RchiOBHm_Chr4g0401721

Protein of unknown function (DUF1777)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010002
Physical Location & Seq
Forward (+)
19951591 .. 19956333
4743 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37362

Sequence Viewer

Length: 600 bp
ATGGGAGACCGAGAGAGAGAGCGAGACAGAGAACGAGACCGGGAGAGAATCCGAGACCGGGATCGAGACAGAGACAGACGCCGTGACAGAGACGACCGTGATCGTGACCGCGACCGTGTCCGGAGCAAGAGGTCACGCACGCCGGAGCGCAGCAGAGCACGCCACACGCGCTCCCGCACGCGCTCGCCGGACTTGTACCACTCACGCTCCCTCTCCCGTTCCCGCACTCCAGAAGACCGCTCCCACCGCCGCCGCCACCACCACCGCAGCCCCTCCCCGGAGTCGCGGAAGCGTGCGCGGCGCGAGCCGACGGTGGAGGAGGAGAAGGACCGGCAGAAGGTGGTGTCGGAATTCGTGGAGGGGATTGCGAAGGAGCAGCAGAAGCAGAGCAGCGGTGGTGGAGAGGGCGGTGCGGACGAGGACGAGGTTGAGATGATGAAGAAGTTGGGGATTCCGGTAGGGTTTGATTCCACGAAGGGGAAGCCGGTGCAGGGGGCCGATGTGAGTGGTGTTAGGGCCGTCACGAAGCGCCAGCCTAGACAGTACATGAACCGCCGCGGCGGATTTAACCGGCCATTGCCTGCAGAGCGCAATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DUF1777

Protein Analysis

199

Amino Acids

23.71

Weight (kDa)

11.27

Isoelectric Point (pI)

88.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNRNP27 PF08648 140 - 195 1.6e-22 U4/U6.U5 small nuclear ribonucleoproteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 240
AccII CGCG 7 cut(s) 111, 169, 181, 286, 298, 303, 558
AccIII TCCGGA 1 cut(s) 120
AclWI GGATC 1 cut(s) 69
AcoI YGGCCR 1 cut(s) 572
AcsI RAATTY 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 79
AfaI GTAC 2 cut(s) 197, 545
AfiI CCNNNNNNNGG 5 cut(s) 58, 277, 337, 477, 491
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
AlwI GGATC 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 120
AoxI GGCC 3 cut(s) 495, 516, 572
ApeKI GCWGC 4 cut(s) 150, 267, 376, 390
ApoI RAATTY 1 cut(s) 350
AspLEI GCGC 7 cut(s) 150, 171, 183, 298, 303, 531, 591
AspS9I GGNCC 3 cut(s) 328, 495, 516
AsuC2I CCSGG 3 cut(s) 41, 59, 278
AvaII GGWCC 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 4 cut(s) 162, 279, 388, 402
BceAI ACGGC 2 cut(s) 66, 503
BcnI CCSGG 3 cut(s) 41, 59, 278
BcoDI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BfaI CTAG 2 cut(s) 537, 598
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 532
BisI GCNGC 9 cut(s) 151, 250, 253, 268, 299, 377, 391, 556, 559
BlsI GCNGC 9 cut(s) 152, 251, 254, 269, 300, 378, 392, 557, 560
Bme1390I CCNGG 3 cut(s) 41, 59, 278
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 3 cut(s) 328, 495, 516
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 3 cut(s) 41, 59, 278
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 213
BpuMI CCSGG 3 cut(s) 41, 59, 278
BsaHI GRCGYC 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 30, 48
BsaJI CCNNGG 2 cut(s) 276, 556
BsaWI WCCGGW 2 cut(s) 120, 454
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 191, 221
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 277, 337, 477, 491
Bse118I RCCGGY 3 cut(s) 330, 484, 570
Bse3DI GCAATG 1 cut(s) 575
BseAI TCCGGA 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 276, 556
BseLI CCNNNNNNNGG 5 cut(s) 58, 277, 337, 477, 491
BseMI GCAATG 1 cut(s) 575
BseRI GAGGAG 2 cut(s) 332, 335
BseXI GCAGC 4 cut(s) 162, 279, 388, 402
BsgI GTGCAG 1 cut(s) 509
Bsh1236I CGCG 7 cut(s) 111, 169, 181, 286, 298, 303, 558
Bsh1285I CGRYCG 2 cut(s) 97, 115
BshFI GGCC 3 cut(s) 497, 518, 574
BsiEI CGRYCG 2 cut(s) 97, 115
BsiHKAI GWGCWC 1 cut(s) 160
BslI CCNNNNNNNGG 5 cut(s) 58, 277, 337, 477, 491
BsmAI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BsmBI CGTCTC 1 cut(s) 84
BsnI GGCC 3 cut(s) 497, 518, 574
Bso31I GGTCTC 2 cut(s) 30, 48
Bsp1286I GDGCHC 1 cut(s) 160
Bsp13I TCCGGA 1 cut(s) 120
Bsp143I GATC 2 cut(s) 61, 100
BspANI GGCC 3 cut(s) 497, 518, 574
BspEI TCCGGA 1 cut(s) 120
BspFNI CGCG 7 cut(s) 111, 169, 181, 286, 298, 303, 558
BspLI GGNNCC 1 cut(s) 496
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 1 cut(s) 69
BspTNI GGTCTC 2 cut(s) 30, 48
BsrBI CCGCTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 575
BsrFI RCCGGY 3 cut(s) 330, 484, 570
BssAI RCCGGY 3 cut(s) 330, 484, 570
BssECI CCNNGG 2 cut(s) 276, 556
BssMI GATC 2 cut(s) 61, 100
BssNI GRCGYC 1 cut(s) 79
Bst4CI ACNGT 4 cut(s) 98, 116, 313, 543
BstACI GRCGYC 1 cut(s) 79
BstC8I GCNNGC 8 cut(s) 140, 160, 179, 185, 294, 305, 533, 582
BstDSI CCRYGG 1 cut(s) 556
BstFNI CGCG 7 cut(s) 111, 169, 181, 286, 298, 303, 558
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 7 cut(s) 150, 171, 183, 298, 303, 531, 591
BstKTI GATC 2 cut(s) 64, 103
BstMAI GTCTC 6 cut(s) 18, 30, 48, 60, 66, 84
BstMBI GATC 2 cut(s) 61, 100
BstMCI CGRYCG 2 cut(s) 97, 115
BstMWI GCNNNNNNNGC 7 cut(s) 159, 168, 246, 298, 304, 382, 586
BstSCI CCNGG 3 cut(s) 39, 57, 276
BstSFI CTRYAG 1 cut(s) 582
BstUI CGCG 7 cut(s) 111, 169, 181, 286, 298, 303, 558
BstV1I GCAGC 4 cut(s) 162, 279, 388, 402
BstV2I GAAGAC 1 cut(s) 240
BsuRI GGCC 3 cut(s) 497, 518, 574
BtgI CCRYGG 1 cut(s) 556
Cac8I GCNNGC 8 cut(s) 140, 160, 179, 185, 294, 305, 533, 582
CfoI GCGC 7 cut(s) 150, 171, 183, 298, 303, 531, 591
Cfr10I RCCGGY 3 cut(s) 330, 484, 570
Cfr13I GGNCC 3 cut(s) 328, 495, 516
Cfr42I CCGCGG 1 cut(s) 559
CseI GACGC 1 cut(s) 87
Csp6I GTAC 2 cut(s) 196, 544
CviAII CATG 1 cut(s) 547
CviJI RGCY 7 cut(s) 270, 307, 484, 497, 518, 535, 574
CviKI_1 RGCY 7 cut(s) 270, 307, 484, 497, 518, 535, 574
CviQI GTAC 2 cut(s) 196, 544
DpnI GATC 2 cut(s) 63, 102
DpnII GATC 2 cut(s) 61, 100
EaeI YGGCCR 1 cut(s) 572
EciI GGCGGA 1 cut(s) 576
Eco31I GGTCTC 2 cut(s) 30, 48
Eco47I GGWCC 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 350
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 1 cut(s) 550
FaiI YATR 1 cut(s) 548
FatI CATG 1 cut(s) 546
FauI CCCGC 2 cut(s) 182, 230
Fnu4HI GCNGC 9 cut(s) 151, 250, 253, 268, 299, 377, 391, 556, 559
Fsp4HI GCNGC 9 cut(s) 151, 250, 253, 268, 299, 377, 391, 556, 559
FspBI CTAG 2 cut(s) 537, 598
GlaI GCGC 7 cut(s) 149, 170, 182, 297, 302, 530, 590
GluI GCNGC 9 cut(s) 151, 250, 253, 268, 299, 377, 391, 556, 559
GsuI CTGGAG 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 3 cut(s) 497, 518, 574
HgaI GACGC 1 cut(s) 87
HhaI GCGC 7 cut(s) 150, 171, 183, 298, 303, 531, 591
Hin1I GRCGYC 1 cut(s) 79
Hin1II CATG 1 cut(s) 550
Hin6I GCGC 7 cut(s) 148, 169, 181, 296, 301, 529, 589
HinP1I GCGC 7 cut(s) 148, 169, 181, 296, 301, 529, 589
HinfI GANTC 4 cut(s) 48, 281, 451, 467
Hpy188I TCNGA 2 cut(s) 53, 349
Hpy188III TCNNGA 5 cut(s) 65, 104, 121, 230, 523
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 4 cut(s) 319, 331, 364, 469
HpyCH4III ACNGT 4 cut(s) 98, 116, 313, 543
HpyCH4V TGCA 2 cut(s) 490, 584
HpyF10VI GCNNNNNNNGC 7 cut(s) 159, 168, 246, 298, 304, 382, 586
Hsp92I GRCGYC 1 cut(s) 79
Hsp92II CATG 1 cut(s) 550
HspAI GCGC 7 cut(s) 148, 169, 181, 296, 301, 529, 589
Kpn2I TCCGGA 1 cut(s) 120
KspI CCGCGG 1 cut(s) 559
Kzo9I GATC 2 cut(s) 61, 100
LmnI GCTCC 6 cut(s) 123, 145, 176, 212, 245, 373
Lsp1109I GCAGC 4 cut(s) 162, 279, 388, 402
MaeI CTAG 2 cut(s) 537, 598
MaeIII GTNAC 4 cut(s) 83, 104, 132, 520
MalI GATC 2 cut(s) 63, 102
MbiI CCGCTC 1 cut(s) 240
MboI GATC 2 cut(s) 61, 100
MboII GAAGA 2 cut(s) 245, 451
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 1 cut(s) 350
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 9 cut(s) 123, 221, 283, 310, 313, 352, 397, 412, 418
MroI TCCGGA 1 cut(s) 120
MseI TTAA 1 cut(s) 567
MspA1I CMGCKG 2 cut(s) 393, 558
MspR9I CCNGG 3 cut(s) 41, 59, 278
MteI GCGCNGCGC 1 cut(s) 299
MvnI CGCG 7 cut(s) 111, 169, 181, 286, 298, 303, 558
MwoI GCNNNNNNNGC 7 cut(s) 159, 168, 246, 298, 304, 382, 586
NciI CCSGG 3 cut(s) 41, 59, 278
NdeII GATC 2 cut(s) 61, 100
NlaIII CATG 1 cut(s) 550
NlaIV GGNNCC 1 cut(s) 496
NmuCI GTSAC 4 cut(s) 83, 104, 132, 520
PfeI GAWTC 3 cut(s) 48, 451, 467
PflFI GACNNNGTC 1 cut(s) 116
PkrI GCNGC 9 cut(s) 152, 251, 254, 269, 300, 378, 392, 557, 560
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 3 cut(s) 328, 495, 516
PstI CTGCAG 1 cut(s) 586
PsyI GACNNNGTC 1 cut(s) 116
RsaI GTAC 2 cut(s) 197, 545
RsaNI GTAC 2 cut(s) 196, 544
SacII CCGCGG 1 cut(s) 559
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 9 cut(s) 151, 250, 253, 268, 299, 377, 391, 556, 559
Sau3AI GATC 2 cut(s) 61, 100
Sau96I GGNCC 3 cut(s) 328, 495, 516
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 41, 59, 278
SduI GDGCHC 1 cut(s) 160
SetI ASST 3 cut(s) 134, 342, 429
SfcI CTRYAG 1 cut(s) 582
Sfr303I CCGCGG 1 cut(s) 559
SgrBI CCGCGG 1 cut(s) 559
SinI GGWCC 1 cut(s) 328
Sse9I AATT 1 cut(s) 350
SspMI CTAG 2 cut(s) 537, 598
StyD4I CCNGG 3 cut(s) 39, 57, 276
TaaI ACNGT 4 cut(s) 98, 116, 313, 543
TaqI TCGA 1 cut(s) 64
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 350
TatI WGTACW 1 cut(s) 543
TauI GCSGC 5 cut(s) 252, 255, 301, 558, 561
TfiI GAWTC 3 cut(s) 48, 451, 467
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TseFI GTSAC 4 cut(s) 83, 104, 132, 520
TseI GCWGC 4 cut(s) 150, 267, 376, 390
Tsp45I GTSAC 4 cut(s) 83, 104, 132, 520
TspDTI ATGAA 2 cut(s) 452, 563
Tth111I GACNNNGTC 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 1 cut(s) 350
XspI CTAG 2 cut(s) 537, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.