Rorug04G0025800

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001569
Physical Location & Seq
Reverse (-)
3554566 .. 3562573
8008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0025800.1

Sequence Viewer

Length: 1119 bp
ATGGGAAGTAGATACGGTGAGTACACATATGAGAACCTGGAGAGGGAACCGTACTGGCCTTCTGAGAAGCTTCGTATTTCTGTAACGGGGGCTGGTGGTTTCATTGCTTCACACATTGCCAGGCGACTGAAGAGTGAGGGTCATTACATTATTGCTTCTGACTGGAAGAAAAATGAGCACATGACCGAGGACATGTTCTGTAACGAGTTCCATCTGGTTGATCTTAGAGTGATGGATAACTGTTTGAAGGTTACTGCTGGTGTTAACCATGTTTTCAACCTTGCTGCTGACATGGGAGGTATGGGGTTCATTCAGTCCAACCATTCCGTCATTATGTATAACAACACAATGATCAGCTTTAATATGCTCGAGGCAGCAAGGATCAATGGAGCTAAGAGGTTCTTTTATGCTTCTAGTGCTTGCATTTACCCTGAATTCAAACAGTTGGATACTAATGTGAGCTTGAAGGAGTCTGATGCTTGGCCTGCAGAGCCACAAGACGCCTATGGCTTGGAGAAGCTCATGACTGAAGAATTATGCAAGCACTTTAATAAGGATTTCAGTATTGAATGTCGGATTGGACGGTTTCACAACATTTATGGACCCTTCGGCACTTGGAAAGGTGGTAGAGAGAAGGCCCCTGCTGCTTTTTGTAGAAAAACCCTTACTTCCACTGATAGGTTTGAGATGTGGGGAGACGGGCTACAGACTCGGTCTTTCACCTTCATTGATGAATGTGTAGAAGGTGTCCTGAGATTGACAAAGTCTGACTTCCGGGATCCGGTAAATATTGGAAGTGATGAGATGGTTAGCATGAATGAGATGGCTGAAATGGTCCTAAGCATTGAGAACAAGAAGCTTCCCATCCACCATATTCCTGGCCCTGAGGGTGTTCGTGGTCGAAACTCAGACAACACTTTGATTAAGGAGAAGCTTGGATGGGCTCCTGCTATGAAATTGAAGGACGGGCTGAGGATAACATACTTTTGGATAAAGGAACAACTTGAGAAAGAGGAGGCTCAGGGAATTAAAGTGTCAGGTTATGGCACATCAAAAGTAGTAGGAACACAAGCACCAGTTCAACTCGGCTCTCTTCGAGCCGCTGATGGGAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

372

Amino Acids

42.11

Weight (kDa)

6.25

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 26 - 265 9.1e-43 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 28 - 326 1.5e-24 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 28 - 185 4.7e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1101
AclWI GGATC 3 cut(s) 389, 773, 786
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 2 cut(s) 149, 549
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 2 cut(s) 23, 53
AfiI CCNNNNNNNGG 4 cut(s) 43, 678, 781, 1107
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 7 cut(s) 247, 277, 439, 466, 569, 961, 1082
AjnI CCWGG 3 cut(s) 36, 119, 877
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AluBI AGCT 7 cut(s) 70, 357, 392, 462, 520, 859, 934
AluI AGCT 7 cut(s) 70, 357, 392, 462, 520, 859, 934
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 690
AlwI GGATC 3 cut(s) 389, 773, 786
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 4 cut(s) 56, 482, 636, 880
ApeKI GCWGC 3 cut(s) 284, 374, 644
ApoI RAATTY 1 cut(s) 434
AspS9I GGNCC 4 cut(s) 602, 637, 835, 881
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 2 cut(s) 29, 712
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 602, 835
AxyI CCTNAGG 1 cut(s) 885
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BamHI GGATCC 1 cut(s) 778
BanII GRGCYC 1 cut(s) 946
Bbv12I GWGCWC 1 cut(s) 180
BbvCI CCTCAGC 1 cut(s) 971
BbvI GCAGC 3 cut(s) 271, 386, 631
BccI CCATC 7 cut(s) 219, 226, 799, 817, 872, 933, 1100
BciT130I CCWGG 3 cut(s) 38, 121, 879
BciVI GTATCC 1 cut(s) 442
BclI TGATCA 1 cut(s) 351
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 1 cut(s) 690
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 2 cut(s) 486, 704
BfuI GTATCC 1 cut(s) 442
BisI GCNGC 4 cut(s) 285, 375, 645, 1101
BlsI GCNGC 4 cut(s) 286, 376, 646, 1102
Bme1390I CCNGG 4 cut(s) 38, 121, 776, 879
Bme18I GGWCC 2 cut(s) 602, 835
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 4 cut(s) 602, 637, 835, 881
BmiI GGNNCC 5 cut(s) 48, 604, 639, 780, 945
BmrFI CCNGG 4 cut(s) 38, 121, 776, 879
BmsI GCATC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 59
Bpu10I CCTNAGC 3 cut(s) 839, 971, 1020
BpuEI CTTGAG 1 cut(s) 1025
BpuMI CCSGG 1 cut(s) 776
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 781
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 678, 781, 1107
Bse1I ACTGG 3 cut(s) 59, 167, 1076
Bse21I CCTNAGG 1 cut(s) 885
Bse3DI GCAATG 2 cut(s) 102, 114
BseBI CCWGG 3 cut(s) 38, 121, 879
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 864, 944
BseLI CCNNNNNNNGG 4 cut(s) 43, 678, 781, 1107
BseMI GCAATG 2 cut(s) 102, 114
BseMII CTCAG 6 cut(s) 54, 743, 876, 921, 962, 1034
BseNI ACTGG 3 cut(s) 59, 167, 1076
BseRI GAGGAG 1 cut(s) 1028
BseXI GCAGC 3 cut(s) 271, 386, 631
BshFI GGCC 4 cut(s) 58, 484, 638, 882
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 2 cut(s) 775, 782
BslI CCNNNNNNNGG 4 cut(s) 43, 678, 781, 1107
BsmAI GTCTC 1 cut(s) 690
BsmBI CGTCTC 1 cut(s) 690
BsnI GGCC 4 cut(s) 58, 484, 638, 882
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 180, 946
Bsp143I GATC 4 cut(s) 220, 351, 381, 778
BspACI CCGC 1 cut(s) 1101
BspANI GGCC 4 cut(s) 58, 484, 638, 882
BspCNI CTCAG 6 cut(s) 55, 744, 877, 920, 963, 1033
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 5 cut(s) 48, 604, 639, 780, 945
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 3 cut(s) 389, 773, 786
BsrDI GCAATG 2 cut(s) 102, 114
BsrI ACTGG 3 cut(s) 59, 167, 1076
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 4 cut(s) 220, 351, 381, 778
BssNI GRCGYC 1 cut(s) 501
Bst2UI CCWGG 3 cut(s) 38, 121, 879
Bst4CI ACNGT 5 cut(s) 17, 51, 242, 444, 585
Bst6I CTCTTC 2 cut(s) 125, 1098
BstACI GRCGYC 1 cut(s) 501
BstC8I GCNNGC 3 cut(s) 421, 486, 542
BstDEI CTNAG 9 cut(s) 63, 224, 393, 752, 839, 885, 907, 971, 1020
BstF5I GGATG 2 cut(s) 864, 944
BstKTI GATC 4 cut(s) 223, 354, 384, 781
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 4 cut(s) 220, 351, 381, 778
BstMWI GCNNNNNNNGC 4 cut(s) 416, 485, 490, 644
BstNI CCWGG 3 cut(s) 38, 121, 879
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 4 cut(s) 36, 119, 774, 877
BstSFI CTRYAG 2 cut(s) 486, 704
BstV1I GCAGC 3 cut(s) 271, 386, 631
BstX2I RGATCY 1 cut(s) 778
BstXI CCANNNNNNTGG 1 cut(s) 878
BstYI RGATCY 1 cut(s) 778
Bsu36I CCTNAGG 1 cut(s) 885
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 4 cut(s) 58, 484, 638, 882
BtsCI GGATG 2 cut(s) 864, 944
BtsIMutI CAGTG 1 cut(s) 672
Cac8I GCNNGC 3 cut(s) 421, 486, 542
CciI TCATGA 1 cut(s) 522
Cfr13I GGNCC 4 cut(s) 602, 637, 835, 881
CseI GACGC 1 cut(s) 509
Csp6I GTAC 2 cut(s) 22, 52
CviAII CATG 6 cut(s) 181, 193, 269, 292, 523, 814
CviQI GTAC 2 cut(s) 22, 52
DdeI CTNAG 9 cut(s) 63, 224, 393, 752, 839, 885, 907, 971, 1020
DpnI GATC 4 cut(s) 222, 353, 383, 780
DpnII GATC 4 cut(s) 220, 351, 381, 778
Eam1104I CTCTTC 2 cut(s) 125, 1098
EarI CTCTTC 2 cut(s) 125, 1098
Eco24I GRGCYC 1 cut(s) 946
Eco47I GGWCC 2 cut(s) 602, 835
Eco57I CTGAAG 2 cut(s) 149, 549
Eco81I CCTNAGG 1 cut(s) 885
Eco88I CYCGRG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 434
EcoRII CCWGG 3 cut(s) 36, 119, 877
EcoT38I GRGCYC 1 cut(s) 946
Esp3I CGTCTC 1 cut(s) 690
FaeI CATG 6 cut(s) 184, 196, 272, 295, 526, 817
FalI AAGNNNNNCTT 4 cut(s) 386, 418, 755, 787
FatI CATG 6 cut(s) 180, 192, 268, 291, 522, 813
FauNDI CATATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 4 cut(s) 285, 375, 645, 1101
FokI GGATG 2 cut(s) 851, 951
FriOI GRGCYC 1 cut(s) 946
Fsp4HI GCNGC 4 cut(s) 285, 375, 645, 1101
FspBI CTAG 1 cut(s) 414
GluI GCNGC 4 cut(s) 285, 375, 645, 1101
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 4 cut(s) 58, 484, 638, 882
HapII CCGG 2 cut(s) 775, 782
HgaI GACGC 1 cut(s) 509
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 6 cut(s) 184, 196, 272, 295, 526, 817
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 3 cut(s) 68, 857, 932
HinfI GANTC 2 cut(s) 470, 709
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 2 cut(s) 775, 782
HphI GGTGA 2 cut(s) 29, 712
Hpy166II GTNNAC 2 cut(s) 24, 265
Hpy188I TCNGA 6 cut(s) 64, 160, 475, 576, 769, 910
Hpy188III TCNNGA 2 cut(s) 523, 751
Hpy8I GTNNAC 2 cut(s) 24, 265
HpyAV CCTTC 8 cut(s) 69, 241, 460, 616, 628, 733, 737, 955
HpyCH4III ACNGT 5 cut(s) 17, 51, 242, 444, 585
HpyCH4V TGCA 3 cut(s) 423, 488, 540
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 485, 490, 644
HpyF3I CTNAG 9 cut(s) 63, 224, 393, 752, 839, 885, 907, 971, 1020
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 6 cut(s) 184, 196, 272, 295, 526, 817
Ksp22I TGATCA 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 4 cut(s) 220, 351, 381, 778
LmnI GCTCC 2 cut(s) 389, 949
Lsp1109I GCAGC 3 cut(s) 271, 386, 631
LweI GCATC 1 cut(s) 466
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 3 cut(s) 82, 200, 250
MalI GATC 4 cut(s) 222, 353, 383, 780
MboI GATC 4 cut(s) 220, 351, 381, 778
MboII GAAGA 4 cut(s) 142, 178, 542, 1085
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 2 cut(s) 180, 946
MluCI AATT 4 cut(s) 434, 533, 956, 1026
MlyI GAGTC 2 cut(s) 479, 703
MmeI TCCRAC 3 cut(s) 342, 426, 554
MseI TTAA 5 cut(s) 264, 360, 549, 924, 1029
MspA1I CMGCKG 1 cut(s) 1103
MspI CCGG 2 cut(s) 775, 782
MspR9I CCNGG 4 cut(s) 38, 121, 776, 879
MvaI CCWGG 3 cut(s) 38, 121, 879
MwoI GCNNNNNNNGC 4 cut(s) 416, 485, 490, 644
NciI CCSGG 1 cut(s) 776
NdeI CATATG 1 cut(s) 28
NdeII GATC 4 cut(s) 220, 351, 381, 778
NlaIII CATG 6 cut(s) 184, 196, 272, 295, 526, 817
NlaIV GGNNCC 5 cut(s) 48, 604, 639, 780, 945
NmeAIII GCCGAG 1 cut(s) 1065
NspI RCATGY 1 cut(s) 196
PaeR7I CTCGAG 1 cut(s) 368
PagI TCATGA 1 cut(s) 522
PciI ACATGT 1 cut(s) 192
PflFI GACNNNGTC 2 cut(s) 712, 763
PfoI TCCNGGA 1 cut(s) 774
PkrI GCNGC 4 cut(s) 286, 376, 646, 1102
PleI GAGTC 2 cut(s) 478, 703
PpsI GAGTC 2 cut(s) 478, 703
PscI ACATGT 1 cut(s) 192
Psp6I CCWGG 3 cut(s) 36, 119, 877
PspGI CCWGG 3 cut(s) 36, 119, 877
PspN4I GGNNCC 5 cut(s) 48, 604, 639, 780, 945
PspPI GGNCC 4 cut(s) 602, 637, 835, 881
PspXI VCTCGAGB 1 cut(s) 368
PstI CTGCAG 1 cut(s) 490
PsuI RGATCY 1 cut(s) 778
PsyI GACNNNGTC 2 cut(s) 712, 763
RsaI GTAC 2 cut(s) 23, 53
RsaNI GTAC 2 cut(s) 22, 52
SaqAI TTAA 5 cut(s) 264, 360, 549, 924, 1029
SatI GCNGC 4 cut(s) 285, 375, 645, 1101
Sau3AI GATC 4 cut(s) 220, 351, 381, 778
Sau96I GGNCC 4 cut(s) 602, 637, 835, 881
SchI GAGTC 2 cut(s) 479, 703
ScrFI CCNGG 4 cut(s) 38, 121, 776, 879
SduI GDGCHC 2 cut(s) 180, 946
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 2 cut(s) 486, 704
Sfr274I CTCGAG 1 cut(s) 368
SinI GGWCC 2 cut(s) 602, 835
SlaI CTCGAG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 368, 1004
SmoI CTYRAG 2 cut(s) 368, 1004
Sse9I AATT 4 cut(s) 434, 533, 956, 1026
SsiI CCGC 1 cut(s) 1101
SspI AATATT 1 cut(s) 790
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 4 cut(s) 36, 119, 774, 877
TaaI ACNGT 5 cut(s) 17, 51, 242, 444, 585
TaqI TCGA 3 cut(s) 369, 901, 1096
TaqII GACCGA 2 cut(s) 200, 702
TasI AATT 4 cut(s) 434, 533, 956, 1026
TatI WGTACW 1 cut(s) 21
TauI GCSGC 1 cut(s) 1103
Tru1I TTAA 5 cut(s) 264, 360, 549, 924, 1029
Tru9I TTAA 5 cut(s) 264, 360, 549, 924, 1029
TscAI CASTG 1 cut(s) 679
TseI GCWGC 3 cut(s) 284, 374, 644
TspDTI ATGAA 6 cut(s) 91, 298, 715, 747, 830, 968
TspGWI ACGGA 1 cut(s) 316
TspRI CASTG 1 cut(s) 679
Tth111I GACNNNGTC 2 cut(s) 712, 763
VpaK11BI GGWCC 2 cut(s) 602, 835
XapI RAATTY 1 cut(s) 434
XceI RCATGY 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 875
XhoI CTCGAG 1 cut(s) 368
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.