Rh4DG100100

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010002
Physical Location & Seq
Forward (+)
15240186 .. 15240791
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG100100.1

Sequence Viewer

Length: 606 bp
ATGGGAGACCGAGAAAGAGAGCGTGACAGAGAACGAGACCGGGAGAGAATCCGAGACCGGGACCGAGACAGAGACAGACGCCGTGACAGAGACGACCGTGATCGTGACCGTGACCGCGACCGCGTCCGGAGCAAGAGGTCACGTACGCCGGAGCGCAGCAGAGCACGCCACACGCGCTCCCGCACGCGCTCGCCGGACTTGTACCACTCACGCTCCCTCTCCCGTTCCCGCACTCCAGAAGACCGCTCCCACCGCCGCCGCCACCACCACCGCAGCCCCTCCCCGGAGTCGCGGAAGCGTGCGCGGCGCGAGCCGACGGTGGAGGAGGAGAAGGACCGGCAGAAGGTGGTGTCGGAATTCGTGGAGGGGATTGCGAAGGAGCAGCAGAAGCAGAGCGGCGGTGGTGGAGAGGGCGGTGCGGACGAGGACGAGGTTGAGATGATGAAGAAGTTGGGGATTCCGGTAGGGTTTGATTCCACGAAAGGGAAGCCGGTGCCGGGGGCCGATGTGAGTGGTGTTAGGGCCGTCACGAAGCGCCAGCCTAGACAGTACATGAACCGCCGCGGCGGATTTAACCGGCCGTTGCCTGCAGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

201

Amino Acids

23.92

Weight (kDa)

11.26

Isoelectric Point (pI)

87.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 142 - 197 2.6e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 493
AccBSI CCGCTC 2 cut(s) 246, 396
AccII CGCG 8 cut(s) 117, 123, 175, 187, 292, 304, 309, 564
AccIII TCCGGA 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 578
AcsI RAATTY 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 3 cut(s) 145, 203, 551
AfiI CCNNNNNNNGG 5 cut(s) 58, 283, 343, 483, 497
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 5 cut(s) 30, 48, 60, 66, 84
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 3 cut(s) 501, 522, 578
ApeKI GCWGC 3 cut(s) 156, 273, 382
ApoI RAATTY 1 cut(s) 356
AspLEI GCGC 7 cut(s) 156, 177, 189, 304, 309, 537, 597
AspS9I GGNCC 4 cut(s) 61, 334, 501, 522
AsuC2I CCSGG 4 cut(s) 41, 59, 284, 498
AvaII GGWCC 2 cut(s) 61, 334
BanI GGYRCC 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 3 cut(s) 168, 285, 394
BceAI ACGGC 3 cut(s) 66, 509, 565
BcnI CCSGG 4 cut(s) 41, 59, 284, 498
BcoDI GTCTC 5 cut(s) 30, 48, 60, 66, 84
BfaI CTAG 2 cut(s) 543, 604
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 538
BisI GCNGC 9 cut(s) 157, 256, 259, 274, 305, 383, 397, 562, 565
BlsI GCNGC 9 cut(s) 158, 257, 260, 275, 306, 384, 398, 563, 566
Bme1390I CCNGG 4 cut(s) 41, 59, 284, 498
Bme18I GGWCC 2 cut(s) 61, 334
BmgT120I GGNCC 4 cut(s) 61, 334, 501, 522
BmiI GGNNCC 3 cut(s) 62, 495, 502
BmrFI CCNGG 4 cut(s) 41, 59, 284, 498
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 219
BpuMI CCSGG 4 cut(s) 41, 59, 284, 498
BsaAI YACGTR 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 30, 48
BsaJI CCNNGG 3 cut(s) 282, 497, 562
BsaWI WCCGGW 2 cut(s) 126, 460
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 197, 227
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 283, 343, 483, 497
Bse118I RCCGGY 3 cut(s) 336, 490, 576
BseAI TCCGGA 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 282, 497, 562
BseLI CCNNNNNNNGG 5 cut(s) 58, 283, 343, 483, 497
BseRI GAGGAG 2 cut(s) 338, 341
BseX3I CGGCCG 1 cut(s) 578
BseXI GCAGC 3 cut(s) 168, 285, 394
Bsh1236I CGCG 8 cut(s) 117, 123, 175, 187, 292, 304, 309, 564
Bsh1285I CGRYCG 3 cut(s) 97, 121, 581
BshFI GGCC 3 cut(s) 503, 524, 580
BshNI GGYRCC 1 cut(s) 493
BsiEI CGRYCG 3 cut(s) 97, 121, 581
BsiHKAI GWGCWC 1 cut(s) 166
BsiWI CGTACG 1 cut(s) 143
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 5 cut(s) 58, 283, 343, 483, 497
BsmAI GTCTC 5 cut(s) 30, 48, 60, 66, 84
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 3 cut(s) 503, 524, 580
Bso31I GGTCTC 2 cut(s) 30, 48
Bsp1286I GDGCHC 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 1 cut(s) 100
BspANI GGCC 3 cut(s) 503, 524, 580
BspEI TCCGGA 1 cut(s) 126
BspFNI CGCG 8 cut(s) 117, 123, 175, 187, 292, 304, 309, 564
BspLI GGNNCC 3 cut(s) 62, 495, 502
BspMAI CTGCAG 1 cut(s) 592
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 2 cut(s) 30, 48
BsrBI CCGCTC 2 cut(s) 246, 396
BsrFI RCCGGY 3 cut(s) 336, 490, 576
BssAI RCCGGY 3 cut(s) 336, 490, 576
BssECI CCNNGG 3 cut(s) 282, 497, 562
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 1 cut(s) 79
Bst4CI ACNGT 4 cut(s) 98, 110, 319, 549
BstACI GRCGYC 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 143
BstC8I GCNNGC 7 cut(s) 166, 185, 191, 300, 311, 539, 588
BstDSI CCRYGG 1 cut(s) 562
BstFNI CGCG 8 cut(s) 117, 123, 175, 187, 292, 304, 309, 564
BstH2I RGCGCY 1 cut(s) 538
BstHHI GCGC 7 cut(s) 156, 177, 189, 304, 309, 537, 597
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 5 cut(s) 30, 48, 60, 66, 84
BstMBI GATC 1 cut(s) 100
BstMCI CGRYCG 3 cut(s) 97, 121, 581
BstMWI GCNNNNNNNGC 8 cut(s) 129, 165, 174, 252, 304, 310, 388, 592
BstSCI CCNGG 4 cut(s) 39, 57, 282, 496
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 8 cut(s) 117, 123, 175, 187, 292, 304, 309, 564
BstV1I GCAGC 3 cut(s) 168, 285, 394
BstV2I GAAGAC 1 cut(s) 246
BstZI CGGCCG 1 cut(s) 578
BsuRI GGCC 3 cut(s) 503, 524, 580
BtgI CCRYGG 1 cut(s) 562
Cac8I GCNNGC 7 cut(s) 166, 185, 191, 300, 311, 539, 588
CfoI GCGC 7 cut(s) 156, 177, 189, 304, 309, 537, 597
Cfr10I RCCGGY 3 cut(s) 336, 490, 576
Cfr13I GGNCC 4 cut(s) 61, 334, 501, 522
Cfr42I CCGCGG 1 cut(s) 565
CseI GACGC 2 cut(s) 87, 112
Csp6I GTAC 3 cut(s) 144, 202, 550
CviAII CATG 1 cut(s) 553
CviJI RGCY 7 cut(s) 276, 313, 490, 503, 524, 541, 580
CviKI_1 RGCY 7 cut(s) 276, 313, 490, 503, 524, 541, 580
CviQI GTAC 3 cut(s) 144, 202, 550
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 578
EagI CGGCCG 1 cut(s) 578
EciI GGCGGA 1 cut(s) 582
EclXI CGGCCG 1 cut(s) 578
Eco31I GGTCTC 2 cut(s) 30, 48
Eco47I GGWCC 2 cut(s) 61, 334
Eco52I CGGCCG 1 cut(s) 578
EcoRI GAATTC 1 cut(s) 356
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 1 cut(s) 556
FaiI YATR 1 cut(s) 554
FaqI GGGAC 1 cut(s) 74
FatI CATG 1 cut(s) 552
FauI CCCGC 2 cut(s) 188, 236
Fnu4HI GCNGC 9 cut(s) 157, 256, 259, 274, 305, 383, 397, 562, 565
Fsp4HI GCNGC 9 cut(s) 157, 256, 259, 274, 305, 383, 397, 562, 565
FspBI CTAG 2 cut(s) 543, 604
GlaI GCGC 7 cut(s) 155, 176, 188, 303, 308, 536, 596
GluI GCNGC 9 cut(s) 157, 256, 259, 274, 305, 383, 397, 562, 565
GsuI CTGGAG 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 538
HaeIII GGCC 3 cut(s) 503, 524, 580
HgaI GACGC 2 cut(s) 87, 112
HhaI GCGC 7 cut(s) 156, 177, 189, 304, 309, 537, 597
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 1 cut(s) 556
Hin6I GCGC 7 cut(s) 154, 175, 187, 302, 307, 535, 595
HinP1I GCGC 7 cut(s) 154, 175, 187, 302, 307, 535, 595
HinfI GANTC 4 cut(s) 48, 287, 457, 473
Hpy188I TCNGA 2 cut(s) 53, 355
Hpy188III TCNNGA 4 cut(s) 104, 127, 236, 529
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 3 cut(s) 325, 337, 370
HpyCH4III ACNGT 4 cut(s) 98, 110, 319, 549
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 8 cut(s) 129, 165, 174, 252, 304, 310, 388, 592
HpySE526I ACGT 1 cut(s) 142
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 1 cut(s) 556
HspAI GCGC 7 cut(s) 154, 175, 187, 302, 307, 535, 595
Kpn2I TCCGGA 1 cut(s) 126
KspI CCGCGG 1 cut(s) 565
Kzo9I GATC 1 cut(s) 100
LmnI GCTCC 6 cut(s) 129, 151, 182, 218, 251, 379
Lsp1109I GCAGC 3 cut(s) 168, 285, 394
MaeI CTAG 2 cut(s) 543, 604
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 6 cut(s) 23, 83, 104, 110, 138, 526
MalI GATC 1 cut(s) 102
MbiI CCGCTC 2 cut(s) 246, 396
MboI GATC 1 cut(s) 100
MboII GAAGA 2 cut(s) 251, 457
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 1 cut(s) 356
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 9 cut(s) 129, 227, 289, 316, 319, 358, 403, 418, 424
MroI TCCGGA 1 cut(s) 126
MseI TTAA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 564
MspR9I CCNGG 4 cut(s) 41, 59, 284, 498
MteI GCGCNGCGC 1 cut(s) 305
MvnI CGCG 8 cut(s) 117, 123, 175, 187, 292, 304, 309, 564
MwoI GCNNNNNNNGC 8 cut(s) 129, 165, 174, 252, 304, 310, 388, 592
NciI CCSGG 4 cut(s) 41, 59, 284, 498
NdeII GATC 1 cut(s) 100
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 3 cut(s) 62, 495, 502
NmuCI GTSAC 6 cut(s) 23, 83, 104, 110, 138, 526
PfeI GAWTC 3 cut(s) 48, 457, 473
Pfl23II CGTACG 1 cut(s) 143
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 9 cut(s) 158, 257, 260, 275, 306, 384, 398, 563, 566
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 143
PspLI CGTACG 1 cut(s) 143
PspN4I GGNNCC 3 cut(s) 62, 495, 502
PspPI GGNCC 4 cut(s) 61, 334, 501, 522
PstI CTGCAG 1 cut(s) 592
PsyI GACNNNGTC 1 cut(s) 122
RsaI GTAC 3 cut(s) 145, 203, 551
RsaNI GTAC 3 cut(s) 144, 202, 550
SacII CCGCGG 1 cut(s) 565
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 9 cut(s) 157, 256, 259, 274, 305, 383, 397, 562, 565
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 4 cut(s) 61, 334, 501, 522
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 4 cut(s) 41, 59, 284, 498
SduI GDGCHC 1 cut(s) 166
SetI ASST 4 cut(s) 140, 145, 348, 435
SfcI CTRYAG 1 cut(s) 588
Sfr303I CCGCGG 1 cut(s) 565
SgrBI CCGCGG 1 cut(s) 565
SinI GGWCC 2 cut(s) 61, 334
Sse9I AATT 1 cut(s) 356
SspMI CTAG 2 cut(s) 543, 604
StyD4I CCNGG 4 cut(s) 39, 57, 282, 496
TaaI ACNGT 4 cut(s) 98, 110, 319, 549
TaiI ACGT 1 cut(s) 145
TaqII GACCGA 2 cut(s) 24, 78
TasI AATT 1 cut(s) 356
TatI WGTACW 1 cut(s) 549
TauI GCSGC 6 cut(s) 258, 261, 307, 399, 564, 567
TfiI GAWTC 3 cut(s) 48, 457, 473
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 6 cut(s) 23, 83, 104, 110, 138, 526
TseI GCWGC 3 cut(s) 156, 273, 382
Tsp45I GTSAC 6 cut(s) 23, 83, 104, 110, 138, 526
TspDTI ATGAA 2 cut(s) 458, 569
Tth111I GACNNNGTC 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 61, 334
XapI RAATTY 1 cut(s) 356
XspI CTAG 2 cut(s) 543, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.