AT5G64230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010139
Physical Location & Seq
Forward (+)
25692425 .. 25694137
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64230.1

Sequence Viewer

Length: 1140 bp
ATGGAGAAGCTGTCATCAGCTGCTGTAGTTCCTCAGCTCCTCCGCAACATCATCGTCGCCGTCGTTGTTTTCGCTGATGAATCTCTCCTTCAAATCTCCGGGAATTCAAAGCTCCTCGAGAAACTCCGTGTATTTCTCGTCACGTGTTTCCTCTTCTTCTTACGCTCTCTTCCTTCAATCGTCTCTTTCGCAAACCCTAATTCGTCAGTCGTCTCTTTCGCCAACCCTTATTCATCGAAGACGAAGAAGAAGAATAAGATACTGGTGATCAACCATTGCGAAGAATCGGGAATCGGTCGAGCAATTTGGCAGTTATTGTCAGCGATGAATGAAATTCCGGTGAGTTCTAGAAAGTACCAAGTGGTTCGATCGTTGGCGGAGAGACTAATCAACGATAATCAAGGAGAAAACTCAGTCGCCTTGTTGGATTTGAACCGTAGGGTTTTAAACGCGTCGTTTCGTACAACTCTAAGCCGGTTAGAGACGGCGGTTGAGCGTAACCCTAACCGGAGAGATATAGATGAACCGGTTCGTCGTGGATTAAACCGGGTTGTCAGAGCTGTGGTTAGAGCCGTGGGAGATGGGTTTATCGGTTGGGGTGGAGAAGAGACGGCGGATCAGACGGCGGAAACGTCGGAGAAACTGGCGGCGGAATTGCTTTGGTTGGCGGAGAAGATGGCGGTTTATGGATTCGTGGATGAAGCCGTTGAGAAATGGGCTTCGGCTTCTAACTTAGCTTGGCTTGCTCTTTCGTGTGAGCCACGTCTTCAATGCTCTTTGATCCAAATCTCTGCCTTACTATTCAAAGAAGCCAAGGACATTAAAAAGGGAAGCGAAGAAGAAGAAGGAGAGGAAGCAAAATTGAGAGAGATAAAGAAGAAGATGCTTATTTCGTGGATTCCGTTGCTGTGCCGAGCCAGCAACGGAGCTGATAAACCGGTGTTAAGAAGTGCGGAACGAGCGTATCTAGAGAAAGTGTTGGAGAAGATGATATCTGAACTAAAAGAAGAGGAGCAAGAACGAGTTCTCTCACTCTGGCTTCACCATTACACACATTGCGCTTCCTCTGACTGGCCTGACCTCAACGGCTCTTATGTTCGTTGGTGCCATTCTTCTCGCCAGCTTTTGCTCTTAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

42.67

Weight (kDa)

8.38

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1104
AccII CGCG 1 cut(s) 452
AclWI GGATC 2 cut(s) 624, 775
AcsI RAATTY 2 cut(s) 103, 333
AcvI CACGTG 1 cut(s) 144
AfaI GTAC 2 cut(s) 356, 463
AfiI CCNNNNNNNGG 1 cut(s) 1071
AflIII ACRYGT 2 cut(s) 143, 450
AgeI ACCGGT 2 cut(s) 526, 937
AgsI TTSAA 6 cut(s) 92, 108, 177, 433, 770, 805
AjiI CACGTC 1 cut(s) 764
AluBI AGCT 8 cut(s) 10, 20, 37, 112, 560, 737, 929, 1123
AluI AGCT 8 cut(s) 10, 20, 37, 112, 560, 737, 929, 1123
Alw26I GTCTC 5 cut(s) 187, 217, 376, 476, 602
AlwI GGATC 2 cut(s) 624, 775
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 1073
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 2 cut(s) 103, 333
AsiGI ACCGGT 2 cut(s) 526, 937
Asp700I GAANNNNTTC 2 cut(s) 528, 1023
AspLEI GCGC 1 cut(s) 1061
AsuC2I CCSGG 2 cut(s) 100, 548
AsuHPI GGTGA 3 cut(s) 277, 352, 1034
AvaI CYCGRG 1 cut(s) 116
BaeI ACNNNNGTAYC 2 cut(s) 947, 980
BanI GGYRCC 1 cut(s) 1104
BbrPI CACGTG 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 245, 758
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 7
BccI CCATC 2 cut(s) 575, 670
BceAI ACGGC 7 cut(s) 44, 501, 557, 627, 639, 689, 1102
BcgI CGANNNNNNTGC 2 cut(s) 34, 68
BclI TGATCA 1 cut(s) 267
BcnI CCSGG 2 cut(s) 100, 548
BcoDI GTCTC 5 cut(s) 187, 217, 376, 476, 602
BfaI CTAG 3 cut(s) 348, 968, 1138
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 2 cut(s) 21, 648
BlsI GCNGC 2 cut(s) 22, 649
Bme1390I CCNGG 2 cut(s) 100, 548
BmeT110I CYCGRG 1 cut(s) 116
BmgBI CACGTC 1 cut(s) 764
BmiI GGNNCC 1 cut(s) 1106
BmrFI CCNGG 2 cut(s) 100, 548
BmsI GCATC 1 cut(s) 873
BpiI GAAGAC 2 cut(s) 245, 758
Bpu10I CCTNAGC 1 cut(s) 33
BpuMI CCSGG 2 cut(s) 100, 548
BsaAI YACGTR 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 785
BsaJI CCNNGG 2 cut(s) 573, 813
BsaWI WCCGGW 4 cut(s) 337, 507, 526, 937
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 1071
Bse118I RCCGGY 3 cut(s) 474, 526, 937
Bse1I ACTGG 3 cut(s) 267, 648, 1076
Bse3DI GCAATG 2 cut(s) 274, 1054
Bse8I GATNNNNATC 1 cut(s) 785
BseDI CCNNGG 2 cut(s) 573, 813
BseGI GGATG 1 cut(s) 703
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 1 cut(s) 1071
BseMI GCAATG 2 cut(s) 274, 1054
BseMII CTCAG 2 cut(s) 47, 426
BseNI ACTGG 3 cut(s) 267, 648, 1076
BseRI GAGGAG 3 cut(s) 29, 104, 1025
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 452
Bsh1285I CGRYCG 2 cut(s) 298, 371
BshFI GGCC 1 cut(s) 1075
BshNI GGYRCC 1 cut(s) 1104
BshTI ACCGGT 2 cut(s) 526, 937
BsiEI CGRYCG 2 cut(s) 298, 371
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 7 cut(s) 99, 338, 475, 508, 527, 547, 938
BslI CCNNNNNNNGG 1 cut(s) 1071
BsmAI GTCTC 5 cut(s) 187, 217, 376, 476, 602
BsmBI CGTCTC 4 cut(s) 187, 217, 476, 602
BsnI GGCC 1 cut(s) 1075
BsoBI CYCGRG 1 cut(s) 116
Bsp143I GATC 4 cut(s) 267, 368, 616, 780
BspANI GGCC 1 cut(s) 1075
BspCNI CTCAG 2 cut(s) 46, 425
BspFNI CGCG 1 cut(s) 452
BspLI GGNNCC 1 cut(s) 1106
BspPI GGATC 2 cut(s) 624, 775
BspT107I GGYRCC 1 cut(s) 1104
BsrDI GCAATG 2 cut(s) 274, 1054
BsrFI RCCGGY 3 cut(s) 474, 526, 937
BsrI ACTGG 3 cut(s) 267, 648, 1076
BssAI RCCGGY 3 cut(s) 474, 526, 937
BssECI CCNNGG 2 cut(s) 573, 813
BssMI GATC 4 cut(s) 267, 368, 616, 780
BssT1I CCWWGG 1 cut(s) 813
Bst4CI ACNGT 1 cut(s) 437
Bst6I CTCTTC 4 cut(s) 158, 174, 600, 1002
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 3 cut(s) 744, 919, 1121
BstDEI CTNAG 5 cut(s) 33, 412, 470, 733, 1132
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 1061
BstKTI GATC 4 cut(s) 270, 371, 619, 783
BstMAI GTCTC 5 cut(s) 187, 217, 376, 476, 602
BstMBI GATC 4 cut(s) 267, 368, 616, 780
BstMCI CGRYCG 2 cut(s) 298, 371
BstMWI GCNNNNNNNGC 3 cut(s) 743, 918, 959
BstSCI CCNGG 2 cut(s) 98, 546
BstSFI CTRYAG 1 cut(s) 24
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 2 cut(s) 245, 758
BsuRI GGCC 1 cut(s) 1075
BtgI CCRYGG 1 cut(s) 573
BtgZI GCGATG 1 cut(s) 338
BtrI CACGTC 1 cut(s) 764
BtsCI GGATG 1 cut(s) 703
Cac8I GCNNGC 3 cut(s) 744, 919, 1121
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 1 cut(s) 1061
Cfr10I RCCGGY 3 cut(s) 474, 526, 937
CseI GACGC 1 cut(s) 441
Csp6I GTAC 2 cut(s) 355, 462
CspAI ACCGGT 2 cut(s) 526, 937
CviQI GTAC 2 cut(s) 355, 462
DdeI CTNAG 5 cut(s) 33, 412, 470, 733, 1132
DpnI GATC 4 cut(s) 269, 370, 618, 782
DpnII GATC 4 cut(s) 267, 368, 616, 780
DraI TTTAAA 1 cut(s) 447
Eam1104I CTCTTC 4 cut(s) 158, 174, 600, 1002
EarI CTCTTC 4 cut(s) 158, 174, 600, 1002
EciI GGCGGA 5 cut(s) 392, 629, 641, 665, 683
Eco130I CCWWGG 1 cut(s) 813
Eco32I GATATC 1 cut(s) 993
Eco72I CACGTG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 103
EcoRV GATATC 1 cut(s) 993
EcoT14I CCWWGG 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 813
Esp3I CGTCTC 4 cut(s) 187, 217, 476, 602
FaiI YATR 3 cut(s) 518, 687, 1095
FbaI TGATCA 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 21, 648
FokI GGATG 1 cut(s) 710
Fsp4HI GCNGC 2 cut(s) 21, 648
FspBI CTAG 3 cut(s) 348, 968, 1138
GlaI GCGC 1 cut(s) 1060
GluI GCNGC 2 cut(s) 21, 648
HaeIII GGCC 1 cut(s) 1075
HapII CCGG 7 cut(s) 99, 338, 475, 508, 527, 547, 938
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 1061
Hin6I GCGC 1 cut(s) 1059
HinP1I GCGC 1 cut(s) 1059
HinfI GANTC 5 cut(s) 80, 284, 291, 690, 898
HpaII CCGG 7 cut(s) 99, 338, 475, 508, 527, 547, 938
HphI GGTGA 3 cut(s) 277, 352, 1034
Hpy188I TCNGA 5 cut(s) 557, 621, 637, 997, 1069
Hpy188III TCNNGA 4 cut(s) 118, 288, 348, 968
Hpy99I CGWCG 5 cut(s) 59, 65, 457, 537, 637
HpyAV CCTTC 3 cut(s) 98, 183, 839
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 3 cut(s) 143, 632, 763
HpyF10VI GCNNNNNNNGC 3 cut(s) 743, 918, 959
HpyF3I CTNAG 5 cut(s) 33, 412, 470, 733, 1132
HpySE526I ACGT 3 cut(s) 143, 632, 763
HspAI GCGC 1 cut(s) 1059
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 4 cut(s) 267, 368, 616, 780
LmnI GCTCC 4 cut(s) 42, 117, 926, 1012
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 873
MaeI CTAG 3 cut(s) 348, 968, 1138
MaeII ACGT 3 cut(s) 143, 632, 763
MaeIII GTNAC 2 cut(s) 139, 497
MalI GATC 4 cut(s) 269, 370, 618, 782
MboI GATC 4 cut(s) 267, 368, 616, 780
MluCI AATT 6 cut(s) 103, 199, 303, 333, 653, 860
MluI ACGCGT 1 cut(s) 450
MmeI TCCRAC 3 cut(s) 405, 615, 960
MnlI CCTC 8 cut(s) 42, 50, 125, 161, 844, 1003, 1075, 1091
MroXI GAANNNNTTC 2 cut(s) 528, 1023
MseI TTAA 4 cut(s) 446, 542, 822, 944
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 7 cut(s) 99, 338, 475, 508, 527, 547, 938
MspR9I CCNGG 2 cut(s) 100, 548
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 3 cut(s) 743, 918, 959
NciI CCSGG 2 cut(s) 100, 548
NdeII GATC 4 cut(s) 267, 368, 616, 780
NlaIV GGNNCC 1 cut(s) 1106
NmeAIII GCCGAG 1 cut(s) 938
NmuCI GTSAC 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 629, 899
PdmI GAANNNNTTC 2 cut(s) 528, 1023
PfeI GAWTC 5 cut(s) 80, 284, 291, 690, 898
PfoI TCCNGGA 1 cut(s) 98
PinAI ACCGGT 2 cut(s) 526, 937
PkrI GCNGC 2 cut(s) 22, 649
Ple19I CGATCG 1 cut(s) 371
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
Ppu21I YACGTR 1 cut(s) 144
PspCI CACGTG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 1106
PstNI CAGNNNCTG 1 cut(s) 23
PvuI CGATCG 1 cut(s) 371
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 356, 463
RsaNI GTAC 2 cut(s) 355, 462
SaqAI TTAA 4 cut(s) 446, 542, 822, 944
SatI GCNGC 2 cut(s) 21, 648
Sau3AI GATC 4 cut(s) 267, 368, 616, 780
ScrFI CCNGG 2 cut(s) 100, 548
SfaNI GCATC 1 cut(s) 873
SfcI CTRYAG 1 cut(s) 24
Sfr274I CTCGAG 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 6 cut(s) 103, 199, 303, 333, 653, 860
SspMI CTAG 3 cut(s) 348, 968, 1138
StyD4I CCNGG 2 cut(s) 98, 546
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 3 cut(s) 146, 635, 766
TaqI TCGA 4 cut(s) 117, 236, 298, 367
TaqII GACCGA 1 cut(s) 284
TasI AATT 6 cut(s) 103, 199, 303, 333, 653, 860
TauI GCSGC 1 cut(s) 650
TfiI GAWTC 5 cut(s) 80, 284, 291, 690, 898
Tru1I TTAA 4 cut(s) 446, 542, 822, 944
Tru9I TTAA 4 cut(s) 446, 542, 822, 944
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 6 cut(s) 93, 222, 341, 345, 537, 714
TspGWI ACGGA 3 cut(s) 116, 891, 939
XapI RAATTY 2 cut(s) 103, 333
XbaI TCTAGA 2 cut(s) 347, 967
XhoI CTCGAG 1 cut(s) 116
XmnI GAANNNNTTC 2 cut(s) 528, 1023
XspI CTAG 3 cut(s) 348, 968, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.