pycom13g21710

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001569
Physical Location & Seq
Forward (+)
18653084 .. 18655315
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21710.8

Sequence Viewer

Length: 975 bp
ATGGCTGTTTCCATGAGAGACTTGGATCCAGCTTTCCAGGGAGCTGGACAAAAGGATGGACTTGAAATATGGCGCATTGAAAACTTTCGCCCTGCTCCTATCCCAAAGTCCTCTTTTGGCAAATTTTTTACAGGGGACTCTTATGTGATCCTGAAAACTACAGCCTCAAAAAGTGGGGCTTTGCGGCATGAGATTCATTATTGGCTTGGAAAGGATACGTCTCAGGATGAAGCTGGAGCTGCTGCCATCAAGACAGTTGAGTTAGATGCAACCCTTGGAGGACGTGCTGTCCAGTATCGTGAAGTACAGGGTCACGAAACTGCTAAGTTCCTATCTTATTTCAAACCATGTATCATACCTCAAGAAGGTGGAGTTGCATCTGGGTTCAAACATGCTGAGGCTGAAGAACACAAAACACGATTGTTTGTGTGCCAAGGAAAACATGTTGTCCATGTCAAAGAGGTTGCTTTTGCTCGATCTTCACTCAGCCATGATGACATTTTTATTTTGGATACCAAGTCTAAAATTTTTCAATTTAATGGTTCAAACTCATCTATTCAAGAAAGAGCTAAGGCGTTAGAAGTTGTCCAGTACATTAAAGACGCATATCATGATGGGAAATGTGAGATAGCTTCCATTGAGGATGGAAAGTTGATGGCTGATGCAGAAAGTGGAGAATTTTGGGGTTTATTTGGTGGTTTTGCTCCACTTCCAAGGAAAACAGCCACCAATGAGGACAAAAGTTTTGATTCTTATCCTACTAAGCTTCTTTGTGTTGAGAAGGGACAGGCAGAACCAGTTGAGGGTGATGCTTTGACGAGGGATTTGCTAGACACAAATAAGTGCTATCTTCTAGATTGTGGGTTGGAAGTGTTTGTTTGGATGGGGAGAAATACATCTCTTGACCAAAGAAGGAGTGCAAGTGGAGCTGCAGAAGTACTATCCGTCCTCCTGATCGATCGAAATCCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

325

Amino Acids

35.75

Weight (kDa)

5.77

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 184
AclWI GGATC 3 cut(s) 20, 33, 142
AcsI RAATTY 3 cut(s) 122, 525, 677
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 3 cut(s) 306, 593, 939
AfiI CCNNNNNNNGG 2 cut(s) 365, 803
AflIII ACRYGT 1 cut(s) 442
AgsI TTSAA 7 cut(s) 65, 80, 343, 388, 533, 546, 560
AhdI GACNNNNNGTC 1 cut(s) 287
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 8 cut(s) 32, 44, 233, 239, 569, 632, 766, 929
AluI AGCT 8 cut(s) 32, 44, 233, 239, 569, 632, 766, 929
Alw26I GTCTC 2 cut(s) 12, 225
AlwI GGATC 3 cut(s) 20, 33, 142
ApeKI GCWGC 3 cut(s) 239, 242, 929
ApoI RAATTY 3 cut(s) 122, 525, 677
ArsI GACNNNNNNTTYG 4 cut(s) 728, 760, 808, 840
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 818
BamHI GGATCC 1 cut(s) 25
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 3 cut(s) 226, 229, 916
BccI CCATC 6 cut(s) 50, 254, 608, 638, 649, 877
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BciT130I CCWGG 1 cut(s) 38
BciVI GTATCC 2 cut(s) 208, 505
BcoDI GTCTC 2 cut(s) 12, 225
BfaI CTAG 2 cut(s) 830, 854
BfmI CTRYAG 2 cut(s) 159, 930
BfuI GTATCC 2 cut(s) 208, 505
BisI GCNGC 4 cut(s) 185, 240, 243, 930
BlsI GCNGC 4 cut(s) 186, 241, 244, 931
BmcAI AGTACT 1 cut(s) 939
Bme1390I CCNGG 1 cut(s) 38
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 4 cut(s) 256, 386, 652, 799
BpmI CTGGAG 1 cut(s) 255
Bpu10I CCTNAGC 2 cut(s) 396, 570
BpuEI CTTGAG 1 cut(s) 345
Bsa29I ATCGAT 1 cut(s) 957
BsaBI GATNNNNATC 2 cut(s) 753, 963
BsaJI CCNNGG 4 cut(s) 37, 274, 433, 713
BsaXI ACNNNNNCTCC 2 cut(s) 907, 937
Bsc4I CCNNNNNNNGG 2 cut(s) 365, 803
Bse1I ACTGG 3 cut(s) 292, 589, 797
Bse8I GATNNNNATC 2 cut(s) 753, 963
BseBI CCWGG 1 cut(s) 38
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 4 cut(s) 37, 274, 433, 713
BseGI GGATG 4 cut(s) 61, 232, 649, 888
BseJI GATNNNNATC 2 cut(s) 753, 963
BseLI CCNNNNNNNGG 2 cut(s) 365, 803
BseMII CTCAG 3 cut(s) 236, 387, 499
BseNI ACTGG 3 cut(s) 292, 589, 797
BseXI GCAGC 3 cut(s) 226, 229, 916
Bsh1285I CGRYCG 1 cut(s) 961
BshVI ATCGAT 1 cut(s) 957
BsiEI CGRYCG 1 cut(s) 961
BslFI GGGAC 2 cut(s) 149, 798
BslI CCNNNNNNNGG 2 cut(s) 365, 803
BsmAI GTCTC 2 cut(s) 12, 225
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 2 cut(s) 149, 798
Bsp143I GATC 5 cut(s) 25, 147, 476, 954, 958
BspACI CCGC 1 cut(s) 184
BspCNI CTCAG 3 cut(s) 235, 388, 498
BspDI ATCGAT 1 cut(s) 957
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 1 cut(s) 27
BspMAI CTGCAG 1 cut(s) 934
BspPI GGATC 3 cut(s) 20, 33, 142
BsrI ACTGG 3 cut(s) 292, 589, 797
BssECI CCNNGG 4 cut(s) 37, 274, 433, 713
BssMI GATC 5 cut(s) 25, 147, 476, 954, 958
BssT1I CCWWGG 3 cut(s) 274, 433, 713
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 256
BstDEI CTNAG 6 cut(s) 222, 324, 396, 485, 570, 762
BstENI CCTNNNNNAGG 1 cut(s) 363
BstF5I GGATG 4 cut(s) 61, 232, 649, 888
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 5 cut(s) 28, 150, 479, 957, 961
BstMAI GTCTC 2 cut(s) 12, 225
BstMBI GATC 5 cut(s) 25, 147, 476, 954, 958
BstMCI CGRYCG 1 cut(s) 961
BstMWI GCNNNNNNNGC 2 cut(s) 239, 926
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 2 cut(s) 395, 446
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 2 cut(s) 159, 930
BstV1I GCAGC 3 cut(s) 226, 229, 916
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 25
Bsu15I ATCGAT 1 cut(s) 957
BsuI GTATCC 2 cut(s) 208, 505
BsuTUI ATCGAT 1 cut(s) 957
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 4 cut(s) 61, 232, 649, 888
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 75
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 611
Csp6I GTAC 3 cut(s) 305, 592, 938
CviAII CATG 8 cut(s) 13, 188, 348, 392, 443, 452, 491, 611
CviQI GTAC 3 cut(s) 305, 592, 938
DdeI CTNAG 6 cut(s) 222, 324, 396, 485, 570, 762
DpnI GATC 5 cut(s) 27, 149, 478, 956, 960
DpnII GATC 5 cut(s) 25, 147, 476, 954, 958
DriI GACNNNNNGTC 1 cut(s) 287
Eam1105I GACNNNNNGTC 1 cut(s) 287
Eco130I CCWWGG 3 cut(s) 274, 433, 713
Eco57I CTGAAG 1 cut(s) 423
EcoNI CCTNNNNNAGG 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 3 cut(s) 274, 433, 713
ErhI CCWWGG 3 cut(s) 274, 433, 713
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 8 cut(s) 16, 191, 351, 395, 446, 455, 494, 614
FalI AAGNNNNNCTT 2 cut(s) 163, 195
FaqI GGGAC 2 cut(s) 149, 798
FatI CATG 8 cut(s) 12, 187, 347, 391, 442, 451, 490, 610
Fnu4HI GCNGC 4 cut(s) 185, 240, 243, 930
FokI GGATG 4 cut(s) 68, 239, 656, 895
Fsp4HI GCNGC 4 cut(s) 185, 240, 243, 930
FspBI CTAG 2 cut(s) 830, 854
GlaI GCGC 1 cut(s) 74
GluI GCNGC 4 cut(s) 185, 240, 243, 930
GsuI CTGGAG 1 cut(s) 255
HgaI GACGC 1 cut(s) 611
HhaI GCGC 1 cut(s) 75
Hin1II CATG 8 cut(s) 16, 191, 351, 395, 446, 455, 494, 614
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 3 cut(s) 137, 193, 749
HphI GGTGA 1 cut(s) 818
HpyAV CCTTC 3 cut(s) 359, 775, 906
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 218, 283
HpyCH4V TGCA 5 cut(s) 269, 377, 665, 920, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 926
HpyF3I CTNAG 6 cut(s) 222, 324, 396, 485, 570, 762
HpySE526I ACGT 2 cut(s) 218, 283
Hsp92II CATG 8 cut(s) 16, 191, 351, 395, 446, 455, 494, 614
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 5 cut(s) 25, 147, 476, 954, 958
LmnI GCTCC 5 cut(s) 41, 100, 236, 709, 926
Lsp1109I GCAGC 3 cut(s) 226, 229, 916
LweI GCATC 4 cut(s) 256, 386, 652, 799
MaeI CTAG 2 cut(s) 830, 854
MaeII ACGT 2 cut(s) 218, 283
MaeIII GTNAC 1 cut(s) 311
MalI GATC 5 cut(s) 27, 149, 478, 956, 960
MboI GATC 5 cut(s) 25, 147, 476, 954, 958
MboII GAAGA 3 cut(s) 416, 471, 842
MflI RGATCY 1 cut(s) 25
MluCI AATT 4 cut(s) 122, 525, 533, 677
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 846
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 2 cut(s) 537, 597
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 239, 926
NdeII GATC 5 cut(s) 25, 147, 476, 954, 958
NlaIII CATG 8 cut(s) 16, 191, 351, 395, 446, 455, 494, 614
NlaIV GGNNCC 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 2 cut(s) 395, 446
PagI TCATGA 1 cut(s) 610
PciI ACATGT 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 2 cut(s) 193, 749
PkrI GCNGC 4 cut(s) 186, 241, 244, 931
Ple19I CGATCG 1 cut(s) 961
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PscI ACATGT 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 27
PstI CTGCAG 1 cut(s) 934
PsuI RGATCY 1 cut(s) 25
PvuI CGATCG 1 cut(s) 961
RsaI GTAC 3 cut(s) 306, 593, 939
RsaNI GTAC 3 cut(s) 305, 592, 938
SaqAI TTAA 2 cut(s) 537, 597
SatI GCNGC 4 cut(s) 185, 240, 243, 930
Sau3AI GATC 5 cut(s) 25, 147, 476, 954, 958
ScaI AGTACT 1 cut(s) 939
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 38
SfaNI GCATC 4 cut(s) 256, 386, 652, 799
SfcI CTRYAG 2 cut(s) 159, 930
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
Sse9I AATT 4 cut(s) 122, 525, 533, 677
SsiI CCGC 1 cut(s) 184
SspMI CTAG 2 cut(s) 830, 854
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 3 cut(s) 274, 433, 713
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 2 cut(s) 221, 286
TaqI TCGA 3 cut(s) 475, 957, 961
TasI AATT 4 cut(s) 122, 525, 533, 677
TatI WGTACW 3 cut(s) 304, 591, 937
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 2 cut(s) 193, 749
Tru1I TTAA 2 cut(s) 537, 597
Tru9I TTAA 2 cut(s) 537, 597
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 3 cut(s) 239, 242, 929
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 185, 243
TspGWI ACGGA 1 cut(s) 934
XagI CCTNNNNNAGG 1 cut(s) 363
XapI RAATTY 3 cut(s) 122, 525, 677
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 2 cut(s) 395, 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 2 cut(s) 830, 854
ZrmI AGTACT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.