Rroxscaffold_5G00345610

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001569
Physical Location & Seq
Forward (+)
16042289 .. 16052198
9910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345610.1

Sequence Viewer

Length: 2862 bp
ATGGCTGTTTCCATGAGAGATTTGGATCCAGCTTTCCACGGAGCTGGGCAAAAGGCTGGACTCGAAATATGGCGCATTGAAAACTTTCATCCTGTTATTGTCCCACAATCCTCTCATGGAAAGTTTTTTATGGGGGACTCTTATGTGATCTTGAAAACAACAGCCTCAAAAAGTGGTGCCTTGCGACATGATATCCATTATTGGCTTGGTAAAGATACTTCTCAGGATGAAGCAGGCACTGCAGCCATTAAGACAGTTGAATTAGATGCAGCTCTTGGAGGACGTGCTGTTCAGTATCGTGAAGTACAGGGTCATGAAACTGCTAAATTTCTATCTTATTTCAAACCGTGTATCATACCTCAAGAAGGTGGAATTGCTTCTGGGTTTAAACATGCTGAGGCTGAAGAACATACAACGCGGTTGTTTGTTTGCAAAGGAAAACATGTCGTCCATGTCAAAGAGGTTTCTTTTGCTCGATCTTCACTCAACCACGACGACATTTTTGTTCTGGATACCAAGTCTAAAATTTTTCAGTTTAATGGTTCCAACTCATCCATTCAAGAAAGGGCAAAGGCTTTAGAAGTTGTCCAGTACATTAAAGACACGTATCACGACGGGAAATGTGACATAGCTTCCATTGAGGATGGAAAGTTGATGGCTGATGCTGATACTGGAGAATTCTGGGCTTTATTTGGTGGTTTTGCTCCACTTCCGAAGAAAACAGCGAATGATGAAGACAAAAATTTTGATTCTCATAGTACTAAGCTACTACGCGTTGAGAAAGAGAAGGCAGAACCAGTTGAGGCTGATTCTTTGACAAGGGAATTACTAGAAACAAATAAGTGCTATCTTCTAGATTGTGGGCTGGAGTTGTTTGTTTGGATGGGGAGAAACACATCACTTGATGAAAGGAGGAGTGCAAGTGGAGCTGCAGAAGAGTTGCTACATGGTCCTGATCGGTCTAAATCCCATATGATTCGTGTTATTGAGGGATTTGAGACGGTAATGTTCAAGTCCAAATTTGATTCATGGCCTCAAACAGTTGAAGTAGCTGTGTCAGAGGATGGTAGGGGCAAGGTTGCAGCACTTCTAAAGCGTCAAGGGGTTAATGTGAAGGGTCTGTTGAAAGCTGATCCTGTAAAAGAAGAACCTCAACCATACATCGATTGCACAGGAAATCTACTGGTTTGGCGAGTGAATGGCCAGGAAAAGATTCTTCTTCCTGCTTCTGATCAGTCAAAAATTTATAGTGGAGATTGCTATATCTTCCAGTACTCGTATCCAGGAGAAGATAAAGAAGAACAGCTTATAGGAACATGGTTTGGAAAGCAGAGTATTGAGGAAGAAAGAGCATCAGCTATTTCACTAGCAAGCAAGATGGTTGAGTCATTGAAGTTTCTGCCAGCCCAGGCACGTATCTATGAAGGAAATGAACCAATTCAGTTCTATTCAATCTTCCAAAGCTTAATTGTTCTGAAGGGTGGTCTAAGTGATGGATATAAAAAGTACGTTGCGGAGAAGGAAGTTCCAGATGAGACATATCAAGAAGATGGTGTTGCTTTATTTCGTGTCCAGGGATCTGGACCTGATAATATGCAAGCAATTCAAGTTGAAGCAGTTGCATCATCTTTGAATTCCTCCTACTGTTATATATTACACAGTGGCTCAACTGTCTTTACATGGTCTGGAAGCCTTGCAACCACAGATGATCAGGAACTTGTTGAGAGGCAGCTGGATCTAATAAAGCCAAATCTTCAGTCGAAACCACAAAAGGAAAATACCGAATCTGAACAGTTTTGGGATTTGTTAGGAGGAAAGGCCGAGTATCCCAGCCAAAAAATTGTAAGAGATGCAGAAAGTGATCCTCGCCTATTTTCTTGCATCTTCTCAAATGAGAATTTAAAGGTAGTGGAGATATACAATTTCACCCAGGACGATTTGATGACTGAAGATATGTTCATCTTGGATTGTCACACTGATATCTTTGTTTGGGTTGGCCAAGAAGTCAACTCCAAGGATAAAATGCATGCTTTAACTATTGGGGAGAAATTTCTTGAGCGTGATTTTCTTATGGAAAAATTATCTCGTCAAGCTCCAATATATATTGTCATGGAAGGAAGTGAGCCACCTTTCTTCACACGCTTCTTCACTTGGGACTCTGCAAAATCTAAGATGCATGGAAATTCATTCCAACGAAAACTTACTATAGTGAAAGATGGGCGTGCTCCAGTTGTAGATAAACCCAAACGAAGAACACCTGTATCTTATGGTGGGAGGTCTAGTGTGCCAGAAAAATCACAGCGTTCCAGAAGCATGTCTTTCAGTCCTGACCGAGTGCGTGTGAGGGGCAGGTCTCCAGCTTTCAATGCACTGGCTGCTACTTTTGAGAGTGCTAATGCGAGGAACCTTTCAACTCCACCCCCAATGGTCAGAAAATCCCAACTGTATCCAAAATCCGTAACCCCAGATTCATCAAAATTGGCTTCAAAGTCATCAGCTATAGCAGCACTCACTGCTAGCTTTGAAAAAACAAATAATATACCAAGATCACCTAAAGGGGCTCCGAAACCAAAACCAGAGACAATTAGCAAGGACACTTTTATGAGCAGCAAAATGGAATCCCTCACGATAGAGGAAGATGTGAAAGAGGGTGAAGCAGAAGATGAAGGTGTTCCCATTTACCCATATGAACGCCTTAAAACAACGTCAACGGATCCTGTTGCAGAAATTGATGTGACGAAGCGAGAGATTTACTTGTCATCAGAGGAGTTCAGGGAGCATCTTGGGATGGCAAAGGATGCTTTTTATAAGTTGCCCAAGTGGAAACAGAACAAGCTTAAAATGGCCGTTCAACTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

953

Amino Acids

106.94

Weight (kDa)

5.87

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 31 - 111 9e-14 Gelsolin repeat
Gelsolin PF00626 149 - 208 7.2e-10 Gelsolin repeat
Gelsolin PF00626 265 - 317 1.8e-09 Gelsolin repeat
Gelsolin PF00626 635 - 712 1.2e-08 Gelsolin repeat
VHP PF02209 918 - 953 4.4e-15 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2808
Acc36I ACCTGC 1 cut(s) 2340
AccB1I GGYRCC 1 cut(s) 176
AccII CGCG 2 cut(s) 418, 774
AciI CCGC 2 cut(s) 418, 1514
AclWI GGATC 8 cut(s) 20, 33, 1127, 1585, 1743, 1856, 2708, 2721
AcoI YGGCCR 3 cut(s) 1201, 1996, 2844
AcuI CTGAAG 4 cut(s) 423, 1496, 1739, 1968
AfaI GTAC 5 cut(s) 306, 593, 760, 1274, 1508
AfiI CCNNNNNNNGG 1 cut(s) 365
AflIII ACRYGT 3 cut(s) 442, 603, 772
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 5 cut(s) 1203, 1282, 1407, 1572, 1929
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
Alw21I GWGCWC 1 cut(s) 2227
Alw26I GTCTC 4 cut(s) 992, 1529, 2358, 2573
AlwI GGATC 8 cut(s) 20, 33, 1127, 1585, 1743, 1856, 2708, 2721
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 5 cut(s) 1031, 1201, 1818, 1996, 2844
ApeKI GCWGC 8 cut(s) 242, 269, 929, 1082, 1729, 2375, 2504, 2607
ArsI GACNNNNNNTTYG 6 cut(s) 485, 517, 728, 760, 1742, 1774
Asp700I GAANNNNTTC 5 cut(s) 84, 376, 1212, 1437, 2670
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 950, 1583
AsuHPI GGTGA 3 cut(s) 1918, 2541, 2663
AsuNHI GCTAGC 1 cut(s) 2516
AvaII GGWCC 2 cut(s) 950, 1583
BaeI ACNNNNGTAYC 2 cut(s) 2244, 2277
BalI TGGCCA 2 cut(s) 1203, 1998
BamHI GGATCC 2 cut(s) 25, 2713
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 2563
BarI GAAGNNNNNNTAC 4 cut(s) 202, 234, 927, 959
BbsI GAAGAC 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 2227
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2619
BccI CCATC 9 cut(s) 638, 649, 877, 1058, 1372, 1487, 1544, 2210, 2782
BceAI ACGGC 1 cut(s) 2831
BciT130I CCWGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
BciVI GTATCC 4 cut(s) 505, 1290, 1836, 2457
BclI TGATCA 2 cut(s) 1231, 1708
BcoDI GTCTC 4 cut(s) 992, 1529, 2358, 2573
BfaI CTAG 5 cut(s) 830, 854, 1367, 2280, 2517
BfmI CTRYAG 4 cut(s) 240, 930, 2205, 2499
BfuAI ACCTGC 1 cut(s) 2340
BfuI GTATCC 4 cut(s) 505, 1290, 1836, 2457
BisI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2608
BlsI GCNGC 8 cut(s) 244, 271, 931, 1084, 1731, 2377, 2506, 2609
BmcAI AGTACT 2 cut(s) 760, 1274
Bme1390I CCNGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
Bme18I GGWCC 2 cut(s) 950, 1583
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 950, 1583
BmiI GGNNCC 6 cut(s) 27, 178, 544, 2405, 2562, 2715
BmrFI CCNGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
BmsI GCATC 9 cut(s) 256, 652, 1361, 1631, 1840, 1890, 2163, 2788, 2788
BmtI GCTAGC 1 cut(s) 2520
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 4 cut(s) 693, 887, 2211, 2340
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 345, 2075
Bsa29I ATCGAT 1 cut(s) 1164
BsaAI YACGTR 2 cut(s) 606, 1415
BsaBI GATNNNNATC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 2358
BsaJI CCNNGG 5 cut(s) 37, 1407, 1573, 1929, 2013
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 2266, 2296
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse1I ACTGG 7 cut(s) 589, 676, 797, 1188, 1270, 2228, 2376
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
BseCI ATCGAT 1 cut(s) 1164
BseDI CCNNGG 5 cut(s) 37, 1407, 1573, 1929, 2013
BseGI GGATG 8 cut(s) 88, 232, 551, 649, 888, 1069, 2793, 2803
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMII CTCAG 2 cut(s) 236, 387
BseNI ACTGG 7 cut(s) 589, 676, 797, 1188, 1270, 2228, 2376
BseRI GAGGAG 2 cut(s) 928, 2780
BseXI GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2619
BseYI CCCAGC 2 cut(s) 44, 1829
Bsh1236I CGCG 2 cut(s) 418, 774
BshFI GGCC 5 cut(s) 1033, 1203, 1820, 1998, 2846
BshNI GGYRCC 1 cut(s) 176
BshVI ATCGAT 1 cut(s) 1164
BsiHKAI GWGCWC 1 cut(s) 2227
BslFI GGGAC 3 cut(s) 86, 149, 2168
BslI CCNNNNNNNGG 1 cut(s) 365
BsmAI GTCTC 4 cut(s) 992, 1529, 2358, 2573
BsmBI CGTCTC 1 cut(s) 992
BsmFI GGGAC 3 cut(s) 86, 149, 2168
BsnI GGCC 5 cut(s) 1033, 1203, 1820, 1998, 2846
Bso31I GGTCTC 1 cut(s) 2358
Bsp1286I GDGCHC 2 cut(s) 2227, 2563
BspACI CCGC 2 cut(s) 418, 1514
BspANI GGCC 5 cut(s) 1033, 1203, 1820, 1998, 2846
BspCNI CTCAG 2 cut(s) 235, 388
BspDI ATCGAT 1 cut(s) 1164
BspFNI CGCG 2 cut(s) 418, 774
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 6 cut(s) 27, 178, 544, 2405, 2562, 2715
BspMAI CTGCAG 2 cut(s) 244, 934
BspMI ACCTGC 1 cut(s) 2340
BspOI GCTAGC 1 cut(s) 2520
BspPI GGATC 8 cut(s) 20, 33, 1127, 1585, 1743, 1856, 2708, 2721
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 2358
BsrI ACTGG 7 cut(s) 589, 676, 797, 1188, 1270, 2228, 2376
BssECI CCNNGG 5 cut(s) 37, 1407, 1573, 1929, 2013
BssT1I CCWWGG 1 cut(s) 2013
Bst2UI CCWGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
Bst6I CTCTTC 1 cut(s) 930
BstAPI GCANNNNNTGC 4 cut(s) 239, 2375, 2513, 2798
BstBAI YACGTR 2 cut(s) 606, 1415
BstC8I GCNNGC 7 cut(s) 235, 1372, 1404, 1599, 2028, 2223, 2518
BstDEI CTNAG 5 cut(s) 222, 396, 762, 1487, 2169
BstDSI CCRYGG 1 cut(s) 37
BstENI CCTNNNNNAGG 1 cut(s) 363
BstF5I GGATG 8 cut(s) 88, 232, 551, 649, 888, 1069, 2793, 2803
BstFNI CGCG 2 cut(s) 418, 774
BstHHI GCGC 1 cut(s) 75
BstMAI GTCTC 4 cut(s) 992, 1529, 2358, 2573
BstMWI GCNNNNNNNGC 7 cut(s) 239, 926, 2366, 2375, 2504, 2513, 2798
BstNI CCWGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
BstNSI RCATGY 4 cut(s) 395, 446, 2030, 2317
BstSCI CCNGG 5 cut(s) 1203, 1282, 1407, 1572, 1929
BstSFI CTRYAG 4 cut(s) 240, 930, 2205, 2499
BstUI CGCG 2 cut(s) 418, 774
BstV1I GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2619
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 4 cut(s) 25, 1577, 1735, 2713
BstXI CCANNNNNNTGG 2 cut(s) 44, 1580
BstYI RGATCY 4 cut(s) 25, 1577, 1735, 2713
Bsu15I ATCGAT 1 cut(s) 1164
BsuI GTATCC 4 cut(s) 505, 1290, 1836, 2457
BsuRI GGCC 5 cut(s) 1033, 1203, 1820, 1998, 2846
BsuTUI ATCGAT 1 cut(s) 1164
BtgI CCRYGG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 8 cut(s) 88, 232, 551, 649, 888, 1069, 2793, 2803
BtsI GCAGTG 2 cut(s) 237, 2511
BtsIMutI CAGTG 5 cut(s) 237, 1666, 1974, 2369, 2511
BveI ACCTGC 1 cut(s) 2340
Cac8I GCNNGC 7 cut(s) 235, 1372, 1404, 1599, 2028, 2223, 2518
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 950, 1583
ClaI ATCGAT 1 cut(s) 1164
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 5 cut(s) 305, 592, 759, 1273, 1507
CviQI GTAC 5 cut(s) 305, 592, 759, 1273, 1507
DdeI CTNAG 5 cut(s) 222, 396, 762, 1487, 2169
DraI TTTAAA 2 cut(s) 388, 1902
EaeI YGGCCR 3 cut(s) 1201, 1996, 2844
Eam1104I CTCTTC 1 cut(s) 930
EarI CTCTTC 1 cut(s) 930
Eco130I CCWWGG 1 cut(s) 2013
Eco24I GRGCYC 1 cut(s) 2563
Eco31I GGTCTC 1 cut(s) 2358
Eco32I GATATC 2 cut(s) 193, 1981
Eco47I GGWCC 2 cut(s) 950, 1583
Eco57I CTGAAG 4 cut(s) 423, 1496, 1739, 1968
EcoNI CCTNNNNNAGG 1 cut(s) 363
EcoRI GAATTC 2 cut(s) 677, 1633
EcoRII CCWGG 5 cut(s) 1203, 1282, 1407, 1572, 1929
EcoRV GATATC 2 cut(s) 193, 1981
EcoT14I CCWWGG 1 cut(s) 2013
EcoT22I ATGCAT 2 cut(s) 2028, 2178
EcoT38I GRGCYC 1 cut(s) 2563
ErhI CCWWGG 1 cut(s) 2013
Esp3I CGTCTC 1 cut(s) 992
FalI AAGNNNNNCTT 4 cut(s) 1290, 1322, 2302, 2334
FaqI GGGAC 3 cut(s) 86, 149, 2168
FauNDI CATATG 2 cut(s) 972, 2686
FbaI TGATCA 2 cut(s) 1231, 1708
Fnu4HI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2608
FokI GGATG 8 cut(s) 75, 239, 538, 656, 895, 1076, 2800, 2810
FriOI GRGCYC 1 cut(s) 2563
Fsp4HI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2608
FspBI CTAG 5 cut(s) 830, 854, 1367, 2280, 2517
GlaI GCGC 1 cut(s) 74
GluI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2608
GsaI CCCAGC 2 cut(s) 48, 1833
GsuI CTGGAG 4 cut(s) 693, 887, 2211, 2340
HaeIII GGCC 5 cut(s) 1033, 1203, 1820, 1998, 2846
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 1 cut(s) 75
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 2 cut(s) 2008, 2709
HindII GTYRAC 2 cut(s) 2008, 2709
HindIII AAGCTT 2 cut(s) 1462, 2834
HphI GGTGA 3 cut(s) 1918, 2541, 2663
Hpy166II GTNNAC 2 cut(s) 2008, 2709
Hpy188I TCNGA 8 cut(s) 714, 1060, 1231, 1476, 1789, 2432, 2565, 2764
Hpy8I GTNNAC 2 cut(s) 2008, 2709
Hpy99I CGWCG 2 cut(s) 497, 617
HpyAV CCTTC 8 cut(s) 359, 781, 1108, 1418, 1471, 1513, 2108, 2660
HpyCH4IV ACGT 5 cut(s) 283, 605, 1414, 1509, 2705
HpyF10VI GCNNNNNNNGC 7 cut(s) 239, 926, 2366, 2375, 2504, 2513, 2798
HpyF3I CTNAG 5 cut(s) 222, 396, 762, 1487, 2169
HpySE526I ACGT 5 cut(s) 283, 605, 1414, 1509, 2705
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 2 cut(s) 1231, 1708
LmnI GCTCC 7 cut(s) 41, 709, 926, 2098, 2230, 2566, 2776
Lsp1109I GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2619
LweI GCATC 9 cut(s) 256, 652, 1361, 1631, 1840, 1890, 2163, 2788, 2788
MaeI CTAG 5 cut(s) 830, 854, 1367, 2280, 2517
MaeII ACGT 5 cut(s) 283, 605, 1414, 1509, 2705
MaeIII GTNAC 4 cut(s) 623, 1970, 2458, 2734
MflI RGATCY 4 cut(s) 25, 1577, 1735, 2713
MhlI GDGCHC 2 cut(s) 2227, 2563
MlsI TGGCCA 2 cut(s) 1203, 1998
MluI ACGCGT 1 cut(s) 772
MluNI TGGCCA 2 cut(s) 1203, 1998
MlyI GAGTC 4 cut(s) 54, 131, 1394, 2150
MmeI TCCRAC 2 cut(s) 570, 2215
Mox20I TGGCCA 2 cut(s) 1203, 1998
Mph1103I ATGCAT 2 cut(s) 2028, 2178
MroXI GAANNNNTTC 5 cut(s) 84, 376, 1212, 1437, 2670
MscI TGGCCA 2 cut(s) 1203, 1998
MslI CAYNNNNRTG 1 cut(s) 2600
Msp20I TGGCCA 2 cut(s) 1203, 1998
MspA1I CMGCKG 1 cut(s) 1732
MspR9I CCNGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
MssI GTTTAAAC 1 cut(s) 388
MvaI CCWGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
MvnI CGCG 2 cut(s) 418, 774
MwoI GCNNNNNNNGC 7 cut(s) 239, 926, 2366, 2375, 2504, 2513, 2798
NdeI CATATG 2 cut(s) 972, 2686
NheI GCTAGC 1 cut(s) 2516
NlaIV GGNNCC 6 cut(s) 27, 178, 544, 2405, 2562, 2715
NmeAIII GCCGAG 1 cut(s) 1846
NmuCI GTSAC 3 cut(s) 623, 1970, 2734
NsiI ATGCAT 2 cut(s) 2028, 2178
NspI RCATGY 4 cut(s) 395, 446, 2030, 2317
PaeI GCATGC 1 cut(s) 2030
PagI TCATGA 1 cut(s) 313
PciI ACATGT 1 cut(s) 442
PdmI GAANNNNTTC 5 cut(s) 84, 376, 1212, 1437, 2670
PfeI GAWTC 8 cut(s) 749, 809, 976, 1025, 1213, 1784, 2468, 2618
PfoI TCCNGGA 1 cut(s) 1282
PkrI GCNGC 8 cut(s) 244, 271, 931, 1084, 1731, 2377, 2506, 2609
PleI GAGTC 4 cut(s) 54, 131, 1393, 2150
PmeI GTTTAAAC 1 cut(s) 388
PpsI GAGTC 4 cut(s) 54, 131, 1393, 2150
Ppu21I YACGTR 2 cut(s) 606, 1415
PscI ACATGT 1 cut(s) 442
PsiI TTATAA 1 cut(s) 2808
Psp6I CCWGG 5 cut(s) 1203, 1282, 1407, 1572, 1929
PspFI CCCAGC 2 cut(s) 44, 1829
PspGI CCWGG 5 cut(s) 1203, 1282, 1407, 1572, 1929
PspN4I GGNNCC 6 cut(s) 27, 178, 544, 2405, 2562, 2715
PspPI GGNCC 2 cut(s) 950, 1583
PstI CTGCAG 2 cut(s) 244, 934
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 4 cut(s) 25, 1577, 1735, 2713
PvuII CAGCTG 1 cut(s) 1732
RsaI GTAC 5 cut(s) 306, 593, 760, 1274, 1508
RsaNI GTAC 5 cut(s) 305, 592, 759, 1273, 1507
RseI CAYNNNNRTG 1 cut(s) 2600
SatI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2608
Sau96I GGNCC 2 cut(s) 950, 1583
ScaI AGTACT 2 cut(s) 760, 1274
SchI GAGTC 4 cut(s) 54, 131, 1394, 2150
ScrFI CCNGG 5 cut(s) 1205, 1284, 1409, 1574, 1931
SduI GDGCHC 2 cut(s) 2227, 2563
SfaNI GCATC 9 cut(s) 256, 652, 1361, 1631, 1840, 1890, 2163, 2788, 2788
SfcI CTRYAG 4 cut(s) 240, 930, 2205, 2499
SinI GGWCC 2 cut(s) 950, 1583
SmiMI CAYNNNNRTG 1 cut(s) 2600
SmlI CTYRAG 2 cut(s) 360, 2054
SmoI CTYRAG 2 cut(s) 360, 2054
SphI GCATGC 1 cut(s) 2030
SsiI CCGC 2 cut(s) 418, 1514
SspMI CTAG 5 cut(s) 830, 854, 1367, 2280, 2517
StyD4I CCNGG 5 cut(s) 1203, 1282, 1407, 1572, 1929
StyI CCWWGG 1 cut(s) 2013
TaiI ACGT 5 cut(s) 286, 608, 1417, 1512, 2708
TaqI TCGA 4 cut(s) 63, 475, 1164, 1760
TaqII GACCGA 2 cut(s) 948, 2346
TatI WGTACW 4 cut(s) 304, 591, 758, 1272
TfiI GAWTC 8 cut(s) 749, 809, 976, 1025, 1213, 1784, 2468, 2618
TscAI CASTG 5 cut(s) 244, 1666, 1981, 2376, 2518
TseFI GTSAC 3 cut(s) 623, 1970, 2734
TseI GCWGC 8 cut(s) 242, 269, 929, 1082, 1729, 2375, 2504, 2607
Tsp45I GTSAC 3 cut(s) 623, 1970, 2734
TspGWI ACGGA 3 cut(s) 54, 2446, 2726
TspRI CASTG 5 cut(s) 244, 1666, 1981, 2376, 2518
VpaK11BI GGWCC 2 cut(s) 950, 1583
XagI CCTNNNNNAGG 1 cut(s) 363
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 4 cut(s) 395, 446, 2030, 2317
XcmI CCANNNNNNNNNTGG 2 cut(s) 19, 203
XmnI GAANNNNTTC 5 cut(s) 84, 376, 1212, 1437, 2670
XspI CTAG 5 cut(s) 830, 854, 1367, 2280, 2517
ZrmI AGTACT 2 cut(s) 760, 1274
Zsp2I ATGCAT 2 cut(s) 2028, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.