FvH4_4g07730

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001569
Physical Location & Seq
Forward (+)
7192939 .. 7204486
11548 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07730.t3

Sequence Viewer

Length: 2865 bp
ATGGCTGTTTCCATGAGAGATTTGGATCCAGCTTTCCACGGAGCTGGACAAAAGGCTGGACTGGAAATATGGCGCATTGAAAACTTTTGTCCTGTTAGTGTCCCACAATCCTCTCATGGAAAGTTTTTTATGGGCGACTCTTATGTGATCTTGAAAACAACAGCCTCAAAGAGTGGTGCCTTGCGACATGATATCCATTATTGGCTTGGTAAAGATACTTCTCAGGATGAAGCAGGCACTGCAGCCATCAAGACAGTTGAATTAGATGCAGCTCTTGGAGGACGTGCTGTTCAGTATCGTGAAGTACAGGGTCACGAAACTGCTAAGTTTCTATCTTATTTTAAACCGTGTATCATACCTCAAGAAGGTGGAATTGCATCTGGGTTTAAACATGCTGAGGCTGAAGAACACACAACACGGTTGTATGTTTGCAAAGGAAAACATGTCGTCCATGTCAAAGAGGTTTCTTTTGCTCGATCTTCACTCAACCATGACGACATTTTTATTCTGGACACCAAGTCTAAAATTTTTCAGTTTAATGGTTCCAACTCATCCATTCAAGAAAGGGCAAAGGCTTTAGAAGTTGTTCAGTACATTAAAGACACGTATCACGACGGAAGGTGTGACATAGCTTCCATTGAGGACGGAAAGTTGATGGCGGATGCTGATACTGGAGAATTCTGGGCCTTATTTGGTGGCTTTGCTCCACTTCCGAGGAAAACAGCCAATGATGAAGATAAAATTTTTGATTCTCATACTACTACGCTACTTCGCGTTGATAAAGGGAAGGCAGAACCGGTTGGGGCAGATTCTCTGACAAGGGAATTACTAGAAACAAGTAAATGCTATCTGTTAGACTGTGGGCTGGAAGTGTTTGTTTGGATGGGGAGAAACACATCTCTTGATGAGAGGAGGAGTGCAAGTGAAGCTGCAGAAGAGTTGGTACGTGGTCCTGATCGGTCAAAATCCCACATGATTCGTGTTATTGAGGGATTTGAGACTGTAATGTTTAAGTCCAAATTCGATACATGGCCTGAAGCAGTTGAAGTAGCAGTGTCAGAGGATGGTAGGGGCAAGGTTGCAGCACTTCTGAAGCGTCAAGGGGTTAATGTGAAGGGTCTGTTGAAAGCTGATCCTGTAAAAGAAGAACCTCAACCATACATTGATTGCACAGGAAATCTGCAGGTTTGGCGAGTGAATGGCCAGGAAAAGATTCTTCTTCCTGCTTCTGATCAATCAAAAATTTATAGCGGAGATTGCTATATCTTCCAGTACTCGTATCCAGGAGAAGATAAAGAAGAACACCTGATAGGAACATGGTTTGGAAAACAGAGTGTTGAGGAAGAAAGAGCATCAGCTATTTCACTTGCAAGCAACATGGTCGCATCAATGAAGTTTCTGCCAGCGCAGGCTCGCATCTATGAAGGAAAGGAACCAATTCAGTTCTATTCAATCTTCCAAAGCTTAATTGTTCTGAAGGGCGGTCTAAGTGATGGCTACAAAAAGTATGTTGCGGAGAAGGAAGTTCCAGATGACACATATCAAGAAGATGGTGTTGCTTTATTTCGTGTCCAGGGATCTGGACCTGATAATATGCAAGCAATTCAAGTTGAAGCAGTTGCATCATCTTTGAATTCCGCCTACTGTTATATATTACACAGTGGCTCAACTGTCTTTACATGGTCTGGAAGCCTTGCAACCACAGATGACCAGGAACTTGTTGAGAGGCAGCTGGATCTAATAAAGCCAAATCTTCAGACGAAACCACAAAAGGAAAATTCCGAATCTGAACAGTTTTGGGATTTGTTAGGAGCAAAGGCCGAGTATTCCGGCCAAAAGATTGTAAGAGATGCAGAAAGTGATCCTCGCCTATTTTCCTGTGTTTTCTCAAATGAGAATCTAAAGGTAGTGGAGATATATAATTTCACCCAGGATGATTTGATGACTGAAGATATATTCATCTTGGATTGTCACTCTGATATCTTTGTTTGGGTTGGCGAAGAAGTCAACTCCAAAGATAAAATGCATGCTTTAACTATCGGGGAGAAATTTCTTGAGCGTGATTTTCTTATGGAAAAATTATCTCATGAAGCTCCAATATATATCATCATGGAAGGAAGTGAGCCACCTTTCTTCACACGCTTCTTCACCTGGGACTCTGCAAAGTCTAATATGCATGGAAATTCATTCCAACGAAAACTTACTATAGTGAAGCATGGGCGATCTCCAGTTGTAGATAAACCCAAACGAAGAACACCCGTATCTTATGGTGGGAGGTCTAGTGTGCCAGAAAAATCACAGCGTTCCAGAAGCATGTCTTTCAGCCCTGACCGAGTACGTGTTAGGGGCAGGTCTCCAGCTTTCAATGCACTGGCTGCTACTTTTGAGAGTAATAATGCGAGGAACCTTTCAACTCCACCCCCAATGGTTAGAAAATCCCAACTGTATCCAAAATCTGTGACCCCAGATACATCAAAAGTGGCTTCAAAATCATCAGCTATAGCAGCACTCACTGCTGGATTTGAAAAAAAAGAAAACAATATACCAAGATCACCTAAAGGACCTCCCAAAGCAAAATCAGAGACAAATAACAAGGAGACTTGTATGGGCAGCAAAATGGAATCCCTCACGATAGAGGAAGATGTAAAAGAGGGTGAAGCAGAAGACGAAGGTCTTCCTGTTCACCCATATCAACGCGTTAAAACAACATCAACAGATCCTGTTGCAGATATTGATGTGACAAAACGAGAGATTTACTTGTCATCAGAAGAGTTCAGGGAGCATCTTGGGATGGCAAAGGATGCTTTCTATAAGTTACCCAAGTGGAAGCAGAACAAGCTTAAAATGGCCGTTCAACTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

955

Amino Acids

106.78

Weight (kDa)

5.87

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 32 - 111 1.1e-13 Gelsolin repeat
Gelsolin PF00626 149 - 208 2.2e-10 Gelsolin repeat
Gelsolin PF00626 267 - 317 1.8e-08 Gelsolin repeat
Gelsolin PF00626 635 - 712 5e-09 Gelsolin repeat
VHP PF02209 919 - 954 4.4e-15 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1174, 2340
AccB1I GGYRCC 1 cut(s) 176
AccII CGCG 2 cut(s) 774, 2697
AciI CCGC 5 cut(s) 659, 1251, 1482, 1514, 1638
AclWI GGATC 7 cut(s) 20, 33, 1127, 1585, 1743, 1856, 2711
AcoI YGGCCR 3 cut(s) 1201, 1831, 2847
AcsI RAATTY 9 cut(s) 525, 677, 741, 1019, 1242, 1633, 1777, 2048, 2182
AcuI CTGAAG 6 cut(s) 423, 1056, 1112, 1496, 1739, 1968
AfaI GTAC 5 cut(s) 306, 593, 945, 1274, 2337
AfiI CCNNNNNNNGG 1 cut(s) 365
AflIII ACRYGT 4 cut(s) 442, 603, 2338, 2695
AgeI ACCGGT 1 cut(s) 796
AhdI GACNNNNNGTC 1 cut(s) 517
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 6 cut(s) 1203, 1282, 1572, 1710, 1929, 2150
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
Alw26I GTCTC 4 cut(s) 992, 2358, 2576, 2591
AlwI GGATC 7 cut(s) 20, 33, 1127, 1585, 1743, 1856, 2711
AlwNI CAGNNNCTG 2 cut(s) 239, 2720
AoxI GGCC 6 cut(s) 684, 1031, 1201, 1818, 1831, 2847
ApeKI GCWGC 8 cut(s) 242, 269, 929, 1082, 1729, 2375, 2504, 2610
ApoI RAATTY 9 cut(s) 525, 677, 741, 1019, 1242, 1633, 1777, 2048, 2182
AsiGI ACCGGT 1 cut(s) 796
Asp700I GAANNNNTTC 4 cut(s) 585, 1212, 1437, 2673
AspLEI GCGC 2 cut(s) 75, 1408
AspS9I GGNCC 4 cut(s) 684, 950, 1583, 2561
AsuHPI GGTGA 5 cut(s) 1918, 2140, 2544, 2666, 2675
AvaII GGWCC 3 cut(s) 950, 1583, 2561
BaeI ACNNNNGTAYC 2 cut(s) 2244, 2277
BalI TGGCCA 1 cut(s) 1203
BamHI GGATCC 1 cut(s) 25
BanI GGYRCC 1 cut(s) 176
BarI GAAGNNNNNNTAC 4 cut(s) 202, 234, 927, 959
BbsI GAAGAC 2 cut(s) 2666, 2670
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2622
BccI CCATC 7 cut(s) 254, 649, 877, 1058, 1487, 1544, 2785
BceAI ACGGC 1 cut(s) 2834
BcgI CGANNNNNNTGC 2 cut(s) 1363, 1397
BciT130I CCWGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
BciVI GTATCC 2 cut(s) 1290, 2457
BclI TGATCA 1 cut(s) 1231
BcoDI GTCTC 4 cut(s) 992, 2358, 2576, 2591
BfaI CTAG 2 cut(s) 830, 2280
BfmI CTRYAG 5 cut(s) 240, 930, 1181, 2205, 2499
BfuAI ACCTGC 2 cut(s) 1174, 2340
BfuI GTATCC 2 cut(s) 1290, 2457
BisI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2611
BlsI GCNGC 8 cut(s) 244, 271, 931, 1084, 1731, 2377, 2506, 2612
BmcAI AGTACT 1 cut(s) 1274
Bme1390I CCNGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
Bme18I GGWCC 3 cut(s) 950, 1583, 2561
BmeRI GACNNNNNGTC 1 cut(s) 517
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 4 cut(s) 684, 950, 1583, 2561
BmiI GGNNCC 5 cut(s) 27, 178, 544, 1434, 2405
BmrFI CCNGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
BoxI GACNNNNGTC 1 cut(s) 2670
BpiI GAAGAC 2 cut(s) 2666, 2670
BpmI CTGGAG 3 cut(s) 693, 2211, 2340
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 345, 2075
BsaAI YACGTR 3 cut(s) 606, 947, 2339
BsaBI GATNNNNATC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 2358
BsaJI CCNNGG 5 cut(s) 37, 713, 1573, 1929, 2151
BsaWI WCCGGW 1 cut(s) 796
BsaXI ACNNNNNCTCC 4 cut(s) 907, 937, 2266, 2296
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse118I RCCGGY 1 cut(s) 796
Bse1I ACTGG 5 cut(s) 66, 676, 1270, 2228, 2376
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
BseDI CCNNGG 5 cut(s) 37, 713, 1573, 1929, 2151
BseGI GGATG 8 cut(s) 232, 551, 667, 888, 1069, 1939, 2796, 2806
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMII CTCAG 2 cut(s) 236, 387
BseNI ACTGG 5 cut(s) 66, 676, 1270, 2228, 2376
BseRI GAGGAG 2 cut(s) 925, 928
BseXI GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2622
Bsh1236I CGCG 2 cut(s) 774, 2697
BshFI GGCC 6 cut(s) 686, 1033, 1203, 1820, 1833, 2849
BshNI GGYRCC 1 cut(s) 176
BshTI ACCGGT 1 cut(s) 796
BsiSI CCGG 2 cut(s) 797, 1830
BslFI GGGAC 2 cut(s) 86, 2168
BslI CCNNNNNNNGG 1 cut(s) 365
BsmAI GTCTC 4 cut(s) 992, 2358, 2576, 2591
BsmFI GGGAC 2 cut(s) 86, 2168
BsnI GGCC 6 cut(s) 686, 1033, 1203, 1820, 1833, 2849
Bso31I GGTCTC 1 cut(s) 2358
BspACI CCGC 5 cut(s) 659, 1251, 1482, 1514, 1638
BspANI GGCC 6 cut(s) 686, 1033, 1203, 1820, 1833, 2849
BspCNI CTCAG 2 cut(s) 235, 388
BspFNI CGCG 2 cut(s) 774, 2697
BspHI TCATGA 1 cut(s) 2086
BspLI GGNNCC 5 cut(s) 27, 178, 544, 1434, 2405
BspMAI CTGCAG 3 cut(s) 244, 934, 1185
BspMI ACCTGC 2 cut(s) 1174, 2340
BspPI GGATC 7 cut(s) 20, 33, 1127, 1585, 1743, 1856, 2711
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 2358
BsrFI RCCGGY 1 cut(s) 796
BsrI ACTGG 5 cut(s) 66, 676, 1270, 2228, 2376
BssAI RCCGGY 1 cut(s) 796
BssECI CCNNGG 5 cut(s) 37, 713, 1573, 1929, 2151
Bst2UI CCWGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
Bst6I CTCTTC 2 cut(s) 930, 2763
BstAPI GCANNNNNTGC 4 cut(s) 239, 2375, 2513, 2801
BstBAI YACGTR 3 cut(s) 606, 947, 2339
BstC8I GCNNGC 7 cut(s) 235, 1372, 1404, 1410, 1414, 1599, 2028
BstDEI CTNAG 4 cut(s) 222, 324, 396, 1487
BstDSI CCRYGG 1 cut(s) 37
BstENI CCTNNNNNAGG 1 cut(s) 363
BstF5I GGATG 8 cut(s) 232, 551, 667, 888, 1069, 1939, 2796, 2806
BstFNI CGCG 2 cut(s) 774, 2697
BstHHI GCGC 2 cut(s) 75, 1408
BstMAI GTCTC 4 cut(s) 992, 2358, 2576, 2591
BstNI CCWGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
BstNSI RCATGY 4 cut(s) 395, 446, 2030, 2317
BstPAI GACNNNNGTC 1 cut(s) 2670
BstSCI CCNGG 6 cut(s) 1203, 1282, 1572, 1710, 1929, 2150
BstSFI CTRYAG 5 cut(s) 240, 930, 1181, 2205, 2499
BstUI CGCG 2 cut(s) 774, 2697
BstV1I GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2622
BstV2I GAAGAC 2 cut(s) 2666, 2670
BstX2I RGATCY 4 cut(s) 25, 1577, 1735, 2716
BstXI CCANNNNNNTGG 2 cut(s) 44, 1580
BstYI RGATCY 4 cut(s) 25, 1577, 1735, 2716
BsuI GTATCC 2 cut(s) 1290, 2457
BsuRI GGCC 6 cut(s) 686, 1033, 1203, 1820, 1833, 2849
BtgI CCRYGG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 8 cut(s) 232, 551, 667, 888, 1069, 1939, 2796, 2806
BtsI GCAGTG 3 cut(s) 237, 1059, 2511
BtsIMutI CAGTG 5 cut(s) 237, 1059, 1666, 2369, 2511
BveI ACCTGC 2 cut(s) 1174, 2340
Cac8I GCNNGC 7 cut(s) 235, 1372, 1404, 1410, 1414, 1599, 2028
CaiI CAGNNNCTG 2 cut(s) 239, 2720
CciI TCATGA 1 cut(s) 2086
CfoI GCGC 2 cut(s) 75, 1408
Cfr10I RCCGGY 1 cut(s) 796
Cfr13I GGNCC 4 cut(s) 684, 950, 1583, 2561
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 5 cut(s) 305, 592, 944, 1273, 2336
CspAI ACCGGT 1 cut(s) 796
CviQI GTAC 5 cut(s) 305, 592, 944, 1273, 2336
DdeI CTNAG 4 cut(s) 222, 324, 396, 1487
DraI TTTAAA 2 cut(s) 343, 388
DriI GACNNNNNGTC 1 cut(s) 517
EaeI YGGCCR 3 cut(s) 1201, 1831, 2847
Eam1104I CTCTTC 2 cut(s) 930, 2763
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 2 cut(s) 930, 2763
EciI GGCGGA 2 cut(s) 674, 1627
Eco31I GGTCTC 1 cut(s) 2358
Eco32I GATATC 2 cut(s) 193, 1981
Eco47I GGWCC 3 cut(s) 950, 1583, 2561
Eco57I CTGAAG 6 cut(s) 423, 1056, 1112, 1496, 1739, 1968
EcoNI CCTNNNNNAGG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 2561
EcoRI GAATTC 2 cut(s) 677, 1633
EcoRII CCWGG 6 cut(s) 1203, 1282, 1572, 1710, 1929, 2150
EcoRV GATATC 2 cut(s) 193, 1981
EcoT22I ATGCAT 2 cut(s) 2028, 2178
FalI AAGNNNNNCTT 2 cut(s) 2302, 2334
FaqI GGGAC 2 cut(s) 86, 2168
FbaI TGATCA 1 cut(s) 1231
Fnu4HI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2611
FokI GGATG 8 cut(s) 239, 538, 674, 895, 1076, 1946, 2803, 2813
Fsp4HI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2611
FspBI CTAG 2 cut(s) 830, 2280
GlaI GCGC 2 cut(s) 74, 1407
GluI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2611
GsuI CTGGAG 3 cut(s) 693, 2211, 2340
HaeIII GGCC 6 cut(s) 686, 1033, 1203, 1820, 1833, 2849
HapII CCGG 2 cut(s) 797, 1830
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 2 cut(s) 75, 1408
Hin6I GCGC 2 cut(s) 73, 1406
HinP1I GCGC 2 cut(s) 73, 1406
HincII GTYRAC 1 cut(s) 2008
HindII GTYRAC 1 cut(s) 2008
HindIII AAGCTT 2 cut(s) 1462, 2837
HinfI GANTC 9 cut(s) 137, 749, 809, 976, 1213, 1784, 1897, 2156, 2621
HpaII CCGG 2 cut(s) 797, 1830
HphI GGTGA 5 cut(s) 1918, 2140, 2544, 2666, 2675
Hpy166II GTNNAC 2 cut(s) 2008, 2683
Hpy8I GTNNAC 2 cut(s) 2008, 2683
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 9 cut(s) 359, 612, 781, 1108, 1418, 1471, 1513, 2108, 2663
HpyCH4IV ACGT 4 cut(s) 283, 605, 946, 2338
HpyF3I CTNAG 4 cut(s) 222, 324, 396, 1487
HpySE526I ACGT 4 cut(s) 283, 605, 946, 2338
HspAI GCGC 2 cut(s) 73, 1406
Ksp22I TGATCA 1 cut(s) 1231
LmnI GCTCC 5 cut(s) 41, 709, 1811, 2098, 2779
Lsp1109I GCAGC 8 cut(s) 254, 281, 916, 1094, 1741, 2362, 2516, 2622
MaeI CTAG 2 cut(s) 830, 2280
MaeII ACGT 4 cut(s) 283, 605, 946, 2338
MaeIII GTNAC 6 cut(s) 311, 623, 1970, 2458, 2737, 2814
MflI RGATCY 4 cut(s) 25, 1577, 1735, 2716
MlsI TGGCCA 1 cut(s) 1203
MluI ACGCGT 1 cut(s) 2695
MluNI TGGCCA 1 cut(s) 1203
MlyI GAGTC 2 cut(s) 131, 2150
MmeI TCCRAC 2 cut(s) 570, 2215
Mox20I TGGCCA 1 cut(s) 1203
Mph1103I ATGCAT 2 cut(s) 2028, 2178
MroXI GAANNNNTTC 4 cut(s) 585, 1212, 1437, 2673
MscI TGGCCA 1 cut(s) 1203
Msp20I TGGCCA 1 cut(s) 1203
MspA1I CMGCKG 1 cut(s) 1732
MspI CCGG 2 cut(s) 797, 1830
MspR9I CCNGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
MssI GTTTAAAC 1 cut(s) 388
MvaI CCWGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
MvnI CGCG 2 cut(s) 774, 2697
NlaIV GGNNCC 5 cut(s) 27, 178, 544, 1434, 2405
NmeAIII GCCGAG 1 cut(s) 1846
NmuCI GTSAC 5 cut(s) 311, 623, 1970, 2458, 2737
NsiI ATGCAT 2 cut(s) 2028, 2178
NspI RCATGY 4 cut(s) 395, 446, 2030, 2317
PaeI GCATGC 1 cut(s) 2030
PagI TCATGA 1 cut(s) 2086
PciI ACATGT 1 cut(s) 442
PdmI GAANNNNTTC 4 cut(s) 585, 1212, 1437, 2673
PfeI GAWTC 7 cut(s) 749, 809, 976, 1213, 1784, 1897, 2621
PfoI TCCNGGA 1 cut(s) 1282
PinAI ACCGGT 1 cut(s) 796
PkrI GCNGC 8 cut(s) 244, 271, 931, 1084, 1731, 2377, 2506, 2612
PleI GAGTC 2 cut(s) 131, 2150
PmeI GTTTAAAC 1 cut(s) 388
PpsI GAGTC 2 cut(s) 131, 2150
Ppu21I YACGTR 3 cut(s) 606, 947, 2339
PpuMI RGGWCCY 1 cut(s) 2561
PscI ACATGT 1 cut(s) 442
PshAI GACNNNNGTC 1 cut(s) 2670
Psp5II RGGWCCY 1 cut(s) 2561
Psp6I CCWGG 6 cut(s) 1203, 1282, 1572, 1710, 1929, 2150
PspGI CCWGG 6 cut(s) 1203, 1282, 1572, 1710, 1929, 2150
PspN4I GGNNCC 5 cut(s) 27, 178, 544, 1434, 2405
PspPI GGNCC 4 cut(s) 684, 950, 1583, 2561
PspPPI RGGWCCY 1 cut(s) 2561
PstI CTGCAG 3 cut(s) 244, 934, 1185
PstNI CAGNNNCTG 2 cut(s) 239, 2720
PsuI RGATCY 4 cut(s) 25, 1577, 1735, 2716
PvuII CAGCTG 1 cut(s) 1732
RsaI GTAC 5 cut(s) 306, 593, 945, 1274, 2337
RsaNI GTAC 5 cut(s) 305, 592, 944, 1273, 2336
SatI GCNGC 8 cut(s) 243, 270, 930, 1083, 1730, 2376, 2505, 2611
Sau96I GGNCC 4 cut(s) 684, 950, 1583, 2561
ScaI AGTACT 1 cut(s) 1274
SchI GAGTC 2 cut(s) 131, 2150
ScrFI CCNGG 6 cut(s) 1205, 1284, 1574, 1712, 1931, 2152
SfcI CTRYAG 5 cut(s) 240, 930, 1181, 2205, 2499
SinI GGWCC 3 cut(s) 950, 1583, 2561
SmlI CTYRAG 2 cut(s) 360, 2054
SmoI CTYRAG 2 cut(s) 360, 2054
SphI GCATGC 1 cut(s) 2030
SsiI CCGC 5 cut(s) 659, 1251, 1482, 1514, 1638
SspMI CTAG 2 cut(s) 830, 2280
StyD4I CCNGG 6 cut(s) 1203, 1282, 1572, 1710, 1929, 2150
TaiI ACGT 4 cut(s) 286, 608, 949, 2341
TaqI TCGA 2 cut(s) 475, 1023
TaqII GACCGA 2 cut(s) 948, 2346
TatI WGTACW 3 cut(s) 304, 591, 1272
TfiI GAWTC 7 cut(s) 749, 809, 976, 1213, 1784, 1897, 2621
TscAI CASTG 5 cut(s) 244, 1059, 1666, 2376, 2518
TseFI GTSAC 5 cut(s) 311, 623, 1970, 2458, 2737
TseI GCWGC 8 cut(s) 242, 269, 929, 1082, 1729, 2375, 2504, 2610
Tsp45I GTSAC 5 cut(s) 311, 623, 1970, 2458, 2737
TspDTI ATGAA 7 cut(s) 243, 747, 1406, 1437, 1948, 2103, 2175
TspGWI ACGGA 3 cut(s) 54, 630, 660
TspRI CASTG 5 cut(s) 244, 1059, 1666, 2376, 2518
VpaK11BI GGWCC 3 cut(s) 950, 1583, 2561
XagI CCTNNNNNAGG 1 cut(s) 363
XapI RAATTY 9 cut(s) 525, 677, 741, 1019, 1242, 1633, 1777, 2048, 2182
XceI RCATGY 4 cut(s) 395, 446, 2030, 2317
XcmI CCANNNNNNNNNTGG 2 cut(s) 19, 203
XmnI GAANNNNTTC 4 cut(s) 585, 1212, 1437, 2673
XspI CTAG 2 cut(s) 830, 2280
ZrmI AGTACT 1 cut(s) 1274
Zsp2I ATGCAT 2 cut(s) 2028, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.