Rorug06G0045800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010139
Physical Location & Seq
Forward (+)
5996310 .. 5996992
683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0045800.1

Sequence Viewer

Length: 384 bp
ATGATGAATGTGGTTCGTTTGAGCAGGCATGCTATAGAACAATACGTTGCAGAAGCAAGAAACATCTTCAATGACATACTTGAAGCCCTTGCTGAAGCCTTATCACTAGAAAGACATGTATTCCTCCAAAACTTTGACCTCTATGAGACTGAGATCAAGGTGAGAATGAACTACTACCCACCATGCCCAAGGCCAGACCAAGCCCTTGGTCTAACTCCACACACTGATGCCAGTGCCCTCACTCTCCTCCTTCAATTCGAAGCTAGCGGAGGGTTCCAAGTGTTGAAGGCCATGGAATGGGTCACGTACTGTGCCTTGGCCAAAAGACGCAATGCTAGTGAATGTGGGAGACCTACTAGAGATTATGAGCAATGGGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

14.65

Weight (kDa)

5.34

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 54 - 103 5e-13 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 297
AciI CCGC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 4 cut(s) 70, 83, 254, 286
AluBI AGCT 1 cut(s) 263
AluI AGCT 1 cut(s) 263
Alw26I GTCTC 2 cut(s) 140, 343
AoxI GGCC 3 cut(s) 191, 288, 318
AsuHPI GGTGA 1 cut(s) 172
AsuII TTCGAA 1 cut(s) 258
AsuNHI GCTAGC 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 238
BalI TGGCCA 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 140, 343
BfaI CTAG 4 cut(s) 107, 264, 336, 357
BfmI CTRYAG 1 cut(s) 33
BmiI GGNNCC 1 cut(s) 275
BmsI GCATC 1 cut(s) 217
BmtI GCTAGC 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 4 cut(s) 188, 205, 291, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 2 cut(s) 337, 377
BseDI CCNNGG 4 cut(s) 188, 205, 291, 315
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 2 cut(s) 337, 377
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 238
BshFI GGCC 3 cut(s) 193, 290, 320
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 140, 343
BsnI GGCC 3 cut(s) 193, 290, 320
Bso31I GGTCTC 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 1 cut(s) 153
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 1 cut(s) 267
BspANI GGCC 3 cut(s) 193, 290, 320
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 275
BspOI GCTAGC 1 cut(s) 267
BspT104I TTCGAA 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 337, 377
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 4 cut(s) 188, 205, 291, 315
BssMI GATC 1 cut(s) 153
BssT1I CCWWGG 4 cut(s) 188, 205, 291, 315
Bst4CI ACNGT 1 cut(s) 311
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 3 cut(s) 26, 30, 265
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 382
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 2 cut(s) 140, 343
BstMBI GATC 1 cut(s) 153
BstNSI RCATGY 2 cut(s) 32, 119
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 206
BsuRI GGCC 3 cut(s) 193, 290, 320
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 222, 238
Cac8I GCNNGC 3 cut(s) 26, 30, 265
CseI GACGC 1 cut(s) 336
Csp6I GTAC 1 cut(s) 307
CviAII CATG 4 cut(s) 29, 116, 183, 292
CviJI RGCY 7 cut(s) 86, 98, 193, 203, 263, 290, 320
CviKI_1 RGCY 7 cut(s) 86, 98, 193, 203, 263, 290, 320
CviQI GTAC 1 cut(s) 307
DdeI CTNAG 1 cut(s) 150
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 318
Eco130I CCWWGG 4 cut(s) 188, 205, 291, 315
Eco31I GGTCTC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 114
EcoT14I CCWWGG 4 cut(s) 188, 205, 291, 315
ErhI CCWWGG 4 cut(s) 188, 205, 291, 315
FaeI CATG 4 cut(s) 32, 119, 186, 295
FaiI YATR 8 cut(s) 30, 35, 77, 117, 144, 184, 293, 366
FatI CATG 4 cut(s) 28, 115, 182, 291
FspBI CTAG 4 cut(s) 107, 264, 336, 357
HaeIII GGCC 3 cut(s) 193, 290, 320
HgaI GACGC 1 cut(s) 336
Hin1II CATG 4 cut(s) 32, 119, 186, 295
HphI GGTGA 1 cut(s) 172
HpyAV CCTTC 2 cut(s) 260, 280
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 2 cut(s) 45, 305
HpyCH4V TGCA 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 2 cut(s) 45, 305
Hsp92II CATG 4 cut(s) 32, 119, 186, 295
Kzo9I GATC 1 cut(s) 153
LpnPI CCDG 3 cut(s) 10, 207, 244
LweI GCATC 1 cut(s) 217
MaeI CTAG 4 cut(s) 107, 264, 336, 357
MaeII ACGT 2 cut(s) 45, 305
MaeIII GTNAC 1 cut(s) 301
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 320
MnlI CCTC 5 cut(s) 134, 149, 248, 257, 263
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MslI CAYNNNNRTG 1 cut(s) 225
Msp20I TGGCCA 1 cut(s) 320
NcoI CCATGG 1 cut(s) 291
NdeII GATC 1 cut(s) 153
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 4 cut(s) 32, 119, 186, 295
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 301
NspI RCATGY 2 cut(s) 32, 119
NspV TTCGAA 1 cut(s) 258
PaeI GCATGC 1 cut(s) 32
PciI ACATGT 1 cut(s) 115
PflMI CCANNNNNTGG 1 cut(s) 297
Ppu21I YACGTR 1 cut(s) 306
PscI ACATGT 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 275
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 225
Sau3AI GATC 1 cut(s) 153
SduI GDGCHC 1 cut(s) 238
SetI ASST 6 cut(s) 48, 141, 162, 265, 308, 355
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 33
SfuI TTCGAA 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 225
SphI GCATGC 1 cut(s) 32
Sse9I AATT 1 cut(s) 254
SsiI CCGC 1 cut(s) 267
SspMI CTAG 4 cut(s) 107, 264, 336, 357
StyI CCWWGG 4 cut(s) 188, 205, 291, 315
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 2 cut(s) 48, 308
TaqI TCGA 1 cut(s) 258
TasI AATT 1 cut(s) 254
TscAI CASTG 2 cut(s) 229, 238
TseFI GTSAC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 20, 182
TspRI CASTG 2 cut(s) 229, 238
Van91I CCANNNNNTGG 1 cut(s) 297
XceI RCATGY 2 cut(s) 32, 119
XspI CTAG 4 cut(s) 107, 264, 336, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.