pycom13g22590

U4 U6.U5 small nuclear ribonucleoprotein 27 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010002
Physical Location & Seq
Reverse (-)
20198288 .. 20198824
537 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g22590.1

Sequence Viewer

Length: 537 bp
ATGGGGGACCGAGAACGAGACAGAGACCGGGACAGAGAAAGACGCCGAGACCGCGACCGCGTCCGCATCAAGAGCTCGCATACACCCGAGCGCACCAGACCACGCCACACCCGCTCCCGCACGCGCTCCATCTCCCGTTCTCGGACTCCAGACGACAGGTCCCACCGGCGGCGCCAGCACCACGACCGCAGCCCCTCGACGGAGCCACGCAAGCGGCAGCGCCGCGAGTCGACCGTGGAGGACGAGAGGGAGAGGCAGAAGGCTGCGGTTTTGGACTTGGTTGAGGGCATCACGAAGGAGCAGAAAACTCAGCAGAAGCCGAGCAGCGATGGTGATGGTGGTGAGGGTATGGACGAGGATGAGATTGAGATGATGAAGAAATTGGGGATTCCGACTGGGTTCGACTCGACAAAAGGGAAGCCCGTGCTGGGCGCCGATATAAGTGGCGTTAGGGCCGTCACGAAGCGGCAGCCAAGACAGTATATGAACCGCCGCGGTGGATTTAATCGGCCGTTGCCTGCCGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

179

Amino Acids

20.96

Weight (kDa)

11.16

Isoelectric Point (pI)

73.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 119 - 174 9.8e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 171, 431
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 230
AccII CGCG 5 cut(s) 54, 60, 124, 225, 495
AcoI YGGCCR 1 cut(s) 509
AcyI GRCGYC 3 cut(s) 43, 172, 432
AfiI CCNNNNNNNGG 4 cut(s) 141, 168, 199, 428
AhdI GACNNNNNGTC 1 cut(s) 157
AluBI AGCT 1 cut(s) 75
AluI AGCT 1 cut(s) 75
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 3 cut(s) 12, 18, 42
Ama87I CYCGRG 1 cut(s) 86
AoxI GGCC 2 cut(s) 453, 509
ApeKI GCWGC 5 cut(s) 189, 217, 263, 324, 469
AspLEI GCGC 6 cut(s) 93, 126, 174, 222, 434, 528
AspS9I GGNCC 3 cut(s) 7, 159, 453
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 344, 353
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 2 cut(s) 7, 159
BanI GGYRCC 2 cut(s) 171, 431
BanII GRGCYC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 5 cut(s) 201, 229, 250, 336, 481
BccI CCATC 3 cut(s) 137, 323, 329
BceAI ACGGC 2 cut(s) 440, 496
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 3 cut(s) 12, 18, 42
BfaI CTAG 1 cut(s) 535
BfoI RGCGCY 3 cut(s) 175, 223, 435
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 2 cut(s) 7, 159
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 3 cut(s) 7, 159, 453
BmiI GGNNCC 5 cut(s) 8, 161, 173, 204, 433
BmrFI CCNGG 1 cut(s) 29
BmrI ACTGGG 1 cut(s) 405
BmsI GCATC 2 cut(s) 75, 297
BmuI ACTGGG 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 132
BpuMI CCSGG 1 cut(s) 29
BsaHI GRCGYC 3 cut(s) 43, 172, 432
BsaI GGTCTC 2 cut(s) 18, 42
BsaJI CCNNGG 2 cut(s) 234, 493
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 168, 199, 428
Bse118I RCCGGY 1 cut(s) 165
Bse1I ACTGG 1 cut(s) 400
BseDI CCNNGG 2 cut(s) 234, 493
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 4 cut(s) 141, 168, 199, 428
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 400
BseX3I CGGCCG 1 cut(s) 509
BseXI GCAGC 5 cut(s) 201, 229, 250, 336, 481
BseYI CCCAGC 1 cut(s) 427
Bsh1236I CGCG 5 cut(s) 54, 60, 124, 225, 495
Bsh1285I CGRYCG 4 cut(s) 58, 187, 234, 512
BshFI GGCC 2 cut(s) 455, 511
BshNI GGYRCC 2 cut(s) 171, 431
BsiEI CGRYCG 4 cut(s) 58, 187, 234, 512
BsiHKAI GWGCWC 1 cut(s) 77
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 2 cut(s) 28, 166
BslFI GGGAC 3 cut(s) 20, 44, 145
BslI CCNNNNNNNGG 4 cut(s) 141, 168, 199, 428
BsmAI GTCTC 3 cut(s) 12, 18, 42
BsmFI GGGAC 3 cut(s) 20, 44, 145
BsnI GGCC 2 cut(s) 455, 511
Bso31I GGTCTC 2 cut(s) 18, 42
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 77
BspANI GGCC 2 cut(s) 455, 511
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 5 cut(s) 54, 60, 124, 225, 495
BspLI GGNNCC 5 cut(s) 8, 161, 173, 204, 433
BspT107I GGYRCC 2 cut(s) 171, 431
BspTNI GGTCTC 2 cut(s) 18, 42
BsrBI CCGCTC 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 165
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 234, 493
BssNI GRCGYC 3 cut(s) 43, 172, 432
Bst4CI ACNGT 2 cut(s) 235, 480
BstACI GRCGYC 3 cut(s) 43, 172, 432
BstC8I GCNNGC 5 cut(s) 77, 122, 176, 212, 519
BstDEI CTNAG 1 cut(s) 309
BstDSI CCRYGG 2 cut(s) 234, 493
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 5 cut(s) 54, 60, 124, 225, 495
BstH2I RGCGCY 3 cut(s) 175, 223, 435
BstHHI GCGC 6 cut(s) 93, 126, 174, 222, 434, 528
BstMAI GTCTC 3 cut(s) 12, 18, 42
BstMCI CGRYCG 4 cut(s) 58, 187, 234, 512
BstMWI GCNNNNNNNGC 6 cut(s) 51, 72, 111, 175, 211, 523
BstSCI CCNGG 1 cut(s) 27
BstUI CGCG 5 cut(s) 54, 60, 124, 225, 495
BstV1I GCAGC 5 cut(s) 201, 229, 250, 336, 481
BstZI CGGCCG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 455, 511
BtgI CCRYGG 2 cut(s) 234, 493
BtgZI GCGATG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 364
Cac8I GCNNGC 5 cut(s) 77, 122, 176, 212, 519
CfoI GCGC 6 cut(s) 93, 126, 174, 222, 434, 528
Cfr10I RCCGGY 1 cut(s) 165
Cfr13I GGNCC 3 cut(s) 7, 159, 453
Cfr42I CCGCGG 1 cut(s) 496
CseI GACGC 2 cut(s) 49, 51
CviJI RGCY 9 cut(s) 75, 192, 205, 263, 319, 421, 455, 472, 511
CviKI_1 RGCY 9 cut(s) 75, 192, 205, 263, 319, 421, 455, 472, 511
DdeI CTNAG 1 cut(s) 309
DinI GGCGCC 2 cut(s) 173, 433
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 509
EagI CGGCCG 1 cut(s) 509
Eam1105I GACNNNNNGTC 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 75
EclXI CGGCCG 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 77
Eco31I GGTCTC 2 cut(s) 18, 42
Eco47I GGWCC 2 cut(s) 7, 159
Eco52I CGGCCG 1 cut(s) 509
Eco53kI GAGCTC 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 77
EgeI GGCGCC 2 cut(s) 173, 433
EheI GGCGCC 2 cut(s) 173, 433
FaiI YATR 5 cut(s) 81, 350, 440, 483, 485
FaqI GGGAC 3 cut(s) 20, 44, 145
FauI CCCGC 2 cut(s) 119, 125
FblI GTMKAC 1 cut(s) 230
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 1 cut(s) 77
FspBI CTAG 1 cut(s) 535
GlaI GCGC 6 cut(s) 92, 125, 173, 221, 433, 527
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 1 cut(s) 132
HaeII RGCGCY 3 cut(s) 175, 223, 435
HaeIII GGCC 2 cut(s) 455, 511
HapII CCGG 2 cut(s) 28, 166
HgaI GACGC 2 cut(s) 49, 51
HhaI GCGC 6 cut(s) 93, 126, 174, 222, 434, 528
Hin1I GRCGYC 3 cut(s) 43, 172, 432
Hin6I GCGC 6 cut(s) 91, 124, 172, 220, 432, 526
HinP1I GCGC 6 cut(s) 91, 124, 172, 220, 432, 526
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 4 cut(s) 145, 227, 388, 404
HpaII CCGG 2 cut(s) 28, 166
HphI GGTGA 2 cut(s) 344, 353
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 2 cut(s) 144, 393
Hpy188III TCNNGA 4 cut(s) 70, 149, 292, 460
Hpy8I GTNNAC 1 cut(s) 231
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 253, 289
HpyCH4III ACNGT 2 cut(s) 235, 480
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 72, 111, 175, 211, 523
HpyF3I CTNAG 1 cut(s) 309
Hsp92I GRCGYC 3 cut(s) 43, 172, 432
HspAI GCGC 6 cut(s) 91, 124, 172, 220, 432, 526
KasI GGCGCC 2 cut(s) 171, 431
KspI CCGCGG 1 cut(s) 496
LmnI GCTCC 4 cut(s) 119, 131, 202, 298
LpnPI CCDG 9 cut(s) 41, 109, 142, 162, 179, 188, 381, 413, 531
Lsp1109I GCAGC 5 cut(s) 201, 229, 250, 336, 481
LweI GCATC 2 cut(s) 75, 297
MaeI CTAG 1 cut(s) 535
MaeIII GTNAC 1 cut(s) 457
MbiI CCGCTC 1 cut(s) 114
MboII GAAGA 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 1 cut(s) 380
Mly113I GGCGCC 2 cut(s) 172, 432
MlyI GAGTC 3 cut(s) 139, 236, 398
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 7 cut(s) 205, 232, 240, 246, 277, 337, 349
MseI TTAA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 495
MspI CCGG 2 cut(s) 28, 166
MspR9I CCNGG 1 cut(s) 29
MvnI CGCG 5 cut(s) 54, 60, 124, 225, 495
MwoI GCNNNNNNNGC 6 cut(s) 51, 72, 111, 175, 211, 523
NarI GGCGCC 2 cut(s) 172, 432
NciI CCSGG 1 cut(s) 29
NlaIV GGNNCC 5 cut(s) 8, 161, 173, 204, 433
NmeAIII GCCGAG 2 cut(s) 71, 345
NmuCI GTSAC 1 cut(s) 457
PfeI GAWTC 1 cut(s) 388
PflFI GACNNNGTC 1 cut(s) 59
PleI GAGTC 3 cut(s) 139, 235, 398
PluTI GGCGCC 2 cut(s) 175, 435
PpsI GAGTC 3 cut(s) 139, 235, 398
PpuMI RGGWCCY 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 427
PspN4I GGNNCC 5 cut(s) 8, 161, 173, 204, 433
PspPI GGNCC 3 cut(s) 7, 159, 453
PspPPI RGGWCCY 1 cut(s) 159
PsyI GACNNNGTC 1 cut(s) 59
SacI GAGCTC 1 cut(s) 77
SacII CCGCGG 1 cut(s) 496
SalI GTCGAC 1 cut(s) 229
SaqAI TTAA 1 cut(s) 504
Sau96I GGNCC 3 cut(s) 7, 159, 453
SchI GAGTC 3 cut(s) 139, 236, 398
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 1 cut(s) 77
SetI ASST 2 cut(s) 77, 161
SfaNI GCATC 2 cut(s) 75, 297
SfoI GGCGCC 2 cut(s) 173, 433
Sfr303I CCGCGG 1 cut(s) 496
SgrAI CRCCGGYG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 496
SinI GGWCC 2 cut(s) 7, 159
Sse9I AATT 1 cut(s) 380
SspDI GGCGCC 2 cut(s) 171, 431
SspMI CTAG 1 cut(s) 535
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 235, 480
TaqI TCGA 4 cut(s) 197, 230, 402, 407
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 380
TauI GCSGC 5 cut(s) 172, 217, 225, 469, 495
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 5 cut(s) 189, 217, 263, 324, 469
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 389, 500
TspGWI ACGGA 1 cut(s) 215
Tth111I GACNNNGTC 1 cut(s) 59
VpaK11BI GGWCC 2 cut(s) 7, 159
XmiI GTMKAC 1 cut(s) 230
XspI CTAG 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.