Rh4CG116600

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010002
Physical Location & Seq
Forward (+)
22355861 .. 22360606
4746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG116600.1

Sequence Viewer

Length: 612 bp
ATGGGAGACCGAGAAAGAGAGCGAGACAGAGAACGAGACCGGGAGAGAATCCGAGACCGGGACCGAGACAGAGACAGACGCCGTGACAGAGACGACCGTGATCGTGACCGTGACCGTGACCGCGACCGTGTCCGGAGCAAGAGGTCACGTACGCCGGAGCGCAGCAGAGCACGCCACACGCGCTCCCGCACGCGCTCGCCGGACTTGTACCACTCACGCTCCCTCTCCCGTTCCCGCACTCCAGAAGACCGCTCCCACCGCCGCCGCCACCACCACCGCAGCCCCTCCCCGGAGTCGCGGAAGCGTGCGCGGCGCGAGGCGACGGTGGAGGAGGAGAAGGACCGGCAGAAGGTGGTGTCAGAATTCGTGGAGGGGATTGCGAAGGAGCAGCAGAAGCAGAGCAGCGGTGGTGGAGAGGGCGGTGCGGACGAGGACGAGGTTGAGATGATGAAGAAGTTGGGGATTCCGGTAGGGTTTGATTCCACGAAGGGGAAGCCGGTGCAGGGGGCCGATGTGAGTGGTGTTAGGGCCGTCACGAAGCGCCAGCCTAGACAGTACATGAACCGCCGCGGCGGATTTAACCGGCCATTGCCTGCAGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

203

Amino Acids

24.23

Weight (kDa)

11.25

Isoelectric Point (pI)

84.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 144 - 199 1.7e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 252
AccII CGCG 7 cut(s) 123, 181, 193, 298, 310, 315, 570
AccIII TCCGGA 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 3 cut(s) 151, 209, 557
AfiI CCNNNNNNNGG 5 cut(s) 58, 289, 349, 489, 503
Alw21I GWGCWC 1 cut(s) 172
Alw26I GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
Aor13HI TCCGGA 1 cut(s) 132
AoxI GGCC 3 cut(s) 507, 528, 584
ApeKI GCWGC 4 cut(s) 162, 279, 388, 402
ApoI RAATTY 1 cut(s) 362
AspLEI GCGC 7 cut(s) 162, 183, 195, 310, 315, 543, 603
AspS9I GGNCC 4 cut(s) 61, 340, 507, 528
AsuC2I CCSGG 3 cut(s) 41, 59, 290
AvaII GGWCC 2 cut(s) 61, 340
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 4 cut(s) 174, 291, 400, 414
BceAI ACGGC 2 cut(s) 66, 515
BcnI CCSGG 3 cut(s) 41, 59, 290
BcoDI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BfaI CTAG 2 cut(s) 549, 610
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 544
BisI GCNGC 9 cut(s) 163, 262, 265, 280, 311, 389, 403, 568, 571
BlsI GCNGC 9 cut(s) 164, 263, 266, 281, 312, 390, 404, 569, 572
Bme1390I CCNGG 3 cut(s) 41, 59, 290
Bme18I GGWCC 2 cut(s) 61, 340
BmgT120I GGNCC 4 cut(s) 61, 340, 507, 528
BmiI GGNNCC 2 cut(s) 62, 508
BmrFI CCNGG 3 cut(s) 41, 59, 290
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 225
BpuMI CCSGG 3 cut(s) 41, 59, 290
BsaAI YACGTR 1 cut(s) 149
BsaHI GRCGYC 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 30, 48
BsaJI CCNNGG 2 cut(s) 288, 568
BsaWI WCCGGW 2 cut(s) 132, 466
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 203, 233
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 289, 349, 489, 503
Bse118I RCCGGY 3 cut(s) 342, 496, 582
Bse3DI GCAATG 1 cut(s) 587
BseAI TCCGGA 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 288, 568
BseLI CCNNNNNNNGG 5 cut(s) 58, 289, 349, 489, 503
BseMI GCAATG 1 cut(s) 587
BseRI GAGGAG 2 cut(s) 344, 347
BseXI GCAGC 4 cut(s) 174, 291, 400, 414
BsgI GTGCAG 1 cut(s) 521
Bsh1236I CGCG 7 cut(s) 123, 181, 193, 298, 310, 315, 570
Bsh1285I CGRYCG 2 cut(s) 97, 127
BshFI GGCC 3 cut(s) 509, 530, 586
BsiEI CGRYCG 2 cut(s) 97, 127
BsiHKAI GWGCWC 1 cut(s) 172
BsiWI CGTACG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 5 cut(s) 58, 289, 349, 489, 503
BsmAI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 3 cut(s) 509, 530, 586
Bso31I GGTCTC 2 cut(s) 30, 48
Bsp1286I GDGCHC 1 cut(s) 172
Bsp13I TCCGGA 1 cut(s) 132
Bsp143I GATC 1 cut(s) 100
BspANI GGCC 3 cut(s) 509, 530, 586
BspEI TCCGGA 1 cut(s) 132
BspFNI CGCG 7 cut(s) 123, 181, 193, 298, 310, 315, 570
BspLI GGNNCC 2 cut(s) 62, 508
BspMAI CTGCAG 1 cut(s) 598
BspTNI GGTCTC 2 cut(s) 30, 48
BsrBI CCGCTC 1 cut(s) 252
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 3 cut(s) 342, 496, 582
BssAI RCCGGY 3 cut(s) 342, 496, 582
BssECI CCNNGG 2 cut(s) 288, 568
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 1 cut(s) 79
Bst4CI ACNGT 6 cut(s) 98, 110, 116, 128, 325, 555
BstACI GRCGYC 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 6 cut(s) 172, 191, 197, 306, 545, 594
BstDSI CCRYGG 1 cut(s) 568
BstFNI CGCG 7 cut(s) 123, 181, 193, 298, 310, 315, 570
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 7 cut(s) 162, 183, 195, 310, 315, 543, 603
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BstMBI GATC 1 cut(s) 100
BstMCI CGRYCG 2 cut(s) 97, 127
BstMWI GCNNNNNNNGC 6 cut(s) 171, 180, 258, 310, 394, 598
BstSCI CCNGG 3 cut(s) 39, 57, 288
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 7 cut(s) 123, 181, 193, 298, 310, 315, 570
BstV1I GCAGC 4 cut(s) 174, 291, 400, 414
BstV2I GAAGAC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 509, 530, 586
BtgI CCRYGG 1 cut(s) 568
Cac8I GCNNGC 6 cut(s) 172, 191, 197, 306, 545, 594
CfoI GCGC 7 cut(s) 162, 183, 195, 310, 315, 543, 603
Cfr10I RCCGGY 3 cut(s) 342, 496, 582
Cfr13I GGNCC 4 cut(s) 61, 340, 507, 528
Cfr42I CCGCGG 1 cut(s) 571
CseI GACGC 1 cut(s) 87
Csp6I GTAC 3 cut(s) 150, 208, 556
CviAII CATG 1 cut(s) 559
CviJI RGCY 6 cut(s) 282, 496, 509, 530, 547, 586
CviKI_1 RGCY 6 cut(s) 282, 496, 509, 530, 547, 586
CviQI GTAC 3 cut(s) 150, 208, 556
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 584
EciI GGCGGA 1 cut(s) 588
Eco31I GGTCTC 2 cut(s) 30, 48
Eco47I GGWCC 2 cut(s) 61, 340
EcoRI GAATTC 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 1 cut(s) 562
FaiI YATR 1 cut(s) 560
FaqI GGGAC 1 cut(s) 74
FatI CATG 1 cut(s) 558
FauI CCCGC 2 cut(s) 194, 242
Fnu4HI GCNGC 9 cut(s) 163, 262, 265, 280, 311, 389, 403, 568, 571
Fsp4HI GCNGC 9 cut(s) 163, 262, 265, 280, 311, 389, 403, 568, 571
FspBI CTAG 2 cut(s) 549, 610
GlaI GCGC 7 cut(s) 161, 182, 194, 309, 314, 542, 602
GluI GCNGC 9 cut(s) 163, 262, 265, 280, 311, 389, 403, 568, 571
GsuI CTGGAG 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 3 cut(s) 509, 530, 586
HgaI GACGC 1 cut(s) 87
HhaI GCGC 7 cut(s) 162, 183, 195, 310, 315, 543, 603
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 1 cut(s) 562
Hin6I GCGC 7 cut(s) 160, 181, 193, 308, 313, 541, 601
HinP1I GCGC 7 cut(s) 160, 181, 193, 308, 313, 541, 601
HinfI GANTC 4 cut(s) 48, 293, 463, 479
Hpy188I TCNGA 2 cut(s) 53, 361
Hpy188III TCNNGA 4 cut(s) 104, 133, 242, 535
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 4 cut(s) 331, 343, 376, 481
HpyCH4III ACNGT 6 cut(s) 98, 110, 116, 128, 325, 555
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 502, 596
HpyF10VI GCNNNNNNNGC 6 cut(s) 171, 180, 258, 310, 394, 598
HpySE526I ACGT 1 cut(s) 148
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 1 cut(s) 562
HspAI GCGC 7 cut(s) 160, 181, 193, 308, 313, 541, 601
Kpn2I TCCGGA 1 cut(s) 132
KspI CCGCGG 1 cut(s) 571
Kzo9I GATC 1 cut(s) 100
LmnI GCTCC 6 cut(s) 135, 157, 188, 224, 257, 385
Lsp1109I GCAGC 4 cut(s) 174, 291, 400, 414
MaeI CTAG 2 cut(s) 549, 610
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 6 cut(s) 83, 104, 110, 116, 144, 532
MalI GATC 1 cut(s) 102
MbiI CCGCTC 1 cut(s) 252
MboI GATC 1 cut(s) 100
MboII GAAGA 2 cut(s) 257, 463
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 1 cut(s) 362
MlyI GAGTC 1 cut(s) 302
MroI TCCGGA 1 cut(s) 132
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 2 cut(s) 405, 570
MspR9I CCNGG 3 cut(s) 41, 59, 290
MteI GCGCNGCGC 1 cut(s) 311
MvnI CGCG 7 cut(s) 123, 181, 193, 298, 310, 315, 570
MwoI GCNNNNNNNGC 6 cut(s) 171, 180, 258, 310, 394, 598
NciI CCSGG 3 cut(s) 41, 59, 290
NdeII GATC 1 cut(s) 100
NlaIII CATG 1 cut(s) 562
NlaIV GGNNCC 2 cut(s) 62, 508
NmuCI GTSAC 6 cut(s) 83, 104, 110, 116, 144, 532
PfeI GAWTC 3 cut(s) 48, 463, 479
Pfl23II CGTACG 1 cut(s) 149
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 9 cut(s) 164, 263, 266, 281, 312, 390, 404, 569, 572
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 149
PspLI CGTACG 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 62, 508
PspPI GGNCC 4 cut(s) 61, 340, 507, 528
PstI CTGCAG 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 3 cut(s) 151, 209, 557
RsaNI GTAC 3 cut(s) 150, 208, 556
SacII CCGCGG 1 cut(s) 571
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 9 cut(s) 163, 262, 265, 280, 311, 389, 403, 568, 571
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 4 cut(s) 61, 340, 507, 528
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 3 cut(s) 41, 59, 290
SduI GDGCHC 1 cut(s) 172
SetI ASST 4 cut(s) 146, 151, 354, 441
SfcI CTRYAG 1 cut(s) 594
Sfr303I CCGCGG 1 cut(s) 571
SgrBI CCGCGG 1 cut(s) 571
SinI GGWCC 2 cut(s) 61, 340
Sse9I AATT 1 cut(s) 362
SspMI CTAG 2 cut(s) 549, 610
StyD4I CCNGG 3 cut(s) 39, 57, 288
TaaI ACNGT 6 cut(s) 98, 110, 116, 128, 325, 555
TaiI ACGT 1 cut(s) 151
TaqII GACCGA 2 cut(s) 24, 78
TasI AATT 1 cut(s) 362
TatI WGTACW 1 cut(s) 555
TauI GCSGC 5 cut(s) 264, 267, 313, 570, 573
TfiI GAWTC 3 cut(s) 48, 463, 479
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TseFI GTSAC 6 cut(s) 83, 104, 110, 116, 144, 532
TseI GCWGC 4 cut(s) 162, 279, 388, 402
Tsp45I GTSAC 6 cut(s) 83, 104, 110, 116, 144, 532
TspDTI ATGAA 2 cut(s) 464, 575
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 61, 340
XapI RAATTY 1 cut(s) 362
XspI CTAG 2 cut(s) 549, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.