pycom13g21730

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001569
Physical Location & Seq
Forward (+)
18657834 .. 18661748
3915 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21730.1

Sequence Viewer

Length: 1179 bp
ATGATCGATGAACTTGTTGAGAGGCAGCTCGATTTAATAAAGCCGGATCTCCAGTCGAAAATGCAAAAGGAAAATTCCGAGTCTGAACAGTTTTGGGAGTTGTTAGGAGGAAAGACCGAGTATCCCAGCCAAAAGATTGTAAGATCTGGTGAAAGCGACCCCCGCCTATTTTCTTGCACCTTCTCAAATGGAAATTTGAAGGTAGTAGAGATATACAACTTCACCCAGGATGATTTGATGACTGAAGATATATTCATCTTGGATTGTCATGTAGACATCTTTGTTTGGGTTGGCCAACAGGTCAACTTCAAGGATAGGATGCAGGCTTTAACTATTGGGGAGAAATTTCTTGAGCATGATTTTTTCATGGAACAATTATCTCGTGAAGCTTCAATATATATCATCATGGAAGGAAGTGAGCCACCTTTCTTCACACGGTTCTTCACTTGGGACTCTGCAAAATCTTCTATGCACGGGAACTCTTTCCAAAGGAAACTTACTATACTGAAAAATGGGGGTAGTCCAACTGTAAATAAACCAAAACGGAGAGCACCTGTATCCTATGGTGGAAGGTCTAGTGCACCAGAAAAATCACAGCGTTCCAGAAGCATGTCTTTCAGCCCTGATCGAGTCCGTGTGAGGGGTAGATCTCCAGCTTTCAATGCACTGGCTGCTACTTTTGAGAATGCTAATGCCAGAAACCTTTCAACTCCACCTCCAATGGTCACAAAACTGTACCCCAAATCTGTGACTCCAGATTCATCAAAATTGGCTTCAAAATCATCTGCTATAGCAGCACTCACTGCTAGTTTTGATAAAACAGGACCAGCAAGAGAATCTAATATACCAAGATCGCCGAAAATGAGCCCAGGGCCCCCCAAGCCAAAACAACAGGAGACAAATAACAAGGAGAATTCTATGACCAGTAAACTAGAATCCCTCACCATACAGGAAGATTCGAAGGAGGGTGAAGCAGAAGATGAGAACCTCCCAGTTCACCCATATGGACGCCTTAAAACAACATCAGAAGATCCTGTTACGGATATTGATGTGACGAAGCGAGAGATTTACTTGTCAGCAGAGGAGTTCAGGGAGCATTTCGGTATGGCAAAGGATGCTTTCCATAAGTTGCCCAAGTGGAAACAGAACAAGCTTAAAATGGCCCTTCTATTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

393

Amino Acids

44.44

Weight (kDa)

8.29

Isoelectric Point (pI)

57.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 67 - 144 7e-09 Gelsolin repeat
VHP PF02209 357 - 392 3e-14 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 273
AciI CCGC 1 cut(s) 163
AclWI GGATC 2 cut(s) 54, 1025
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 4 cut(s) 73, 193, 344, 913
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 1009
AfaI GTAC 1 cut(s) 737
AfiI CCNNNNNNNGG 1 cut(s) 640
AgsI TTSAA 6 cut(s) 199, 310, 393, 661, 708, 777
AjnI CCWGG 2 cut(s) 225, 868
AluBI AGCT 4 cut(s) 28, 389, 656, 1153
AluI AGCT 4 cut(s) 28, 389, 656, 1153
Alw21I GWGCWC 2 cut(s) 553, 583
Alw26I GTCTC 1 cut(s) 890
Alw44I GTGCAC 1 cut(s) 579
AlwI GGATC 2 cut(s) 54, 1025
AoxI GGCC 3 cut(s) 292, 872, 1161
ApaI GGGCCC 1 cut(s) 876
ApaLI GTGCAC 1 cut(s) 579
ApeKI GCWGC 3 cut(s) 25, 671, 794
ApoI RAATTY 4 cut(s) 73, 193, 344, 913
Asp700I GAANNNNTTC 2 cut(s) 482, 703
AspS9I GGNCC 4 cut(s) 824, 872, 873, 1162
AsuHPI GGTGA 5 cut(s) 161, 214, 934, 980, 989
AsuII TTCGAA 1 cut(s) 959
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 2 cut(s) 583, 876
BalI TGGCCA 1 cut(s) 294
BanII GRGCYC 2 cut(s) 869, 876
BauI CACGAG 1 cut(s) 381
Bbv12I GWGCWC 2 cut(s) 553, 583
BbvI GCAGC 3 cut(s) 37, 658, 806
BciT130I CCWGG 2 cut(s) 227, 870
BciVI GTATCC 2 cut(s) 132, 568
BcoDI GTCTC 1 cut(s) 890
BfaI CTAG 3 cut(s) 576, 807, 932
BfmI CTRYAG 1 cut(s) 789
BfuI GTATCC 2 cut(s) 132, 568
BglII AGATCT 2 cut(s) 143, 647
BisI GCNGC 3 cut(s) 26, 672, 795
BlsI GCNGC 3 cut(s) 27, 673, 796
Bme1390I CCNGG 2 cut(s) 227, 870
Bme18I GGWCC 1 cut(s) 824
BmgT120I GGNCC 4 cut(s) 824, 872, 873, 1162
BmiI GGNNCC 2 cut(s) 874, 875
BmrFI CCNGG 2 cut(s) 227, 870
BmrI ACTGGG 1 cut(s) 986
BmsI GCATC 2 cut(s) 309, 1105
BmuI ACTGGG 1 cut(s) 986
BpmI CTGGAG 3 cut(s) 35, 636, 738
Bpu14I TTCGAA 1 cut(s) 959
BpuEI CTTGAG 1 cut(s) 371
Bsa29I ATCGAT 1 cut(s) 6
BsaHI GRCGYC 1 cut(s) 1009
BsaJI CCNNGG 3 cut(s) 225, 868, 869
BsaXI ACNNNNNCTCC 2 cut(s) 700, 730
Bsc4I CCNNNNNNNGG 1 cut(s) 640
Bse1I ACTGG 4 cut(s) 52, 672, 924, 992
BseBI CCWGG 2 cut(s) 227, 870
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 225, 868, 869
BseGI GGATG 3 cut(s) 235, 324, 1120
BseLI CCNNNNNNNGG 1 cut(s) 640
BseNI ACTGG 4 cut(s) 52, 672, 924, 992
BseRI GAGGAG 1 cut(s) 1097
BseSI GKGCMC 2 cut(s) 583, 876
BseXI GCAGC 3 cut(s) 37, 658, 806
BseYI CCCAGC 1 cut(s) 125
BshFI GGCC 3 cut(s) 294, 874, 1163
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 2 cut(s) 553, 583
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 1 cut(s) 640
BsmAI GTCTC 1 cut(s) 890
BsmFI GGGAC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 691
BsnI GGCC 3 cut(s) 294, 874, 1163
Bsp119I TTCGAA 1 cut(s) 959
Bsp120I GGGCCC 1 cut(s) 872
Bsp1286I GDGCHC 4 cut(s) 553, 583, 869, 876
Bsp143I GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
BspACI CCGC 1 cut(s) 163
BspANI GGCC 3 cut(s) 294, 874, 1163
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 2 cut(s) 874, 875
BspPI GGATC 2 cut(s) 54, 1025
BspT104I TTCGAA 1 cut(s) 959
BsrI ACTGG 4 cut(s) 52, 672, 924, 992
BssECI CCNNGG 3 cut(s) 225, 868, 869
BssMI GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
BssNI GRCGYC 1 cut(s) 1009
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 2 cut(s) 227, 870
Bst4CI ACNGT 4 cut(s) 90, 438, 529, 735
BstACI GRCGYC 1 cut(s) 1009
BstAPI GCANNNNNTGC 3 cut(s) 671, 803, 1115
BstBI TTCGAA 1 cut(s) 959
BstC8I GCNNGC 1 cut(s) 324
BstF5I GGATG 3 cut(s) 235, 324, 1120
BstKTI GATC 7 cut(s) 6, 49, 146, 628, 650, 854, 1033
BstMAI GTCTC 1 cut(s) 890
BstMBI GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
BstMWI GCNNNNNNNGC 7 cut(s) 162, 662, 671, 794, 803, 880, 1115
BstNI CCWGG 2 cut(s) 227, 870
BstNSI RCATGY 1 cut(s) 613
BstSCI CCNGG 2 cut(s) 225, 868
BstSFI CTRYAG 1 cut(s) 789
BstSLI GKGCMC 2 cut(s) 583, 876
BstV1I GCAGC 3 cut(s) 37, 658, 806
BstX2I RGATCY 4 cut(s) 46, 143, 647, 1030
BstYI RGATCY 4 cut(s) 46, 143, 647, 1030
Bsu15I ATCGAT 1 cut(s) 6
BsuI GTATCC 2 cut(s) 132, 568
BsuRI GGCC 3 cut(s) 294, 874, 1163
BsuTUI ATCGAT 1 cut(s) 6
BtsCI GGATG 3 cut(s) 235, 324, 1120
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 2 cut(s) 665, 801
Cac8I GCNNGC 1 cut(s) 324
Cfr13I GGNCC 4 cut(s) 824, 872, 873, 1162
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 1 cut(s) 1017
Csp6I GTAC 1 cut(s) 736
CviAII CATG 5 cut(s) 269, 356, 367, 406, 610
CviQI GTAC 1 cut(s) 736
DpnI GATC 7 cut(s) 5, 48, 145, 627, 649, 853, 1032
DpnII GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
EaeI YGGCCR 1 cut(s) 292
Eco24I GRGCYC 2 cut(s) 869, 876
Eco47I GGWCC 1 cut(s) 824
Eco57I CTGAAG 1 cut(s) 264
EcoO109I RGGNCCY 2 cut(s) 872, 873
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 2 cut(s) 225, 868
EcoT38I GRGCYC 2 cut(s) 869, 876
FaeI CATG 5 cut(s) 272, 359, 370, 409, 613
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FaqI GGGAC 1 cut(s) 464
FatI CATG 5 cut(s) 268, 355, 366, 405, 609
FauI CCCGC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 1003
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 26, 672, 795
FokI GGATG 3 cut(s) 242, 331, 1127
FriOI GRGCYC 2 cut(s) 869, 876
Fsp4HI GCNGC 3 cut(s) 26, 672, 795
FspBI CTAG 3 cut(s) 576, 807, 932
GluI GCNGC 3 cut(s) 26, 672, 795
GsaI CCCAGC 1 cut(s) 129
GsuI CTGGAG 3 cut(s) 35, 636, 738
HaeIII GGCC 3 cut(s) 294, 874, 1163
HapII CCGG 1 cut(s) 44
HgaI GACGC 1 cut(s) 1017
Hin1I GRCGYC 1 cut(s) 1009
Hin1II CATG 5 cut(s) 272, 359, 370, 409, 613
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 2 cut(s) 387, 1151
HinfI GANTC 8 cut(s) 80, 452, 630, 751, 758, 836, 935, 956
HpaII CCGG 1 cut(s) 44
HphI GGTGA 5 cut(s) 161, 214, 934, 980, 989
Hpy166II GTNNAC 5 cut(s) 274, 304, 581, 929, 997
Hpy188I TCNGA 4 cut(s) 79, 85, 1027, 1178
Hpy188III TCNNGA 4 cut(s) 350, 383, 603, 755
Hpy8I GTNNAC 5 cut(s) 274, 304, 581, 929, 997
HpyAV CCTTC 6 cut(s) 190, 193, 404, 564, 955, 1175
HpyCH4III ACNGT 4 cut(s) 90, 438, 529, 735
HpyCH4V TGCA 7 cut(s) 64, 177, 322, 458, 472, 581, 665
HpyF10VI GCNNNNNNNGC 7 cut(s) 162, 662, 671, 794, 803, 880, 1115
Hsp92I GRCGYC 1 cut(s) 1009
Hsp92II CATG 5 cut(s) 272, 359, 370, 409, 613
Kzo9I GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
LmnI GCTCC 1 cut(s) 1093
Lsp1109I GCAGC 3 cut(s) 37, 658, 806
LweI GCATC 2 cut(s) 309, 1105
MaeI CTAG 3 cut(s) 576, 807, 932
MaeIII GTNAC 4 cut(s) 724, 748, 1036, 1051
MalI GATC 7 cut(s) 5, 48, 145, 627, 649, 853, 1032
MboI GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
MboII GAAGA 7 cut(s) 257, 421, 433, 456, 965, 989, 1040
MflI RGATCY 4 cut(s) 46, 143, 647, 1030
MhlI GDGCHC 4 cut(s) 553, 583, 869, 876
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 6 cut(s) 73, 193, 344, 374, 767, 913
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 4 cut(s) 89, 446, 639, 745
MmeI TCCRAC 1 cut(s) 548
MnlI CCTC 8 cut(s) 15, 101, 633, 726, 950, 958, 998, 1075
Mox20I TGGCCA 1 cut(s) 294
MroXI GAANNNNTTC 2 cut(s) 482, 703
MscI TGGCCA 1 cut(s) 294
MseI TTAA 4 cut(s) 35, 329, 1014, 1155
MslI CAYNNNNRTG 1 cut(s) 1002
Msp20I TGGCCA 1 cut(s) 294
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 2 cut(s) 227, 870
Mva1269I GAATGC 1 cut(s) 691
MvaI CCWGG 2 cut(s) 227, 870
MwoI GCNNNNNNNGC 7 cut(s) 162, 662, 671, 794, 803, 880, 1115
NdeI CATATG 1 cut(s) 1003
NdeII GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
NlaIII CATG 5 cut(s) 272, 359, 370, 409, 613
NlaIV GGNNCC 2 cut(s) 874, 875
NmuCI GTSAC 3 cut(s) 724, 748, 1051
NspI RCATGY 1 cut(s) 613
NspV TTCGAA 1 cut(s) 959
PasI CCCWGGG 1 cut(s) 869
PctI GAATGC 1 cut(s) 691
PdmI GAANNNNTTC 2 cut(s) 482, 703
PfeI GAWTC 4 cut(s) 758, 836, 935, 956
PkrI GCNGC 3 cut(s) 27, 673, 796
PleI GAGTC 4 cut(s) 88, 446, 638, 745
PpsI GAGTC 4 cut(s) 88, 446, 638, 745
Psp6I CCWGG 2 cut(s) 225, 868
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 2 cut(s) 225, 868
PspN4I GGNNCC 2 cut(s) 874, 875
PspOMI GGGCCC 1 cut(s) 872
PspPI GGNCC 4 cut(s) 824, 872, 873, 1162
PsuI RGATCY 4 cut(s) 46, 143, 647, 1030
RsaI GTAC 1 cut(s) 737
RsaNI GTAC 1 cut(s) 736
RseI CAYNNNNRTG 1 cut(s) 1002
SaqAI TTAA 4 cut(s) 35, 329, 1014, 1155
SatI GCNGC 3 cut(s) 26, 672, 795
Sau3AI GATC 7 cut(s) 3, 46, 143, 625, 647, 851, 1030
Sau96I GGNCC 4 cut(s) 824, 872, 873, 1162
SchI GAGTC 4 cut(s) 89, 446, 639, 745
ScrFI CCNGG 2 cut(s) 227, 870
SduI GDGCHC 4 cut(s) 553, 583, 869, 876
SfaNI GCATC 2 cut(s) 309, 1105
SfcI CTRYAG 1 cut(s) 789
SfuI TTCGAA 1 cut(s) 959
SinI GGWCC 1 cut(s) 824
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 6 cut(s) 73, 193, 344, 374, 767, 913
SsiI CCGC 1 cut(s) 163
SspMI CTAG 3 cut(s) 576, 807, 932
StyD4I CCNGG 2 cut(s) 225, 868
TaaI ACNGT 4 cut(s) 90, 438, 529, 735
TaqI TCGA 5 cut(s) 6, 30, 56, 628, 959
TaqII GACCGA 1 cut(s) 131
TasI AATT 6 cut(s) 73, 193, 344, 374, 767, 913
TfiI GAWTC 4 cut(s) 758, 836, 935, 956
Tru1I TTAA 4 cut(s) 35, 329, 1014, 1155
Tru9I TTAA 4 cut(s) 35, 329, 1014, 1155
TscAI CASTG 2 cut(s) 672, 808
TseFI GTSAC 3 cut(s) 724, 748, 1051
TseI GCWGC 3 cut(s) 25, 671, 794
Tsp45I GTSAC 3 cut(s) 724, 748, 1051
TspDTI ATGAA 4 cut(s) 24, 244, 355, 750
TspGWI ACGGA 3 cut(s) 559, 623, 1055
TspRI CASTG 2 cut(s) 672, 808
VneI GTGCAC 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 4 cut(s) 73, 193, 344, 913
XceI RCATGY 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 2 cut(s) 482, 703
XspI CTAG 3 cut(s) 576, 807, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.