RchiOBHm_Chr4g0400761

Villin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001569
Physical Location & Seq
Forward (+)
18532507 .. 18534726
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37275

Sequence Viewer

Length: 255 bp
ATGGCTGTTTCCATGAGAGATTTGGATCCAGCTTTCCACGGAGCTGGACAAAAGGCTGGACTTGAAATATGGCGCATTGAAAACTTTCGTCCTGTTATTGTCCCACAATCCTCTCATGGAAAGTTTTTTATGGGGGACTCTTATGTGATCTTGAAAACAACAGCCTCAAAAAGTGGTGCCTTGCGACATGATATCCATTATTGGCTTGGTAAAGATACTTCTCAGGCACTGCAGCCATTAAGACAGTGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0006810 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010053 GO:0010054 GO:0010639 GO:0015629 GO:0016043 GO:0016049 GO:0016482 GO:0021700 GO:0022411 GO:0022622 GO:0030029 GO:0030036 GO:0030042 GO:0030154 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0031333 GO:0032271 GO:0032272 GO:0032432 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0033043 GO:0033993 GO:0040007 GO:0042221 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043242 GO:0043244 GO:0043254 GO:0043624 GO:0043933 GO:0044087 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048519 GO:0048523 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051014 GO:0051015 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051261 GO:0051493 GO:0051494 GO:0051592 GO:0051641 GO:0051649 GO:0051693 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071840 GO:0080147 GO:0090066 GO:0090558 GO:0090627 GO:0097305 GO:0097435 GO:0099402 GO:0099636 GO:0110053 GO:1901700 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04666 ko04810 ko05203 map04666 map04810 map05203
Pfam Domains
Protein Families
Gelsolin VHP

Protein Analysis

84

Amino Acids

9.51

Weight (kDa)

9.6

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 29 - 75 6.8e-06 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AclWI GGATC 2 cut(s) 20, 33
AgsI TTSAA 3 cut(s) 65, 80, 154
AluBI AGCT 2 cut(s) 32, 44
AluI AGCT 2 cut(s) 32, 44
AlwI GGATC 2 cut(s) 20, 33
AlwNI CAGNNNCTG 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 1 cut(s) 75
BamHI GGATCC 1 cut(s) 25
BanI GGYRCC 1 cut(s) 176
BarI GAAGNNNNNNTAC 2 cut(s) 202, 234
BbvI GCAGC 1 cut(s) 244
BfmI CTRYAG 1 cut(s) 230
BisI GCNGC 1 cut(s) 233
BlsI GCNGC 1 cut(s) 234
BmiI GGNNCC 2 cut(s) 27, 178
BsaBI GATNNNNATC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 37
BseJI GATNNNNATC 1 cut(s) 24
BseMII CTCAG 1 cut(s) 236
BseXI GCAGC 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 176
BslFI GGGAC 2 cut(s) 86, 149
BsmFI GGGAC 2 cut(s) 86, 149
Bsp143I GATC 2 cut(s) 25, 147
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 27, 178
BspMAI CTGCAG 1 cut(s) 234
BspPI GGATC 2 cut(s) 20, 33
BspT107I GGYRCC 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 2 cut(s) 25, 147
Bst4CI ACNGT 1 cut(s) 246
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 37
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 2 cut(s) 28, 150
BstMBI GATC 2 cut(s) 25, 147
BstSFI CTRYAG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 2 cut(s) 227, 251
CaiI CAGNNNCTG 1 cut(s) 229
CfoI GCGC 1 cut(s) 75
CviAII CATG 3 cut(s) 13, 116, 188
CviJI RGCY 7 cut(s) 5, 32, 44, 56, 164, 205, 235
CviKI_1 RGCY 7 cut(s) 5, 32, 44, 56, 164, 205, 235
DdeI CTNAG 1 cut(s) 222
DpnI GATC 2 cut(s) 27, 149
DpnII GATC 2 cut(s) 25, 147
Eco32I GATATC 1 cut(s) 193
EcoRV GATATC 1 cut(s) 193
FaeI CATG 3 cut(s) 16, 119, 191
FaiI YATR 6 cut(s) 14, 70, 117, 131, 144, 189
FaqI GGGAC 2 cut(s) 86, 149
FatI CATG 3 cut(s) 12, 115, 187
Fnu4HI GCNGC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 233
GlaI GCGC 1 cut(s) 74
GluI GCNGC 1 cut(s) 233
HhaI GCGC 1 cut(s) 75
Hin1II CATG 3 cut(s) 16, 119, 191
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 1 cut(s) 137
Hpy188III TCNNGA 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4V TGCA 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 3 cut(s) 16, 119, 191
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 2 cut(s) 25, 147
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 5 cut(s) 30, 42, 42, 105, 209
Lsp1109I GCAGC 1 cut(s) 244
MalI GATC 2 cut(s) 27, 149
MboI GATC 2 cut(s) 25, 147
MflI RGATCY 1 cut(s) 25
MluCI AATT 1 cut(s) 250
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 2 cut(s) 121, 175
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 1 cut(s) 239
NdeII GATC 2 cut(s) 25, 147
NlaIII CATG 3 cut(s) 16, 119, 191
NlaIV GGNNCC 2 cut(s) 27, 178
PdmI GAANNNNTTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 234
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 27, 178
PstI CTGCAG 1 cut(s) 234
PstNI CAGNNNCTG 1 cut(s) 229
PsuI RGATCY 1 cut(s) 25
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 1 cut(s) 233
Sau3AI GATC 2 cut(s) 25, 147
SchI GAGTC 1 cut(s) 131
SetI ASST 2 cut(s) 34, 46
SfcI CTRYAG 1 cut(s) 230
Sse9I AATT 1 cut(s) 250
TaaI ACNGT 1 cut(s) 246
TasI AATT 1 cut(s) 250
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TscAI CASTG 2 cut(s) 234, 251
TseI GCWGC 1 cut(s) 232
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 2 cut(s) 234, 251
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 203, 243
XmnI GAANNNNTTC 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.