MD05G1044100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010139
Physical Location & Seq
Reverse (-)
7546696 .. 7558136
11441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1044100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGTGATGATGGAAGGCTGGTCTCCAACTACCATATCTCCTTTGCTTCTCCGCAACCTCATAGCCTCCCTCTTTATCTTCGCAGACAAATCCTTCCTAAACTTGTCTGAGAAATCCAAACTCCTCCAACTCCTTCGCTATACTTTCGTCACTTCCTTCCTCTTCCTCCTCCGTTTTCTTCCTCCTCTAAACCCTAACAATAACCAACCCTACAAGCCTCCCAACAAGAATGCTAGTGATCACACTAGTGATCATTATGATTATACGCTCTCCTCATGCGGCGGCGGTGCTACTATTACTGGCGGCGGCGACTCCGCTATCGCTCGAGCACTTTCGCAGCTCTTGTCCCTCGTCAACGACATCCCCGTAAACTCCAGGAAGTACCAAGTTGTTCGCTCTTTAGCGGAGAACCTCATCGACGACAACCACAAAGAGGGCGTAGAGGCTTTACGTCAAGTCAACCGTACGGTTCTATCCGCGGCTTTTTCCACCACTCTGAGCCAGCTTGAAGCCGCCACCATTCTCGAACAACAAGGAGAAGTGCGTGGGGGTGATGGCGTCGATTTGTTGGGGAACGCTCGGCCGGCGGAGAACCGGTGGAGCCCGGTCCTACGGGTGGTTCGATCGATATGGGACGTGGCGTGGATAAGGGGGACGAGGGCGAGCGGGACGAGGAGCTCGGCGGAGAAACTGGCGGCTGAGGTGCTTTGGTTGGCTGAGAAGCTGGCAGCTTGTGACTTGGCTGATGAAGCAGTGCGGAGATGGGCTGCCTCCTTTAATTTGGCTTGGCTCTCTCTTTCCGTTGAGCCGCGCTTACAAGCATCCTTGGTCAAGGTTTCGGACAACTCAGCTTGGCCTCTTGTAGCAGCAGCCTTGCCCGAATGCAACTTGAGAGGCAGTGACTCATCAGCTTTTTTACTTTACTTTATGGGCTGTATAGCTGGTCAGTCTATACCACTTGGCCTTTGGGTAGGTGAGCAAGTGGTCTTCATGAGCTGCACCAAGTCAGAAAAGCCCTTTTCTGTTTTCTGGGCTTTGGCTGGGCTTAGGCCGATTCTTCCTTTAGGCATCCTTTTGGGCCAGCCCCTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

365

Amino Acids

39.73

Weight (kDa)

7.62

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 666
AccII CGCG 2 cut(s) 479, 811
AcoI YGGCCR 1 cut(s) 581
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 2 cut(s) 383, 466
AfiI CCNNNNNNNGG 2 cut(s) 433, 616
AgeI ACCGGT 1 cut(s) 594
AgsI TTSAA 1 cut(s) 509
AhlI ACTAGT 1 cut(s) 245
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 1 cut(s) 374
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 9 cut(s) 340, 505, 678, 724, 731, 851, 911, 941, 996
AluI AGCT 9 cut(s) 340, 505, 678, 724, 731, 851, 911, 941, 996
Alw21I GWGCWC 2 cut(s) 331, 680
Alw26I GTCTC 1 cut(s) 27
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 5 cut(s) 581, 854, 961, 1049, 1078
ApeKI GCWGC 6 cut(s) 337, 728, 767, 866, 869, 996
AsiGI ACCGGT 1 cut(s) 594
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 2 cut(s) 607, 1078
AsuC2I CCSGG 1 cut(s) 605
AsuHPI GGTGA 3 cut(s) 16, 563, 986
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 1 cut(s) 607
BanII GRGCYC 2 cut(s) 605, 680
BbsI GAAGAC 1 cut(s) 979
Bbv12I GWGCWC 2 cut(s) 331, 680
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 6 cut(s) 349, 740, 754, 878, 881, 983
BccI CCATC 3 cut(s) 4, 548, 756
BciT130I CCWGG 1 cut(s) 376
BclI TGATCA 2 cut(s) 238, 250
BcnI CCSGG 1 cut(s) 605
BcoDI GTCTC 1 cut(s) 27
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 2 cut(s) 234, 246
Bme1390I CCNGG 2 cut(s) 376, 605
Bme18I GGWCC 1 cut(s) 607
BmeT110I CYCGRG 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 2 cut(s) 607, 1078
BmiI GGNNCC 1 cut(s) 602
BmrFI CCNGG 2 cut(s) 376, 605
BmsI GCATC 2 cut(s) 830, 1077
BpiI GAAGAC 1 cut(s) 979
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 2 cut(s) 699, 1046
BpuEI CTTGAG 1 cut(s) 910
BpuMI CCSGG 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 558
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 477, 825
BsaWI WCCGGW 1 cut(s) 594
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 581, 611
Bsc4I CCNNNNNNNGG 2 cut(s) 433, 616
Bse118I RCCGGY 2 cut(s) 583, 594
Bse1I ACTGG 2 cut(s) 304, 696
BseBI CCWGG 1 cut(s) 376
BseCI ATCGAT 1 cut(s) 626
BseDI CCNNGG 2 cut(s) 477, 825
BseGI GGATG 3 cut(s) 360, 821, 1068
BseLI CCNNNNNNNGG 2 cut(s) 433, 616
BseMII CTCAG 5 cut(s) 99, 488, 690, 708, 861
BseNI ACTGG 2 cut(s) 304, 696
BseRI GAGGAG 5 cut(s) 113, 158, 174, 262, 688
BseX3I CGGCCG 1 cut(s) 581
BseXI GCAGC 6 cut(s) 349, 740, 754, 878, 881, 983
BseYI CCCAGC 1 cut(s) 1040
BsgI GTGCAG 1 cut(s) 982
Bsh1236I CGCG 2 cut(s) 479, 811
Bsh1285I CGRYCG 2 cut(s) 584, 626
BshFI GGCC 5 cut(s) 583, 856, 963, 1051, 1080
BshTI ACCGGT 1 cut(s) 594
BshVI ATCGAT 1 cut(s) 626
BsiEI CGRYCG 2 cut(s) 584, 626
BsiHKAI GWGCWC 2 cut(s) 331, 680
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 3 cut(s) 584, 595, 605
BsiWI CGTACG 1 cut(s) 464
BslFI GGGAC 4 cut(s) 331, 647, 667, 682
BslI CCNNNNNNNGG 2 cut(s) 433, 616
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 4 cut(s) 331, 647, 667, 682
BsmI GAATGC 2 cut(s) 235, 887
BsnI GGCC 5 cut(s) 583, 856, 963, 1051, 1080
Bso31I GGTCTC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 3 cut(s) 331, 605, 680
Bsp143I GATC 3 cut(s) 238, 250, 623
BspANI GGCC 5 cut(s) 583, 856, 963, 1051, 1080
BspCNI CTCAG 5 cut(s) 100, 489, 691, 709, 860
BspDI ATCGAT 1 cut(s) 626
BspFNI CGCG 2 cut(s) 479, 811
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 27
BsrBI CCGCTC 1 cut(s) 666
BsrFI RCCGGY 2 cut(s) 583, 594
BsrI ACTGG 2 cut(s) 304, 696
BssAI RCCGGY 2 cut(s) 583, 594
BssECI CCNNGG 2 cut(s) 477, 825
BssMI GATC 3 cut(s) 238, 250, 623
BssNI GRCGYC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 825
Bst2UI CCWGG 1 cut(s) 376
Bst4CI ACNGT 2 cut(s) 464, 469
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 558
BstC8I GCNNGC 5 cut(s) 503, 585, 664, 726, 1082
BstDEI CTNAG 6 cut(s) 108, 497, 699, 717, 847, 1046
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 3 cut(s) 360, 821, 1068
BstFNI CGCG 2 cut(s) 479, 811
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 3 cut(s) 241, 253, 626
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 3 cut(s) 238, 250, 623
BstMCI CGRYCG 2 cut(s) 584, 626
BstMWI GCNNNNNNNGC 2 cut(s) 584, 749
BstNI CCWGG 1 cut(s) 376
BstSCI CCNGG 2 cut(s) 374, 603
BstUI CGCG 2 cut(s) 479, 811
BstV1I GCAGC 6 cut(s) 349, 740, 754, 878, 881, 983
BstV2I GAAGAC 1 cut(s) 979
BstZI CGGCCG 1 cut(s) 581
Bsu15I ATCGAT 1 cut(s) 626
BsuRI GGCC 5 cut(s) 583, 856, 963, 1051, 1080
BsuTUI ATCGAT 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 3 cut(s) 360, 821, 1068
BtsI GCAGTG 2 cut(s) 759, 904
BtsIMutI CAGTG 2 cut(s) 759, 904
Cac8I GCNNGC 5 cut(s) 503, 585, 664, 726, 1082
CciI TCATGA 1 cut(s) 990
CfoI GCGC 1 cut(s) 813
Cfr10I RCCGGY 2 cut(s) 583, 594
Cfr13I GGNCC 2 cut(s) 607, 1078
Cfr42I CCGCGG 1 cut(s) 480
ClaI ATCGAT 1 cut(s) 626
CseI GACGC 1 cut(s) 547
Csp6I GTAC 2 cut(s) 382, 465
CspAI ACCGGT 1 cut(s) 594
CviAII CATG 2 cut(s) 276, 991
CviQI GTAC 2 cut(s) 382, 465
DdeI CTNAG 6 cut(s) 108, 497, 699, 717, 847, 1046
DpnI GATC 3 cut(s) 240, 252, 625
DpnII GATC 3 cut(s) 238, 250, 623
EaeI YGGCCR 1 cut(s) 581
EagI CGGCCG 1 cut(s) 581
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 2 cut(s) 602, 698
Ecl136II GAGCTC 1 cut(s) 678
EclXI CGGCCG 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 825
Eco24I GRGCYC 2 cut(s) 605, 680
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 607
Eco52I CGGCCG 1 cut(s) 581
Eco53kI GAGCTC 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 324
EcoICRI GAGCTC 1 cut(s) 678
EcoRII CCWGG 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 2 cut(s) 605, 680
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 2 cut(s) 279, 994
FaqI GGGAC 4 cut(s) 331, 647, 667, 682
FatI CATG 2 cut(s) 275, 990
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 2 cut(s) 238, 250
FokI GGATG 3 cut(s) 347, 808, 1055
FriOI GRGCYC 2 cut(s) 605, 680
FspBI CTAG 2 cut(s) 234, 246
GlaI GCGC 1 cut(s) 812
GsaI CCCAGC 1 cut(s) 1044
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 5 cut(s) 583, 856, 963, 1051, 1080
HapII CCGG 3 cut(s) 584, 595, 605
HgaI GACGC 1 cut(s) 547
HhaI GCGC 1 cut(s) 813
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 2 cut(s) 279, 994
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HincII GTYRAC 2 cut(s) 355, 460
HindII GTYRAC 2 cut(s) 355, 460
HinfI GANTC 3 cut(s) 311, 902, 1054
HpaII CCGG 3 cut(s) 584, 595, 605
HphI GGTGA 3 cut(s) 16, 563, 986
Hpy166II GTNNAC 3 cut(s) 355, 370, 460
Hpy188I TCNGA 4 cut(s) 109, 498, 841, 1009
Hpy188III TCNNGA 3 cut(s) 524, 991, 1092
Hpy8I GTNNAC 3 cut(s) 355, 370, 460
Hpy99I CGWCG 2 cut(s) 422, 563
HpyAV CCTTC 4 cut(s) 8, 103, 143, 166
HpyCH4III ACNGT 2 cut(s) 464, 469
HpyCH4IV ACGT 2 cut(s) 451, 636
HpyCH4V TGCA 2 cut(s) 885, 999
HpyF10VI GCNNNNNNNGC 2 cut(s) 584, 749
HpyF3I CTNAG 6 cut(s) 108, 497, 699, 717, 847, 1046
HpySE526I ACGT 2 cut(s) 451, 636
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 2 cut(s) 279, 994
HspAI GCGC 1 cut(s) 811
KroI GCCGGC 1 cut(s) 583
KroNI GCCGGC 1 cut(s) 585
Ksp22I TGATCA 2 cut(s) 238, 250
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 3 cut(s) 238, 250, 623
LmnI GCTCC 2 cut(s) 600, 675
Lsp1109I GCAGC 6 cut(s) 349, 740, 754, 878, 881, 983
LweI GCATC 2 cut(s) 830, 1077
MaeI CTAG 2 cut(s) 234, 246
MaeII ACGT 2 cut(s) 451, 636
MaeIII GTNAC 3 cut(s) 148, 734, 899
MalI GATC 3 cut(s) 240, 252, 625
MbiI CCGCTC 1 cut(s) 666
MboI GATC 3 cut(s) 238, 250, 623
MboII GAAGA 5 cut(s) 70, 154, 170, 979, 1049
MhlI GDGCHC 3 cut(s) 331, 605, 680
MluCI AATT 1 cut(s) 778
MlyI GAGTC 2 cut(s) 305, 896
MmeI TCCRAC 2 cut(s) 50, 151
MroNI GCCGGC 1 cut(s) 583
MseI TTAA 1 cut(s) 777
MslI CAYNNNNRTG 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 3 cut(s) 584, 595, 605
MspR9I CCNGG 2 cut(s) 376, 605
Mva1269I GAATGC 2 cut(s) 235, 887
MvaI CCWGG 1 cut(s) 376
MvnI CGCG 2 cut(s) 479, 811
MwoI GCNNNNNNNGC 2 cut(s) 584, 749
NaeI GCCGGC 1 cut(s) 585
NciI CCSGG 1 cut(s) 605
NdeII GATC 3 cut(s) 238, 250, 623
NgoMIV GCCGGC 1 cut(s) 583
NlaIII CATG 2 cut(s) 279, 994
NlaIV GGNNCC 1 cut(s) 602
NmeAIII GCCGAG 2 cut(s) 559, 659
NmuCI GTSAC 3 cut(s) 148, 734, 899
OliI CACNNNNGTG 1 cut(s) 246
PaeR7I CTCGAG 1 cut(s) 324
PagI TCATGA 1 cut(s) 990
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 619
PctI GAATGC 2 cut(s) 235, 887
PdiI GCCGGC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 1054
Pfl23II CGTACG 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 594
Ple19I CGATCG 1 cut(s) 626
PleI GAGTC 2 cut(s) 305, 896
PpsI GAGTC 2 cut(s) 305, 896
Psp124BI GAGCTC 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 374
PspFI CCCAGC 1 cut(s) 1040
PspGI CCWGG 1 cut(s) 374
PspLI CGTACG 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 602
PspPI GGNCC 2 cut(s) 607, 1078
PspXI VCTCGAGB 1 cut(s) 324
PvuI CGATCG 1 cut(s) 626
RsaI GTAC 2 cut(s) 383, 466
RsaNI GTAC 2 cut(s) 382, 465
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 680
SacII CCGCGG 1 cut(s) 480
SaqAI TTAA 1 cut(s) 777
Sau3AI GATC 3 cut(s) 238, 250, 623
Sau96I GGNCC 2 cut(s) 607, 1078
SchI GAGTC 2 cut(s) 305, 896
ScrFI CCNGG 2 cut(s) 376, 605
SduI GDGCHC 3 cut(s) 331, 605, 680
SfaNI GCATC 2 cut(s) 830, 1077
Sfr274I CTCGAG 1 cut(s) 324
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 1 cut(s) 607
SlaI CTCGAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 2 cut(s) 324, 889
SmoI CTYRAG 2 cut(s) 324, 889
SpeI ACTAGT 1 cut(s) 245
Sse9I AATT 1 cut(s) 778
SspMI CTAG 2 cut(s) 234, 246
SstI GAGCTC 1 cut(s) 680
StyD4I CCNGG 2 cut(s) 374, 603
StyI CCWWGG 1 cut(s) 825
TaaI ACNGT 2 cut(s) 464, 469
TaiI ACGT 2 cut(s) 454, 639
TaqI TCGA 6 cut(s) 325, 417, 525, 561, 622, 626
TasI AATT 1 cut(s) 778
TauI GCSGC 8 cut(s) 282, 285, 306, 309, 482, 515, 698, 811
TfiI GAWTC 1 cut(s) 1054
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 2 cut(s) 759, 904
TseFI GTSAC 3 cut(s) 148, 734, 899
TseI GCWGC 6 cut(s) 337, 728, 767, 866, 869, 996
Tsp45I GTSAC 3 cut(s) 148, 734, 899
TspDTI ATGAA 2 cut(s) 762, 979
TspGWI ACGGA 2 cut(s) 161, 790
TspRI CASTG 2 cut(s) 759, 904
VpaK11BI GGWCC 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 1 cut(s) 963
XhoI CTCGAG 1 cut(s) 324
XspI CTAG 2 cut(s) 234, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.