AT5G57370

U4 U6.U5 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010002
Physical Location & Seq
Forward (+)
23244610 .. 23246420
1811 bp
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UTR
Exon/CDS
Intron
AT5G57370.1

Sequence Viewer

Length: 660 bp
ATGTCAGAGCGCGACCGCCGCGAACGAGAGAGGGATAAGGACCGTGAACCAGACCGTGATAGACGCCGAGGTAGAGACGACCGTGACCGTGACCGTGATCGAGACCGGGAAAGAGATAGAGAGCGCGACCGTGACCGAGGGCTTAGAAACAAAAAATCCCGATCTCGTACTCCAGACCACCACGCTCGTGCTCGCCACGTGCGCTCTCCGGAGAGGTATCGCTCTCGTTCCCGCTCGATTGACCGTGACCGGGACCGGGACCGTCAACGACACCACAGGCGCAGGTCTCCTTCTCCAGATGCTCCGTCGCGAAAACGGCCTCGCCAGGGTTCAGTAGACGATGAGAAAGAAAGGAATCGCAAAAGAGTAGTGACTGATTCCGTTGACGAGAATGCGTCTACTATTACTAAGAAGAAGGATAAACAACCAAGTGATGCTGCTGATAATGGCGGAGGCGAAGATGAAGAAGGAATGGATGTGAATGAGATTGAGATGATGAAGATGTTAGGGATTCCTACTGGATTTGATTCCACAAAAGGCAAACCAGTTGCTGGTGCTGATGTTAGTGGTATTAGAGCTGTAACTAAGCGTCAGCCAAGACAGTACATGAACCGTCGCGGTGGATTTAACCGTCCTCTACCGGCCGAGCGTAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

219

Amino Acids

26.04

Weight (kDa)

10.97

Isoelectric Point (pI)

72.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 162 - 215 8e-23 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AccB7I CCANNNNNTGG 1 cut(s) 551
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 2 cut(s) 336, 398
AccII CGCG 5 cut(s) 12, 21, 126, 310, 618
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 5 cut(s) 16, 19, 232, 450, 618
AcoI YGGCCR 1 cut(s) 642
AcvI CACGTG 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 64
AfaI GTAC 2 cut(s) 169, 605
AfiI CCNNNNNNNGG 4 cut(s) 250, 256, 326, 551
AjnI CCWGG 1 cut(s) 324
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 1 cut(s) 578
AluI AGCT 1 cut(s) 578
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 3 cut(s) 69, 96, 291
AlwNI CAGNNNCTG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 2 cut(s) 317, 642
ApeKI GCWGC 1 cut(s) 437
AspLEI GCGC 4 cut(s) 12, 126, 204, 282
AspS9I GGNCC 3 cut(s) 40, 253, 259
AsuC2I CCSGG 3 cut(s) 107, 251, 257
AvaII GGWCC 3 cut(s) 40, 253, 259
BauI CACGAG 1 cut(s) 186
BbrPI CACGTG 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 1 cut(s) 424
BceAI ACGGC 1 cut(s) 332
BciT130I CCWGG 1 cut(s) 326
BcnI CCSGG 3 cut(s) 107, 251, 257
BcoDI GTCTC 3 cut(s) 69, 96, 291
BfaI CTAG 1 cut(s) 658
BfuAI ACCTGC 1 cut(s) 273
BisI GCNGC 2 cut(s) 19, 438
BlsI GCNGC 2 cut(s) 20, 439
Bme1390I CCNGG 4 cut(s) 107, 251, 257, 326
Bme18I GGWCC 3 cut(s) 40, 253, 259
BmgT120I GGNCC 3 cut(s) 40, 253, 259
BmiI GGNNCC 2 cut(s) 254, 260
BmrFI CCNGG 4 cut(s) 107, 251, 257, 326
BmsI GCATC 2 cut(s) 289, 424
BpmI CTGGAG 2 cut(s) 156, 279
BpuMI CCSGG 3 cut(s) 107, 251, 257
BsaAI YACGTR 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 64
BsaI GGTCTC 2 cut(s) 96, 291
BsaJI CCNNGG 3 cut(s) 67, 136, 325
BsaWI WCCGGW 1 cut(s) 208
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 256, 326, 551
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 2 cut(s) 523, 545
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 3 cut(s) 67, 136, 325
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 4 cut(s) 250, 256, 326, 551
BseNI ACTGG 2 cut(s) 523, 545
BseX3I CGGCCG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 424
Bsh1236I CGCG 5 cut(s) 12, 21, 126, 310, 618
Bsh1285I CGRYCG 4 cut(s) 16, 82, 130, 645
BshFI GGCC 2 cut(s) 319, 644
BsiEI CGRYCG 4 cut(s) 16, 82, 130, 645
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 5 cut(s) 106, 209, 250, 256, 641
BslFI GGGAC 2 cut(s) 266, 272
BslI CCNNNNNNNGG 4 cut(s) 250, 256, 326, 551
BsmAI GTCTC 3 cut(s) 69, 96, 291
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 266, 272
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 319, 644
Bso31I GGTCTC 2 cut(s) 96, 291
Bsp1286I GDGCHC 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 2 cut(s) 97, 161
Bsp68I TCGCGA 1 cut(s) 310
BspACI CCGC 5 cut(s) 16, 19, 232, 450, 618
BspANI GGCC 2 cut(s) 319, 644
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 5 cut(s) 12, 21, 126, 310, 618
BspLI GGNNCC 2 cut(s) 254, 260
BspMI ACCTGC 1 cut(s) 273
BspTNI GGTCTC 2 cut(s) 96, 291
BsrBI CCGCTC 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 640
BsrI ACTGG 2 cut(s) 523, 545
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 3 cut(s) 67, 136, 325
BssMI GATC 2 cut(s) 97, 161
BssNI GRCGYC 1 cut(s) 64
BssSI CACGAG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 326
BstACI GRCGYC 1 cut(s) 64
BstBAI YACGTR 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 143, 408, 585
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 5 cut(s) 12, 21, 126, 310, 618
BstHHI GCGC 4 cut(s) 12, 126, 204, 282
BstKTI GATC 2 cut(s) 100, 164
BstMAI GTCTC 3 cut(s) 69, 96, 291
BstMBI GATC 2 cut(s) 97, 161
BstMCI CGRYCG 4 cut(s) 16, 82, 130, 645
BstMWI GCNNNNNNNGC 3 cut(s) 18, 201, 316
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 4 cut(s) 105, 249, 255, 324
BstUI CGCG 5 cut(s) 12, 21, 126, 310, 618
BstV1I GCAGC 1 cut(s) 424
BstZI CGGCCG 1 cut(s) 642
BsuRI GGCC 2 cut(s) 319, 644
BtsCI GGATG 1 cut(s) 481
BtuMI TCGCGA 1 cut(s) 310
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 193
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 4 cut(s) 12, 126, 204, 282
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 3 cut(s) 40, 253, 259
CseI GACGC 3 cut(s) 72, 384, 578
Csp6I GTAC 2 cut(s) 168, 604
CviAII CATG 1 cut(s) 607
CviJI RGCY 5 cut(s) 142, 319, 578, 595, 644
CviKI_1 RGCY 5 cut(s) 142, 319, 578, 595, 644
CviQI GTAC 2 cut(s) 168, 604
DdeI CTNAG 3 cut(s) 143, 408, 585
DpnI GATC 2 cut(s) 99, 163
DpnII GATC 2 cut(s) 97, 161
EaeI YGGCCR 1 cut(s) 642
EagI CGGCCG 1 cut(s) 642
EciI GGCGGA 1 cut(s) 465
EclXI CGGCCG 1 cut(s) 642
Eco31I GGTCTC 2 cut(s) 96, 291
Eco47I GGWCC 3 cut(s) 40, 253, 259
Eco52I CGGCCG 1 cut(s) 642
Eco72I CACGTG 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 1 cut(s) 610
FaiI YATR 1 cut(s) 608
FaqI GGGAC 2 cut(s) 266, 272
FatI CATG 1 cut(s) 606
FauI CCCGC 1 cut(s) 239
FblI GTMKAC 2 cut(s) 336, 398
Fnu4HI GCNGC 2 cut(s) 19, 438
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 2 cut(s) 19, 438
FspBI CTAG 1 cut(s) 658
GlaI GCGC 4 cut(s) 11, 125, 203, 281
GluI GCNGC 2 cut(s) 19, 438
GsuI CTGGAG 2 cut(s) 156, 279
HaeIII GGCC 2 cut(s) 319, 644
HapII CCGG 5 cut(s) 106, 209, 250, 256, 641
HgaI GACGC 3 cut(s) 72, 384, 578
HhaI GCGC 4 cut(s) 12, 126, 204, 282
Hin1I GRCGYC 1 cut(s) 64
Hin1II CATG 1 cut(s) 610
Hin6I GCGC 4 cut(s) 10, 124, 202, 280
HinP1I GCGC 4 cut(s) 10, 124, 202, 280
HincII GTYRAC 2 cut(s) 266, 385
HindII GTYRAC 2 cut(s) 266, 385
HinfI GANTC 4 cut(s) 355, 377, 511, 527
HpaII CCGG 5 cut(s) 106, 209, 250, 256, 641
Hpy166II GTNNAC 5 cut(s) 47, 266, 337, 385, 399
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 6 cut(s) 101, 159, 173, 209, 296, 309
Hpy8I GTNNAC 5 cut(s) 47, 266, 337, 385, 399
Hpy99I CGWCG 2 cut(s) 310, 618
HpyAV CCTTC 3 cut(s) 300, 409, 461
HpyCH4IV ACGT 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 201, 316
HpyF3I CTNAG 3 cut(s) 143, 408, 585
HpySE526I ACGT 1 cut(s) 198
Hsp92I GRCGYC 1 cut(s) 64
Hsp92II CATG 1 cut(s) 610
HspAI GCGC 4 cut(s) 10, 124, 202, 280
Kpn2I TCCGGA 1 cut(s) 208
Kzo9I GATC 2 cut(s) 97, 161
LmnI GCTCC 1 cut(s) 307
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 2 cut(s) 289, 424
MaeI CTAG 1 cut(s) 658
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 6 cut(s) 83, 89, 131, 245, 370, 580
MalI GATC 2 cut(s) 99, 163
MbiI CCGCTC 1 cut(s) 234
MboI GATC 2 cut(s) 97, 161
MboII GAAGA 4 cut(s) 424, 470, 476, 511
MhlI GDGCHC 1 cut(s) 193
MnlI CCTC 7 cut(s) 24, 62, 131, 207, 330, 446, 645
MroI TCCGGA 1 cut(s) 208
MseI TTAA 1 cut(s) 627
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 5 cut(s) 106, 209, 250, 256, 641
MspR9I CCNGG 4 cut(s) 107, 251, 257, 326
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 5 cut(s) 12, 21, 126, 310, 618
MwoI GCNNNNNNNGC 3 cut(s) 18, 201, 316
NciI CCSGG 3 cut(s) 107, 251, 257
NdeII GATC 2 cut(s) 97, 161
NlaIII CATG 1 cut(s) 610
NlaIV GGNNCC 2 cut(s) 254, 260
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 5 cut(s) 83, 89, 131, 245, 370
NruI TCGCGA 1 cut(s) 310
OliI CACNNNNGTG 1 cut(s) 186
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 4 cut(s) 355, 377, 511, 527
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 2 cut(s) 20, 439
PmaCI CACGTG 1 cut(s) 199
PmlI CACGTG 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 324
PspCI CACGTG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 2 cut(s) 254, 260
PspPI GGNCC 3 cut(s) 40, 253, 259
PstNI CAGNNNCTG 1 cut(s) 551
RruI TCGCGA 1 cut(s) 310
RsaI GTAC 2 cut(s) 169, 605
RsaNI GTAC 2 cut(s) 168, 604
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 2 cut(s) 19, 438
Sau3AI GATC 2 cut(s) 97, 161
Sau96I GGNCC 3 cut(s) 40, 253, 259
ScrFI CCNGG 4 cut(s) 107, 251, 257, 326
SduI GDGCHC 1 cut(s) 193
SetI ASST 5 cut(s) 73, 201, 218, 287, 580
SfaNI GCATC 2 cut(s) 289, 424
SinI GGWCC 3 cut(s) 40, 253, 259
SmiMI CAYNNNNRTG 1 cut(s) 186
SsiI CCGC 5 cut(s) 16, 19, 232, 450, 618
SspMI CTAG 1 cut(s) 658
StyD4I CCNGG 4 cut(s) 105, 249, 255, 324
TaiI ACGT 1 cut(s) 201
TaqI TCGA 2 cut(s) 100, 236
TaqII GACCGA 1 cut(s) 150
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 4 cut(s) 355, 377, 511, 527
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TseFI GTSAC 5 cut(s) 83, 89, 131, 245, 370
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 5 cut(s) 83, 89, 131, 245, 370
TspDTI ATGAA 3 cut(s) 477, 512, 623
TspGWI ACGGA 2 cut(s) 294, 370
Van91I CCANNNNNTGG 1 cut(s) 551
VpaK11BI GGWCC 3 cut(s) 40, 253, 259
XmiI GTMKAC 2 cut(s) 336, 398
XspI CTAG 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.