AT5G57320

Villin headpiece domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001569
Physical Location & Seq
Forward (+)
23211952 .. 23218041
6090 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57320.1

Sequence Viewer

Length: 2889 bp
ATGACGTTTTCCATGAGAGATTTAGATCAGGCTCTTCAAGGAGCTGGCCAGAAATCCGGGATTGAAATATGGCGCATCGAGAACTTCAAACCTGTCACTGTTCCACAAGAGTCTCATGGGAAATTTTTCACCGGGGATTCCTATATAGTCTTAAAGACCACAGCGTCAAGAAGCGGTTCTTTGCATCATGATATTCACTACTGGCTCGGTAAAGATTCCAGCCAGGATGAAGCAGGTGCTGTAGCCGTCATGACTGTTGAATTAGATTCAGCATTAGGTGGACGTGCTGTTCAGTACCGAGAAGTACAGGGTCACGAAACCGAGAAGTTTCTTTCCTACTTCAAACCTTGCATAATACCTCAAGAAGGTGGAGTTGCTTCAGGGTTCAACCATGTAAAGCCCGAGGAGCATCAGACGCGTCTGTATATCTGCAAAGGCAAACATGTCGTCCGTGTTAAAGAGGTTCCGTTTGTTCGATCGACTCTCAACCACGAAGACGTTTTTATTCTTGATACAGAGTCTAAAATTTTTCAATTCAGTGGTTCCAAGTCGAGTATTCAAGAAAGAGCAAAAGCTCTTGAGGTTGTTCAGTACATTAAAGACACTTACCATGATGGAAAGTGCGATATCGCAGCTGTTGAGGATGGGAGGATGATGGCTGATGCTGAAGCTGGAGAGTTTTGGGGCTTGTTTGGTGGGTTTGCTCCGCTTCCTAAGAAACCAGCAGTCAATGATGACGAAACCGCTGCATCTGATGGTATCAAACTTTTCAGTGTCGAGAAGGGACAGACAGATGCCGTAGAGGCTGAGTGTTTGACGAAAGAGCTTCTGGACACTAACAAATGTTATATTCTCGATTGCGGTCTTGAATTGTTCGTTTGGAAGGGACGAAGTACTTCAATCGACCAAAGAAAGAGCGCAACTGAAGCTGCAGAAGAATTTTTCCGTTCGTCTGAACCGCCAAAATCAAACCTGGTCAGTGTGATGGAAGGGTATGAAACAGTGATGTTCCGATCTAAGTTTGATTCATGGCCGGCTTCAAGTACCATAGCTGAGCCCCAACAAGGCAGAGGCAAAGTCGCAGCTCTTTTGCAGCGGCAAGGAGTTAACGTTCAAGGCCTGGTTAAGACTTCTTCTTCTTCTTCTAAAGACGAACCGAAGCCATACATTGATGGTACAGGAAATCTCCAGGTCTGGCGAATCAATTGTGAAGAAAAGATCCTTCTCGAAGCAGCAGAGCAATCAAAGTTCTATAGCGGGGATTGTTATATACTCCAGTACTCATACCCAGGAGAAGACAGAGAGGAACATCTAGTGGGTACTTGGTTTGGCAAGCAAAGCGTTGAGGAAGACAGAGCATCTGCTATTTCTTTGGCAAACAAGATGGTTGAATCCATGAAGTTTGTGCCGGCTCAGGCTCGCATTAATGAGGGAAAAGAGCCAATTCAGTTTTTTGTGATCATGCAAAGCTTCATCACATTTAAGGGTGGTGTAAGTGATGCTTTCAAAAAGTACATAGCTGAGAACGACATCCCTGATACTACTTACGAAGCAGAAGGTGTTGCACTGTTTCGGGTTCAAGGTTCTGGACCCGAGAACATGCAAGCCATACAGATAGAAGCGGCTTCCGCAGGACTAAATTCTTCGCACTGTTATATATTACACGGTGATTCTACTGTTTTCACTTGGTGTGGCAATCTTACTTCCTCGGAAGATCAAGAACTTATGGAAAGAATGTTGGATTTGATTAAGCCAAATGAACCCACTAAGGCACAAAAGGAAGGCTCAGAGTCTGAACAGTTTTGGGAATTATTAGGAGGCAAATCAGAATATCCGAGCCAAAAGATCAAAAGGGATGGAGAGAGTGATCCTCATCTCTTTTCTTGCACATATACAAACGAAAGTCTGAAGGCTACAGAGATCTTCAACTTCACTCAAGATGATTTGATGACTGAAGATATCTTCATACTTGATTGTCACACTGAGGTCTTTGTCTGGGTGGGACAACAAGTTGACCCAAAGAAGAAACCTCAAGCTTTGGACATTGGAGAGAACTTTCTTAAGCATGATTTCCTCCTAGAGAATCTAGCAAGTGAAACACCGATATACATTGTAACAGAGGGAAACGAACCTCCATTTTTCACTCGGTTCTTCACCTGGGACTCTTCTAAATCTGGAATGCATGGAGACTCTTTCCAGAGAAAGCTTGCGATCCTGACAAATAAAGGAAAGCCGCTTCTAGATAAACCTAAAAGGAGAGTCCCTGCATACAGTAGCAGGTCCACCGTTCCAGACAAATCGCAGCCTCGGTCCAGAAGTATGACTTTTAGTCCAGACAGGGCCCGCGTGAGGGGACGGTCTCCAGCTTTCAACGCACTTGCAGCAAACTTTGAGAAACTAAATATAAGAAACCAATCAACTCCACCACCTATGGTTAGTCCAATGGTCCGGAAGCTTTACCCGAAATCTCATGCCCCAGACCTCTCGAAGATAGCTCCTAAATCCGCTATAGCTGCCCGTACAGCGCTTTTTGAAAAACCTACTCCTACTTCTCAAGAACCACCTACTAGCCCCAGTTCTTCAGAAGCTACAAACCAAGCAGAAGCACCAAAATCGACATCAGAGACAAACGAGGAAGAAGCAATGAGCAGCATCAATGAAGACTCAAAAGAAGAAGAAGCAGAAGAAGAGAGCAGCCTCCCTACTTTCCCATATGAACGCCTTAAAACCGACTCAGAGGATCCTGTTTCAGATGTCGACCTCACTAGAAGAGAGGCATACTTGACTTCAGTAGAGTTCAAGGAGAAGTTTGAGATGACAAAGAATGAGTTCTACAAACTGCCTAAATGGAAACAAAACAAGCTCAAAATGTCTGTCAATCTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

962

Amino Acids

107.82

Weight (kDa)

5.25

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 29 - 111 1.2e-13 Gelsolin repeat
Gelsolin PF00626 149 - 214 1.8e-09 Gelsolin repeat
Gelsolin PF00626 267 - 331 2.7e-09 Gelsolin repeat
Gelsolin PF00626 643 - 714 2.2e-07 Gelsolin repeat
VHP PF02209 927 - 962 2.3e-14 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 163
Acc36I ACCTGC 2 cut(s) 224, 2268
AccI GTMKAC 1 cut(s) 2757
AccII CGCG 2 cut(s) 418, 2346
AccIII TCCGGA 1 cut(s) 2448
AclI AACGTT 1 cut(s) 1110
AclWI GGATC 5 cut(s) 1213, 1862, 2206, 2735, 2748
AcoI YGGCCR 2 cut(s) 46, 1031
AcsI RAATTY 4 cut(s) 122, 525, 938, 1639
AcuI CTGAAG 7 cut(s) 363, 687, 945, 1928, 1974, 2565, 2772
AdeI CACNNNGTG 1 cut(s) 1689
AfeI AGCGCT 1 cut(s) 2526
AfiI CCNNNNNNNGG 4 cut(s) 365, 1064, 2349, 2350
AflII CTTAAG 1 cut(s) 2060
AflIII ACRYGT 2 cut(s) 416, 442
AhdI GACNNNNNGTC 1 cut(s) 2328
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 6 cut(s) 222, 972, 1119, 1188, 1288, 2156
AloI GAACNNNNNNTCC 4 cut(s) 273, 305, 1095, 1127
Alw26I GTCTC 4 cut(s) 117, 2181, 2364, 2618
AlwI GGATC 5 cut(s) 1213, 1862, 2206, 2735, 2748
AlwNI CAGNNNCTG 2 cut(s) 239, 1793
Ama87I CYCGRG 2 cut(s) 401, 1592
Aor13HI TCCGGA 1 cut(s) 2448
Aor51HI AGCGCT 1 cut(s) 2526
AoxI GGCC 4 cut(s) 46, 1031, 1117, 2340
ApaI GGGCCC 1 cut(s) 2344
ApoI RAATTY 4 cut(s) 122, 525, 938, 1639
ArsI GACNNNNNNTTYG 3 cut(s) 432, 464, 2859
AseI ATTAAT 1 cut(s) 1425
Asp700I GAANNNNTTC 4 cut(s) 125, 175, 895, 2828
AspLEI GCGC 3 cut(s) 75, 920, 2527
AspS9I GGNCC 6 cut(s) 1589, 2280, 2310, 2340, 2341, 2446
AsuC2I CCSGG 2 cut(s) 58, 133
AsuHPI GGTGA 3 cut(s) 121, 1679, 2146
AvaI CYCGRG 2 cut(s) 401, 1592
AvaII GGWCC 4 cut(s) 1589, 2280, 2310, 2446
BaeGI GKGCMC 1 cut(s) 2344
BalI TGGCCA 1 cut(s) 48
BamHI GGATCC 1 cut(s) 2740
BanII GRGCYC 2 cut(s) 1059, 2344
BbsI GAAGAC 4 cut(s) 501, 1302, 1356, 2667
BccI CCATC 8 cut(s) 608, 638, 649, 749, 979, 1166, 1378, 1850
BceAI ACGGC 2 cut(s) 230, 782
BcgI CGANNNNNNTGC 6 cut(s) 427, 461, 728, 762, 2595, 2629
BciT130I CCWGG 6 cut(s) 224, 974, 1121, 1190, 1290, 2158
BclI TGATCA 1 cut(s) 1458
BcnI CCSGG 2 cut(s) 58, 133
BcoDI GTCTC 4 cut(s) 117, 2181, 2364, 2618
BfaI CTAG 6 cut(s) 1313, 2078, 2087, 2240, 2568, 2766
BfmI CTRYAG 5 cut(s) 240, 930, 1252, 1914, 2508
BfoI RGCGCY 1 cut(s) 2528
BfrI CTTAAG 1 cut(s) 2060
BfuAI ACCTGC 2 cut(s) 224, 2268
BglI GCCNNNNNGGC 1 cut(s) 803
BglII AGATCT 1 cut(s) 1920
BlpI GCTNAGC 1 cut(s) 1053
BmcAI AGTACT 2 cut(s) 895, 1280
Bme1390I CCNGG 8 cut(s) 58, 133, 224, 974, 1121, 1190, 1290, 2158
Bme18I GGWCC 4 cut(s) 1589, 2280, 2310, 2446
BmeRI GACNNNNNGTC 1 cut(s) 2328
BmeT110I CYCGRG 2 cut(s) 401, 1592
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 6 cut(s) 1589, 2280, 2310, 2340, 2341, 2446
BmiI GGNNCC 5 cut(s) 465, 544, 1591, 2342, 2742
BmrFI CCNGG 8 cut(s) 58, 133, 224, 974, 1121, 1190, 1290, 2158
BmrI ACTGGG 1 cut(s) 2568
BmsI GCATC 9 cut(s) 84, 193, 418, 652, 758, 784, 1367, 1489, 2661
BmuI ACTGGG 1 cut(s) 2568
BpiI GAAGAC 4 cut(s) 501, 1302, 1356, 2667
BplI GAGNNNNNCTC 2 cut(s) 1855, 1887
BpmI CTGGAG 4 cut(s) 693, 1172, 1259, 2346
Bpu10I CCTNAGC 1 cut(s) 1413
Bpu1102I GCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 5 cut(s) 345, 599, 1920, 2016, 2538
BpuMI CCSGG 2 cut(s) 58, 133
BsaBI GATNNNNATC 2 cut(s) 24, 1872
BsaI GGTCTC 1 cut(s) 2364
BsaJI CCNNGG 6 cut(s) 132, 402, 1288, 1707, 2157, 2306
BsaWI WCCGGW 1 cut(s) 2448
BsaXI ACNNNNNCTCC 2 cut(s) 1095, 1125
Bsc4I CCNNNNNNNGG 4 cut(s) 365, 1064, 2349, 2350
Bse118I RCCGGY 2 cut(s) 1033, 1408
Bse1I ACTGG 3 cut(s) 206, 1276, 2574
Bse3DI GCAATG 1 cut(s) 2649
Bse8I GATNNNNATC 2 cut(s) 24, 1872
BseAI TCCGGA 1 cut(s) 2448
BseBI CCWGG 6 cut(s) 224, 974, 1121, 1190, 1290, 2158
BseDI CCNNGG 6 cut(s) 132, 402, 1288, 1707, 2157, 2306
BseGI GGATG 5 cut(s) 232, 649, 657, 1530, 1861
BseJI GATNNNNATC 2 cut(s) 24, 1872
BseLI CCNNNNNNNGG 4 cut(s) 365, 1064, 2349, 2350
BseMI GCAATG 1 cut(s) 2649
BseMII CTCAG 7 cut(s) 798, 1044, 1427, 1512, 1800, 1974, 2748
BseNI ACTGG 3 cut(s) 206, 1276, 2574
BseRI GAGGAG 1 cut(s) 419
BseSI GKGCMC 1 cut(s) 2344
Bsh1236I CGCG 2 cut(s) 418, 2346
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 4 cut(s) 48, 1033, 1119, 2342
BsiEI CGRYCG 1 cut(s) 479
BsiHKCI CYCGRG 2 cut(s) 401, 1592
BsiSI CCGG 5 cut(s) 57, 132, 1034, 1409, 2449
BslFI GGGAC 6 cut(s) 798, 900, 2016, 2174, 2246, 2367
BslI CCNNNNNNNGG 4 cut(s) 365, 1064, 2349, 2350
BsmAI GTCTC 4 cut(s) 117, 2181, 2364, 2618
BsmFI GGGAC 6 cut(s) 798, 900, 2016, 2174, 2246, 2367
BsmI GAATGC 1 cut(s) 2184
BsnI GGCC 4 cut(s) 48, 1033, 1119, 2342
Bso31I GGTCTC 1 cut(s) 2364
BsoBI CYCGRG 2 cut(s) 401, 1592
Bsp120I GGGCCC 1 cut(s) 2340
Bsp1286I GDGCHC 2 cut(s) 1059, 2344
Bsp13I TCCGGA 1 cut(s) 2448
Bsp1720I GCTNAGC 1 cut(s) 1053
BspANI GGCC 4 cut(s) 48, 1033, 1119, 2342
BspCNI CTCAG 7 cut(s) 799, 1045, 1426, 1513, 1799, 1975, 2747
BspEI TCCGGA 1 cut(s) 2448
BspFNI CGCG 2 cut(s) 418, 2346
BspHI TCATGA 2 cut(s) 187, 249
BspLI GGNNCC 5 cut(s) 465, 544, 1591, 2342, 2742
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 2 cut(s) 224, 2268
BspPI GGATC 5 cut(s) 1213, 1862, 2206, 2735, 2748
BspQI GCTCTTC 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 2060
BspTNI GGTCTC 1 cut(s) 2364
BsrDI GCAATG 1 cut(s) 2649
BsrFI RCCGGY 2 cut(s) 1033, 1408
BsrI ACTGG 3 cut(s) 206, 1276, 2574
BssAI RCCGGY 2 cut(s) 1033, 1408
BssECI CCNNGG 6 cut(s) 132, 402, 1288, 1707, 2157, 2306
Bst2UI CCWGG 6 cut(s) 224, 974, 1121, 1190, 1290, 2158
Bst6I CTCTTC 4 cut(s) 39, 2170, 2682, 2764
BstAFI CTTAAG 1 cut(s) 2060
BstC8I GCNNGC 8 cut(s) 46, 1035, 1334, 1410, 1420, 1605, 2208, 2344
BstENI CCTNNNNNAGG 1 cut(s) 363
BstF5I GGATG 5 cut(s) 232, 649, 657, 1530, 1861
BstFNI CGCG 2 cut(s) 418, 2346
BstH2I RGCGCY 1 cut(s) 2528
BstHHI GCGC 3 cut(s) 75, 920, 2527
BstMAI GTCTC 4 cut(s) 117, 2181, 2364, 2618
BstMCI CGRYCG 1 cut(s) 479
BstNI CCWGG 6 cut(s) 224, 974, 1121, 1190, 1290, 2158
BstNSI RCATGY 2 cut(s) 446, 1603
BstSCI CCNGG 8 cut(s) 56, 131, 222, 972, 1119, 1188, 1288, 2156
BstSFI CTRYAG 5 cut(s) 240, 930, 1252, 1914, 2508
BstSLI GKGCMC 1 cut(s) 2344
BstUI CGCG 2 cut(s) 418, 2346
BstV2I GAAGAC 4 cut(s) 501, 1302, 1356, 2667
BstX2I RGATCY 3 cut(s) 1218, 1920, 2740
BstYI RGATCY 3 cut(s) 1218, 1920, 2740
BsuRI GGCC 4 cut(s) 48, 1033, 1119, 2342
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 5 cut(s) 232, 649, 657, 1530, 1861
BtsIMutI CAGTG 8 cut(s) 96, 544, 778, 985, 1008, 1565, 1648, 1980
BveI ACCTGC 2 cut(s) 224, 2268
Cac8I GCNNGC 8 cut(s) 46, 1035, 1334, 1410, 1420, 1605, 2208, 2344
CaiI CAGNNNCTG 2 cut(s) 239, 1793
CciI TCATGA 2 cut(s) 187, 249
CfoI GCGC 3 cut(s) 75, 920, 2527
Cfr10I RCCGGY 2 cut(s) 1033, 1408
Cfr13I GGNCC 6 cut(s) 1589, 2280, 2310, 2340, 2341, 2446
CseI GACGC 3 cut(s) 153, 407, 424
CsiI ACCWGGT 1 cut(s) 972
DraIII CACNNNGTG 1 cut(s) 1689
DrdI GACNNNNNNGTC 1 cut(s) 163
DriI GACNNNNNGTC 1 cut(s) 2328
DseDI GACNNNNNNGTC 1 cut(s) 163
EaeI YGGCCR 2 cut(s) 46, 1031
Eam1104I CTCTTC 4 cut(s) 39, 2170, 2682, 2764
Eam1105I GACNNNNNGTC 1 cut(s) 2328
EarI CTCTTC 4 cut(s) 39, 2170, 2682, 2764
Eco147I AGGCCT 1 cut(s) 1119
Eco24I GRGCYC 2 cut(s) 1059, 2344
Eco31I GGTCTC 1 cut(s) 2364
Eco32I GATATC 2 cut(s) 628, 1960
Eco47I GGWCC 4 cut(s) 1589, 2280, 2310, 2446
Eco47III AGCGCT 1 cut(s) 2526
Eco57I CTGAAG 7 cut(s) 363, 687, 945, 1928, 1974, 2565, 2772
Eco88I CYCGRG 2 cut(s) 401, 1592
EcoNI CCTNNNNNAGG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 2340
EcoRII CCWGG 6 cut(s) 222, 972, 1119, 1188, 1288, 2156
EcoRV GATATC 2 cut(s) 628, 1960
EcoT22I ATGCAT 1 cut(s) 2184
EcoT38I GRGCYC 2 cut(s) 1059, 2344
FalI AAGNNNNNCTT 6 cut(s) 163, 195, 1486, 1518, 2308, 2340
FaqI GGGAC 6 cut(s) 798, 900, 2016, 2174, 2246, 2367
FauI CCCGC 2 cut(s) 1250, 2351
FauNDI CATATG 1 cut(s) 2713
FbaI TGATCA 1 cut(s) 1458
FblI GTMKAC 1 cut(s) 2757
FokI GGATG 5 cut(s) 239, 656, 664, 1517, 1868
FriOI GRGCYC 2 cut(s) 1059, 2344
FspBI CTAG 6 cut(s) 1313, 2078, 2087, 2240, 2568, 2766
GlaI GCGC 3 cut(s) 74, 919, 2526
GsuI CTGGAG 4 cut(s) 693, 1172, 1259, 2346
HaeII RGCGCY 1 cut(s) 2528
HaeIII GGCC 4 cut(s) 48, 1033, 1119, 2342
HapII CCGG 5 cut(s) 57, 132, 1034, 1409, 2449
HgaI GACGC 3 cut(s) 153, 407, 424
HhaI GCGC 3 cut(s) 75, 920, 2527
Hin6I GCGC 3 cut(s) 73, 918, 2525
HinP1I GCGC 3 cut(s) 73, 918, 2525
HincII GTYRAC 3 cut(s) 1108, 2014, 2758
HindII GTYRAC 3 cut(s) 1108, 2014, 2758
HindIII AAGCTT 4 cut(s) 1468, 2034, 2204, 2453
HpaI GTTAAC 1 cut(s) 1108
HpaII CCGG 5 cut(s) 57, 132, 1034, 1409, 2449
HphI GGTGA 3 cut(s) 121, 1679, 2146
Hpy166II GTNNAC 5 cut(s) 281, 1108, 2014, 2283, 2758
Hpy8I GTNNAC 5 cut(s) 281, 1108, 2014, 2283, 2758
HpyAV CCTTC 8 cut(s) 359, 775, 877, 983, 1232, 1550, 1775, 1903
HpyCH4IV ACGT 4 cut(s) 5, 283, 498, 1110
HpySE526I ACGT 4 cut(s) 5, 283, 498, 1110
HspAI GCGC 3 cut(s) 73, 918, 2525
Kpn2I TCCGGA 1 cut(s) 2448
KroI GCCGGC 2 cut(s) 1033, 1408
KroNI GCCGGC 2 cut(s) 1035, 1410
Ksp22I TGATCA 1 cut(s) 1458
KspAI GTTAAC 1 cut(s) 1108
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 4 cut(s) 41, 406, 709, 2500
LweI GCATC 9 cut(s) 84, 193, 418, 652, 758, 784, 1367, 1489, 2661
MabI ACCWGGT 1 cut(s) 972
MaeI CTAG 6 cut(s) 1313, 2078, 2087, 2240, 2568, 2766
MaeII ACGT 4 cut(s) 5, 283, 498, 1110
MaeIII GTNAC 4 cut(s) 94, 311, 1976, 2113
MfeI CAATTG 1 cut(s) 1204
MflI RGATCY 3 cut(s) 1218, 1920, 2740
MhlI GDGCHC 2 cut(s) 1059, 2344
MlsI TGGCCA 1 cut(s) 48
MluI ACGCGT 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 9 cut(s) 119, 475, 527, 1799, 2156, 2183, 2268, 2657, 2726
MmeI TCCRAC 1 cut(s) 1719
Mox20I TGGCCA 1 cut(s) 48
Mph1103I ATGCAT 1 cut(s) 2184
MroI TCCGGA 1 cut(s) 2448
MroNI GCCGGC 2 cut(s) 1033, 1408
MroXI GAANNNNTTC 4 cut(s) 125, 175, 895, 2828
MscI TGGCCA 1 cut(s) 48
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 3 cut(s) 635, 746, 1096
MspCI CTTAAG 1 cut(s) 2060
MspI CCGG 5 cut(s) 57, 132, 1034, 1409, 2449
MspR9I CCNGG 8 cut(s) 58, 133, 224, 974, 1121, 1190, 1290, 2158
MunI CAATTG 1 cut(s) 1204
Mva1269I GAATGC 1 cut(s) 2184
MvaI CCWGG 6 cut(s) 224, 974, 1121, 1190, 1290, 2158
MvnI CGCG 2 cut(s) 418, 2346
NaeI GCCGGC 2 cut(s) 1035, 1410
NciI CCSGG 2 cut(s) 58, 133
NdeI CATATG 1 cut(s) 2713
NgoMIV GCCGGC 2 cut(s) 1033, 1408
NlaIV GGNNCC 5 cut(s) 465, 544, 1591, 2342, 2742
NmuCI GTSAC 3 cut(s) 94, 311, 1976
NsiI ATGCAT 1 cut(s) 2184
NspI RCATGY 2 cut(s) 446, 1603
PagI TCATGA 2 cut(s) 187, 249
PaqCI CACCTGC 1 cut(s) 224
PceI AGGCCT 1 cut(s) 1119
PciI ACATGT 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 39
PctI GAATGC 1 cut(s) 2184
PdiI GCCGGC 2 cut(s) 1035, 1410
PdmI GAANNNNTTC 4 cut(s) 125, 175, 895, 2828
PfeI GAWTC 8 cut(s) 137, 215, 266, 1025, 1200, 1391, 1670, 2083
PfoI TCCNGGA 1 cut(s) 56
Ple19I CGATCG 1 cut(s) 479
PleI GAGTC 9 cut(s) 118, 475, 526, 1798, 2156, 2183, 2267, 2657, 2726
PpsI GAGTC 9 cut(s) 118, 475, 526, 1798, 2156, 2183, 2267, 2657, 2726
PscI ACATGT 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 1425
Psp1406I AACGTT 1 cut(s) 1110
Psp6I CCWGG 6 cut(s) 222, 972, 1119, 1188, 1288, 2156
PspGI CCWGG 6 cut(s) 222, 972, 1119, 1188, 1288, 2156
PspN4I GGNNCC 5 cut(s) 465, 544, 1591, 2342, 2742
PspOMI GGGCCC 1 cut(s) 2340
PspPI GGNCC 6 cut(s) 1589, 2280, 2310, 2340, 2341, 2446
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 2 cut(s) 239, 1793
PsuI RGATCY 3 cut(s) 1218, 1920, 2740
PvuI CGATCG 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 635
SalI GTCGAC 1 cut(s) 2756
SapI GCTCTTC 1 cut(s) 39
Sau96I GGNCC 6 cut(s) 1589, 2280, 2310, 2340, 2341, 2446
ScaI AGTACT 2 cut(s) 895, 1280
SchI GAGTC 9 cut(s) 119, 475, 527, 1799, 2156, 2183, 2268, 2657, 2726
ScrFI CCNGG 8 cut(s) 58, 133, 224, 974, 1121, 1190, 1290, 2158
SduI GDGCHC 2 cut(s) 1059, 2344
SexAI ACCWGGT 1 cut(s) 972
SfaNI GCATC 9 cut(s) 84, 193, 418, 652, 758, 784, 1367, 1489, 2661
SfcI CTRYAG 5 cut(s) 240, 930, 1252, 1914, 2508
SinI GGWCC 4 cut(s) 1589, 2280, 2310, 2446
SmlI CTYRAG 6 cut(s) 360, 578, 1935, 2031, 2060, 2553
SmoI CTYRAG 6 cut(s) 360, 578, 1935, 2031, 2060, 2553
SseBI AGGCCT 1 cut(s) 1119
SspMI CTAG 6 cut(s) 1313, 2078, 2087, 2240, 2568, 2766
StuI AGGCCT 1 cut(s) 1119
StyD4I CCNGG 8 cut(s) 56, 131, 222, 972, 1119, 1188, 1288, 2156
TaiI ACGT 4 cut(s) 8, 286, 501, 1113
TaqII GACCGA 1 cut(s) 2298
TatI WGTACW 5 cut(s) 304, 591, 893, 1278, 1512
TauI GCSGC 3 cut(s) 1099, 1625, 2236
TfiI GAWTC 8 cut(s) 137, 215, 266, 1025, 1200, 1391, 1670, 2083
TscAI CASTG 8 cut(s) 103, 544, 778, 985, 1008, 1572, 1655, 1987
TseFI GTSAC 3 cut(s) 94, 311, 1976
Tsp45I GTSAC 3 cut(s) 94, 311, 1976
TspDTI ATGAA 9 cut(s) 243, 1011, 1017, 1412, 1462, 1773, 1954, 2673, 2730
TspGWI ACGGA 3 cut(s) 440, 456, 935
TspRI CASTG 8 cut(s) 103, 544, 778, 985, 1008, 1572, 1655, 1987
Vha464I CTTAAG 1 cut(s) 2060
VpaK11BI GGWCC 4 cut(s) 1589, 2280, 2310, 2446
VspI ATTAAT 1 cut(s) 1425
XagI CCTNNNNNAGG 1 cut(s) 363
XapI RAATTY 4 cut(s) 122, 525, 938, 1639
XbaI TCTAGA 1 cut(s) 2239
XceI RCATGY 2 cut(s) 446, 1603
XmiI GTMKAC 1 cut(s) 2757
XmnI GAANNNNTTC 4 cut(s) 125, 175, 895, 2828
XspI CTAG 6 cut(s) 1313, 2078, 2087, 2240, 2568, 2766
ZrmI AGTACT 2 cut(s) 895, 1280
Zsp2I ATGCAT 1 cut(s) 2184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.