Rroxscaffold_1G00047350

ribosomal protein L11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014127
Physical Location & Seq
Forward (+)
66898430 .. 66903301
4872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00047350.1

Sequence Viewer

Length: 1497 bp
ATGGCGTCATCCTCCGGTGGTCCGACGGTGGGACGAGCAGCGGCGGTGGAGGCCTACATCGACGGTGGAGTGGTGGGCGTGGTGGCTCAAAGAGAGAGAGAGAAAGAGAGCCCGAGACGAGTCTTCTGTTTTCCTAGCCTCAAAAGCAGCCACAGAGAACCCCTTCCCGCTACTTCACCAATGTCAGGCCGTCCTTTGCTCAAACTCCTTGCCTCCACTCTGCTCCCCTCTCGAGCTTTCACTCTCAGTCGCCCATTTCCCTCTCGCTTCTTCACTACCCGGAAGCCCAGATACCTCTCGTGGCCCAATTCACTAGCTGCTGCAAACATCTCCGCTTCTTCTTCAGAGACTCCCTCTGCCCAGCCCTTTGCTTTGCCTTTCCAATCTGTCAACATTCGCTGCCAAAGACATGTCTCTGTGAGCTTCTTTACCTTCTCTTTTTTGTTGAACTTTTGCTTTTCAGTTGAATATTTGGACTGGGTTTATGCTCATGATATGCTTCAAGAAGCTCTGTTATGCTTTGGTGCAAGTTCTACTAGTGTTGATGAAGACGATGACTGTGAGGATTCCGACGAGATTTGCATTACTTCCATATTTCCTGAAGGTGAAGATGTAAATACTTGCATTTCCTGTGCTGCTGATTCCATAAGCTTGAAAGAGATACCTAGTTATGAAGTTGCGATAGGTGAGCAGTACGACTGGATAAGGAAAACTCAGGAATCATTTCATCCTGTTGAAATTACTGAAGGACTCTGGATTGTGCCCGCCTGGAAAACTCCCCTCATTTGGAACCGGAGAGCATCCTACTACAAGGTTATGTCTGTTGCTGCTTCATGGCTTGATAAAGGGGGAGAACAATTCTTGGACTATGGCACAGGTTCTGGAATTCTTGCAATTGCTGCAGTTAAGTTTGGTGCTGCCTTATCAGTCGGAATTGATATAGATCCACAAGCAATTACATCTGCACACCAGAATGCTAATCTGAACAACATAGGACCTGAACAAATGCAATTATATCTAGTTCCGAGTGAAAATTGCTCAACTAAGACAGATCCGTGTGGAAATGTGGAAGAGCAGAGCGCATTTGTAGCGGAAGCCATCTCAGAAACAGACAAGTACGATGTACTAATTGCAAATATACTTTTAAATCCCCTAATGGATTTGGCAGATCAGATCGTCTCTTATGCAAAGCCTGGGGCAGTTATCGGTCTCTCCGGGATTCTCTCTGATCAGAGCGATATTCACAGTTCTTGGAAGGTGTATCGACTACAGAGATGGATGGTTGGGCCTGTGTCCATGGCAGAAAGAAGCAAAATGTTGCTGGCAACTGAGGAATCTCTCCCGTCTAAACCATCAGTGGAAGTACAATGTGACCAAACTGTTCCGACAGTTTTGCCGATCAGAGATGCTATTCTATATGAGGCTGTATGGTCTGGTCACTTTTACATCATACGTGCTGGCTGGCTTAACAATCCAAACAGGAGCATGGACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PrmA

Protein Analysis

498

Amino Acids

54.8

Weight (kDa)

4.89

Isoelectric Point (pI)

52.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PrmA PF06325 168 - 260 1.3e-09 Ribosomal protein L11 methyltransferase (PrmA)
PrmA PF06325 280 - 413 6.3e-31 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_23 PF13489 281 - 412 5.1e-06 Methyltransferase domain
Methyltransf_31 PF13847 282 - 332 6.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53920
fragaria_vesca FvH4_3g19490
malus_domestica MD11G1252500.v1.1
prunus_persica Prupe.4G176500_v2.0.a1 Prupe.4G176500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0032751
rosa_laevigata RLG00000033424
rosa_multiflora Rmu_sc0027546.1_g000001 Rmu_ssc0000293.1_g000003
rosa_roxburghii Rroxscaffold_1G00047350 Rroxscaffold_1G00047810
rosa_rugosa Rorug05G0133500
rosa_samantha Rh5AG226200 Rh5BG227300 Rh5CG255100 Rh5DG232000
rosa_wichuraiana Rw5G020740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 41, 44, 168, 333, 765, 1091
AclWI GGATC 2 cut(s) 938, 1046
AcsI RAATTY 1 cut(s) 885
AcuI CTGAAG 3 cut(s) 327, 621, 765
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 695, 1118, 1125, 1365
AfiI CCNNNNNNNGG 3 cut(s) 29, 185, 786
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 5 cut(s) 448, 467, 503, 655, 737
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 2 cut(s) 767, 1192
AluBI AGCT 5 cut(s) 236, 317, 423, 509, 651
AluI AGCT 5 cut(s) 236, 317, 423, 509, 651
Alw26I GTCTC 5 cut(s) 109, 341, 418, 1183, 1214
AlwI GGATC 2 cut(s) 938, 1046
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 2 cut(s) 112, 231
AoxI GGCC 4 cut(s) 51, 187, 302, 1286
ApeKI GCWGC 9 cut(s) 38, 147, 317, 320, 399, 635, 827, 899, 917
ApoI RAATTY 1 cut(s) 885
Asp700I GAANNNNTTC 2 cut(s) 162, 723
AspLEI GCGC 1 cut(s) 1082
AspS9I GGNCC 4 cut(s) 20, 303, 995, 1286
AsuC2I CCSGG 2 cut(s) 280, 1216
AsuHPI GGTGA 3 cut(s) 168, 617, 698
AvaI CYCGRG 2 cut(s) 112, 231
AvaII GGWCC 2 cut(s) 20, 995
BaeGI GKGCMC 1 cut(s) 765
BanII GRGCYC 1 cut(s) 113
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BauI CACGAG 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 115, 555
BbvI GCAGC 9 cut(s) 50, 159, 304, 307, 386, 622, 814, 886, 904
BccI CCATC 4 cut(s) 1106, 1269, 1273, 1360
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 1375, 1409
BciT130I CCWGG 2 cut(s) 769, 1194
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 2 cut(s) 280, 1216
BcoDI GTCTC 5 cut(s) 109, 341, 418, 1183, 1214
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 5 cut(s) 135, 314, 537, 666, 1019
BfmI CTRYAG 2 cut(s) 900, 1268
Bme1390I CCNGG 4 cut(s) 280, 769, 1194, 1216
Bme18I GGWCC 2 cut(s) 20, 995
BmeT110I CYCGRG 2 cut(s) 112, 231
BmgT120I GGNCC 4 cut(s) 20, 303, 995, 1286
BmiI GGNNCC 1 cut(s) 791
BmrFI CCNGG 4 cut(s) 280, 769, 1194, 1216
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 2 cut(s) 809, 1396
BmuI ACTGGG 1 cut(s) 487
BpiI GAAGAC 2 cut(s) 115, 555
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpuMI CCSGG 2 cut(s) 280, 1216
BsaAI YACGTR 1 cut(s) 1454
BsaBI GATNNNNATC 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1214
BsaJI CCNNGG 2 cut(s) 1193, 1296
BsaWI WCCGGW 2 cut(s) 14, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 185, 786
Bse1I ACTGG 2 cut(s) 482, 704
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 2 cut(s) 769, 1194
BseDI CCNNGG 2 cut(s) 1193, 1296
BseGI GGATG 4 cut(s) 8, 727, 800, 1284
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 3 cut(s) 29, 185, 786
BseMII CTCAG 4 cut(s) 259, 728, 1116, 1320
BseNI ACTGG 2 cut(s) 482, 704
BseSI GKGCMC 1 cut(s) 765
BseXI GCAGC 9 cut(s) 50, 159, 304, 307, 386, 622, 814, 886, 904
BseYI CCCAGC 1 cut(s) 360
BsgI GTGCAG 1 cut(s) 948
BshFI GGCC 4 cut(s) 53, 189, 304, 1288
BsiHKCI CYCGRG 2 cut(s) 112, 231
BsiSI CCGG 4 cut(s) 15, 280, 793, 1215
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 29, 185, 786
BsmAI GTCTC 5 cut(s) 109, 341, 418, 1183, 1214
BsmBI CGTCTC 2 cut(s) 109, 1183
BsmFI GGGAC 1 cut(s) 45
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 4 cut(s) 53, 189, 304, 1288
Bso31I GGTCTC 1 cut(s) 1214
BsoBI CYCGRG 2 cut(s) 112, 231
Bsp1286I GDGCHC 2 cut(s) 113, 765
Bsp143I GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
Bsp19I CCATGG 1 cut(s) 1296
BspACI CCGC 6 cut(s) 41, 44, 168, 333, 765, 1091
BspANI GGCC 4 cut(s) 53, 189, 304, 1288
BspCNI CTCAG 4 cut(s) 258, 727, 1115, 1321
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 791
BspMAI CTGCAG 1 cut(s) 904
BspPI GGATC 2 cut(s) 938, 1046
BspQI GCTCTTC 1 cut(s) 1065
BspTNI GGTCTC 1 cut(s) 1214
BsrI ACTGG 2 cut(s) 482, 704
BssECI CCNNGG 2 cut(s) 1193, 1296
BssMI GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 1296
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 769, 1194
Bst4CI ACNGT 6 cut(s) 28, 65, 560, 1247, 1381, 1390
Bst6I CTCTTC 1 cut(s) 1065
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 899
BstBAI YACGTR 1 cut(s) 1454
BstC8I GCNNGC 4 cut(s) 765, 1323, 1459, 1463
BstDEI CTNAG 5 cut(s) 245, 714, 1044, 1102, 1329
BstDSI CCRYGG 1 cut(s) 1296
BstF5I GGATG 4 cut(s) 8, 727, 800, 1284
BstHHI GCGC 1 cut(s) 1082
BstKTI GATC 6 cut(s) 946, 1054, 1171, 1176, 1231, 1401
BstMAI GTCTC 5 cut(s) 109, 341, 418, 1183, 1214
BstMBI GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
BstMWI GCNNNNNNNGC 4 cut(s) 50, 144, 899, 1088
BstNI CCWGG 2 cut(s) 769, 1194
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 4 cut(s) 278, 767, 1192, 1214
BstSFI CTRYAG 2 cut(s) 900, 1268
BstSLI GKGCMC 1 cut(s) 765
BstV1I GCAGC 9 cut(s) 50, 159, 304, 307, 386, 622, 814, 886, 904
BstV2I GAAGAC 2 cut(s) 115, 555
BstX2I RGATCY 2 cut(s) 943, 1051
BstYI RGATCY 2 cut(s) 943, 1051
BsuRI GGCC 4 cut(s) 53, 189, 304, 1288
BtgI CCRYGG 1 cut(s) 1296
BtsCI GGATG 4 cut(s) 8, 727, 800, 1284
BtsIMutI CAGTG 1 cut(s) 1362
Cac8I GCNNGC 4 cut(s) 765, 1323, 1459, 1463
CaiI CAGNNNCTG 1 cut(s) 881
CciI TCATGA 1 cut(s) 490
CfoI GCGC 1 cut(s) 1082
Cfr13I GGNCC 4 cut(s) 20, 303, 995, 1286
Csp6I GTAC 4 cut(s) 694, 1117, 1124, 1364
CviAII CATG 5 cut(s) 410, 491, 834, 1297, 1486
CviQI GTAC 4 cut(s) 694, 1117, 1124, 1364
DdeI CTNAG 5 cut(s) 245, 714, 1044, 1102, 1329
DpnI GATC 6 cut(s) 945, 1053, 1170, 1175, 1230, 1400
DpnII GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
DraI TTTAAA 1 cut(s) 1146
Eam1104I CTCTTC 1 cut(s) 1065
EarI CTCTTC 1 cut(s) 1065
Eco130I CCWWGG 1 cut(s) 1296
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 1214
Eco47I GGWCC 2 cut(s) 20, 995
Eco57I CTGAAG 3 cut(s) 327, 621, 765
Eco88I CYCGRG 2 cut(s) 112, 231
EcoO109I RGGNCCY 1 cut(s) 995
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 2 cut(s) 767, 1192
EcoT14I CCWWGG 1 cut(s) 1296
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 1296
Esp3I CGTCTC 2 cut(s) 109, 1183
FaeI CATG 5 cut(s) 413, 494, 837, 1300, 1489
FaqI GGGAC 1 cut(s) 45
FatI CATG 5 cut(s) 409, 490, 833, 1296, 1485
FauI CCCGC 2 cut(s) 175, 772
FbaI TGATCA 1 cut(s) 1228
FokI GGATG 3 cut(s) 714, 787, 1291
FriOI GRGCYC 1 cut(s) 113
FspBI CTAG 5 cut(s) 135, 314, 537, 666, 1019
GlaI GCGC 1 cut(s) 1081
GsaI CCCAGC 1 cut(s) 364
HaeIII GGCC 4 cut(s) 53, 189, 304, 1288
HapII CCGG 4 cut(s) 15, 280, 793, 1215
HhaI GCGC 1 cut(s) 1082
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 413, 494, 837, 1300, 1489
Hin6I GCGC 1 cut(s) 1080
HinP1I GCGC 1 cut(s) 1080
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 649
HinfI GANTC 8 cut(s) 120, 349, 566, 641, 719, 750, 1219, 1334
HpaII CCGG 4 cut(s) 15, 280, 793, 1215
HphI GGTGA 3 cut(s) 168, 617, 698
Hpy166II GTNNAC 1 cut(s) 391
Hpy188III TCNNGA 7 cut(s) 231, 491, 503, 599, 716, 754, 882
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 3 cut(s) 28, 65, 575
HpyAV CCTTC 5 cut(s) 173, 442, 596, 740, 1249
HpyCH4III ACNGT 6 cut(s) 28, 65, 560, 1247, 1381, 1390
HpyCH4IV ACGT 1 cut(s) 1453
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 144, 899, 1088
HpyF3I CTNAG 5 cut(s) 245, 714, 1044, 1102, 1329
HpySE526I ACGT 1 cut(s) 1453
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 413, 494, 837, 1300, 1489
HspAI GCGC 1 cut(s) 1080
Ksp22I TGATCA 1 cut(s) 1228
Kzo9I GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
LguI GCTCTTC 1 cut(s) 1065
LmnI GCTCC 2 cut(s) 228, 1482
Lsp1109I GCAGC 9 cut(s) 50, 159, 304, 307, 386, 622, 814, 886, 904
LweI GCATC 2 cut(s) 809, 1396
MaeI CTAG 5 cut(s) 135, 314, 537, 666, 1019
MaeII ACGT 1 cut(s) 1453
MaeIII GTNAC 2 cut(s) 1370, 1436
MalI GATC 6 cut(s) 945, 1053, 1170, 1175, 1230, 1400
MboI GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
MboII GAAGA 7 cut(s) 115, 262, 330, 333, 560, 620, 1082
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 2 cut(s) 943, 1051
MhlI GDGCHC 2 cut(s) 113, 765
MlyI GAGTC 3 cut(s) 129, 343, 744
MmeI TCCRAC 4 cut(s) 47, 594, 910, 1409
MroXI GAANNNNTTC 2 cut(s) 162, 723
MseI TTAA 3 cut(s) 906, 1145, 1467
MslI CAYNNNNRTG 1 cut(s) 972
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 4 cut(s) 15, 280, 793, 1215
MspR9I CCNGG 4 cut(s) 280, 769, 1194, 1216
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 2 cut(s) 769, 1194
MwoI GCNNNNNNNGC 4 cut(s) 50, 144, 899, 1088
NciI CCSGG 2 cut(s) 280, 1216
NcoI CCATGG 1 cut(s) 1296
NdeII GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
NlaIII CATG 5 cut(s) 413, 494, 837, 1300, 1489
NlaIV GGNNCC 1 cut(s) 791
NmuCI GTSAC 2 cut(s) 1370, 1436
NspI RCATGY 1 cut(s) 413
PaeR7I CTCGAG 1 cut(s) 231
PagI TCATGA 1 cut(s) 490
PceI AGGCCT 1 cut(s) 53
PciI ACATGT 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 1065
PctI GAATGC 1 cut(s) 979
PdmI GAANNNNTTC 2 cut(s) 162, 723
PfeI GAWTC 5 cut(s) 566, 641, 719, 1219, 1334
PfoI TCCNGGA 1 cut(s) 1214
PleI GAGTC 3 cut(s) 128, 343, 744
PpsI GAGTC 3 cut(s) 128, 343, 744
Ppu21I YACGTR 1 cut(s) 1454
PpuMI RGGWCCY 1 cut(s) 995
PscI ACATGT 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 995
Psp6I CCWGG 2 cut(s) 767, 1192
PspFI CCCAGC 1 cut(s) 360
PspGI CCWGG 2 cut(s) 767, 1192
PspN4I GGNNCC 1 cut(s) 791
PspPI GGNCC 4 cut(s) 20, 303, 995, 1286
PspPPI RGGWCCY 1 cut(s) 995
PstI CTGCAG 1 cut(s) 904
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 2 cut(s) 943, 1051
RsaI GTAC 4 cut(s) 695, 1118, 1125, 1365
RsaNI GTAC 4 cut(s) 694, 1117, 1124, 1364
RseI CAYNNNNRTG 1 cut(s) 972
SapI GCTCTTC 1 cut(s) 1065
SaqAI TTAA 3 cut(s) 906, 1145, 1467
Sau3AI GATC 6 cut(s) 943, 1051, 1168, 1173, 1228, 1398
Sau96I GGNCC 4 cut(s) 20, 303, 995, 1286
SchI GAGTC 3 cut(s) 129, 343, 744
ScrFI CCNGG 4 cut(s) 280, 769, 1194, 1216
SduI GDGCHC 2 cut(s) 113, 765
SfaNI GCATC 2 cut(s) 809, 1396
SfcI CTRYAG 2 cut(s) 900, 1268
Sfr274I CTCGAG 1 cut(s) 231
SinI GGWCC 2 cut(s) 20, 995
SlaI CTCGAG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 972
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SpeI ACTAGT 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 53
SsiI CCGC 6 cut(s) 41, 44, 168, 333, 765, 1091
SspI AATATT 1 cut(s) 470
SspMI CTAG 5 cut(s) 135, 314, 537, 666, 1019
StuI AGGCCT 1 cut(s) 53
StyD4I CCNGG 4 cut(s) 278, 767, 1192, 1214
StyI CCWWGG 1 cut(s) 1296
TaaI ACNGT 6 cut(s) 28, 65, 560, 1247, 1381, 1390
TaiI ACGT 1 cut(s) 1456
TaqI TCGA 3 cut(s) 60, 232, 1264
TaqII GACCGA 1 cut(s) 1196
TatI WGTACW 2 cut(s) 1123, 1363
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 5 cut(s) 566, 641, 719, 1219, 1334
Tru1I TTAA 3 cut(s) 906, 1145, 1467
Tru9I TTAA 3 cut(s) 906, 1145, 1467
TscAI CASTG 1 cut(s) 1362
TseFI GTSAC 2 cut(s) 1370, 1436
TseI GCWGC 9 cut(s) 38, 147, 317, 320, 399, 635, 827, 899, 917
Tsp45I GTSAC 2 cut(s) 1370, 1436
TspDTI ATGAA 4 cut(s) 561, 687, 716, 822
TspGWI ACGGA 1 cut(s) 1044
TspRI CASTG 1 cut(s) 1362
VpaK11BI GGWCC 2 cut(s) 20, 995
XapI RAATTY 1 cut(s) 885
XceI RCATGY 1 cut(s) 413
XhoI CTCGAG 1 cut(s) 231
XmnI GAANNNNTTC 2 cut(s) 162, 723
XspI CTAG 5 cut(s) 135, 314, 537, 666, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.