Rroxscaffold_4G00278170

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010923
Physical Location & Seq
Forward (+)
1257883 .. 1263280
5398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00278170.1

Sequence Viewer

Length: 1170 bp
ATGCGTGTGGCTTCAACATGCCAGAAGAAACGTACACCCAGAAATGCGTGTGGCTTTTATATGGTCAACAACCCCAACAGCTATGGATTATGGAATAATGACTTGGATGCATTTGATTTGGAAGTAATCGAATCAGATGGTGAGACATCATTTGACAAACCTGAGGGAAACCAAGTTCCTGCAGGAGCTCATTCTCTACGCCGCGAGCGCCGCCATGAGCTGCCTGGTTCTGTTCGTGGGGCTCCGACACCTCGACCCGAACCGCGAGGCCTCAAGAAGGCCCTGGAGCACAAGAAGGAAATCGCTAGGCGCCTCGGCCGTCCTCTCATTCACACCAATCCTTATGAGGATGTCATAGCTTGCGATGTTATAAACCCTGACCACATTGATGTGGAGTTTAACTCCATTGGAGGACTGGAGGCTATAAAGGAAGCATTGTTTGAACTGGTGATTCTTCCACTGAAGAGGCCGGACCTCTTTAACCATGGGAAACTCCTTGGTCCACAAAAGGGAGTCTTGTTGTACGGACCACCGGGTACTGGGAAGACTATGCTTGCTAAAGCTATTGCAAAGGAGTCTGGAGCTGTTTTCATTAATGTTAGGATATCAAACCTGATGAGCAAATGGTTTGGTGATGCACAAAAACTTGTTTCTGCTGTGTTTAGCTTGGCTCATAAACTCCAGCCTGCTATCATATTTATCGACGAGGTTGATAGTTTCTTGGGACAGCGCCGTACTACAGAGCATGAAGCGTTAACAAACATGAAAACTGAGTTCATGGCTTTGTGGGATGGGTTTACTACAGACCAGCATGCTCGGGTTATGGTTCTCGCCGCTACCAATCGTCCATCAGAGCTTGATGAAGCAATACTTCGGCGTCTTCCTCAGGCCTTTGAGATTGGGATGCCCAATCAAAGGGATAGAGCTGAGATACTGAAGGCACCAAGGCCTTTAACACAGAAGGACTTGGAGAATGTTCTTGCAACTTCCAGGCATACAAAGGTTGCTGCAAATGACTATTATACGGGATTGAACTCACCATCATCATCGCCAGGGTTTTCAAGGAACAGGGAGTCCGGTGAGATTCCAGTTCAAGCTGCAATTAATGAGCTCTCTAAGCTTGTGGTTTCCCAAATATTGAATATTCAAACCGATCCCCAGGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA

Protein Analysis

389

Amino Acids

43.24

Weight (kDa)

7.76

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_5 PF07728 171 - 294 1.6e-06 AAA domain (dynein-related subfamily)
AAA PF00004 172 - 301 7.5e-37 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27680 AT5G53540
fragaria_vesca FvH4_7g32510
malus_domestica MD01G1225800.v1.1 MD07G1296300.v1.1
prunus_persica Prupe.2G315900_v2.0.a1
pyrus_communis pycom01g23430 pycom07g26910
rosa_chinensis RchiOBHm_Chr1g0382351
rosa_laevigata RLG00000026184
rosa_multiflora Rmu_sc0000110.1_g000041 Rmu_sc0006177.1_g000002 Rmu_sc0028160.1_g000002
rosa_roxburghii Rroxscaffold_4G00278170
rosa_rugosa Rorug01G0433600
rosa_samantha Rh1AG458300 Rh1BG414600 Rh1CG428700 Rh1DG446700
rosa_wichuraiana Rw1G041270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AccB1I GGYRCC 2 cut(s) 309, 940
AccII CGCG 2 cut(s) 204, 265
AciI CCGC 4 cut(s) 202, 211, 263, 834
AclWI GGATC 2 cut(s) 1148, 1157
AcoI YGGCCR 1 cut(s) 316
AcuI CTGAAG 2 cut(s) 482, 956
AcyI GRCGYC 2 cut(s) 310, 877
AfaI GTAC 4 cut(s) 34, 524, 538, 736
AfiI CCNNNNNNNGG 4 cut(s) 277, 509, 539, 915
AgsI TTSAA 7 cut(s) 15, 443, 1033, 1062, 1094, 1141, 1148
AjnI CCWGG 5 cut(s) 223, 282, 989, 1051, 1158
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AloI GAACNNNNNNTCC 4 cut(s) 159, 191, 1058, 1090
Alw21I GWGCWC 3 cut(s) 190, 291, 1113
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 2 cut(s) 1148, 1157
Ama87I CYCGRG 1 cut(s) 816
AoxI GGCC 6 cut(s) 268, 279, 316, 467, 888, 947
ApeKI GCWGC 3 cut(s) 220, 1007, 1097
AseI ATTAAT 2 cut(s) 594, 1104
AspLEI GCGC 3 cut(s) 210, 312, 732
AspS9I GGNCC 4 cut(s) 280, 472, 500, 527
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 5 cut(s) 152, 460, 644, 1029, 1091
AvaI CYCGRG 1 cut(s) 816
AvaII GGWCC 3 cut(s) 472, 500, 527
AxyI CCTNAGG 2 cut(s) 162, 885
BamHI GGATCC 1 cut(s) 1162
BanI GGYRCC 2 cut(s) 309, 940
BanII GRGCYC 3 cut(s) 190, 244, 1113
BbsI GAAGAC 2 cut(s) 551, 872
Bbv12I GWGCWC 3 cut(s) 190, 291, 1113
BbvI GCAGC 3 cut(s) 207, 994, 1084
BccI CCATC 4 cut(s) 131, 785, 856, 1048
BceAI ACGGC 2 cut(s) 303, 717
BciT130I CCWGG 5 cut(s) 225, 284, 991, 1053, 1160
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 3 cut(s) 180, 738, 801
BfoI RGCGCY 3 cut(s) 211, 313, 733
BisI GCNGC 6 cut(s) 202, 211, 221, 834, 1008, 1098
BlsI GCNGC 6 cut(s) 203, 212, 222, 835, 1009, 1099
Bme1390I CCNGG 6 cut(s) 225, 284, 534, 991, 1053, 1160
Bme18I GGWCC 3 cut(s) 472, 500, 527
BmeT110I CYCGRG 1 cut(s) 816
BmgT120I GGNCC 4 cut(s) 280, 472, 500, 527
BmiI GGNNCC 4 cut(s) 243, 311, 942, 1164
BmrFI CCNGG 6 cut(s) 225, 284, 534, 991, 1053, 1160
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 3 cut(s) 97, 625, 894
BmuI ACTGGG 1 cut(s) 549
BpiI GAAGAC 2 cut(s) 551, 872
BplI GAGNNNNNCTC 2 cut(s) 386, 418
BpmI CTGGAG 4 cut(s) 305, 437, 600, 665
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 534
BsaHI GRCGYC 2 cut(s) 310, 877
BsaJI CCNNGG 7 cut(s) 282, 313, 484, 496, 944, 1052, 1158
BsaWI WCCGGW 1 cut(s) 1076
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 509, 539, 915
Bse1I ACTGG 4 cut(s) 420, 450, 544, 1088
Bse21I CCTNAGG 2 cut(s) 162, 885
BseBI CCWGG 5 cut(s) 225, 284, 991, 1053, 1160
BseDI CCNNGG 7 cut(s) 282, 313, 484, 496, 944, 1052, 1158
BseGI GGATG 4 cut(s) 112, 355, 796, 909
BseLI CCNNNNNNNGG 4 cut(s) 277, 509, 539, 915
BseMII CTCAG 4 cut(s) 153, 762, 899, 918
BseNI ACTGG 4 cut(s) 420, 450, 544, 1088
BseX3I CGGCCG 1 cut(s) 316
BseXI GCAGC 3 cut(s) 207, 994, 1084
Bsh1236I CGCG 2 cut(s) 204, 265
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 6 cut(s) 270, 281, 318, 469, 890, 949
BshNI GGYRCC 2 cut(s) 309, 940
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 3 cut(s) 190, 291, 1113
BsiHKCI CYCGRG 1 cut(s) 816
BsiSI CCGG 3 cut(s) 470, 533, 1077
BslFI GGGAC 1 cut(s) 738
BslI CCNNNNNNNGG 4 cut(s) 277, 509, 539, 915
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 738
BsnI GGCC 6 cut(s) 270, 281, 318, 469, 890, 949
BsoBI CYCGRG 1 cut(s) 816
Bsp1286I GDGCHC 4 cut(s) 190, 244, 291, 1113
Bsp143I GATC 2 cut(s) 1153, 1162
Bsp19I CCATGG 1 cut(s) 484
BspACI CCGC 4 cut(s) 202, 211, 263, 834
BspANI GGCC 6 cut(s) 270, 281, 318, 469, 890, 949
BspCNI CTCAG 4 cut(s) 154, 763, 898, 919
BspFNI CGCG 2 cut(s) 204, 265
BspLI GGNNCC 4 cut(s) 243, 311, 942, 1164
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 2 cut(s) 1148, 1157
BspT107I GGYRCC 2 cut(s) 309, 940
BsrI ACTGG 4 cut(s) 420, 450, 544, 1088
BssECI CCNNGG 7 cut(s) 282, 313, 484, 496, 944, 1052, 1158
BssMI GATC 2 cut(s) 1153, 1162
BssNI GRCGYC 2 cut(s) 310, 877
BssT1I CCWWGG 3 cut(s) 484, 496, 944
Bst2UI CCWGG 5 cut(s) 225, 284, 991, 1053, 1160
Bst6I CTCTTC 1 cut(s) 458
BstACI GRCGYC 2 cut(s) 310, 877
BstC8I GCNNGC 5 cut(s) 206, 361, 555, 687, 813
BstDEI CTNAG 5 cut(s) 162, 771, 885, 927, 1116
BstDSI CCRYGG 1 cut(s) 484
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 4 cut(s) 112, 355, 796, 909
BstFNI CGCG 2 cut(s) 204, 265
BstH2I RGCGCY 3 cut(s) 211, 313, 733
BstHHI GCGC 3 cut(s) 210, 312, 732
BstKTI GATC 2 cut(s) 1156, 1165
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 2 cut(s) 1153, 1162
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 3 cut(s) 207, 210, 1117
BstNI CCWGG 5 cut(s) 225, 284, 991, 1053, 1160
BstNSI RCATGY 2 cut(s) 21, 815
BstSCI CCNGG 6 cut(s) 223, 282, 532, 989, 1051, 1158
BstSFI CTRYAG 3 cut(s) 180, 738, 801
BstUI CGCG 2 cut(s) 204, 265
BstV1I GCAGC 3 cut(s) 207, 994, 1084
BstV2I GAAGAC 2 cut(s) 551, 872
BstX2I RGATCY 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1162
BstZI CGGCCG 1 cut(s) 316
Bsu36I CCTNAGG 2 cut(s) 162, 885
BsuRI GGCC 6 cut(s) 270, 281, 318, 469, 890, 949
BtgI CCRYGG 1 cut(s) 484
BtgZI GCGATG 2 cut(s) 378, 1032
BtsCI GGATG 4 cut(s) 112, 355, 796, 909
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 5 cut(s) 206, 361, 555, 687, 813
CfoI GCGC 3 cut(s) 210, 312, 732
Cfr13I GGNCC 4 cut(s) 280, 472, 500, 527
CseI GACGC 1 cut(s) 866
Csp6I GTAC 4 cut(s) 33, 523, 537, 735
CviAII CATG 7 cut(s) 18, 215, 485, 746, 763, 778, 812
CviQI GTAC 4 cut(s) 33, 523, 537, 735
DdeI CTNAG 5 cut(s) 162, 771, 885, 927, 1116
DinI GGCGCC 1 cut(s) 311
DpnI GATC 2 cut(s) 1155, 1164
DpnII GATC 2 cut(s) 1153, 1162
EaeI YGGCCR 1 cut(s) 316
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
Ecl136II GAGCTC 2 cut(s) 188, 1111
EclXI CGGCCG 1 cut(s) 316
Eco130I CCWWGG 3 cut(s) 484, 496, 944
Eco147I AGGCCT 3 cut(s) 270, 890, 949
Eco24I GRGCYC 3 cut(s) 190, 244, 1113
Eco32I GATATC 1 cut(s) 606
Eco47I GGWCC 3 cut(s) 472, 500, 527
Eco52I CGGCCG 1 cut(s) 316
Eco53kI GAGCTC 2 cut(s) 188, 1111
Eco57I CTGAAG 2 cut(s) 482, 956
Eco81I CCTNAGG 2 cut(s) 162, 885
Eco88I CYCGRG 1 cut(s) 816
EcoICRI GAGCTC 2 cut(s) 188, 1111
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 5 cut(s) 223, 282, 989, 1051, 1158
EcoRV GATATC 1 cut(s) 606
EcoT14I CCWWGG 3 cut(s) 484, 496, 944
EcoT22I ATGCAT 1 cut(s) 112
EcoT38I GRGCYC 3 cut(s) 190, 244, 1113
EgeI GGCGCC 1 cut(s) 311
EheI GGCGCC 1 cut(s) 311
ErhI CCWWGG 3 cut(s) 484, 496, 944
FaeI CATG 7 cut(s) 21, 218, 488, 749, 766, 781, 815
FalI AAGNNNNNCTT 4 cut(s) 500, 532, 855, 887
FaqI GGGAC 1 cut(s) 738
FatI CATG 7 cut(s) 17, 214, 484, 745, 762, 777, 811
Fnu4HI GCNGC 6 cut(s) 202, 211, 221, 834, 1008, 1098
FokI GGATG 4 cut(s) 119, 362, 803, 916
FriOI GRGCYC 3 cut(s) 190, 244, 1113
Fsp4HI GCNGC 6 cut(s) 202, 211, 221, 834, 1008, 1098
FspBI CTAG 1 cut(s) 306
GlaI GCGC 3 cut(s) 209, 311, 731
GluI GCNGC 6 cut(s) 202, 211, 221, 834, 1008, 1098
GsuI CTGGAG 4 cut(s) 305, 437, 600, 665
HaeII RGCGCY 3 cut(s) 211, 313, 733
HaeIII GGCC 6 cut(s) 270, 281, 318, 469, 890, 949
HapII CCGG 3 cut(s) 470, 533, 1077
HgaI GACGC 1 cut(s) 866
HhaI GCGC 3 cut(s) 210, 312, 732
Hin1I GRCGYC 2 cut(s) 310, 877
Hin1II CATG 7 cut(s) 21, 218, 488, 749, 766, 781, 815
Hin6I GCGC 3 cut(s) 208, 310, 730
HinP1I GCGC 3 cut(s) 208, 310, 730
HincII GTYRAC 2 cut(s) 67, 756
HindII GTYRAC 2 cut(s) 67, 756
HindIII AAGCTT 1 cut(s) 1118
HinfI GANTC 6 cut(s) 131, 451, 513, 575, 1073, 1084
HpaI GTTAAC 1 cut(s) 756
HpaII CCGG 3 cut(s) 470, 533, 1077
HphI GGTGA 5 cut(s) 152, 460, 644, 1029, 1091
Hpy166II GTNNAC 5 cut(s) 35, 67, 503, 756, 798
Hpy188I TCNGA 3 cut(s) 136, 246, 853
Hpy188III TCNNGA 2 cut(s) 274, 579
Hpy8I GTNNAC 5 cut(s) 35, 67, 503, 756, 798
Hpy99I CGWCG 1 cut(s) 707
HpyAV CCTTC 4 cut(s) 271, 289, 931, 955
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 7 cut(s) 110, 182, 569, 638, 983, 1010, 1100
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 210, 1117
HpyF3I CTNAG 5 cut(s) 162, 771, 885, 927, 1116
HpySE526I ACGT 1 cut(s) 31
Hsp92I GRCGYC 2 cut(s) 310, 877
Hsp92II CATG 7 cut(s) 21, 218, 488, 749, 766, 781, 815
HspAI GCGC 3 cut(s) 208, 310, 730
KasI GGCGCC 1 cut(s) 309
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 2 cut(s) 1153, 1162
LmnI GCTCC 4 cut(s) 185, 247, 286, 581
Lsp1109I GCAGC 3 cut(s) 207, 994, 1084
LweI GCATC 3 cut(s) 97, 625, 894
MaeI CTAG 1 cut(s) 306
MaeII ACGT 1 cut(s) 31
MalI GATC 2 cut(s) 1155, 1164
MboI GATC 2 cut(s) 1153, 1162
MboII GAAGA 5 cut(s) 37, 446, 475, 556, 872
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 4 cut(s) 190, 244, 291, 1113
MluCI AATT 1 cut(s) 1101
Mly113I GGCGCC 1 cut(s) 310
MlyI GAGTC 3 cut(s) 522, 584, 1082
MmeI TCCRAC 1 cut(s) 269
Mph1103I ATGCAT 1 cut(s) 112
MseI TTAA 6 cut(s) 399, 480, 594, 755, 953, 1104
MslI CAYNNNNRTG 2 cut(s) 387, 389
MspI CCGG 3 cut(s) 470, 533, 1077
MspR9I CCNGG 6 cut(s) 225, 284, 534, 991, 1053, 1160
MvaI CCWGG 5 cut(s) 225, 284, 991, 1053, 1160
MvnI CGCG 2 cut(s) 204, 265
MwoI GCNNNNNNNGC 3 cut(s) 207, 210, 1117
NarI GGCGCC 1 cut(s) 310
NciI CCSGG 1 cut(s) 534
NcoI CCATGG 1 cut(s) 484
NdeII GATC 2 cut(s) 1153, 1162
NlaIII CATG 7 cut(s) 21, 218, 488, 749, 766, 781, 815
NlaIV GGNNCC 4 cut(s) 243, 311, 942, 1164
NmeAIII GCCGAG 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 112
NspI RCATGY 2 cut(s) 21, 815
PaeI GCATGC 1 cut(s) 815
PceI AGGCCT 3 cut(s) 270, 890, 949
PfeI GAWTC 3 cut(s) 131, 451, 1084
PkrI GCNGC 6 cut(s) 203, 212, 222, 835, 1009, 1099
PleI GAGTC 3 cut(s) 521, 583, 1081
PluTI GGCGCC 1 cut(s) 313
PpsI GAGTC 3 cut(s) 521, 583, 1081
PshBI ATTAAT 2 cut(s) 594, 1104
PsiI TTATAA 1 cut(s) 371
Psp124BI GAGCTC 2 cut(s) 190, 1113
Psp6I CCWGG 5 cut(s) 223, 282, 989, 1051, 1158
PspGI CCWGG 5 cut(s) 223, 282, 989, 1051, 1158
PspN4I GGNNCC 4 cut(s) 243, 311, 942, 1164
PspPI GGNCC 4 cut(s) 280, 472, 500, 527
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 1162
RsaI GTAC 4 cut(s) 34, 524, 538, 736
RsaNI GTAC 4 cut(s) 33, 523, 537, 735
RseI CAYNNNNRTG 2 cut(s) 387, 389
SacI GAGCTC 2 cut(s) 190, 1113
SaqAI TTAA 6 cut(s) 399, 480, 594, 755, 953, 1104
SatI GCNGC 6 cut(s) 202, 211, 221, 834, 1008, 1098
Sau3AI GATC 2 cut(s) 1153, 1162
Sau96I GGNCC 4 cut(s) 280, 472, 500, 527
SbfI CCTGCAGG 1 cut(s) 184
SchI GAGTC 3 cut(s) 522, 584, 1082
ScrFI CCNGG 6 cut(s) 225, 284, 534, 991, 1053, 1160
SdaI CCTGCAGG 1 cut(s) 184
SduI GDGCHC 4 cut(s) 190, 244, 291, 1113
SfaNI GCATC 3 cut(s) 97, 625, 894
SfcI CTRYAG 3 cut(s) 180, 738, 801
SfoI GGCGCC 1 cut(s) 311
SinI GGWCC 3 cut(s) 472, 500, 527
SmiMI CAYNNNNRTG 2 cut(s) 387, 389
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SphI GCATGC 1 cut(s) 815
Sse8387I CCTGCAGG 1 cut(s) 184
Sse9I AATT 1 cut(s) 1101
SseBI AGGCCT 3 cut(s) 270, 890, 949
SsiI CCGC 4 cut(s) 202, 211, 263, 834
SspDI GGCGCC 1 cut(s) 309
SspI AATATT 2 cut(s) 1137, 1144
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 2 cut(s) 190, 1113
StuI AGGCCT 3 cut(s) 270, 890, 949
StyD4I CCNGG 6 cut(s) 223, 282, 532, 989, 1051, 1158
StyI CCWWGG 3 cut(s) 484, 496, 944
TaiI ACGT 1 cut(s) 34
TaqI TCGA 3 cut(s) 129, 253, 702
TasI AATT 1 cut(s) 1101
TauI GCSGC 3 cut(s) 204, 213, 836
TfiI GAWTC 3 cut(s) 131, 451, 1084
Tru1I TTAA 6 cut(s) 399, 480, 594, 755, 953, 1104
Tru9I TTAA 6 cut(s) 399, 480, 594, 755, 953, 1104
TscAI CASTG 1 cut(s) 465
TseI GCWGC 3 cut(s) 220, 1007, 1097
TspDTI ATGAA 5 cut(s) 580, 762, 766, 779, 876
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 1 cut(s) 465
VpaK11BI GGWCC 3 cut(s) 472, 500, 527
VspI ATTAAT 2 cut(s) 594, 1104
XagI CCTNNNNNAGG 1 cut(s) 275
XceI RCATGY 2 cut(s) 21, 815
XcmI CCANNNNNNNNNTGG 2 cut(s) 221, 412
XspI CTAG 1 cut(s) 306
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.