Rroxscaffold_5G00346190

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010002
Physical Location & Seq
Forward (+)
16944470 .. 16945090
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00346190.1

Sequence Viewer

Length: 621 bp
ATGGGAGACCGAGAAAGAGAGCGAGACAGAGAACGAGATCGGGAGAGAATCCGAGACCGAGACCGGGACCGGGACCGAGACAGACGCCGTGACAGAGACGACCGTGATCGTGATCGTGACCGTGACCGGGACCGTGTCCGGAGCAAGAGGTCACGCACGCCGGAGCGCAGCAGAGCACGCCACACGCGCTCCCGCACGCGCTCGCCGGACTTGTACCACTCACGCTCCCTCTCCCGTTCCCGCACTCCAGAAGACCGCTCCCACCGCCGCCGCCACCACCACCGCAGCCCCTCCCCGGAGTCGCGGAAGCGTGCGCGGCGCGAGCCGACGGTGGAGGAGGAGAAGGACCGGCAGAAGGTGGTGTCGGAATTCGTGGAGGGGATTGCGAAGGAGCAGCAGAAGCAGAGCAGCGGTGGTGGTGGAGAGGGCGGTGCCGACGAGGACGAGGTTGAGATGATGAAGAAGTTGGGGATTCCGGTAGGGTTTGATTCGACGAAGGGGAAGCCGGTGCCGGGGGCCGATGTGAGTGGTGTTAGGGCAGTCACGAAGCGCCAGCCTAGACAGTACATGAACCGCCGCGGCGGATTTAACCGGCCATTGCCTGCAGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

206

Amino Acids

24.55

Weight (kDa)

11.24

Isoelectric Point (pI)

85.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 147 - 202 2.7e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 431, 508
AccBSI CCGCTC 1 cut(s) 258
AccII CGCG 6 cut(s) 187, 199, 304, 316, 321, 579
AccIII TCCGGA 1 cut(s) 138
AcoI YGGCCR 1 cut(s) 593
AcsI RAATTY 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 2 cut(s) 215, 566
AfiI CCNNNNNNNGG 6 cut(s) 64, 70, 127, 295, 355, 512
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 5 cut(s) 18, 48, 54, 72, 90
Aor13HI TCCGGA 1 cut(s) 138
AoxI GGCC 2 cut(s) 516, 593
ApeKI GCWGC 4 cut(s) 168, 285, 394, 408
ApoI RAATTY 1 cut(s) 368
AspLEI GCGC 7 cut(s) 168, 189, 201, 316, 321, 552, 612
AspS9I GGNCC 5 cut(s) 67, 73, 130, 346, 516
AsuC2I CCSGG 5 cut(s) 65, 71, 128, 296, 513
AvaII GGWCC 4 cut(s) 67, 73, 130, 346
BanI GGYRCC 2 cut(s) 431, 508
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 4 cut(s) 180, 297, 406, 420
BceAI ACGGC 1 cut(s) 72
BcnI CCSGG 5 cut(s) 65, 71, 128, 296, 513
BcoDI GTCTC 5 cut(s) 18, 48, 54, 72, 90
BfaI CTAG 2 cut(s) 558, 619
BfmI CTRYAG 1 cut(s) 603
BfoI RGCGCY 1 cut(s) 553
BisI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 577, 580
BlsI GCNGC 9 cut(s) 170, 269, 272, 287, 318, 396, 410, 578, 581
Bme1390I CCNGG 5 cut(s) 65, 71, 128, 296, 513
Bme18I GGWCC 4 cut(s) 67, 73, 130, 346
BmgT120I GGNCC 5 cut(s) 67, 73, 130, 346, 516
BmiI GGNNCC 6 cut(s) 68, 74, 131, 433, 510, 517
BmrFI CCNGG 5 cut(s) 65, 71, 128, 296, 513
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 231
BpuMI CCSGG 5 cut(s) 65, 71, 128, 296, 513
BsaBI GATNNNNATC 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 85
BsaI GGTCTC 2 cut(s) 48, 54
BsaJI CCNNGG 3 cut(s) 294, 512, 577
BsaWI WCCGGW 2 cut(s) 138, 475
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 209, 239
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 70, 127, 295, 355, 512
Bse118I RCCGGY 3 cut(s) 348, 505, 591
Bse3DI GCAATG 1 cut(s) 596
Bse8I GATNNNNATC 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 294, 512, 577
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 6 cut(s) 64, 70, 127, 295, 355, 512
BseMI GCAATG 1 cut(s) 596
BseRI GAGGAG 2 cut(s) 350, 353
BseXI GCAGC 4 cut(s) 180, 297, 406, 420
Bsh1236I CGCG 6 cut(s) 187, 199, 304, 316, 321, 579
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 2 cut(s) 518, 595
BshNI GGYRCC 2 cut(s) 431, 508
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 178
BslFI GGGAC 3 cut(s) 80, 86, 143
BslI CCNNNNNNNGG 6 cut(s) 64, 70, 127, 295, 355, 512
BsmAI GTCTC 5 cut(s) 18, 48, 54, 72, 90
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 3 cut(s) 80, 86, 143
BsnI GGCC 2 cut(s) 518, 595
Bso31I GGTCTC 2 cut(s) 48, 54
Bsp1286I GDGCHC 1 cut(s) 178
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 37, 106, 112
BspANI GGCC 2 cut(s) 518, 595
BspEI TCCGGA 1 cut(s) 138
BspFNI CGCG 6 cut(s) 187, 199, 304, 316, 321, 579
BspLI GGNNCC 6 cut(s) 68, 74, 131, 433, 510, 517
BspMAI CTGCAG 1 cut(s) 607
BspT107I GGYRCC 2 cut(s) 431, 508
BspTNI GGTCTC 2 cut(s) 48, 54
BsrBI CCGCTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 3 cut(s) 348, 505, 591
BssAI RCCGGY 3 cut(s) 348, 505, 591
BssECI CCNNGG 3 cut(s) 294, 512, 577
BssMI GATC 3 cut(s) 37, 106, 112
BssNI GRCGYC 1 cut(s) 85
Bst4CI ACNGT 5 cut(s) 104, 122, 134, 331, 564
BstACI GRCGYC 1 cut(s) 85
BstC8I GCNNGC 8 cut(s) 158, 178, 197, 203, 312, 323, 554, 603
BstDSI CCRYGG 1 cut(s) 577
BstFNI CGCG 6 cut(s) 187, 199, 304, 316, 321, 579
BstH2I RGCGCY 1 cut(s) 553
BstHHI GCGC 7 cut(s) 168, 189, 201, 316, 321, 552, 612
BstKTI GATC 3 cut(s) 40, 109, 115
BstMAI GTCTC 5 cut(s) 18, 48, 54, 72, 90
BstMBI GATC 3 cut(s) 37, 106, 112
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 7 cut(s) 177, 186, 264, 316, 322, 400, 607
BstSCI CCNGG 5 cut(s) 63, 69, 126, 294, 511
BstSFI CTRYAG 1 cut(s) 603
BstUI CGCG 6 cut(s) 187, 199, 304, 316, 321, 579
BstV1I GCAGC 4 cut(s) 180, 297, 406, 420
BstV2I GAAGAC 1 cut(s) 258
BsuRI GGCC 2 cut(s) 518, 595
BtgI CCRYGG 1 cut(s) 577
Cac8I GCNNGC 8 cut(s) 158, 178, 197, 203, 312, 323, 554, 603
CfoI GCGC 7 cut(s) 168, 189, 201, 316, 321, 552, 612
Cfr10I RCCGGY 3 cut(s) 348, 505, 591
Cfr13I GGNCC 5 cut(s) 67, 73, 130, 346, 516
Cfr42I CCGCGG 1 cut(s) 580
CseI GACGC 1 cut(s) 93
Csp6I GTAC 2 cut(s) 214, 565
CviAII CATG 1 cut(s) 568
CviJI RGCY 6 cut(s) 288, 325, 505, 518, 556, 595
CviKI_1 RGCY 6 cut(s) 288, 325, 505, 518, 556, 595
CviQI GTAC 2 cut(s) 214, 565
DpnI GATC 3 cut(s) 39, 108, 114
DpnII GATC 3 cut(s) 37, 106, 112
EaeI YGGCCR 1 cut(s) 593
EciI GGCGGA 1 cut(s) 597
Eco31I GGTCTC 2 cut(s) 48, 54
Eco47I GGWCC 4 cut(s) 67, 73, 130, 346
EcoRI GAATTC 1 cut(s) 368
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 1 cut(s) 571
FaiI YATR 1 cut(s) 569
FaqI GGGAC 3 cut(s) 80, 86, 143
FatI CATG 1 cut(s) 567
FauI CCCGC 2 cut(s) 200, 248
Fnu4HI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 577, 580
Fsp4HI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 577, 580
FspBI CTAG 2 cut(s) 558, 619
GlaI GCGC 7 cut(s) 167, 188, 200, 315, 320, 551, 611
GluI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 577, 580
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 553
HaeIII GGCC 2 cut(s) 518, 595
HgaI GACGC 1 cut(s) 93
HhaI GCGC 7 cut(s) 168, 189, 201, 316, 321, 552, 612
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 7 cut(s) 166, 187, 199, 314, 319, 550, 610
HinP1I GCGC 7 cut(s) 166, 187, 199, 314, 319, 550, 610
HinfI GANTC 4 cut(s) 48, 299, 472, 488
Hpy188I TCNGA 2 cut(s) 53, 367
Hpy188III TCNNGA 6 cut(s) 41, 110, 116, 139, 248, 544
Hpy99I CGWCG 3 cut(s) 331, 440, 496
HpyAV CCTTC 4 cut(s) 337, 349, 382, 490
HpyCH4III ACNGT 5 cut(s) 104, 122, 134, 331, 564
HpyCH4V TGCA 1 cut(s) 605
HpyF10VI GCNNNNNNNGC 7 cut(s) 177, 186, 264, 316, 322, 400, 607
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 7 cut(s) 166, 187, 199, 314, 319, 550, 610
Kpn2I TCCGGA 1 cut(s) 138
KspI CCGCGG 1 cut(s) 580
Kzo9I GATC 3 cut(s) 37, 106, 112
LmnI GCTCC 6 cut(s) 141, 163, 194, 230, 263, 391
Lsp1109I GCAGC 4 cut(s) 180, 297, 406, 420
MaeI CTAG 2 cut(s) 558, 619
MaeIII GTNAC 5 cut(s) 89, 116, 122, 150, 541
MalI GATC 3 cut(s) 39, 108, 114
MbiI CCGCTC 1 cut(s) 258
MboI GATC 3 cut(s) 37, 106, 112
MboII GAAGA 2 cut(s) 263, 472
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 1 cut(s) 368
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 9 cut(s) 141, 239, 301, 328, 331, 370, 418, 433, 439
MroI TCCGGA 1 cut(s) 138
MseI TTAA 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 411, 579
MspR9I CCNGG 5 cut(s) 65, 71, 128, 296, 513
MteI GCGCNGCGC 1 cut(s) 317
MvnI CGCG 6 cut(s) 187, 199, 304, 316, 321, 579
MwoI GCNNNNNNNGC 7 cut(s) 177, 186, 264, 316, 322, 400, 607
NciI CCSGG 5 cut(s) 65, 71, 128, 296, 513
NdeII GATC 3 cut(s) 37, 106, 112
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 6 cut(s) 68, 74, 131, 433, 510, 517
NmuCI GTSAC 5 cut(s) 89, 116, 122, 150, 541
PfeI GAWTC 3 cut(s) 48, 472, 488
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 9 cut(s) 170, 269, 272, 287, 318, 396, 410, 578, 581
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PspN4I GGNNCC 6 cut(s) 68, 74, 131, 433, 510, 517
PspPI GGNCC 5 cut(s) 67, 73, 130, 346, 516
PstI CTGCAG 1 cut(s) 607
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 2 cut(s) 215, 566
RsaNI GTAC 2 cut(s) 214, 565
SacII CCGCGG 1 cut(s) 580
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 9 cut(s) 169, 268, 271, 286, 317, 395, 409, 577, 580
Sau3AI GATC 3 cut(s) 37, 106, 112
Sau96I GGNCC 5 cut(s) 67, 73, 130, 346, 516
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 5 cut(s) 65, 71, 128, 296, 513
SduI GDGCHC 1 cut(s) 178
SetI ASST 3 cut(s) 152, 360, 450
SfcI CTRYAG 1 cut(s) 603
Sfr303I CCGCGG 1 cut(s) 580
SgrBI CCGCGG 1 cut(s) 580
SinI GGWCC 4 cut(s) 67, 73, 130, 346
Sse9I AATT 1 cut(s) 368
SspMI CTAG 2 cut(s) 558, 619
StyD4I CCNGG 5 cut(s) 63, 69, 126, 294, 511
TaaI ACNGT 5 cut(s) 104, 122, 134, 331, 564
TaqI TCGA 1 cut(s) 491
TaqII GACCGA 3 cut(s) 24, 72, 90
TasI AATT 1 cut(s) 368
TatI WGTACW 1 cut(s) 564
TauI GCSGC 5 cut(s) 270, 273, 319, 579, 582
TfiI GAWTC 3 cut(s) 48, 472, 488
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TseFI GTSAC 5 cut(s) 89, 116, 122, 150, 541
TseI GCWGC 4 cut(s) 168, 285, 394, 408
Tsp45I GTSAC 5 cut(s) 89, 116, 122, 150, 541
TspDTI ATGAA 2 cut(s) 473, 584
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 4 cut(s) 67, 73, 130, 346
XapI RAATTY 1 cut(s) 368
XspI CTAG 2 cut(s) 558, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.