MD16G1253700.v1.1

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001569
Physical Location & Seq
Forward (+)
28852718 .. 28865589
12872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1253700.v1.1.491

Sequence Viewer

Length: 2883 bp
ATGGCTGTTTCCATGAGAGATTTAGATCCAGCTTTCCAGGGAGCTGGACAAAAGGCTGGACTTGAAATATGGCGCATTGAAAACTTTCGTCCTGCTCCAGTCCCAAAATCCTCTTATGGCAAGTTTTTTATGGGGGACTCTTATGTGATCCTAAAAACAACAGCCTCAAAAAGTGGTGCCTTGCGGCATGAAATCCATTATTGGCTTGGCAAGGATACTTCCCAGGATGAAGCTGGGGCTGCAGCCATCAAGACAGTTGAATTAGATGCAACCATTGGGGGACGTGCTGTCCAGCATCGTGAAGTACAGGGTCACGAAACTGCCAAGTTCCTATCTTATTTCAAACCATGTATCATACCTCAAGAGGGTGGAGTTGCATCTGGTTTCAAACATGCTGTGGCTGAAGAACACAAGACACGATTGTTTGTGTGCAAAGGAAAACATGTTGTCCATGTCAAAGAGGTTGCTTTTGCTCGATCTTCACTCAGCCATGATGACATTTTTATTTTGGATACGAAGTGTAAAATTTTTCAATTTAATGGTTCGAACTCATCTATTCAAGAAAGAGCTAAGGCGTTAGAAGTTGTCCAGTACATTAAAGACACATATCATGATGGGAAATGTGAGATAGCTTCCATTGAGGATGGAAAGTTGATGGCTGATGCTGAAAGTGGAGAATTTTGGGGTTTATTTGGTGGTTTTGCTCCACTTCCAAAGAAAACAGCCACCGATGAGGACAAAAGTTTTGATTCTTATCCTACTAAGCTTCTTTGTGTTGTGAAGGGACAGGCAGAACCAGTTGAGGGTGATGCTTTAACGAGGGATTTGCTAGACACAAATAAGTGCTATCTTCTAGATTGTGGGTTGGAAGTGTTTGTTTGGATGGGGAGAAATACATCTCTTGACGAAAGAAGGAGTGCAAGTGGAGCTGCAGAAGAGTTAGTTCATGGTCCTGATCGATCGAAATCCCACATAATTCGTGTAATTGAGGGCTTTGAGACAGTAATGTTTAGGTCTAAATTCAATTCATGGCCCCAAACAGCTGATGTAGCTGTGTCAGAAGATGGTAGGGGCAAGGTTGCAGCACTCTTAAAGCGTCAAGGGGTTAATGTGAAGGGTCTGTTGAAAGCTGATCCTGTAAAAGAAGAACCTCAACCATACATTGATTGCACAGGAAACTTGCAGGTTTGGCGTGTGAATGGCCAGGAAAAGATTAGCCTTCAATCTTCTGATCAATCAAAATTTTACAGTGGAGATTGCTATATTTTCCATTATTCATATCCAGGAGAGGATAAAGAGGAACATCTTATAGGAACATGGTTTGGAAAGCAAAGCGTTGAGGAAGAAAGAGCATCAGCTATTTCACTAGCAAGCAAGATGGTTGAGTCAATGAAGTTTCTTGCTGCCCAGGCTCGTATCTATGAAGGAAATGAACCAATTCAGTTCTATTCAATCTTCCAAAGCATAATTGTTTTGAAGGGTGGTCTCAGTGATGGATACAAAAGTTATGTTGTGGAGAGAGAAGTTGCAGATGAGACATATCAAGAAGATGGTGTTGCATTATTTCGTGTTCAGGGATCTGGACCTGATAATATGCAAGCAATACAAGTTGATGCAGTTGCATCATCTTTGAATTCCTCATACTGTTATATATTACATAGTGGCTCAACTGTCTTTACCTGGTCTGGAAGCCTCACAAACTCAGATGATCAGGAACTCGTTGAGAGGCAGCTCGATTTAATAAAGCCGGATCTTCAGTCGAAAACGCAAAAGGAAAATTCCGAGTCTGAACAGTTTTGGGAGTTATTAGGAGGAAAGACCGAGTATCCCAGCCAAAAGATTGTACGATCTTCCGAAAGTGACCCTCGCCTATTTTCTTGCACCTTCTCAAATGGAAATTTGAAGGTAGTAGAGATATACAACTTCACCCAGGACGATTTGATGACTGAAGATATATTCATCTTGGATTGTCACTCAGACATCTTCGTTTGGGTCGGCCAACAGGTCAAATCCAAGGATAGGATGCAGGCTTTAACTATTGGGGAGAAATTTCTTGAGCATGATTTTTTAATGGAAAAATTATCTCGTGAAGCTTCAATATATATCGTCATGGAAGGAAGTGAGCCACCTTTCTTCACACGCTTCTTCATTTGGGACTCTGCAAAATCTGCTATGCATGGGAACTCTTTCCAAAGGAAGCTTACTATACTAAAAAATGGGGGTACTCCAACTCTAAATAAACCAAAACGGAGAGCACCAGTATCTTATGGTGGAAGGTCTAGTGTACCAGATAAATCACAGCGTTCCAGAAGCATGTCTTTCAGCCCCGATCGAGTTCGTGTGAGGGGTAGATCTCCAGCTTTCAATGCACTGGCTGCTACTTTTGAGAATGCTAATGTCAGAAACCTTTCAACTCCACCTCCAATGGTCAGAAAGCTGTACCCCAAATCTGTGACTCCAGATTCATCAAAATTGGCTTCAAAATCATCAGCTATAGCAGCACTCACTGCTAGCTTCGAGAAAACAGGACCAGCAAGAGAATCTAATATACCAAGATCGCCGAAAGTGAGCCAAGGGCCCCCCAAACCAAAACAACAGGAAACGAATAACAAGGAGAATTCTGTGAGCGGTAAACTAGAATCCCTCACCATACACGAAGATGTGAAGGAGGGTGAAGCAGAAGATGAGAACCTCCCAGTTTACCCATATGAACGCCTTAAAACAACATCACAAGATCCTGTTACAGATATTGATGTGACGAAGCGAGAGATTTACTTGTCAGCAGAGGAGTTCAGGGAGCATTTCGGGATGGCAAAGGATGCTTTCCATAAGTTTCCCAAATGGAAACAGAACAAGCTTAAAATGGCCCTTCAATTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

961

Amino Acids

107.35

Weight (kDa)

6.23

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 29 - 111 6.7e-14 Gelsolin repeat
Gelsolin PF00626 149 - 216 1.7e-09 Gelsolin repeat
Gelsolin PF00626 267 - 334 1.6e-08 Gelsolin repeat
Gelsolin PF00626 635 - 712 4e-09 Gelsolin repeat
VHP PF02209 925 - 960 7.8e-13 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1174
AccB1I GGYRCC 1 cut(s) 176
AccBSI CCGCTC 1 cut(s) 2628
AciI CCGC 2 cut(s) 184, 2628
AclWI GGATC 6 cut(s) 20, 142, 1127, 1585, 1758, 2729
AcoI YGGCCR 2 cut(s) 1201, 1996
AcsI RAATTY 9 cut(s) 525, 677, 1019, 1241, 1633, 1777, 1897, 2048, 2617
AcuI CTGAAG 3 cut(s) 423, 1739, 1968
AfaI GTAC 6 cut(s) 306, 593, 1845, 2224, 2286, 2441
AfiI CCNNNNNNNGG 3 cut(s) 365, 803, 2019
AflIII ACRYGT 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 287
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 7 cut(s) 36, 222, 1203, 1282, 1407, 1679, 1929
Alw21I GWGCWC 1 cut(s) 2257
Alw26I GTCTC 3 cut(s) 992, 1490, 1529
AlwI GGATC 6 cut(s) 20, 142, 1127, 1585, 1758, 2729
AoxI GGCC 5 cut(s) 1031, 1201, 1996, 2576, 2865
ApaI GGGCCC 1 cut(s) 2580
ApeKI GCWGC 8 cut(s) 239, 242, 929, 1082, 1403, 1729, 2375, 2498
ApoI RAATTY 9 cut(s) 525, 677, 1019, 1241, 1633, 1777, 1897, 2048, 2617
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
Asp700I GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 7 cut(s) 950, 1032, 1583, 2528, 2576, 2577, 2866
AsuHPI GGTGA 4 cut(s) 818, 1918, 2638, 2684
AsuII TTCGAA 1 cut(s) 545
AsuNHI GCTAGC 1 cut(s) 2510
AvaII GGWCC 3 cut(s) 950, 1583, 2528
BaeGI GKGCMC 1 cut(s) 2580
BaeI ACNNNNGTAYC 2 cut(s) 1489, 1522
BalI TGGCCA 1 cut(s) 1203
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 2580
BauI CACGAG 1 cut(s) 2085
Bbv12I GWGCWC 1 cut(s) 2257
BbvI GCAGC 8 cut(s) 226, 254, 916, 1094, 1390, 1741, 2362, 2510
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BciT130I CCWGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
BciVI GTATCC 4 cut(s) 208, 505, 1490, 1836
BclI TGATCA 2 cut(s) 1231, 1708
BcoDI GTCTC 3 cut(s) 992, 1490, 1529
BfaI CTAG 6 cut(s) 830, 854, 1367, 2280, 2511, 2636
BfmI CTRYAG 3 cut(s) 240, 930, 2493
BfuAI ACCTGC 1 cut(s) 1174
BfuI GTATCC 4 cut(s) 208, 505, 1490, 1836
BglII AGATCT 1 cut(s) 2351
BisI GCNGC 9 cut(s) 185, 240, 243, 930, 1083, 1404, 1730, 2376, 2499
BlsI GCNGC 9 cut(s) 186, 241, 244, 931, 1084, 1405, 1731, 2377, 2500
Bme1390I CCNGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
Bme18I GGWCC 3 cut(s) 950, 1583, 2528
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 7 cut(s) 950, 1032, 1583, 2528, 2576, 2577, 2866
BmiI GGNNCC 4 cut(s) 178, 1034, 2578, 2579
BmrFI CCNGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
BmrI ACTGGG 1 cut(s) 2690
BmtI GCTAGC 1 cut(s) 2514
BmuI ACTGGG 1 cut(s) 2690
BpmI CTGGAG 3 cut(s) 81, 2340, 2442
Bpu10I CCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 545
BpuEI CTTGAG 2 cut(s) 345, 2075
Bsa29I ATCGAT 1 cut(s) 958
BsaBI GATNNNNATC 3 cut(s) 24, 753, 964
BsaI GGTCTC 1 cut(s) 1490
BsaJI CCNNGG 6 cut(s) 37, 222, 1407, 1929, 2013, 2572
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 2404, 2434
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 803, 2019
Bse1I ACTGG 6 cut(s) 98, 589, 797, 2258, 2376, 2696
Bse8I GATNNNNATC 3 cut(s) 24, 753, 964
BseBI CCWGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
BseCI ATCGAT 1 cut(s) 958
BseDI CCNNGG 6 cut(s) 37, 222, 1407, 1929, 2013, 2572
BseGI GGATG 6 cut(s) 232, 649, 888, 2028, 2814, 2824
BseJI GATNNNNATC 3 cut(s) 24, 753, 964
BseLI CCNNNNNNNGG 3 cut(s) 365, 803, 2019
BseMII CTCAG 4 cut(s) 499, 1501, 1716, 1989
BseNI ACTGG 6 cut(s) 98, 589, 797, 2258, 2376, 2696
BseRI GAGGAG 1 cut(s) 2801
BseSI GKGCMC 1 cut(s) 2580
BseXI GCAGC 8 cut(s) 226, 254, 916, 1094, 1390, 1741, 2362, 2510
BseYI CCCAGC 2 cut(s) 233, 1829
Bsh1285I CGRYCG 2 cut(s) 962, 2332
BshFI GGCC 5 cut(s) 1033, 1203, 1998, 2578, 2867
BshNI GGYRCC 1 cut(s) 176
BshVI ATCGAT 1 cut(s) 958
BsiEI CGRYCG 2 cut(s) 962, 2332
BsiHKAI GWGCWC 1 cut(s) 2257
BsiSI CCGG 1 cut(s) 1748
BslFI GGGAC 5 cut(s) 86, 149, 294, 798, 2168
BslI CCNNNNNNNGG 3 cut(s) 365, 803, 2019
BsmAI GTCTC 3 cut(s) 992, 1490, 1529
BsmFI GGGAC 5 cut(s) 86, 149, 294, 798, 2168
BsmI GAATGC 1 cut(s) 2395
BsnI GGCC 5 cut(s) 1033, 1203, 1998, 2578, 2867
Bso31I GGTCTC 1 cut(s) 1490
Bsp119I TTCGAA 1 cut(s) 545
Bsp120I GGGCCC 1 cut(s) 2576
Bsp1286I GDGCHC 2 cut(s) 2257, 2580
BspACI CCGC 2 cut(s) 184, 2628
BspANI GGCC 5 cut(s) 1033, 1203, 1998, 2578, 2867
BspCNI CTCAG 4 cut(s) 498, 1500, 1715, 1988
BspDI ATCGAT 1 cut(s) 958
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 4 cut(s) 178, 1034, 2578, 2579
BspMAI CTGCAG 2 cut(s) 244, 934
BspMI ACCTGC 1 cut(s) 1174
BspOI GCTAGC 1 cut(s) 2514
BspPI GGATC 6 cut(s) 20, 142, 1127, 1585, 1758, 2729
BspT104I TTCGAA 1 cut(s) 545
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 1490
BsrBI CCGCTC 1 cut(s) 2628
BsrI ACTGG 6 cut(s) 98, 589, 797, 2258, 2376, 2696
BssECI CCNNGG 6 cut(s) 37, 222, 1407, 1929, 2013, 2572
BssSI CACGAG 1 cut(s) 2085
BssT1I CCWWGG 2 cut(s) 2013, 2572
Bst2BI CACGAG 1 cut(s) 2085
Bst2UI CCWGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
Bst4CI ACNGT 6 cut(s) 256, 1003, 1250, 1646, 1672, 1794
Bst6I CTCTTC 1 cut(s) 930
BstAPI GCANNNNNTGC 4 cut(s) 2168, 2375, 2507, 2819
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 4 cut(s) 1372, 1599, 2028, 2512
BstDEI CTNAG 6 cut(s) 485, 570, 762, 1487, 1702, 1975
BstF5I GGATG 6 cut(s) 232, 649, 888, 2028, 2814, 2824
BstHHI GCGC 1 cut(s) 75
BstMAI GTCTC 3 cut(s) 992, 1490, 1529
BstMCI CGRYCG 2 cut(s) 962, 2332
BstNI CCWGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
BstNSI RCATGY 3 cut(s) 395, 446, 2317
BstSCI CCNGG 7 cut(s) 36, 222, 1203, 1282, 1407, 1679, 1929
BstSFI CTRYAG 3 cut(s) 240, 930, 2493
BstSLI GKGCMC 1 cut(s) 2580
BstV1I GCAGC 8 cut(s) 226, 254, 916, 1094, 1390, 1741, 2362, 2510
BstX2I RGATCY 5 cut(s) 25, 1577, 1750, 2351, 2734
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 5 cut(s) 25, 1577, 1750, 2351, 2734
Bsu15I ATCGAT 1 cut(s) 958
BsuI GTATCC 4 cut(s) 208, 505, 1490, 1836
BsuRI GGCC 5 cut(s) 1033, 1203, 1998, 2578, 2867
BsuTUI ATCGAT 1 cut(s) 958
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 6 cut(s) 232, 649, 888, 2028, 2814, 2824
BtsI GCAGTG 1 cut(s) 2505
BtsIMutI CAGTG 4 cut(s) 1255, 1495, 2369, 2505
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 4 cut(s) 1372, 1599, 2028, 2512
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 7 cut(s) 950, 1032, 1583, 2528, 2576, 2577, 2866
ClaI ATCGAT 1 cut(s) 958
CseI GACGC 1 cut(s) 1085
CsiI ACCWGGT 1 cut(s) 1679
Csp6I GTAC 6 cut(s) 305, 592, 1844, 2223, 2285, 2440
CviQI GTAC 6 cut(s) 305, 592, 1844, 2223, 2285, 2440
DdeI CTNAG 6 cut(s) 485, 570, 762, 1487, 1702, 1975
DriI GACNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 2 cut(s) 1201, 1996
Eam1104I CTCTTC 1 cut(s) 930
Eam1105I GACNNNNNGTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 930
Eco130I CCWWGG 2 cut(s) 2013, 2572
Eco24I GRGCYC 1 cut(s) 2580
Eco31I GGTCTC 1 cut(s) 1490
Eco47I GGWCC 3 cut(s) 950, 1583, 2528
Eco57I CTGAAG 3 cut(s) 423, 1739, 1968
EcoO109I RGGNCCY 2 cut(s) 2576, 2577
EcoRI GAATTC 2 cut(s) 1633, 2617
EcoRII CCWGG 7 cut(s) 36, 222, 1203, 1282, 1407, 1679, 1929
EcoT14I CCWWGG 2 cut(s) 2013, 2572
EcoT22I ATGCAT 1 cut(s) 2178
EcoT38I GRGCYC 1 cut(s) 2580
ErhI CCWWGG 2 cut(s) 2013, 2572
FalI AAGNNNNNCTT 2 cut(s) 2302, 2334
FaqI GGGAC 5 cut(s) 86, 149, 294, 798, 2168
FauNDI CATATG 1 cut(s) 2707
FbaI TGATCA 2 cut(s) 1231, 1708
Fnu4HI GCNGC 9 cut(s) 185, 240, 243, 930, 1083, 1404, 1730, 2376, 2499
FokI GGATG 6 cut(s) 239, 656, 895, 2035, 2821, 2831
FriOI GRGCYC 1 cut(s) 2580
Fsp4HI GCNGC 9 cut(s) 185, 240, 243, 930, 1083, 1404, 1730, 2376, 2499
FspBI CTAG 6 cut(s) 830, 854, 1367, 2280, 2511, 2636
GlaI GCGC 1 cut(s) 74
GluI GCNGC 9 cut(s) 185, 240, 243, 930, 1083, 1404, 1730, 2376, 2499
GsaI CCCAGC 2 cut(s) 237, 1833
GsuI CTGGAG 3 cut(s) 81, 2340, 2442
HaeIII GGCC 5 cut(s) 1033, 1203, 1998, 2578, 2867
HapII CCGG 1 cut(s) 1748
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 1 cut(s) 75
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 4 cut(s) 764, 2091, 2198, 2855
HinfI GANTC 9 cut(s) 137, 749, 1385, 1784, 2156, 2455, 2462, 2540, 2639
HpaII CCGG 1 cut(s) 1748
HphI GGTGA 4 cut(s) 818, 1918, 2638, 2684
Hpy166II GTNNAC 3 cut(s) 2285, 2633, 2701
Hpy188I TCNGA 9 cut(s) 1060, 1231, 1705, 1783, 1789, 1855, 1978, 2402, 2432
Hpy8I GTNNAC 3 cut(s) 2285, 2633, 2701
HpyCH4III ACNGT 6 cut(s) 256, 1003, 1250, 1646, 1672, 1794
HpyCH4IV ACGT 1 cut(s) 283
HpyF3I CTNAG 6 cut(s) 485, 570, 762, 1487, 1702, 1975
HpySE526I ACGT 1 cut(s) 283
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 2 cut(s) 1231, 1708
LmnI GCTCC 5 cut(s) 41, 100, 709, 926, 2797
Lsp1109I GCAGC 8 cut(s) 226, 254, 916, 1094, 1390, 1741, 2362, 2510
MabI ACCWGGT 1 cut(s) 1679
MaeI CTAG 6 cut(s) 830, 854, 1367, 2280, 2511, 2636
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 6 cut(s) 311, 1859, 1970, 2452, 2740, 2755
MbiI CCGCTC 1 cut(s) 2628
MfeI CAATTG 1 cut(s) 2873
MflI RGATCY 5 cut(s) 25, 1577, 1750, 2351, 2734
MhlI GDGCHC 2 cut(s) 2257, 2580
MlsI TGGCCA 1 cut(s) 1203
MluNI TGGCCA 1 cut(s) 1203
MlyI GAGTC 5 cut(s) 131, 1394, 1793, 2150, 2449
MmeI TCCRAC 2 cut(s) 846, 2252
Mox20I TGGCCA 1 cut(s) 1203
Mph1103I ATGCAT 1 cut(s) 2178
MroXI GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
MscI TGGCCA 1 cut(s) 1203
MslI CAYNNNNRTG 1 cut(s) 2658
Msp20I TGGCCA 1 cut(s) 1203
MspA1I CMGCKG 1 cut(s) 1043
MspI CCGG 1 cut(s) 1748
MspR9I CCNGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
MunI CAATTG 1 cut(s) 2873
Mva1269I GAATGC 1 cut(s) 2395
MvaI CCWGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
NdeI CATATG 1 cut(s) 2707
NheI GCTAGC 1 cut(s) 2510
NlaIV GGNNCC 4 cut(s) 178, 1034, 2578, 2579
NmuCI GTSAC 5 cut(s) 311, 1859, 1970, 2452, 2755
NsiI ATGCAT 1 cut(s) 2178
NspI RCATGY 3 cut(s) 395, 446, 2317
NspV TTCGAA 1 cut(s) 545
PagI TCATGA 1 cut(s) 610
PciI ACATGT 1 cut(s) 442
PctI GAATGC 1 cut(s) 2395
PdmI GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
PfeI GAWTC 4 cut(s) 749, 2462, 2540, 2639
PfoI TCCNGGA 1 cut(s) 1282
PkrI GCNGC 9 cut(s) 186, 241, 244, 931, 1084, 1405, 1731, 2377, 2500
Ple19I CGATCG 2 cut(s) 962, 2332
PleI GAGTC 5 cut(s) 131, 1393, 1792, 2150, 2449
PpsI GAGTC 5 cut(s) 131, 1393, 1792, 2150, 2449
PscI ACATGT 1 cut(s) 442
Psp6I CCWGG 7 cut(s) 36, 222, 1203, 1282, 1407, 1679, 1929
PspFI CCCAGC 2 cut(s) 233, 1829
PspGI CCWGG 7 cut(s) 36, 222, 1203, 1282, 1407, 1679, 1929
PspN4I GGNNCC 4 cut(s) 178, 1034, 2578, 2579
PspOMI GGGCCC 1 cut(s) 2576
PspPI GGNCC 7 cut(s) 950, 1032, 1583, 2528, 2576, 2577, 2866
PstI CTGCAG 2 cut(s) 244, 934
PsuI RGATCY 5 cut(s) 25, 1577, 1750, 2351, 2734
PvuI CGATCG 2 cut(s) 962, 2332
PvuII CAGCTG 1 cut(s) 1043
RsaI GTAC 6 cut(s) 306, 593, 1845, 2224, 2286, 2441
RsaNI GTAC 6 cut(s) 305, 592, 1844, 2223, 2285, 2440
RseI CAYNNNNRTG 1 cut(s) 2658
SatI GCNGC 9 cut(s) 185, 240, 243, 930, 1083, 1404, 1730, 2376, 2499
Sau96I GGNCC 7 cut(s) 950, 1032, 1583, 2528, 2576, 2577, 2866
SchI GAGTC 5 cut(s) 131, 1394, 1793, 2150, 2449
ScrFI CCNGG 7 cut(s) 38, 224, 1205, 1284, 1409, 1681, 1931
SduI GDGCHC 2 cut(s) 2257, 2580
SexAI ACCWGGT 1 cut(s) 1679
SfcI CTRYAG 3 cut(s) 240, 930, 2493
SfuI TTCGAA 1 cut(s) 545
SinI GGWCC 3 cut(s) 950, 1583, 2528
SmiMI CAYNNNNRTG 1 cut(s) 2658
SmlI CTYRAG 2 cut(s) 360, 2054
SmoI CTYRAG 2 cut(s) 360, 2054
SsiI CCGC 2 cut(s) 184, 2628
SspMI CTAG 6 cut(s) 830, 854, 1367, 2280, 2511, 2636
StyD4I CCNGG 7 cut(s) 36, 222, 1203, 1282, 1407, 1679, 1929
StyI CCWWGG 2 cut(s) 2013, 2572
TaaI ACNGT 6 cut(s) 256, 1003, 1250, 1646, 1672, 1794
TaiI ACGT 1 cut(s) 286
TaqI TCGA 8 cut(s) 475, 545, 958, 962, 1734, 1760, 2332, 2517
TaqII GACCGA 1 cut(s) 1835
TatI WGTACW 2 cut(s) 304, 591
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 4 cut(s) 749, 2462, 2540, 2639
TscAI CASTG 4 cut(s) 1255, 1495, 2376, 2512
TseFI GTSAC 5 cut(s) 311, 1859, 1970, 2452, 2755
TseI GCWGC 8 cut(s) 239, 242, 929, 1082, 1403, 1729, 2375, 2498
Tsp45I GTSAC 5 cut(s) 311, 1859, 1970, 2452, 2755
TspGWI ACGGA 1 cut(s) 2263
TspRI CASTG 4 cut(s) 1255, 1495, 2376, 2512
VpaK11BI GGWCC 3 cut(s) 950, 1583, 2528
XapI RAATTY 9 cut(s) 525, 677, 1019, 1241, 1633, 1777, 1897, 2048, 2617
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 3 cut(s) 395, 446, 2317
XcmI CCANNNNNNNNNTGG 2 cut(s) 203, 230
XmnI GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
XspI CTAG 6 cut(s) 830, 854, 1367, 2280, 2511, 2636
Zsp2I ATGCAT 1 cut(s) 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.