MD16G1256400.v1.1

U4 U6.U5 small nuclear ribonucleoprotein 27 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010002
Physical Location & Seq
Forward (+)
29589166 .. 29590069
904 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1256400.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGGGGACCGAGAACGAGACAGGGACCGGGATCGTGACCGAATTCGGGACAGAGACCGTGACCGAGAAAGACGCCGAGAGCGAGACGACCGCGACCGTGACCGCGTCCGCATCAAGAGTTCACGTACACCTGAACGCACCAGACCGCGCCACACCCGCTCCCCCACGCGCTCGCCCGACCGGTACCGCTCCCGCACTCGCTCCATCTCCCGTTCTCGGTCTCCAGACGACAGGTCCCACCGGCGGCGCCAGCACCACCACCGCAGCCCCTCGCCAGAGCCACGCAAGCGCCAGCGCCGTGAGTCGACTGTAGAGGACGAGAGGGAGCGGCAGAAGGCGGCGGTTTCGAACTCCGTTGAGGGCATCTCGAAGGAGCATAAAACTCAGCAGAAGCCGAGCAGCGATGGTGGTGGTGGTGAGGGTATGGACGAGGACGAAATTGAGATGATGAAGAAATTGGGGATTCCGATTGGGTTCGACTCGACAAAAGGGAAGCCAGTGGCGGGCGCAGATGTGAGTGGCGTTAGGGCGGTCACTAAGCGGCAGCCGAGACAGTATATGAATCGCCGCGGTGGATTTAATCGGCCGTTGCCTGCCGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

204

Amino Acids

24.06

Weight (kDa)

11.38

Isoelectric Point (pI)

79.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 144 - 199 2.3e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 183
AccB1I GGYRCC 2 cut(s) 183, 246
AccBSI CCGCTC 3 cut(s) 159, 189, 328
AccI GTMKAC 1 cut(s) 305
AccII CGCG 5 cut(s) 93, 105, 148, 169, 570
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 1 cut(s) 42
AcyI GRCGYC 2 cut(s) 73, 247
AfaI GTAC 2 cut(s) 127, 185
AfiI CCNNNNNNNGG 4 cut(s) 46, 216, 243, 503
AgeI ACCGGT 1 cut(s) 180
AhdI GACNNNNNGTC 1 cut(s) 232
Alw26I GTCTC 5 cut(s) 12, 48, 78, 225, 544
AlwI GGATC 1 cut(s) 39
AoxI GGCC 1 cut(s) 584
ApeKI GCWGC 3 cut(s) 264, 399, 544
ApoI RAATTY 1 cut(s) 42
AsiGI ACCGGT 1 cut(s) 180
Asp718I GGTACC 1 cut(s) 183
AspLEI GCGC 7 cut(s) 150, 171, 249, 291, 297, 509, 603
AspS9I GGNCC 3 cut(s) 7, 25, 234
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 1 cut(s) 428
AsuII TTCGAA 1 cut(s) 347
AvaII GGWCC 3 cut(s) 7, 25, 234
BanI GGYRCC 2 cut(s) 183, 246
BbvI GCAGC 3 cut(s) 276, 411, 556
BccI CCATC 2 cut(s) 212, 398
BceAI ACGGC 2 cut(s) 282, 571
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 5 cut(s) 12, 48, 78, 225, 544
BfaI CTAG 1 cut(s) 610
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 3 cut(s) 250, 292, 298
BisI GCNGC 8 cut(s) 245, 265, 329, 339, 400, 542, 545, 568
BlsI GCNGC 8 cut(s) 246, 266, 330, 340, 401, 543, 546, 569
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 3 cut(s) 7, 25, 234
BmeRI GACNNNNNGTC 1 cut(s) 232
BmgT120I GGNCC 3 cut(s) 7, 25, 234
BmiI GGNNCC 5 cut(s) 8, 26, 185, 236, 248
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 120, 372
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BpmI CTGGAG 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 29
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 2 cut(s) 73, 247
BsaI GGTCTC 2 cut(s) 48, 225
BsaJI CCNNGG 1 cut(s) 568
BsaWI WCCGGW 1 cut(s) 180
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 216, 243, 503
Bse118I RCCGGY 2 cut(s) 180, 240
Bse1I ACTGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 568
BseLI CCNNNNNNNGG 4 cut(s) 46, 216, 243, 503
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 1 cut(s) 497
BseX3I CGGCCG 1 cut(s) 584
BseXI GCAGC 3 cut(s) 276, 411, 556
Bsh1236I CGCG 5 cut(s) 93, 105, 148, 169, 570
Bsh1285I CGRYCG 4 cut(s) 91, 97, 181, 587
BshFI GGCC 1 cut(s) 586
BshNI GGYRCC 2 cut(s) 183, 246
BshTI ACCGGT 1 cut(s) 180
BsiEI CGRYCG 4 cut(s) 91, 97, 181, 587
BsiSI CCGG 3 cut(s) 28, 181, 241
BslFI GGGAC 4 cut(s) 20, 38, 62, 220
BslI CCNNNNNNNGG 4 cut(s) 46, 216, 243, 503
BsmAI GTCTC 5 cut(s) 12, 48, 78, 225, 544
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 4 cut(s) 20, 38, 62, 220
BsnI GGCC 1 cut(s) 586
Bso31I GGTCTC 2 cut(s) 48, 225
Bsp119I TTCGAA 1 cut(s) 347
Bsp143I GATC 1 cut(s) 31
BspANI GGCC 1 cut(s) 586
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 5 cut(s) 93, 105, 148, 169, 570
BspLI GGNNCC 5 cut(s) 8, 26, 185, 236, 248
BspPI GGATC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 347
BspT107I GGYRCC 2 cut(s) 183, 246
BspTNI GGTCTC 2 cut(s) 48, 225
BsrBI CCGCTC 3 cut(s) 159, 189, 328
BsrFI RCCGGY 2 cut(s) 180, 240
BsrI ACTGG 1 cut(s) 497
BssAI RCCGGY 2 cut(s) 180, 240
BssECI CCNNGG 1 cut(s) 568
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 2 cut(s) 73, 247
Bst4CI ACNGT 4 cut(s) 59, 98, 310, 555
BstACI GRCGYC 2 cut(s) 73, 247
BstBAI YACGTR 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 6 cut(s) 173, 251, 287, 293, 505, 594
BstDEI CTNAG 2 cut(s) 384, 537
BstDSI CCRYGG 1 cut(s) 568
BstFNI CGCG 5 cut(s) 93, 105, 148, 169, 570
BstH2I RGCGCY 3 cut(s) 250, 292, 298
BstHHI GCGC 7 cut(s) 150, 171, 249, 291, 297, 509, 603
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 5 cut(s) 12, 48, 78, 225, 544
BstMBI GATC 1 cut(s) 31
BstMCI CGRYCG 4 cut(s) 91, 97, 181, 587
BstMWI GCNNNNNNNGC 4 cut(s) 156, 250, 286, 598
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 5 cut(s) 93, 105, 148, 169, 570
BstV1I GCAGC 3 cut(s) 276, 411, 556
BstZI CGGCCG 1 cut(s) 584
BsuRI GGCC 1 cut(s) 586
BtgI CCRYGG 1 cut(s) 568
BtgZI GCGATG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 6 cut(s) 173, 251, 287, 293, 505, 594
CfoI GCGC 7 cut(s) 150, 171, 249, 291, 297, 509, 603
Cfr10I RCCGGY 2 cut(s) 180, 240
Cfr13I GGNCC 3 cut(s) 7, 25, 234
Cfr42I CCGCGG 1 cut(s) 571
CseI GACGC 2 cut(s) 81, 94
Csp6I GTAC 2 cut(s) 126, 184
CspAI ACCGGT 1 cut(s) 180
CviJI RGCY 6 cut(s) 267, 280, 394, 496, 547, 586
CviKI_1 RGCY 6 cut(s) 267, 280, 394, 496, 547, 586
CviQI GTAC 2 cut(s) 126, 184
DdeI CTNAG 2 cut(s) 384, 537
DinI GGCGCC 1 cut(s) 248
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
DriI GACNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 584
EagI CGGCCG 1 cut(s) 584
Eam1105I GACNNNNNGTC 1 cut(s) 232
EclXI CGGCCG 1 cut(s) 584
Eco31I GGTCTC 2 cut(s) 48, 225
Eco47I GGWCC 3 cut(s) 7, 25, 234
Eco52I CGGCCG 1 cut(s) 584
EcoO109I RGGNCCY 1 cut(s) 234
EcoRI GAATTC 1 cut(s) 42
EgeI GGCGCC 1 cut(s) 248
EheI GGCGCC 1 cut(s) 248
Esp3I CGTCTC 1 cut(s) 78
FaiI YATR 4 cut(s) 378, 425, 558, 560
FaqI GGGAC 4 cut(s) 20, 38, 62, 220
FauI CCCGC 3 cut(s) 164, 200, 496
FblI GTMKAC 1 cut(s) 305
Fnu4HI GCNGC 8 cut(s) 245, 265, 329, 339, 400, 542, 545, 568
Fsp4HI GCNGC 8 cut(s) 245, 265, 329, 339, 400, 542, 545, 568
FspBI CTAG 1 cut(s) 610
GlaI GCGC 7 cut(s) 149, 170, 248, 290, 296, 508, 602
GluI GCNGC 8 cut(s) 245, 265, 329, 339, 400, 542, 545, 568
GsuI CTGGAG 1 cut(s) 207
HaeII RGCGCY 3 cut(s) 250, 292, 298
HaeIII GGCC 1 cut(s) 586
HapII CCGG 3 cut(s) 28, 181, 241
HgaI GACGC 2 cut(s) 81, 94
HhaI GCGC 7 cut(s) 150, 171, 249, 291, 297, 509, 603
Hin1I GRCGYC 2 cut(s) 73, 247
Hin6I GCGC 7 cut(s) 148, 169, 247, 289, 295, 507, 601
HinP1I GCGC 7 cut(s) 148, 169, 247, 289, 295, 507, 601
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 4 cut(s) 302, 463, 479, 562
HpaII CCGG 3 cut(s) 28, 181, 241
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 3 cut(s) 122, 128, 306
Hpy188I TCNGA 1 cut(s) 468
Hpy188III TCNNGA 5 cut(s) 35, 47, 115, 224, 367
Hpy8I GTNNAC 3 cut(s) 122, 128, 306
HpyAV CCTTC 2 cut(s) 328, 364
HpyCH4III ACNGT 4 cut(s) 59, 98, 310, 555
HpyCH4IV ACGT 1 cut(s) 124
HpyF10VI GCNNNNNNNGC 4 cut(s) 156, 250, 286, 598
HpyF3I CTNAG 2 cut(s) 384, 537
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 2 cut(s) 73, 247
HspAI GCGC 7 cut(s) 148, 169, 247, 289, 295, 507, 601
KasI GGCGCC 1 cut(s) 246
KpnI GGTACC 1 cut(s) 187
KspI CCGCGG 1 cut(s) 571
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 5 cut(s) 164, 194, 206, 325, 373
Lsp1109I GCAGC 3 cut(s) 276, 411, 556
LweI GCATC 2 cut(s) 120, 372
MaeI CTAG 1 cut(s) 610
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 4 cut(s) 35, 59, 98, 532
MalI GATC 1 cut(s) 33
MbiI CCGCTC 3 cut(s) 159, 189, 328
MboI GATC 1 cut(s) 31
MboII GAAGA 1 cut(s) 463
MluCI AATT 3 cut(s) 42, 438, 455
Mly113I GGCGCC 1 cut(s) 247
MlyI GAGTC 2 cut(s) 311, 473
MnlI CCTC 6 cut(s) 280, 307, 315, 352, 412, 424
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 570
MspI CCGG 3 cut(s) 28, 181, 241
MspR9I CCNGG 1 cut(s) 29
MvnI CGCG 5 cut(s) 93, 105, 148, 169, 570
MwoI GCNNNNNNNGC 4 cut(s) 156, 250, 286, 598
NarI GGCGCC 1 cut(s) 247
NciI CCSGG 1 cut(s) 29
NdeII GATC 1 cut(s) 31
NlaIV GGNNCC 5 cut(s) 8, 26, 185, 236, 248
NmeAIII GCCGAG 3 cut(s) 101, 420, 573
NmuCI GTSAC 4 cut(s) 35, 59, 98, 532
NspV TTCGAA 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PfeI GAWTC 2 cut(s) 463, 562
PflFI GACNNNGTC 1 cut(s) 104
PinAI ACCGGT 1 cut(s) 180
PkrI GCNGC 8 cut(s) 246, 266, 330, 340, 401, 543, 546, 569
PleI GAGTC 2 cut(s) 310, 473
PluTI GGCGCC 1 cut(s) 250
PpsI GAGTC 2 cut(s) 310, 473
Ppu21I YACGTR 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
PspN4I GGNNCC 5 cut(s) 8, 26, 185, 236, 248
PspPI GGNCC 3 cut(s) 7, 25, 234
PspPPI RGGWCCY 1 cut(s) 234
PsyI GACNNNGTC 1 cut(s) 104
RsaI GTAC 2 cut(s) 127, 185
RsaNI GTAC 2 cut(s) 126, 184
SacII CCGCGG 1 cut(s) 571
SalI GTCGAC 1 cut(s) 304
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 8 cut(s) 245, 265, 329, 339, 400, 542, 545, 568
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 3 cut(s) 7, 25, 234
SchI GAGTC 2 cut(s) 311, 473
ScrFI CCNGG 1 cut(s) 29
SetI ASST 3 cut(s) 127, 133, 236
SfaNI GCATC 2 cut(s) 120, 372
SfcI CTRYAG 1 cut(s) 309
SfoI GGCGCC 1 cut(s) 248
Sfr303I CCGCGG 1 cut(s) 571
SfuI TTCGAA 1 cut(s) 347
SgrAI CRCCGGYG 1 cut(s) 240
SgrBI CCGCGG 1 cut(s) 571
SinI GGWCC 3 cut(s) 7, 25, 234
Sse9I AATT 3 cut(s) 42, 438, 455
SspDI GGCGCC 1 cut(s) 246
SspMI CTAG 1 cut(s) 610
StyD4I CCNGG 1 cut(s) 27
TaaI ACNGT 4 cut(s) 59, 98, 310, 555
TaiI ACGT 1 cut(s) 127
TaqI TCGA 5 cut(s) 305, 347, 368, 477, 482
TaqII GACCGA 4 cut(s) 24, 54, 78, 207
TasI AATT 3 cut(s) 42, 438, 455
TauI GCSGC 5 cut(s) 247, 331, 341, 544, 570
TfiI GAWTC 2 cut(s) 463, 562
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 1 cut(s) 504
TseFI GTSAC 4 cut(s) 35, 59, 98, 532
TseI GCWGC 3 cut(s) 264, 399, 544
Tsp45I GTSAC 4 cut(s) 35, 59, 98, 532
TspDTI ATGAA 2 cut(s) 464, 575
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 1 cut(s) 504
Tth111I GACNNNGTC 1 cut(s) 104
VpaK11BI GGWCC 3 cut(s) 7, 25, 234
XapI RAATTY 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 305
XspI CTAG 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.