AT5G53920

Ribosomal protein L11 methyltransferase (PrmA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014127
Physical Location & Seq
Forward (+)
21891983 .. 21894267
2285 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53920.1

Sequence Viewer

Length: 1116 bp
ATGTTAGCTTGGAATTTCATCAAACACTTTCCGTGCAAGAATCTCTCCCGCCATATCCTCAGAGACAGCAGAGTCAGACCTCTCTGCTTCTTCACTTCTTCTACTCCTCCATCCTCCTTTTCAATCTTTGCGTCTCTCTCTACTTCTTCTTCTTCCTCCTCCTCATCTTCGTGCTTCACTGATGAGTCTTTCGCTGCACCTTACCTCTCAGTTCGTATCCACTGTCCAAAACATGCCCTCGATCCATTCTCGGAGGCACTTTTGTGTTTTGGAGCAAGCTCTGTAGCTGTAGATGAAGCCATCGAAGAAGCCGACCAAGATGAGACTTCTCTTGCTTCAAAAGAGATATGTATAGAATCAATATTTCCAGTGCATGAAGAGGTGAAAATGTGCATTTCACAAGCTGCAAATTCCATAGGCTTGAAAGAGATACCCAAATTCAAAGTTGAAATGGGAGATGAACTTGACTGGATTACCAAAAACCAGGATTTGTTTCAGCCTGTTGAAATCGCCGAGAGACTTTGGATTGTACCTGAGTGGACATCTCCTCCTGTCGCTGAAGCGGTAAACATAATTCTGAACCCTGGATTTGCGTTTGGGACTGGAGAACATCCTACTACTAAGCTGTGTCTATTGCTTCTACAAAGTTTGATAAAAGGCGGAGAAGCTTTCCTCGACTATGGTACCGGTTCAGGAATACTTGCAATTGCAGCCCTCAAGTTTGGTGCTGCATCATCCGTTGGTGTTGATATTGATCCTCTAGCAATAAACTCTGCAATTCATAATGCAGCTCTGAACAACATTCCGTTGGAGAAACTGGAGCTGCACCTTGCACCTATTGAGAATAGCTCTTCTGGAAGAGAAATACCATTGCAAAAGGAACAGTTTGATGTAGTCATTGCAAACATACTCTTGAACCCGGTTATGAATCTGGCGGATCATATCCTTTCATTCGTGAAACCTGGGGCAACCATTGGCATTTCTGGTATCTTATCTGAACAGCTTCCAAATGTAAGAGAACGGTACTCTCCCTTCTTGGAGGACATTTCTGTAGCCACAATAGGTGATTGGGTATGCATGAGTGGTACCAAGAAAGAGGAGTTTATTGATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PrmA

Protein Analysis

371

Amino Acids

40.37

Weight (kDa)

4.8

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 68 - 365 1e-90 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_23 PF13489 218 - 331 2.8e-06 Methyltransferase domain
Methyltransf_31 PF13847 219 - 313 1.6e-09 Methyltransferase domain
Methyltransf_25 PF13649 225 - 321 1.8e-06 Methyltransferase domain
Methyltransf_11 PF08241 225 - 312 2.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53920
fragaria_vesca FvH4_3g19490
malus_domestica MD11G1252500.v1.1
prunus_persica Prupe.4G176500_v2.0.a1 Prupe.4G176500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0032751
rosa_laevigata RLG00000033424
rosa_multiflora Rmu_sc0027546.1_g000001 Rmu_ssc0000293.1_g000003
rosa_roxburghii Rroxscaffold_1G00047350 Rroxscaffold_1G00047810
rosa_rugosa Rorug05G0133500
rosa_samantha Rh5AG226200 Rh5BG227300 Rh5CG255100 Rh5DG232000
rosa_wichuraiana Rw5G020740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc65I GGTACC 2 cut(s) 683, 1085
AccB1I GGYRCC 2 cut(s) 683, 1085
AciI CCGC 4 cut(s) 49, 563, 660, 935
AclWI GGATC 3 cut(s) 236, 749, 945
AcsI RAATTY 3 cut(s) 13, 409, 437
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 4 cut(s) 531, 685, 1025, 1087
AgeI ACCGGT 1 cut(s) 686
AgsI TTSAA 7 cut(s) 123, 339, 424, 442, 449, 506, 916
AjnI CCWGG 3 cut(s) 483, 583, 961
AleI CACNNNNGTG 1 cut(s) 262
Alw26I GTCTC 4 cut(s) 57, 138, 317, 511
AlwI GGATC 3 cut(s) 236, 749, 945
ApeKI GCWGC 6 cut(s) 194, 404, 710, 728, 788, 823
ApoI RAATTY 3 cut(s) 13, 409, 437
AsiGI ACCGGT 1 cut(s) 686
Asp700I GAANNNNTTC 1 cut(s) 1002
Asp718I GGTACC 2 cut(s) 683, 1085
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 2 cut(s) 394, 1076
BaeI ACNNNNGTAYC 2 cut(s) 667, 700
BanI GGYRCC 2 cut(s) 683, 1085
BarI GAAGNNNNNNTAC 2 cut(s) 1016, 1048
BbvI GCAGC 6 cut(s) 181, 391, 715, 722, 800, 810
BccI CCATC 2 cut(s) 118, 308
BciT130I CCWGG 3 cut(s) 485, 585, 963
BciVI GTATCC 1 cut(s) 227
BcnI CCSGG 1 cut(s) 920
BcoDI GTCTC 4 cut(s) 57, 138, 317, 511
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 3 cut(s) 282, 288, 1050
BfuI GTATCC 1 cut(s) 227
BisI GCNGC 6 cut(s) 195, 405, 711, 729, 789, 824
BlsI GCNGC 6 cut(s) 196, 406, 712, 730, 790, 825
Bme1390I CCNGG 4 cut(s) 485, 585, 920, 963
BmiI GGNNCC 2 cut(s) 685, 1087
BmrFI CCNGG 4 cut(s) 485, 585, 920, 963
BmsI GCATC 1 cut(s) 740
BplI GAGNNNNNCTC 2 cut(s) 833, 865
BpmI CTGGAG 2 cut(s) 624, 839
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 920
BsaBI GATNNNNATC 1 cut(s) 753
BsaJI CCNNGG 2 cut(s) 583, 962
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 2 cut(s) 532, 562
Bse118I RCCGGY 1 cut(s) 686
Bse1I ACTGG 4 cut(s) 368, 473, 607, 822
Bse3DI GCAATG 2 cut(s) 869, 897
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 3 cut(s) 485, 585, 963
BseDI CCNNGG 2 cut(s) 583, 962
BseGI GGATG 3 cut(s) 110, 610, 734
BseJI GATNNNNATC 1 cut(s) 753
BseMI GCAATG 2 cut(s) 869, 897
BseMII CTCAG 3 cut(s) 73, 222, 525
BseNI ACTGG 4 cut(s) 368, 473, 607, 822
BseRI GAGGAG 5 cut(s) 96, 148, 151, 537, 1112
BseXI GCAGC 6 cut(s) 181, 391, 715, 722, 800, 810
BsgI GTGCAG 2 cut(s) 180, 809
BshNI GGYRCC 2 cut(s) 683, 1085
BshTI ACCGGT 1 cut(s) 686
BsiSI CCGG 2 cut(s) 687, 920
BslFI GGGAC 1 cut(s) 613
BsmAI GTCTC 4 cut(s) 57, 138, 317, 511
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 613
Bsp143I GATC 3 cut(s) 241, 754, 937
BspACI CCGC 4 cut(s) 49, 563, 660, 935
BspCNI CTCAG 3 cut(s) 72, 221, 526
BspLI GGNNCC 2 cut(s) 685, 1087
BspPI GGATC 3 cut(s) 236, 749, 945
BspQI GCTCTTC 1 cut(s) 856
BspT107I GGYRCC 2 cut(s) 683, 1085
BsrDI GCAATG 2 cut(s) 869, 897
BsrFI RCCGGY 1 cut(s) 686
BsrI ACTGG 4 cut(s) 368, 473, 607, 822
BssAI RCCGGY 1 cut(s) 686
BssECI CCNNGG 2 cut(s) 583, 962
BssMI GATC 3 cut(s) 241, 754, 937
Bst2UI CCWGG 3 cut(s) 485, 585, 963
Bst4CI ACNGT 3 cut(s) 224, 885, 1023
Bst6I CTCTTC 3 cut(s) 372, 853, 856
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 4 cut(s) 59, 208, 534, 621
BstF5I GGATG 3 cut(s) 110, 610, 734
BstKTI GATC 3 cut(s) 244, 757, 940
BstMAI GTCTC 4 cut(s) 57, 138, 317, 511
BstMBI GATC 3 cut(s) 241, 754, 937
BstMWI GCNNNNNNNGC 1 cut(s) 710
BstNI CCWGG 3 cut(s) 485, 585, 963
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 4 cut(s) 483, 583, 918, 961
BstSFI CTRYAG 3 cut(s) 282, 288, 1050
BstV1I GCAGC 6 cut(s) 181, 391, 715, 722, 800, 810
BsuI GTATCC 1 cut(s) 227
BtsCI GGATG 3 cut(s) 110, 610, 734
BtsIMutI CAGTG 3 cut(s) 177, 220, 375
Cac8I GCNNGC 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 686
CseI GACGC 1 cut(s) 120
Csp6I GTAC 4 cut(s) 530, 684, 1024, 1086
CspAI ACCGGT 1 cut(s) 686
CviAII CATG 3 cut(s) 233, 374, 1078
CviQI GTAC 4 cut(s) 530, 684, 1024, 1086
DdeI CTNAG 4 cut(s) 59, 208, 534, 621
DpnI GATC 3 cut(s) 243, 756, 939
DpnII GATC 3 cut(s) 241, 754, 937
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1104I CTCTTC 3 cut(s) 372, 853, 856
EarI CTCTTC 3 cut(s) 372, 853, 856
EciI GGCGGA 2 cut(s) 675, 950
Eco57I CTGAAG 1 cut(s) 579
EcoRII CCWGG 3 cut(s) 483, 583, 961
EcoT22I ATGCAT 1 cut(s) 1079
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 3 cut(s) 236, 377, 1081
FaqI GGGAC 1 cut(s) 613
FatI CATG 3 cut(s) 232, 373, 1077
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 6 cut(s) 195, 405, 711, 729, 789, 824
FokI GGATG 3 cut(s) 97, 597, 721
Fsp4HI GCNGC 6 cut(s) 195, 405, 711, 729, 789, 824
FspBI CTAG 1 cut(s) 761
GluI GCNGC 6 cut(s) 195, 405, 711, 729, 789, 824
GsuI CTGGAG 2 cut(s) 624, 839
HapII CCGG 2 cut(s) 687, 920
HgaI GACGC 1 cut(s) 120
Hin1II CATG 3 cut(s) 236, 377, 1081
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 5 cut(s) 40, 72, 185, 356, 928
HpaII CCGG 2 cut(s) 687, 920
HphI GGTGA 2 cut(s) 394, 1076
Hpy166II GTNNAC 2 cut(s) 540, 568
Hpy188I TCNGA 6 cut(s) 62, 77, 253, 579, 795, 997
Hpy188III TCNNGA 4 cut(s) 693, 855, 913, 955
Hpy8I GTNNAC 2 cut(s) 540, 568
HpyAV CCTTC 1 cut(s) 1042
HpyCH4III ACNGT 3 cut(s) 224, 885, 1023
HpyF10VI GCNNNNNNNGC 1 cut(s) 710
HpyF3I CTNAG 4 cut(s) 59, 208, 534, 621
Hsp92II CATG 3 cut(s) 236, 377, 1081
KpnI GGTACC 2 cut(s) 687, 1089
Kzo9I GATC 3 cut(s) 241, 754, 937
LguI GCTCTTC 1 cut(s) 856
LmnI GCTCC 2 cut(s) 272, 820
Lsp1109I GCAGC 6 cut(s) 181, 391, 715, 722, 800, 810
LweI GCATC 1 cut(s) 740
MaeI CTAG 1 cut(s) 761
MalI GATC 3 cut(s) 243, 756, 939
MboI GATC 3 cut(s) 241, 754, 937
MfeI CAATTG 1 cut(s) 705
MluCI AATT 6 cut(s) 13, 409, 437, 573, 705, 777
MlyI GAGTC 2 cut(s) 81, 194
MmeI TCCRAC 1 cut(s) 789
Mph1103I ATGCAT 1 cut(s) 1079
MroXI GAANNNNTTC 1 cut(s) 1002
MslI CAYNNNNRTG 2 cut(s) 169, 262
MspI CCGG 2 cut(s) 687, 920
MspR9I CCNGG 4 cut(s) 485, 585, 920, 963
MunI CAATTG 1 cut(s) 705
MvaI CCWGG 3 cut(s) 485, 585, 963
MwoI GCNNNNNNNGC 1 cut(s) 710
NciI CCSGG 1 cut(s) 920
NdeII GATC 3 cut(s) 241, 754, 937
NlaIII CATG 3 cut(s) 236, 377, 1081
NlaIV GGNNCC 2 cut(s) 685, 1087
NmeAIII GCCGAG 1 cut(s) 538
NsiI ATGCAT 1 cut(s) 1079
NspI RCATGY 1 cut(s) 236
OliI CACNNNNGTG 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 856
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 3 cut(s) 40, 356, 928
PinAI ACCGGT 1 cut(s) 686
PkrI GCNGC 6 cut(s) 196, 406, 712, 730, 790, 825
PleI GAGTC 2 cut(s) 80, 193
PpsI GAGTC 2 cut(s) 80, 193
Psp6I CCWGG 3 cut(s) 483, 583, 961
PspGI CCWGG 3 cut(s) 483, 583, 961
PspN4I GGNNCC 2 cut(s) 685, 1087
RsaI GTAC 4 cut(s) 531, 685, 1025, 1087
RsaNI GTAC 4 cut(s) 530, 684, 1024, 1086
RseI CAYNNNNRTG 2 cut(s) 169, 262
SapI GCTCTTC 1 cut(s) 856
SatI GCNGC 6 cut(s) 195, 405, 711, 729, 789, 824
Sau3AI GATC 3 cut(s) 241, 754, 937
SchI GAGTC 2 cut(s) 81, 194
ScrFI CCNGG 4 cut(s) 485, 585, 920, 963
SfaNI GCATC 1 cut(s) 740
SfcI CTRYAG 3 cut(s) 282, 288, 1050
SmiMI CAYNNNNRTG 2 cut(s) 169, 262
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 6 cut(s) 13, 409, 437, 573, 705, 777
SsiI CCGC 4 cut(s) 49, 563, 660, 935
SspI AATATT 1 cut(s) 363
SspMI CTAG 1 cut(s) 761
StyD4I CCNGG 4 cut(s) 483, 583, 918, 961
TaaI ACNGT 3 cut(s) 224, 885, 1023
TaqI TCGA 3 cut(s) 240, 303, 675
TasI AATT 6 cut(s) 13, 409, 437, 573, 705, 777
TfiI GAWTC 3 cut(s) 40, 356, 928
TscAI CASTG 3 cut(s) 184, 227, 375
TseI GCWGC 6 cut(s) 194, 404, 710, 728, 788, 823
TspDTI ATGAA 7 cut(s) 7, 309, 390, 474, 770, 939, 941
TspGWI ACGGA 3 cut(s) 21, 727, 795
TspRI CASTG 3 cut(s) 184, 227, 375
XapI RAATTY 3 cut(s) 13, 409, 437
XceI RCATGY 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 1 cut(s) 761
Zsp2I ATGCAT 1 cut(s) 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.