FvH4_4g07990

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010002
Physical Location & Seq
Forward (+)
7575451 .. 7579127
3677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07990.t1

Sequence Viewer

Length: 612 bp
ATGGCAGACCGAGAAAGAGAGCGAGACAGAGACCGTGACCGTGAGAGAATCCGAGACCGCGACCGAGACAGAGACCGACGCCGTGACCGTGACGACCGTGATCGCGACCGTGACCGTGACCGTGACCGTGTCCGGAGCAAGAGGTCGCGTACGCCGGAGCGCACCAGAGCACGCCACACTCGCTCCCGCACGCGCTCGCCGGACCAATACCACTCACGCTCCCTCTCCCGCTCCCGCACTCCCGAAGACCGCTCCCACCGGCGCCGCCACCACCACCACCGCAGCCCCTCCCCGGAGTCGCGGAAGCGAGCTCGGCGCGAGCCGACGGTAGAGGCCGAGAAGGACCGGCAGAAGGCGGTGTCGGAATTCGTGGAGGGGGTTGCGAAGGAGCAGCCGAACAACGGTGGCGGAGAGGGCGGCGGTGCGGACGAGGACGAGATTGAGATGATGAAGAAGCTAGGGATTCCGGTAGGGTTTGATTCTACGAAAGGGAAGCCGGTGCCCGGCGCCGATGTGAGTGGTGTTAGGGCCGTCACGAAGCGCCAGCCGAGACAGTACATGAACCGCCGCGGTGGATTTAACCGGCCATTGCCTGCAGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

204

Amino Acids

24.13

Weight (kDa)

11.31

Isoelectric Point (pI)

79.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 144 - 199 2.6e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 261, 499, 506
AccBSI CCGCTC 2 cut(s) 231, 252
AccII CGCG 7 cut(s) 60, 105, 148, 193, 301, 318, 570
AccIII TCCGGA 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 1 cut(s) 365
AcyI GRCGYC 3 cut(s) 79, 262, 507
AfaI GTAC 2 cut(s) 151, 557
AfiI CCNNNNNNNGG 4 cut(s) 292, 352, 401, 503
AluBI AGCT 2 cut(s) 311, 457
AluI AGCT 2 cut(s) 311, 457
Alw21I GWGCWC 2 cut(s) 172, 313
Alw26I GTCTC 6 cut(s) 18, 24, 48, 60, 66, 544
Aor13HI TCCGGA 1 cut(s) 132
AoxI GGCC 3 cut(s) 333, 528, 584
ApeKI GCWGC 2 cut(s) 282, 391
ApoI RAATTY 1 cut(s) 365
AspLEI GCGC 7 cut(s) 162, 195, 264, 318, 509, 543, 603
AspS9I GGNCC 3 cut(s) 202, 343, 528
AsuC2I CCSGG 2 cut(s) 293, 504
AvaII GGWCC 2 cut(s) 202, 343
BaeGI GKGCMC 1 cut(s) 504
BanI GGYRCC 3 cut(s) 261, 499, 506
BanII GRGCYC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 172, 313
BbvI GCAGC 2 cut(s) 294, 403
BceAI ACGGC 2 cut(s) 66, 515
BcnI CCSGG 2 cut(s) 293, 504
BcoDI GTCTC 6 cut(s) 18, 24, 48, 60, 66, 544
BfaI CTAG 2 cut(s) 458, 610
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 3 cut(s) 265, 510, 544
BisI GCNGC 5 cut(s) 265, 283, 392, 418, 568
BlsI GCNGC 5 cut(s) 266, 284, 393, 419, 569
Bme1390I CCNGG 2 cut(s) 293, 504
Bme18I GGWCC 2 cut(s) 202, 343
BmgT120I GGNCC 3 cut(s) 202, 343, 528
BmiI GGNNCC 3 cut(s) 263, 501, 508
BmrFI CCNGG 2 cut(s) 293, 504
BpiI GAAGAC 1 cut(s) 252
BpuMI CCSGG 2 cut(s) 293, 504
BsaHI GRCGYC 3 cut(s) 79, 262, 507
BsaI GGTCTC 3 cut(s) 24, 48, 66
BsaJI CCNNGG 2 cut(s) 291, 568
BsaWI WCCGGW 2 cut(s) 132, 466
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 203, 233
Bsc4I CCNNNNNNNGG 4 cut(s) 292, 352, 401, 503
Bse118I RCCGGY 4 cut(s) 258, 345, 496, 582
Bse3DI GCAATG 1 cut(s) 587
BseAI TCCGGA 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 291, 568
BseLI CCNNNNNNNGG 4 cut(s) 292, 352, 401, 503
BseMI GCAATG 1 cut(s) 587
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 2 cut(s) 294, 403
Bsh1236I CGCG 7 cut(s) 60, 105, 148, 193, 301, 318, 570
Bsh1285I CGRYCG 3 cut(s) 64, 97, 109
BshFI GGCC 3 cut(s) 335, 530, 586
BshNI GGYRCC 3 cut(s) 261, 499, 506
BsiEI CGRYCG 3 cut(s) 64, 97, 109
BsiHKAI GWGCWC 2 cut(s) 172, 313
BsiWI CGTACG 1 cut(s) 149
BslI CCNNNNNNNGG 4 cut(s) 292, 352, 401, 503
BsmAI GTCTC 6 cut(s) 18, 24, 48, 60, 66, 544
BsnI GGCC 3 cut(s) 335, 530, 586
Bso31I GGTCTC 3 cut(s) 24, 48, 66
Bsp1286I GDGCHC 3 cut(s) 172, 313, 504
Bsp13I TCCGGA 1 cut(s) 132
Bsp143I GATC 1 cut(s) 100
Bsp68I TCGCGA 1 cut(s) 105
BspANI GGCC 3 cut(s) 335, 530, 586
BspEI TCCGGA 1 cut(s) 132
BspFNI CGCG 7 cut(s) 60, 105, 148, 193, 301, 318, 570
BspLI GGNNCC 3 cut(s) 263, 501, 508
BspMAI CTGCAG 1 cut(s) 598
BspT107I GGYRCC 3 cut(s) 261, 499, 506
BspTNI GGTCTC 3 cut(s) 24, 48, 66
BsrBI CCGCTC 2 cut(s) 231, 252
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 4 cut(s) 258, 345, 496, 582
BssAI RCCGGY 4 cut(s) 258, 345, 496, 582
BssECI CCNNGG 2 cut(s) 291, 568
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 3 cut(s) 79, 262, 507
BstACI GRCGYC 3 cut(s) 79, 262, 507
BstC8I GCNNGC 7 cut(s) 172, 191, 197, 309, 320, 545, 594
BstDSI CCRYGG 1 cut(s) 568
BstFNI CGCG 7 cut(s) 60, 105, 148, 193, 301, 318, 570
BstH2I RGCGCY 3 cut(s) 265, 510, 544
BstHHI GCGC 7 cut(s) 162, 195, 264, 318, 509, 543, 603
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 6 cut(s) 18, 24, 48, 60, 66, 544
BstMBI GATC 1 cut(s) 100
BstMCI CGRYCG 3 cut(s) 64, 97, 109
BstMWI GCNNNNNNNGC 4 cut(s) 180, 313, 414, 598
BstSCI CCNGG 2 cut(s) 291, 502
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 7 cut(s) 60, 105, 148, 193, 301, 318, 570
BstV1I GCAGC 2 cut(s) 294, 403
BstV2I GAAGAC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 335, 530, 586
BtgI CCRYGG 1 cut(s) 568
BtuMI TCGCGA 1 cut(s) 105
Cac8I GCNNGC 7 cut(s) 172, 191, 197, 309, 320, 545, 594
CfoI GCGC 7 cut(s) 162, 195, 264, 318, 509, 543, 603
Cfr10I RCCGGY 4 cut(s) 258, 345, 496, 582
Cfr13I GGNCC 3 cut(s) 202, 343, 528
Cfr42I CCGCGG 1 cut(s) 571
CseI GACGC 1 cut(s) 87
Csp6I GTAC 2 cut(s) 150, 556
CviAII CATG 1 cut(s) 559
CviQI GTAC 2 cut(s) 150, 556
DinI GGCGCC 2 cut(s) 263, 508
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 584
EciI GGCGGA 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 313
Eco31I GGTCTC 3 cut(s) 24, 48, 66
Eco47I GGWCC 2 cut(s) 202, 343
Eco53kI GAGCTC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 313
EgeI GGCGCC 2 cut(s) 263, 508
EheI GGCGCC 2 cut(s) 263, 508
FaeI CATG 1 cut(s) 562
FaiI YATR 1 cut(s) 560
FatI CATG 1 cut(s) 558
FauI CCCGC 3 cut(s) 194, 236, 242
Fnu4HI GCNGC 5 cut(s) 265, 283, 392, 418, 568
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 265, 283, 392, 418, 568
FspBI CTAG 2 cut(s) 458, 610
GlaI GCGC 7 cut(s) 161, 194, 263, 317, 508, 542, 602
GluI GCNGC 5 cut(s) 265, 283, 392, 418, 568
HaeII RGCGCY 3 cut(s) 265, 510, 544
HaeIII GGCC 3 cut(s) 335, 530, 586
HgaI GACGC 1 cut(s) 87
HhaI GCGC 7 cut(s) 162, 195, 264, 318, 509, 543, 603
Hin1I GRCGYC 3 cut(s) 79, 262, 507
Hin1II CATG 1 cut(s) 562
Hin6I GCGC 7 cut(s) 160, 193, 262, 316, 507, 541, 601
HinP1I GCGC 7 cut(s) 160, 193, 262, 316, 507, 541, 601
HinfI GANTC 4 cut(s) 48, 296, 463, 479
Hpy188I TCNGA 2 cut(s) 53, 364
Hpy188III TCNNGA 4 cut(s) 104, 133, 242, 535
Hpy99I CGWCG 2 cut(s) 81, 328
HpyAV CCTTC 3 cut(s) 334, 346, 379
HpyCH4V TGCA 1 cut(s) 596
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 313, 414, 598
Hsp92I GRCGYC 3 cut(s) 79, 262, 507
Hsp92II CATG 1 cut(s) 562
HspAI GCGC 7 cut(s) 160, 193, 262, 316, 507, 541, 601
KasI GGCGCC 2 cut(s) 261, 506
Kpn2I TCCGGA 1 cut(s) 132
KspI CCGCGG 1 cut(s) 571
Kzo9I GATC 1 cut(s) 100
LmnI GCTCC 7 cut(s) 135, 157, 188, 224, 236, 257, 388
Lsp1109I GCAGC 2 cut(s) 294, 403
MaeI CTAG 2 cut(s) 458, 610
MaeIII GTNAC 7 cut(s) 35, 83, 89, 110, 116, 122, 532
MalI GATC 1 cut(s) 102
MbiI CCGCTC 2 cut(s) 231, 252
MboI GATC 1 cut(s) 100
MboII GAAGA 2 cut(s) 257, 463
MhlI GDGCHC 3 cut(s) 172, 313, 504
MluCI AATT 1 cut(s) 365
Mly113I GGCGCC 2 cut(s) 262, 507
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 7 cut(s) 135, 233, 298, 325, 367, 406, 424
MroI TCCGGA 1 cut(s) 132
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 570
MspR9I CCNGG 2 cut(s) 293, 504
MvnI CGCG 7 cut(s) 60, 105, 148, 193, 301, 318, 570
MwoI GCNNNNNNNGC 4 cut(s) 180, 313, 414, 598
NarI GGCGCC 2 cut(s) 262, 507
NciI CCSGG 2 cut(s) 293, 504
NdeII GATC 1 cut(s) 100
NlaIII CATG 1 cut(s) 562
NlaIV GGNNCC 3 cut(s) 263, 501, 508
NmeAIII GCCGAG 3 cut(s) 292, 361, 573
NmuCI GTSAC 7 cut(s) 35, 83, 89, 110, 116, 122, 532
NruI TCGCGA 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PfeI GAWTC 3 cut(s) 48, 463, 479
Pfl23II CGTACG 1 cut(s) 149
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 5 cut(s) 266, 284, 393, 419, 569
PleI GAGTC 1 cut(s) 304
PluTI GGCGCC 2 cut(s) 265, 510
PpsI GAGTC 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 313
PspLI CGTACG 1 cut(s) 149
PspN4I GGNNCC 3 cut(s) 263, 501, 508
PspPI GGNCC 3 cut(s) 202, 343, 528
PstI CTGCAG 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 128
RruI TCGCGA 1 cut(s) 105
RsaI GTAC 2 cut(s) 151, 557
RsaNI GTAC 2 cut(s) 150, 556
SacI GAGCTC 1 cut(s) 313
SacII CCGCGG 1 cut(s) 571
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 5 cut(s) 265, 283, 392, 418, 568
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 3 cut(s) 202, 343, 528
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 293, 504
SduI GDGCHC 3 cut(s) 172, 313, 504
SetI ASST 3 cut(s) 146, 313, 459
SfcI CTRYAG 1 cut(s) 594
SfoI GGCGCC 2 cut(s) 263, 508
Sfr303I CCGCGG 1 cut(s) 571
SgrAI CRCCGGYG 1 cut(s) 258
SgrBI CCGCGG 1 cut(s) 571
SinI GGWCC 2 cut(s) 202, 343
Sse9I AATT 1 cut(s) 365
SspDI GGCGCC 2 cut(s) 261, 506
SspMI CTAG 2 cut(s) 458, 610
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 2 cut(s) 291, 502
TaqII GACCGA 3 cut(s) 24, 78, 90
TasI AATT 1 cut(s) 365
TatI WGTACW 1 cut(s) 555
TauI GCSGC 3 cut(s) 267, 420, 570
TfiI GAWTC 3 cut(s) 48, 463, 479
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TseFI GTSAC 7 cut(s) 35, 83, 89, 110, 116, 122, 532
TseI GCWGC 2 cut(s) 282, 391
Tsp45I GTSAC 7 cut(s) 35, 83, 89, 110, 116, 122, 532
TspDTI ATGAA 2 cut(s) 464, 575
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 202, 343
XapI RAATTY 1 cut(s) 365
XspI CTAG 2 cut(s) 458, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.