, 979, 1048
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 5 cut(s) 138, 340, 352, 965, 1070
BseGI GGATG 3 cut(s) 211, 652, 765
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 4 cut(s) 43, 365, 395, 771
BseMII CTCAG 3 cut(s) 618, 797, 849
BseNI ACTGG 4 cut(s) 48, 400, 979, 1048
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 496, 914, 1015
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 3 cut(s) 56, 65, 1004
Bsh1285I CGRYCG 2 cut(s) 175, 179
BshFI GGCC 4 cut(s) 137, 174, 325, 970
BshNI GGYRCC 1 cut(s) 732
BsiEI CGRYCG 2 cut(s) 175, 179
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 5 cut(s) 100, 118, 216, 389, 945
BslI CCNNNNNNNGG 4 cut(s) 43, 365, 395, 771
BsmAI GTCTC 2 cut(s) 17, 915
BsnI GGCC 4 cut(s) 137, 174, 325, 970
Bso31I GGTCTC 2 cut(s) 17, 915
BsoBI CYCGRG 1 cut(s) 672
Bsp1286I GDGCHC 2 cut(s) 99, 784
Bsp143I GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
Bsp68I TCGCGA 1 cut(s) 1004
BspACI CCGC 5 cut(s) 56, 65, 179, 314, 386
BspANI GGCC 4 cut(s) 137, 174, 325, 970
BspCNI CTCAG 3 cut(s) 619, 796, 850
BspFNI CGCG 3 cut(s) 56, 65, 1004
BspLI GGNNCC 2 cut(s) 98, 734
BspPI GGATC 4 cut(s) 104, 265, 301, 885
BspT107I GGYRCC 1 cut(s) 732
BspTNI GGTCTC 2 cut(s) 17, 915
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 4 cut(s) 48, 400, 979, 1048
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 5 cut(s) 138, 340, 352, 965, 1070
BssMI GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
BssNI GRCGYC 1 cut(s) 733
BssSI CACGAG 1 cut(s) 602
BssT1I CCWWGG 4 cut(s) 138, 352, 965, 1070
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 733
BstC8I GCNNGC 4 cut(s) 61, 217, 411, 543
BstDEI CTNAG 4 cut(s) 129, 627, 783, 858
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 3 cut(s) 211, 652, 765
BstFNI CGCG 3 cut(s) 56, 65, 1004
BstH2I RGCGCY 2 cut(s) 589, 736
BstHHI GCGC 3 cut(s) 65, 588, 735
BstKTI GATC 8 cut(s) 112, 159, 260, 309, 601, 664, 841, 880
BstMAI GTCTC 2 cut(s) 17, 915
BstMBI GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
BstMCI CGRYCG 2 cut(s) 175, 179
BstMWI GCNNNNNNNGC 3 cut(s) 62, 65, 322
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 3 cut(s) 36, 117, 388
BstSFI CTRYAG 3 cut(s) 317, 594, 657
BstUI CGCG 3 cut(s) 56, 65, 1004
BstV1I GCAGC 3 cut(s) 496, 914, 1015
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 2 cut(s) 257, 877
BstYI RGATCY 2 cut(s) 257, 877
BstZI CGGCCG 1 cut(s) 172
BsuRI GGCC 4 cut(s) 137, 174, 325, 970
BtgI CCRYGG 1 cut(s) 340
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 3 cut(s) 211, 652, 765
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 1 cut(s) 687
BtuMI TCGCGA 1 cut(s) 1004
Cac8I GCNNGC 4 cut(s) 61, 217, 411, 543
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 3 cut(s) 65, 588, 735
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 2 cut(s) 356, 383
CseI GACGC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 47, 52, 393
CviAII CATG 2 cut(s) 619, 634
CviQI GTAC 3 cut(s) 47, 52, 393
DdeI CTNAG 4 cut(s) 129, 627, 783, 858
DinI GGCGCC 1 cut(s) 734
DpnI GATC 8 cut(s) 111, 158, 259, 308, 600, 663, 840, 879
DpnII GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
EaeI YGGCCR 1 cut(s) 172
EagI CGGCCG 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 4 cut(s) 138, 352, 965, 1070
Eco147I AGGCCT 2 cut(s) 137, 325
Eco24I GRGCYC 2 cut(s) 99, 784
Eco31I GGTCTC 2 cut(s) 17, 915
Eco32I GATATC 1 cut(s) 462
Eco47I GGWCC 2 cut(s) 356, 383
Eco52I CGGCCG 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 672
EcoRII CCWGG 1 cut(s) 36
EcoRV GATATC 1 cut(s) 462
EcoT14I CCWWGG 4 cut(s) 138, 352, 965, 1070
EcoT38I GRGCYC 2 cut(s) 99, 784
EgeI GGCGCC 1 cut(s) 734
EheI GGCGCC 1 cut(s) 734
ErhI CCWWGG 4 cut(s) 138, 352, 965, 1070
FaeI CATG 2 cut(s) 622, 637
FalI AAGNNNNNCTT 6 cut(s) 122, 154, 275, 307, 711, 743
FatI CATG 2 cut(s) 618, 633
Fnu4HI GCNGC 5 cut(s) 57, 66, 510, 903, 1029
FokI GGATG 3 cut(s) 218, 659, 772
FriOI GRGCYC 2 cut(s) 99, 784
Fsp4HI GCNGC 5 cut(s) 57, 66, 510, 903, 1029
FspBI CTAG 2 cut(s) 272, 302
GlaI GCGC 3 cut(s) 64, 587, 734
GluI GCNGC 5 cut(s) 57, 66, 510, 903, 1029
GsuI CTGGAG 2 cut(s) 456, 521
HaeII RGCGCY 2 cut(s) 589, 736
HaeIII GGCC 4 cut(s) 137, 174, 325, 970
HapII CCGG 5 cut(s) 100, 118, 216, 389, 945
HgaI GACGC 1 cut(s) 114
HhaI GCGC 3 cut(s) 65, 588, 735
Hin1I GRCGYC 1 cut(s) 733
Hin1II CATG 2 cut(s) 622, 637
Hin6I GCGC 3 cut(s) 63, 586, 733
HinP1I GCGC 3 cut(s) 63, 586, 733
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HinfI GANTC 2 cut(s) 168, 431
HpaI GTTAAC 1 cut(s) 975
HpaII CCGG 5 cut(s) 100, 118, 216, 389, 945
HphI GGTGA 3 cut(s) 500, 808, 821
Hpy166II GTNNAC 4 cut(s) 11, 299, 359, 975
Hpy188I TCNGA 8 cut(s) 114, 471, 666, 709, 786, 859, 877, 920
Hpy188III TCNNGA 5 cut(s) 107, 435, 457, 602, 1003
Hpy8I GTNNAC 4 cut(s) 11, 299, 359, 975
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 5 cut(s) 127, 145, 320, 746, 799
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4V TGCA 5 cut(s) 425, 494, 894, 962, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 65, 322
HpyF3I CTNAG 4 cut(s) 129, 627, 783, 858
Hsp92I GRCGYC 1 cut(s) 733
Hsp92II CATG 2 cut(s) 622, 637
HspAI GCGC 3 cut(s) 63, 586, 733
KasI GGCGCC 1 cut(s) 732
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
KspAI GTTAAC 1 cut(s) 975
Kzo9I GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
LmnI GCTCC 3 cut(s) 102, 354, 437
Lsp1109I GCAGC 3 cut(s) 496, 914, 1015
LweI GCATC 3 cut(s) 265, 481, 750
MaeI CTAG 2 cut(s) 272, 302
MaeIII GTNAC 1 cut(s) 863
MalI GATC 8 cut(s) 111, 158, 259, 308, 600, 663, 840, 879
MboI GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
MboII GAAGA 3 cut(s) 833, 947, 948
MflI RGATCY 2 cut(s) 257, 877
MhlI GDGCHC 2 cut(s) 99, 784
MluCI AATT 1 cut(s) 251
Mly113I GGCGCC 1 cut(s) 733
MlyI GAGTC 2 cut(s) 162, 440
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 1 cut(s) 974
MspI CCGG 5 cut(s) 100, 118, 216, 389, 945
MspR9I CCNGG 3 cut(s) 38, 119, 390
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 3 cut(s) 56, 65, 1004
MwoI GCNNNNNNNGC 3 cut(s) 62, 65, 322
NaeI GCCGGC 1 cut(s) 217
NarI GGCGCC 1 cut(s) 733
NciI CCSGG 2 cut(s) 119, 390
NdeII GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 2 cut(s) 622, 637
NlaIV GGNNCC 2 cut(s) 98, 734
NmeAIII GCCGAG 1 cut(s) 150
NruI TCGCGA 1 cut(s) 1004
PaeR7I CTCGAG 1 cut(s) 672
PceI AGGCCT 2 cut(s) 137, 325
PdiI GCCGGC 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 36
PkrI GCNGC 5 cut(s) 58, 67, 511, 904, 1030
PleI GAGTC 2 cut(s) 162, 439
PluTI GGCGCC 1 cut(s) 736
PpsI GAGTC 2 cut(s) 162, 439
PsiI TTATAA 2 cut(s) 227, 531
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 98, 734
PspPI GGNCC 2 cut(s) 356, 383
PspXI VCTCGAGB 1 cut(s) 672
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 2 cut(s) 257, 877
RruI TCGCGA 1 cut(s) 1004
RsaI GTAC 3 cut(s) 48, 53, 394
RsaNI GTAC 3 cut(s) 47, 52, 393
SaqAI TTAA 1 cut(s) 974
SatI GCNGC 5 cut(s) 57, 66, 510, 903, 1029
Sau3AI GATC 8 cut(s) 109, 156, 257, 306, 598, 661, 838, 877
Sau96I GGNCC 2 cut(s) 356, 383
SchI GAGTC 2 cut(s) 162, 440
ScrFI CCNGG 3 cut(s) 38, 119, 390
SduI GDGCHC 2 cut(s) 99, 784
SfaNI GCATC 3 cut(s) 265, 481, 750
SfcI CTRYAG 3 cut(s) 317, 594, 657
SfoI GGCGCC 1 cut(s) 734
Sfr274I CTCGAG 1 cut(s) 672
SinI GGWCC 2 cut(s) 356, 383
SlaI CTCGAG 1 cut(s) 672
SmlI CTYRAG 3 cut(s) 672, 741, 884
SmoI CTYRAG 3 cut(s) 672, 741, 884
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 1 cut(s) 251
SseBI AGGCCT 2 cut(s) 137, 325
SsiI CCGC 5 cut(s) 56, 65, 179, 314, 386
SspDI GGCGCC 1 cut(s) 732
SspI AATATT 1 cut(s) 829
SspMI CTAG 2 cut(s) 272, 302
StuI AGGCCT 2 cut(s) 137, 325
StyD4I CCNGG 3 cut(s) 36, 117, 388
StyI CCWWGG 4 cut(s) 138, 352, 965, 1070
TaaI ACNGT 1 cut(s) 51
TaqI TCGA 1 cut(s) 673
TasI AATT 1 cut(s) 251
TauI GCSGC 2 cut(s) 59, 68
Tru1I TTAA 1 cut(s) 974
Tru9I TTAA 1 cut(s) 974
TscAI CASTG 1 cut(s) 694
TseI GCWGC 3 cut(s) 509, 902, 1028
TspDTI ATGAA 5 cut(s) 436, 622, 635, 732, 948
TspGWI ACGGA 1 cut(s) 693
TspRI CASTG 1 cut(s) 694
Van91I CCANNNNNTGG 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 356, 383
XapI RAATTY 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 672
XspI CTAG 2 cut(s) 272, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.