Rh4AG108400

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010002
Physical Location & Seq
Forward (+)
23599023 .. 23599640
618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG108400.1

Sequence Viewer

Length: 618 bp
ATGGGAGACCGAGAAAGAGAGCGAGACAGAGAACGAGACCGGGAGAGAATCCGAGACCGGGATCGAGACAGAGACAGACGCCGTGACAGAGACGACCGTGATCGTGACCGTGACCGTGACCGTGACCGCGACCGTGTCCGGAGCAAGAGGTCACGTACGCCGGAGCGCAGCAGAGCACGCCACACGCGCTCCCGCACGCGCTCGCCGGACTTGTACCACTCACGCTCCCTCTCCCGTTCCCGCACTCCAGAAGACCGCTCCCACCGCCGCCGCCACCACCACCGCAGCCCCTCCCCGGAGTCGCGGAAGCGTGCGCGGCGCGAGGCGACGGTGGAGGAGGAGAAGGACCGGCAGAAGGTGGTGTCGGAATTCGTGGAGGGGATTGCGAAGGAGCAGCAGAAGCAGAGCAGCGGTGGTGGAGAGGGCGGTGCGGACGAGGACGAGGTTGAGATGATGAAGAAGTTGGGGATTCCGGTAGGGTTTGATTCCACGAAGGGGAAGCCGGTGCAGGGGGCCGATGTGAGTGGTGTTAGGGCCGTCACGAAGCGCCAGCCTAGACAGTACATGAACCGCCGCGGCGGATTTAACCGGCCATTGCCTGCAGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

205

Amino Acids

24.5

Weight (kDa)

11.24

Isoelectric Point (pI)

83.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 146 - 201 1.7e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AccII CGCG 7 cut(s) 129, 187, 199, 304, 316, 321, 576
AccIII TCCGGA 1 cut(s) 138
AclWI GGATC 1 cut(s) 69
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 3 cut(s) 157, 215, 563
AfiI CCNNNNNNNGG 5 cut(s) 58, 295, 355, 495, 509
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
AlwI GGATC 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 3 cut(s) 513, 534, 590
ApeKI GCWGC 4 cut(s) 168, 285, 394, 408
ApoI RAATTY 1 cut(s) 368
AspLEI GCGC 7 cut(s) 168, 189, 201, 316, 321, 549, 609
AspS9I GGNCC 3 cut(s) 346, 513, 534
AsuC2I CCSGG 3 cut(s) 41, 59, 296
AvaII GGWCC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 4 cut(s) 180, 297, 406, 420
BceAI ACGGC 2 cut(s) 66, 521
BcnI CCSGG 3 cut(s) 41, 59, 296
BcoDI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BfaI CTAG 2 cut(s) 555, 616
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 550
BisI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 574, 577
BlsI GCNGC 9 cut(s) 170, 269, 272, 287, 318, 396, 410, 575, 578
Bme1390I CCNGG 3 cut(s) 41, 59, 296
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 3 cut(s) 346, 513, 534
BmiI GGNNCC 1 cut(s) 514
BmrFI CCNGG 3 cut(s) 41, 59, 296
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 231
BpuMI CCSGG 3 cut(s) 41, 59, 296
BsaAI YACGTR 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 30, 48
BsaJI CCNNGG 2 cut(s) 294, 574
BsaWI WCCGGW 2 cut(s) 138, 472
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 209, 239
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 295, 355, 495, 509
Bse118I RCCGGY 3 cut(s) 348, 502, 588
Bse3DI GCAATG 1 cut(s) 593
BseAI TCCGGA 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 294, 574
BseLI CCNNNNNNNGG 5 cut(s) 58, 295, 355, 495, 509
BseMI GCAATG 1 cut(s) 593
BseRI GAGGAG 2 cut(s) 350, 353
BseXI GCAGC 4 cut(s) 180, 297, 406, 420
BsgI GTGCAG 1 cut(s) 527
Bsh1236I CGCG 7 cut(s) 129, 187, 199, 304, 316, 321, 576
Bsh1285I CGRYCG 2 cut(s) 97, 133
BshFI GGCC 3 cut(s) 515, 536, 592
BsiEI CGRYCG 2 cut(s) 97, 133
BsiHKAI GWGCWC 1 cut(s) 178
BsiWI CGTACG 1 cut(s) 155
BslI CCNNNNNNNGG 5 cut(s) 58, 295, 355, 495, 509
BsmAI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BsmBI CGTCTC 1 cut(s) 84
BsnI GGCC 3 cut(s) 515, 536, 592
Bso31I GGTCTC 2 cut(s) 30, 48
Bsp1286I GDGCHC 1 cut(s) 178
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 2 cut(s) 61, 100
BspANI GGCC 3 cut(s) 515, 536, 592
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 7 cut(s) 129, 187, 199, 304, 316, 321, 576
BspLI GGNNCC 1 cut(s) 514
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 1 cut(s) 69
BspTNI GGTCTC 2 cut(s) 30, 48
BsrBI CCGCTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 593
BsrFI RCCGGY 3 cut(s) 348, 502, 588
BssAI RCCGGY 3 cut(s) 348, 502, 588
BssECI CCNNGG 2 cut(s) 294, 574
BssMI GATC 2 cut(s) 61, 100
BssNI GRCGYC 1 cut(s) 79
Bst4CI ACNGT 7 cut(s) 98, 110, 116, 122, 134, 331, 561
BstACI GRCGYC 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 6 cut(s) 178, 197, 203, 312, 551, 600
BstDSI CCRYGG 1 cut(s) 574
BstFNI CGCG 7 cut(s) 129, 187, 199, 304, 316, 321, 576
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 7 cut(s) 168, 189, 201, 316, 321, 549, 609
BstKTI GATC 2 cut(s) 64, 103
BstMAI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BstMBI GATC 2 cut(s) 61, 100
BstMCI CGRYCG 2 cut(s) 97, 133
BstMWI GCNNNNNNNGC 6 cut(s) 177, 186, 264, 316, 400, 604
BstSCI CCNGG 3 cut(s) 39, 57, 294
BstSFI CTRYAG 1 cut(s) 600
BstUI CGCG 7 cut(s) 129, 187, 199, 304, 316, 321, 576
BstV1I GCAGC 4 cut(s) 180, 297, 406, 420
BstV2I GAAGAC 1 cut(s) 258
BsuRI GGCC 3 cut(s) 515, 536, 592
BtgI CCRYGG 1 cut(s) 574
Cac8I GCNNGC 6 cut(s) 178, 197, 203, 312, 551, 600
CfoI GCGC 7 cut(s) 168, 189, 201, 316, 321, 549, 609
Cfr10I RCCGGY 3 cut(s) 348, 502, 588
Cfr13I GGNCC 3 cut(s) 346, 513, 534
Cfr42I CCGCGG 1 cut(s) 577
CseI GACGC 1 cut(s) 87
Csp6I GTAC 3 cut(s) 156, 214, 562
CviAII CATG 1 cut(s) 565
CviJI RGCY 6 cut(s) 288, 502, 515, 536, 553, 592
CviKI_1 RGCY 6 cut(s) 288, 502, 515, 536, 553, 592
CviQI GTAC 3 cut(s) 156, 214, 562
DpnI GATC 2 cut(s) 63, 102
DpnII GATC 2 cut(s) 61, 100
EaeI YGGCCR 1 cut(s) 590
EciI GGCGGA 1 cut(s) 594
Eco31I GGTCTC 2 cut(s) 30, 48
Eco47I GGWCC 1 cut(s) 346
EcoRI GAATTC 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 1 cut(s) 568
FaiI YATR 1 cut(s) 566
FatI CATG 1 cut(s) 564
FauI CCCGC 2 cut(s) 200, 248
Fnu4HI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 574, 577
Fsp4HI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 574, 577
FspBI CTAG 2 cut(s) 555, 616
GlaI GCGC 7 cut(s) 167, 188, 200, 315, 320, 548, 608
GluI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 574, 577
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 3 cut(s) 515, 536, 592
HgaI GACGC 1 cut(s) 87
HhaI GCGC 7 cut(s) 168, 189, 201, 316, 321, 549, 609
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 1 cut(s) 568
Hin6I GCGC 7 cut(s) 166, 187, 199, 314, 319, 547, 607
HinP1I GCGC 7 cut(s) 166, 187, 199, 314, 319, 547, 607
HinfI GANTC 4 cut(s) 48, 299, 469, 485
Hpy188I TCNGA 2 cut(s) 53, 367
Hpy188III TCNNGA 5 cut(s) 65, 104, 139, 248, 541
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 4 cut(s) 337, 349, 382, 487
HpyCH4III ACNGT 7 cut(s) 98, 110, 116, 122, 134, 331, 561
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 508, 602
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 186, 264, 316, 400, 604
HpySE526I ACGT 1 cut(s) 154
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 1 cut(s) 568
HspAI GCGC 7 cut(s) 166, 187, 199, 314, 319, 547, 607
Kpn2I TCCGGA 1 cut(s) 138
KspI CCGCGG 1 cut(s) 577
Kzo9I GATC 2 cut(s) 61, 100
LmnI GCTCC 6 cut(s) 141, 163, 194, 230, 263, 391
Lsp1109I GCAGC 4 cut(s) 180, 297, 406, 420
MaeI CTAG 2 cut(s) 555, 616
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 7 cut(s) 83, 104, 110, 116, 122, 150, 538
MalI GATC 2 cut(s) 63, 102
MbiI CCGCTC 1 cut(s) 258
MboI GATC 2 cut(s) 61, 100
MboII GAAGA 2 cut(s) 263, 469
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 1 cut(s) 368
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 345
MroI TCCGGA 1 cut(s) 138
MseI TTAA 1 cut(s) 585
MspA1I CMGCKG 2 cut(s) 411, 576
MspR9I CCNGG 3 cut(s) 41, 59, 296
MteI GCGCNGCGC 1 cut(s) 317
MvnI CGCG 7 cut(s) 129, 187, 199, 304, 316, 321, 576
MwoI GCNNNNNNNGC 6 cut(s) 177, 186, 264, 316, 400, 604
NciI CCSGG 3 cut(s) 41, 59, 296
NdeII GATC 2 cut(s) 61, 100
NlaIII CATG 1 cut(s) 568
NlaIV GGNNCC 1 cut(s) 514
NmuCI GTSAC 7 cut(s) 83, 104, 110, 116, 122, 150, 538
PfeI GAWTC 3 cut(s) 48, 469, 485
Pfl23II CGTACG 1 cut(s) 155
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 9 cut(s) 170, 269, 272, 287, 318, 396, 410, 575, 578
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Ppu21I YACGTR 1 cut(s) 155
PspLI CGTACG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 514
PspPI GGNCC 3 cut(s) 346, 513, 534
PstI CTGCAG 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 3 cut(s) 157, 215, 563
RsaNI GTAC 3 cut(s) 156, 214, 562
SacII CCGCGG 1 cut(s) 577
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 574, 577
Sau3AI GATC 2 cut(s) 61, 100
Sau96I GGNCC 3 cut(s) 346, 513, 534
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 41, 59, 296
SduI GDGCHC 1 cut(s) 178
SetI ASST 4 cut(s) 152, 157, 360, 447
SfcI CTRYAG 1 cut(s) 600
Sfr303I CCGCGG 1 cut(s) 577
SgrBI CCGCGG 1 cut(s) 577
SinI GGWCC 1 cut(s) 346
Sse9I AATT 1 cut(s) 368
SspMI CTAG 2 cut(s) 555, 616
StyD4I CCNGG 3 cut(s) 39, 57, 294
TaaI ACNGT 7 cut(s) 98, 110, 116, 122, 134, 331, 561
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 64
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 368
TatI WGTACW 1 cut(s) 561
TauI GCSGC 5 cut(s) 270, 273, 319, 576, 579
TfiI GAWTC 3 cut(s) 48, 469, 485
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TseFI GTSAC 7 cut(s) 83, 104, 110, 116, 122, 150, 538
TseI GCWGC 4 cut(s) 168, 285, 394, 408
Tsp45I GTSAC 7 cut(s) 83, 104, 110, 116, 122, 150, 538
TspDTI ATGAA 2 cut(s) 470, 581
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 1 cut(s) 368
XspI CTAG 2 cut(s) 555, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.