MD13G1246200.v1.1

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001569
Physical Location & Seq
Forward (+)
25884963 .. 25895901
10939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1246200.v1.1.491

Sequence Viewer

Length: 2883 bp
ATGGCTGTTTCCATGAGAGATTTGGATCCAGCTTTCCAGGGAGCTGGACAAAAGGATGGACTTGAAATATGGCGCATTGAAAACTTTCGCCCTGCTCCTATCCCAAAGTCCTCTTATGGCAATTTTTTTACAGGGGACTCTTATGTGATCCTGAAAACTACAGCCTCAAAAAGTGGGGCTTTGCGGCATGAGATCCATTATTGGCTTGGAAAGGATACGTCTCAGGATGAAGCTGGAGCTGCAGCCATCAAGACAGTTGAGTTAGATGCAACCCTTGGAGGACGTGCTGTCCAGTATCGTGAAGTACAGGGTCACGAAACTGCCAAGTTCCTATCTTATTTCAAACCATGTATCATACCTCAAGAAGGTGGAGTTGCATCTGGGTTCAAACATGCTGAGGCTGAAGAACACAAAACACGATTGTTTGTGTGCCAAGGAAAACATGTTGTCCATGTCAAAGAGGTTGCTTTTGCTCGATCTTCACTCAGCCATGATGACATTTTTATTTTGGATACCAAGTCTAAAATTTTTCAATTTAATGGTTCAAACTCATCTATTCAAGAAAGAGCGAAGGCGTTGGAAGTTGTCCAGTACATTAAAGACACATATCATGATGGGAAATGTGAGATAGCTTCCATTGAGGATGGAAAGTTGATGGCTGATGCAGAAAGTGGAGAATTTTGGGGTTTATTTGGTGGTTTTGCTCCACTTCCAAGGAAAACAGCCACCAATGAGGACAAAAGTTTTGATTCTTATCCTACTAAGCTTCTTTGTGTTGAGAAGGGACAGGCAGAACCAGTTGAGGGTGATGCTTTGACGAGGGATTTGCTAGACACAAACAAGTGCTATCTTCTAGATTGTGGGTTGGAAGTGTTTGTTTGGATGGGGAGAAATACATCTATTGACCAAAGAAGGAGTGCAAGTGGAGCTGCAGAAGAACTAGTCCGTCCTCCTGATCGATCGAAATCCCACATAATTCGTGTAATTGAGGGCTTTGAGACAGTAATGTTTAGGTCCAAATTCGATTCGTGGCCCCAAACAGCTGATGTAGCTGTGTCAGAAGATGGTAGGGGCAAGGTTGCAGCACTCTTAAAGCGTCAAGGGGTTAATGTGAAGGGTCTGTTGAAAGCTGATCCTGTAAAAGAAGAACCTCAACCATACATTGATTGCACTGGAAACTTGCAGGTTTGGCGTGTGAATGGCCAGGAAAAGATTAACCTTCAGTCTTCTGATCAATCAAAATTTTACAGTGGAGATTGCTATATTTTTCATTATGCATATCCAGGAGAGGATAAAGAGGAACACCTTATAGGAACATGGTTTGGAAAGCAGAGCGTTGAGGACGAAAGGGTATCAGCTATATCACTAGCAAGCAAGATGGTTGAGTCGATGAAGTTTCTTGCTGCCCAGGCTCGTATCTATGAAGGAAATGAACCAATTCAGTTCTATTCAATCTTCCAAAGCATAATTGTTTTGAAGGGTGGTCTCAGTGATGGATACAAAAATTATGTTGCGGAGAAAGAAGTTCCAGATGAGACATATCAAGAAGATGGTGTTGCATTATTTCGTGTCCAGGGATCTGGACCTGATAATATGCAAGCAATACAAGTTGATGCAGTTGCATCATCTTTGAATTCCTCCTACTGTTATATATTACATAGTGGCTCAACTGTCTTTACCTGGTCTGGAGGCCTCACAACCTCGGATGATCAGGAACTTGTTGAGAGGCAGCTCGATTTAATAAAGCCGGATCTCCAGTCGAAAATGCAAAAGGAAAATTCCGAGTCAGAACAGTTTTGGGAGTTGTTAGGAGGAAAGACCGAGTATCCCAGCCAAAAGATTGTAAGATCTGGTGAAAGCGACCCCCGCCTATTTTCTTGCACCTTCTCAAATGGAAATTTGAAGGTAGTTGAGATATACAACTTCACCCAGGATGATTTGATGACTGAAGATATATTCATCTTGGATTGTCATGCAGACATCTTTGTTTGGGTTGGCCAACAGGTCAACTCCAAGGATAGGATGCAGGCTTTAACTATTGGGGAGAAATTTCTTGAGCATGATTTTTTCATGGAACAATTATCTCGTGAAGCTTCAATATATATCATCATGGAAGGAAGTGAGCCACCTTTCTTCACACGCTTCTTTACTTGGGACTCTGCAAAATCTTCTATGCACGGGAACTCTTTCCAAAGGAAACTTACTATACTGAAAAATGGGGGTAGTCCAACTGTAAATAAACCAAAACGGAGAGCACCTGTATGTTATGGTGGAAGGTCTAGTGTACCAGAAAAATCGCAGCGTTCCAGAAGCATGTCTTTCAGCCCCGATCGAGTTCGTGTGAGGGGTAGATCTCCAGCTTTCAATGCACTGGCTGCTACTTTTGAGAATGCTAATGCCAGAAACCTTTCAACTCCACCTCCAATGGTCACAAAACTGTTCCCCAAATCTGTGACTCCAGATTCATCAAAATTGGCTTCAAAATCATCTGCTATAGCAGCACTCACTGCTAGTTTTGATAAAACAGGACCAGCAAGAGAATCTAATATACCAAGATCACCGAAAATGAGCCCAGGGCCCCCCAAGCCAAAACAACAGGAGACAAATAACAAGGAGAATTCTATGACCAGTAAACTAGAATCCTTCACCATACAGGAAGATGCGAAGGAGGGTGAAGCAGAAGATGAGAACCTCCCCGTTCACCCATATGAACGCCTTAAAACAACATCAGAAGATCCTGTTACAGATATTGATGTGACGAAGCGAGAGATTTACTTGTCAGCAGAGGAGTTCAGGGAGCATTTCGGTATGGCAAAGGATGCCTTCCATAAGTTGCCCAAGTGGAAACAGAACAAGCTTAAAATGGCCCTTCTATTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

961

Amino Acids

107.41

Weight (kDa)

5.78

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 29 - 111 7.3e-15 Gelsolin repeat
Gelsolin PF00626 149 - 216 1.9e-10 Gelsolin repeat
Gelsolin PF00626 267 - 337 1.1e-08 Gelsolin repeat
Gelsolin PF00626 635 - 712 2.4e-08 Gelsolin repeat
VHP PF02209 925 - 960 9e-14 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1174
AciI CCGC 3 cut(s) 184, 1514, 1867
AclWI GGATC 8 cut(s) 20, 33, 142, 187, 1127, 1585, 1758, 2729
AcoI YGGCCR 2 cut(s) 1201, 1996
AcsI RAATTY 9 cut(s) 525, 677, 1019, 1241, 1633, 1777, 1897, 2048, 2617
AcuI CTGAAG 3 cut(s) 423, 1205, 1968
AfaI GTAC 3 cut(s) 306, 593, 2286
AfiI CCNNNNNNNGG 3 cut(s) 365, 803, 2019
AflIII ACRYGT 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 287
AhlI ACTAGT 1 cut(s) 940
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 8 cut(s) 36, 1203, 1282, 1407, 1572, 1679, 1929, 2572
Alw21I GWGCWC 1 cut(s) 2257
Alw26I GTCTC 5 cut(s) 225, 992, 1490, 1529, 2594
AlwI GGATC 8 cut(s) 20, 33, 142, 187, 1127, 1585, 1758, 2729
AoxI GGCC 6 cut(s) 1031, 1201, 1690, 1996, 2576, 2865
ApaI GGGCCC 1 cut(s) 2580
ApeKI GCWGC 9 cut(s) 239, 242, 929, 1082, 1403, 1729, 2299, 2375, 2498
ApoI RAATTY 9 cut(s) 525, 677, 1019, 1241, 1633, 1777, 1897, 2048, 2617
ArsI GACNNNNNNTTYG 4 cut(s) 728, 760, 808, 840
Asp700I GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 7 cut(s) 1014, 1032, 1583, 2528, 2576, 2577, 2866
AsuHPI GGTGA 7 cut(s) 818, 1865, 1918, 2550, 2638, 2684, 2693
AvaII GGWCC 3 cut(s) 1014, 1583, 2528
BaeGI GKGCMC 1 cut(s) 2580
BalI TGGCCA 2 cut(s) 1203, 1998
BamHI GGATCC 1 cut(s) 25
BanII GRGCYC 2 cut(s) 2573, 2580
BauI CACGAG 1 cut(s) 2085
BbsI GAAGAC 1 cut(s) 1218
Bbv12I GWGCWC 1 cut(s) 2257
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 9 cut(s) 226, 254, 916, 1094, 1390, 1741, 2311, 2362, 2510
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BciT130I CCWGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
BciVI GTATCC 4 cut(s) 208, 505, 1490, 1836
BclI TGATCA 2 cut(s) 1231, 1708
BcoDI GTCTC 5 cut(s) 225, 992, 1490, 1529, 2594
BcuI ACTAGT 1 cut(s) 940
BfaI CTAG 7 cut(s) 830, 854, 941, 1367, 2280, 2511, 2636
BfmI CTRYAG 4 cut(s) 159, 240, 930, 2493
BfuAI ACCTGC 1 cut(s) 1174
BfuI GTATCC 4 cut(s) 208, 505, 1490, 1836
BglII AGATCT 2 cut(s) 1847, 2351
Bme1390I CCNGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
Bme18I GGWCC 3 cut(s) 1014, 1583, 2528
BmeRI GACNNNNNGTC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 7 cut(s) 1014, 1032, 1583, 2528, 2576, 2577, 2866
BmiI GGNNCC 4 cut(s) 27, 1034, 2578, 2579
BmrFI CCNGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
BmsI GCATC 9 cut(s) 256, 386, 652, 799, 1603, 1631, 2013, 2650, 2809
BpiI GAAGAC 1 cut(s) 1218
BpmI CTGGAG 5 cut(s) 255, 1707, 1739, 2340, 2442
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 345, 2075
Bsa29I ATCGAT 1 cut(s) 958
BsaBI GATNNNNATC 3 cut(s) 24, 753, 964
BsaI GGTCTC 1 cut(s) 1490
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 2404, 2434
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 803, 2019
Bse1I ACTGG 7 cut(s) 292, 589, 797, 1177, 1756, 2376, 2628
Bse8I GATNNNNATC 3 cut(s) 24, 753, 964
BseBI CCWGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
BseCI ATCGAT 1 cut(s) 958
BseGI GGATG 8 cut(s) 61, 232, 649, 888, 1711, 1939, 2028, 2824
BseJI GATNNNNATC 3 cut(s) 24, 753, 964
BseLI CCNNNNNNNGG 3 cut(s) 365, 803, 2019
BseMII CTCAG 4 cut(s) 236, 387, 499, 1501
BseNI ACTGG 7 cut(s) 292, 589, 797, 1177, 1756, 2376, 2628
BseRI GAGGAG 1 cut(s) 2801
BseSI GKGCMC 1 cut(s) 2580
BseXI GCAGC 9 cut(s) 226, 254, 916, 1094, 1390, 1741, 2311, 2362, 2510
BseYI CCCAGC 1 cut(s) 1829
Bsh1285I CGRYCG 2 cut(s) 962, 2332
BshFI GGCC 6 cut(s) 1033, 1203, 1692, 1998, 2578, 2867
BshVI ATCGAT 1 cut(s) 958
BsiEI CGRYCG 2 cut(s) 962, 2332
BsiHKAI GWGCWC 1 cut(s) 2257
BsiSI CCGG 1 cut(s) 1748
BslFI GGGAC 3 cut(s) 149, 798, 2168
BslI CCNNNNNNNGG 3 cut(s) 365, 803, 2019
BsmAI GTCTC 5 cut(s) 225, 992, 1490, 1529, 2594
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 3 cut(s) 149, 798, 2168
BsmI GAATGC 1 cut(s) 2395
BsnI GGCC 6 cut(s) 1033, 1203, 1692, 1998, 2578, 2867
Bso31I GGTCTC 1 cut(s) 1490
Bsp120I GGGCCC 1 cut(s) 2576
Bsp1286I GDGCHC 3 cut(s) 2257, 2573, 2580
BspACI CCGC 3 cut(s) 184, 1514, 1867
BspANI GGCC 6 cut(s) 1033, 1203, 1692, 1998, 2578, 2867
BspCNI CTCAG 4 cut(s) 235, 388, 498, 1500
BspDI ATCGAT 1 cut(s) 958
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 4 cut(s) 27, 1034, 2578, 2579
BspMAI CTGCAG 2 cut(s) 244, 934
BspMI ACCTGC 1 cut(s) 1174
BspPI GGATC 8 cut(s) 20, 33, 142, 187, 1127, 1585, 1758, 2729
BspTNI GGTCTC 1 cut(s) 1490
BsrI ACTGG 7 cut(s) 292, 589, 797, 1177, 1756, 2376, 2628
BssSI CACGAG 1 cut(s) 2085
BssT1I CCWWGG 4 cut(s) 274, 433, 713, 2013
Bst2BI CACGAG 1 cut(s) 2085
Bst2UI CCWGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
Bst4CI ACNGT 8 cut(s) 256, 1003, 1250, 1646, 1672, 1794, 2233, 2439
BstAPI GCANNNNNTGC 3 cut(s) 2375, 2507, 2819
BstC8I GCNNGC 3 cut(s) 1372, 1599, 2028
BstDEI CTNAG 5 cut(s) 222, 396, 485, 762, 1487
BstENI CCTNNNNNAGG 1 cut(s) 363
BstF5I GGATG 8 cut(s) 61, 232, 649, 888, 1711, 1939, 2028, 2824
BstHHI GCGC 1 cut(s) 75
BstMAI GTCTC 5 cut(s) 225, 992, 1490, 1529, 2594
BstMCI CGRYCG 2 cut(s) 962, 2332
BstNI CCWGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
BstNSI RCATGY 3 cut(s) 395, 446, 2317
BstSCI CCNGG 8 cut(s) 36, 1203, 1282, 1407, 1572, 1679, 1929, 2572
BstSFI CTRYAG 4 cut(s) 159, 240, 930, 2493
BstSLI GKGCMC 1 cut(s) 2580
BstV1I GCAGC 9 cut(s) 226, 254, 916, 1094, 1390, 1741, 2311, 2362, 2510
BstV2I GAAGAC 1 cut(s) 1218
BstX2I RGATCY 7 cut(s) 25, 192, 1577, 1750, 1847, 2351, 2734
BstXI CCANNNNNNTGG 2 cut(s) 44, 1580
BstYI RGATCY 7 cut(s) 25, 192, 1577, 1750, 1847, 2351, 2734
Bsu15I ATCGAT 1 cut(s) 958
BsuI GTATCC 4 cut(s) 208, 505, 1490, 1836
BsuRI GGCC 6 cut(s) 1033, 1203, 1692, 1998, 2578, 2867
BsuTUI ATCGAT 1 cut(s) 958
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 8 cut(s) 61, 232, 649, 888, 1711, 1939, 2028, 2824
BtsI GCAGTG 1 cut(s) 2505
BtsIMutI CAGTG 5 cut(s) 1170, 1255, 1495, 2369, 2505
BveI ACCTGC 1 cut(s) 1174
Cac8I GCNNGC 3 cut(s) 1372, 1599, 2028
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 7 cut(s) 1014, 1032, 1583, 2528, 2576, 2577, 2866
ClaI ATCGAT 1 cut(s) 958
CseI GACGC 1 cut(s) 1085
CsiI ACCWGGT 1 cut(s) 1679
Csp6I GTAC 3 cut(s) 305, 592, 2285
CviQI GTAC 3 cut(s) 305, 592, 2285
DdeI CTNAG 5 cut(s) 222, 396, 485, 762, 1487
DriI GACNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 2 cut(s) 1201, 1996
Eam1105I GACNNNNNGTC 1 cut(s) 287
Eco130I CCWWGG 4 cut(s) 274, 433, 713, 2013
Eco147I AGGCCT 1 cut(s) 1692
Eco24I GRGCYC 2 cut(s) 2573, 2580
Eco31I GGTCTC 1 cut(s) 1490
Eco47I GGWCC 3 cut(s) 1014, 1583, 2528
Eco57I CTGAAG 3 cut(s) 423, 1205, 1968
EcoNI CCTNNNNNAGG 1 cut(s) 363
EcoO109I RGGNCCY 2 cut(s) 2576, 2577
EcoRI GAATTC 2 cut(s) 1633, 2617
EcoRII CCWGG 8 cut(s) 36, 1203, 1282, 1407, 1572, 1679, 1929, 2572
EcoT14I CCWWGG 4 cut(s) 274, 433, 713, 2013
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 2 cut(s) 2573, 2580
ErhI CCWWGG 4 cut(s) 274, 433, 713, 2013
Esp3I CGTCTC 1 cut(s) 225
FalI AAGNNNNNCTT 6 cut(s) 163, 195, 2302, 2334, 2807, 2839
FaqI GGGAC 3 cut(s) 149, 798, 2168
FauI CCCGC 1 cut(s) 1874
FauNDI CATATG 1 cut(s) 2707
FbaI TGATCA 2 cut(s) 1231, 1708
FokI GGATG 8 cut(s) 68, 239, 656, 895, 1718, 1946, 2035, 2831
FriOI GRGCYC 2 cut(s) 2573, 2580
FspBI CTAG 7 cut(s) 830, 854, 941, 1367, 2280, 2511, 2636
GlaI GCGC 1 cut(s) 74
GsaI CCCAGC 1 cut(s) 1833
GsuI CTGGAG 5 cut(s) 255, 1707, 1739, 2340, 2442
HaeIII GGCC 6 cut(s) 1033, 1203, 1692, 1998, 2578, 2867
HapII CCGG 1 cut(s) 1748
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 1 cut(s) 75
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 1 cut(s) 2008
HindII GTYRAC 1 cut(s) 2008
HindIII AAGCTT 3 cut(s) 764, 2091, 2855
HpaII CCGG 1 cut(s) 1748
HphI GGTGA 7 cut(s) 818, 1865, 1918, 2550, 2638, 2684, 2693
Hpy166II GTNNAC 4 cut(s) 2008, 2285, 2633, 2701
Hpy188I TCNGA 7 cut(s) 1060, 1231, 1705, 1783, 1789, 2731, 2882
Hpy8I GTNNAC 4 cut(s) 2008, 2285, 2633, 2701
HpyCH4III ACNGT 8 cut(s) 256, 1003, 1250, 1646, 1672, 1794, 2233, 2439
HpyCH4IV ACGT 2 cut(s) 218, 283
HpyF3I CTNAG 5 cut(s) 222, 396, 485, 762, 1487
HpySE526I ACGT 2 cut(s) 218, 283
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 2 cut(s) 1231, 1708
LmnI GCTCC 6 cut(s) 41, 100, 236, 709, 926, 2797
Lsp1109I GCAGC 9 cut(s) 226, 254, 916, 1094, 1390, 1741, 2311, 2362, 2510
LweI GCATC 9 cut(s) 256, 386, 652, 799, 1603, 1631, 2013, 2650, 2809
MabI ACCWGGT 1 cut(s) 1679
MaeI CTAG 7 cut(s) 830, 854, 941, 1367, 2280, 2511, 2636
MaeII ACGT 2 cut(s) 218, 283
MaeIII GTNAC 5 cut(s) 311, 2428, 2452, 2740, 2755
MflI RGATCY 7 cut(s) 25, 192, 1577, 1750, 1847, 2351, 2734
MhlI GDGCHC 3 cut(s) 2257, 2573, 2580
MlsI TGGCCA 2 cut(s) 1203, 1998
MluNI TGGCCA 2 cut(s) 1203, 1998
MlyI GAGTC 5 cut(s) 131, 1394, 1793, 2150, 2449
MmeI TCCRAC 3 cut(s) 558, 846, 2252
Mox20I TGGCCA 2 cut(s) 1203, 1998
Mph1103I ATGCAT 1 cut(s) 1279
MroXI GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
MscI TGGCCA 2 cut(s) 1203, 1998
MseI TTAA 9 cut(s) 537, 597, 1091, 1107, 1215, 1739, 2033, 2718, 2859
MslI CAYNNNNRTG 2 cut(s) 2260, 2706
Msp20I TGGCCA 2 cut(s) 1203, 1998
MspA1I CMGCKG 1 cut(s) 1043
MspI CCGG 1 cut(s) 1748
MspR9I CCNGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
Mva1269I GAATGC 1 cut(s) 2395
MvaI CCWGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
NdeI CATATG 1 cut(s) 2707
NlaIV GGNNCC 4 cut(s) 27, 1034, 2578, 2579
NmuCI GTSAC 4 cut(s) 311, 2428, 2452, 2755
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 3 cut(s) 395, 446, 2317
PagI TCATGA 1 cut(s) 610
PasI CCCWGGG 1 cut(s) 2573
PceI AGGCCT 1 cut(s) 1692
PciI ACATGT 1 cut(s) 442
PctI GAATGC 1 cut(s) 2395
PdmI GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
PfeI GAWTC 5 cut(s) 749, 1025, 2462, 2540, 2639
PfoI TCCNGGA 1 cut(s) 1282
Ple19I CGATCG 2 cut(s) 962, 2332
PleI GAGTC 5 cut(s) 131, 1393, 1792, 2150, 2449
PpsI GAGTC 5 cut(s) 131, 1393, 1792, 2150, 2449
PscI ACATGT 1 cut(s) 442
Psp6I CCWGG 8 cut(s) 36, 1203, 1282, 1407, 1572, 1679, 1929, 2572
PspFI CCCAGC 1 cut(s) 1829
PspGI CCWGG 8 cut(s) 36, 1203, 1282, 1407, 1572, 1679, 1929, 2572
PspN4I GGNNCC 4 cut(s) 27, 1034, 2578, 2579
PspOMI GGGCCC 1 cut(s) 2576
PspPI GGNCC 7 cut(s) 1014, 1032, 1583, 2528, 2576, 2577, 2866
PstI CTGCAG 2 cut(s) 244, 934
PsuI RGATCY 7 cut(s) 25, 192, 1577, 1750, 1847, 2351, 2734
PvuI CGATCG 2 cut(s) 962, 2332
PvuII CAGCTG 1 cut(s) 1043
RsaI GTAC 3 cut(s) 306, 593, 2286
RsaNI GTAC 3 cut(s) 305, 592, 2285
RseI CAYNNNNRTG 2 cut(s) 2260, 2706
SaqAI TTAA 9 cut(s) 537, 597, 1091, 1107, 1215, 1739, 2033, 2718, 2859
Sau96I GGNCC 7 cut(s) 1014, 1032, 1583, 2528, 2576, 2577, 2866
SchI GAGTC 5 cut(s) 131, 1394, 1793, 2150, 2449
ScrFI CCNGG 8 cut(s) 38, 1205, 1284, 1409, 1574, 1681, 1931, 2574
SduI GDGCHC 3 cut(s) 2257, 2573, 2580
SexAI ACCWGGT 1 cut(s) 1679
SfaNI GCATC 9 cut(s) 256, 386, 652, 799, 1603, 1631, 2013, 2650, 2809
SfcI CTRYAG 4 cut(s) 159, 240, 930, 2493
SinI GGWCC 3 cut(s) 1014, 1583, 2528
SmiMI CAYNNNNRTG 2 cut(s) 2260, 2706
SmlI CTYRAG 2 cut(s) 360, 2054
SmoI CTYRAG 2 cut(s) 360, 2054
SpeI ACTAGT 1 cut(s) 940
SseBI AGGCCT 1 cut(s) 1692
SsiI CCGC 3 cut(s) 184, 1514, 1867
SspMI CTAG 7 cut(s) 830, 854, 941, 1367, 2280, 2511, 2636
StuI AGGCCT 1 cut(s) 1692
StyD4I CCNGG 8 cut(s) 36, 1203, 1282, 1407, 1572, 1679, 1929, 2572
StyI CCWWGG 4 cut(s) 274, 433, 713, 2013
TaaI ACNGT 8 cut(s) 256, 1003, 1250, 1646, 1672, 1794, 2233, 2439
TaiI ACGT 2 cut(s) 221, 286
TaqI TCGA 8 cut(s) 475, 958, 962, 1023, 1388, 1734, 1760, 2332
TaqII GACCGA 1 cut(s) 1835
TatI WGTACW 2 cut(s) 304, 591
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 5 cut(s) 749, 1025, 2462, 2540, 2639
Tru1I TTAA 9 cut(s) 537, 597, 1091, 1107, 1215, 1739, 2033, 2718, 2859
Tru9I TTAA 9 cut(s) 537, 597, 1091, 1107, 1215, 1739, 2033, 2718, 2859
TscAI CASTG 5 cut(s) 1177, 1255, 1495, 2376, 2512
TseFI GTSAC 4 cut(s) 311, 2428, 2452, 2755
TseI GCWGC 9 cut(s) 239, 242, 929, 1082, 1403, 1729, 2299, 2375, 2498
Tsp45I GTSAC 4 cut(s) 311, 2428, 2452, 2755
TspDTI ATGAA 9 cut(s) 243, 1259, 1406, 1437, 1446, 1948, 2059, 2454, 2724
TspGWI ACGGA 2 cut(s) 935, 2263
TspRI CASTG 5 cut(s) 1177, 1255, 1495, 2376, 2512
VpaK11BI GGWCC 3 cut(s) 1014, 1583, 2528
XagI CCTNNNNNAGG 1 cut(s) 363
XapI RAATTY 9 cut(s) 525, 677, 1019, 1241, 1633, 1777, 1897, 2048, 2617
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 3 cut(s) 395, 446, 2317
XcmI CCANNNNNNNNNTGG 2 cut(s) 19, 203
XmnI GAANNNNTTC 4 cut(s) 84, 1437, 2186, 2407
XspI CTAG 7 cut(s) 830, 854, 941, 1367, 2280, 2511, 2636
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.