MD10G1051300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010139
Physical Location & Seq
Reverse (-)
6820163 .. 6823406
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1051300.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGTGATGATGGAAGGCTGGTCTCCAACCACCGTATCTCCTTTGCTTCTTCGCAATCTCGTAGCCTCCGTCTTTATCTTCGCAGACAAATCCTTCCTAAACTTGTCTCAGAAATCCAAACTCCTCCAACTCGTTCGCTACGTTTTCGTCACTTCCTTCATCTTCCTCCTCCGTTTGCTTCCTTCTCTAAACCCTAACAATACCCAACCCTATCACTATAAGCCTCCCACTTTCAACAAGTCTAGTAACCACAGCACTACTCATGATCATTACGATTACACTCCCGCCTCAAGCGGCAGTGCTGCTACTATTACTTCCGGCGGCGACTCTGCCATCGGCCGAGCGCTTTCGCAGCTGTTGTCCCTCGTCAACGACATCCCCGTAACCTCCCGCAAGTACCAAGTGGTTCGATCTTTAGCAGAAAGCCTCATCGACGACAACCAAAAAGAGGGCGTTGAGGCTTTACGTCAAGTCACCCGTACTGTTCTCTCCGCGGCTTTTTCCACTACTCTCAGCCAGCTTGAAGCCGTCGCCGCCCTAAAACAAGGAGATGTGTCGGGAGGTGATGGCGTTGATTTGTTGGGGCCCGCTCGACCGGCAGAGTACCGGTTGAGCTGGGTTCTACGGGCGGTTCGATCGATTGGGGACGTGACGTGGATAAGAGGGGCGAGGGCGAGCGGGACGAGGAGCTCGGCGGAGAAACTGGCGGCTGAGGTGCTTTGGTTGGCTGAGAAGCTGGCAGCTTGTGACTCGGCTGATGAAGCTGTGCGGAGATGGGCTGCGGCCCTTAATTTAGCTTGGCTCTCTCTTTCGGTTGAGCCGCGCTTACAAGCATCCTTGGTCAAGGTTTCGGCATTGTTGTTCAAGCATGCCAAACACTTGGGAATGGATGAAACTGACGACGAAAGTAAGAAAGAGCAACACAAGCAAACAAAGATGCAGATGTTAATGTCGTGGCTGCCATTGCTATGCAGAGCCAGCAACGGAACCGATGTCCCGGTATTGAGCATCGGAGAAAGGGCCGAGCTGGAGAGAGTATTGGAAGAGACGATAGAGATGCTGGAAGAGGAGGAGGATCAAGAGAAAGTGCTGTCGTTGTGGCTTCACCACTTCACACATTGCTCGTCATCTGATTGGCCCAACCTCCATGCCTCCTATGCTCGCTGGTGTAACGCTTCTCGGAAGCTTTTGCTGCACCACCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

403

Amino Acids

44.69

Weight (kDa)

6.54

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 590, 678
AccII CGCG 2 cut(s) 494, 823
AclWI GGATC 1 cut(s) 1083
AcoI YGGCCR 1 cut(s) 337
AfaI GTAC 3 cut(s) 398, 481, 605
AfeI AGCGCT 1 cut(s) 345
AfiI CCNNNNNNNGG 1 cut(s) 448
AgeI ACCGGT 1 cut(s) 606
AgsI TTSAA 3 cut(s) 235, 524, 865
AjiI CACGTC 2 cut(s) 649, 654
Alw21I GWGCWC 1 cut(s) 692
Alw26I GTCTC 3 cut(s) 27, 111, 1040
AlwI GGATC 1 cut(s) 1083
Aor51HI AGCGCT 1 cut(s) 345
AoxI GGCC 5 cut(s) 337, 584, 783, 1020, 1136
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 6 cut(s) 302, 352, 740, 779, 958, 1192
AsiGI ACCGGT 1 cut(s) 606
AspLEI GCGC 2 cut(s) 346, 825
AspS9I GGNCC 5 cut(s) 584, 585, 784, 1020, 1137
AsuC2I CCSGG 1 cut(s) 998
AsuHPI GGTGA 4 cut(s) 16, 466, 575, 1097
BaeGI GKGCMC 1 cut(s) 588
BaeI ACNNNNGTAYC 2 cut(s) 18, 51
BanII GRGCYC 2 cut(s) 588, 692
Bbv12I GWGCWC 1 cut(s) 692
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 6 cut(s) 289, 364, 752, 766, 945, 1179
BccI CCATC 4 cut(s) 4, 341, 560, 768
BceAI ACGGC 1 cut(s) 512
BcgI CGANNNNNNTGC 2 cut(s) 1039, 1073
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 1 cut(s) 998
BcoDI GTCTC 3 cut(s) 27, 111, 1040
BfaI CTAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 998
BmgBI CACGTC 2 cut(s) 649, 654
BmgT120I GGNCC 5 cut(s) 584, 585, 784, 1020, 1137
BmiI GGNNCC 3 cut(s) 585, 586, 988
BmrFI CCNGG 1 cut(s) 998
BmsI GCATC 4 cut(s) 842, 927, 1017, 1047
BpmI CTGGAG 1 cut(s) 1049
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 998
Bsa29I ATCGAT 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 492, 837
BsaWI WCCGGW 1 cut(s) 606
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 448
Bse118I RCCGGY 2 cut(s) 595, 606
Bse1I ACTGG 1 cut(s) 708
Bse3DI GCAATG 2 cut(s) 962, 1117
BseCI ATCGAT 1 cut(s) 638
BseDI CCNNGG 2 cut(s) 492, 837
BseGI GGATG 3 cut(s) 375, 833, 895
BseLI CCNNNNNNNGG 1 cut(s) 448
BseMI GCAATG 2 cut(s) 962, 1117
BseMII CTCAG 4 cut(s) 122, 526, 702, 720
BseNI ACTGG 1 cut(s) 708
BseRI GAGGAG 5 cut(s) 113, 158, 700, 1082, 1085
BseSI GKGCMC 1 cut(s) 588
BseX3I CGGCCG 1 cut(s) 337
BseXI GCAGC 6 cut(s) 289, 364, 752, 766, 945, 1179
BseYI CCCAGC 1 cut(s) 615
BsgI GTGCAG 1 cut(s) 1178
Bsh1236I CGCG 2 cut(s) 494, 823
Bsh1285I CGRYCG 3 cut(s) 340, 596, 638
BshFI GGCC 5 cut(s) 339, 586, 785, 1022, 1138
BshTI ACCGGT 1 cut(s) 606
BshVI ATCGAT 1 cut(s) 638
BsiEI CGRYCG 3 cut(s) 340, 596, 638
BsiHKAI GWGCWC 1 cut(s) 692
BsiSI CCGG 4 cut(s) 318, 596, 607, 998
BslFI GGGAC 4 cut(s) 346, 659, 694, 980
BslI CCNNNNNNNGG 1 cut(s) 448
BsmAI GTCTC 3 cut(s) 27, 111, 1040
BsmBI CGTCTC 1 cut(s) 1040
BsmFI GGGAC 4 cut(s) 346, 659, 694, 980
BsnI GGCC 5 cut(s) 339, 586, 785, 1022, 1138
Bso31I GGTCTC 1 cut(s) 27
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 2 cut(s) 588, 692
Bsp143I GATC 4 cut(s) 265, 410, 635, 1075
BspANI GGCC 5 cut(s) 339, 586, 785, 1022, 1138
BspCNI CTCAG 4 cut(s) 121, 525, 703, 721
BspDI ATCGAT 1 cut(s) 638
BspFNI CGCG 2 cut(s) 494, 823
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 3 cut(s) 585, 586, 988
BspPI GGATC 1 cut(s) 1083
BspTNI GGTCTC 1 cut(s) 27
BsrBI CCGCTC 2 cut(s) 590, 678
BsrDI GCAATG 2 cut(s) 962, 1117
BsrFI RCCGGY 2 cut(s) 595, 606
BsrI ACTGG 1 cut(s) 708
BssAI RCCGGY 2 cut(s) 595, 606
BssECI CCNNGG 2 cut(s) 492, 837
BssMI GATC 4 cut(s) 265, 410, 635, 1075
BssT1I CCWWGG 1 cut(s) 837
Bst4CI ACNGT 2 cut(s) 34, 484
Bst6I CTCTTC 2 cut(s) 1038, 1059
BstC8I GCNNGC 7 cut(s) 518, 588, 676, 738, 870, 979, 1162
BstDEI CTNAG 4 cut(s) 108, 512, 711, 729
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 3 cut(s) 375, 833, 895
BstFNI CGCG 2 cut(s) 494, 823
BstH2I RGCGCY 1 cut(s) 347
BstHHI GCGC 2 cut(s) 346, 825
BstKTI GATC 4 cut(s) 268, 413, 638, 1078
BstMAI GTCTC 3 cut(s) 27, 111, 1040
BstMBI GATC 4 cut(s) 265, 410, 635, 1075
BstMCI CGRYCG 3 cut(s) 340, 596, 638
BstMWI GCNNNNNNNGC 9 cut(s) 352, 533, 596, 761, 925, 964, 978, 1157, 1192
BstNSI RCATGY 1 cut(s) 872
BstSCI CCNGG 1 cut(s) 996
BstSLI GKGCMC 1 cut(s) 588
BstUI CGCG 2 cut(s) 494, 823
BstV1I GCAGC 6 cut(s) 289, 364, 752, 766, 945, 1179
BstXI CCANNNNNNTGG 1 cut(s) 880
BstZI CGGCCG 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 638
BsuRI GGCC 5 cut(s) 339, 586, 785, 1022, 1138
BsuTUI ATCGAT 1 cut(s) 638
BtgI CCRYGG 1 cut(s) 492
BtrI CACGTC 2 cut(s) 649, 654
BtsCI GGATG 3 cut(s) 375, 833, 895
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 7 cut(s) 518, 588, 676, 738, 870, 979, 1162
CciI TCATGA 1 cut(s) 262
CfoI GCGC 2 cut(s) 346, 825
Cfr10I RCCGGY 2 cut(s) 595, 606
Cfr13I GGNCC 5 cut(s) 584, 585, 784, 1020, 1137
Cfr42I CCGCGG 1 cut(s) 495
ClaI ATCGAT 1 cut(s) 638
Csp6I GTAC 3 cut(s) 397, 480, 604
CspAI ACCGGT 1 cut(s) 606
CviAII CATG 4 cut(s) 263, 869, 1148, 1202
CviQI GTAC 3 cut(s) 397, 480, 604
DdeI CTNAG 4 cut(s) 108, 512, 711, 729
DpnI GATC 4 cut(s) 267, 412, 637, 1077
DpnII GATC 4 cut(s) 265, 410, 635, 1075
EaeI YGGCCR 1 cut(s) 337
EagI CGGCCG 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 1038, 1059
EarI CTCTTC 2 cut(s) 1038, 1059
EciI GGCGGA 1 cut(s) 710
Ecl136II GAGCTC 1 cut(s) 690
EclXI CGGCCG 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 837
Eco24I GRGCYC 2 cut(s) 588, 692
Eco31I GGTCTC 1 cut(s) 27
Eco47III AGCGCT 1 cut(s) 345
Eco52I CGGCCG 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 690
EcoICRI GAGCTC 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 584
EcoT14I CCWWGG 1 cut(s) 837
EcoT38I GRGCYC 2 cut(s) 588, 692
ErhI CCWWGG 1 cut(s) 837
Esp3I CGTCTC 1 cut(s) 1040
FaeI CATG 4 cut(s) 266, 872, 1151, 1205
FaiI YATR 7 cut(s) 219, 264, 870, 970, 1149, 1158, 1203
FaqI GGGAC 4 cut(s) 346, 659, 694, 980
FatI CATG 4 cut(s) 262, 868, 1147, 1201
FauI CCCGC 4 cut(s) 292, 398, 595, 671
FbaI TGATCA 1 cut(s) 265
FokI GGATG 3 cut(s) 362, 820, 902
FriOI GRGCYC 2 cut(s) 588, 692
FspBI CTAG 1 cut(s) 243
GlaI GCGC 2 cut(s) 345, 824
GsaI CCCAGC 1 cut(s) 619
GsuI CTGGAG 1 cut(s) 1049
HaeII RGCGCY 1 cut(s) 347
HaeIII GGCC 5 cut(s) 339, 586, 785, 1022, 1138
HapII CCGG 4 cut(s) 318, 596, 607, 998
HhaI GCGC 2 cut(s) 346, 825
Hin1II CATG 4 cut(s) 266, 872, 1151, 1205
Hin6I GCGC 2 cut(s) 344, 823
HinP1I GCGC 2 cut(s) 344, 823
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 1184
HinfI GANTC 2 cut(s) 326, 749
HpaII CCGG 4 cut(s) 318, 596, 607, 998
HphI GGTGA 4 cut(s) 16, 466, 575, 1097
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 4 cut(s) 111, 1013, 1132, 1182
Hpy188III TCNNGA 3 cut(s) 263, 558, 1079
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 3 cut(s) 437, 533, 905
HpyAV CCTTC 4 cut(s) 8, 103, 166, 192
HpyCH4III ACNGT 2 cut(s) 34, 484
HpyCH4IV ACGT 4 cut(s) 141, 466, 648, 653
HpyCH4V TGCA 3 cut(s) 940, 972, 1195
HpyF10VI GCNNNNNNNGC 9 cut(s) 352, 533, 596, 761, 925, 964, 978, 1157, 1192
HpyF3I CTNAG 4 cut(s) 108, 512, 711, 729
HpySE526I ACGT 4 cut(s) 141, 466, 648, 653
Hsp92II CATG 4 cut(s) 266, 872, 1151, 1205
HspAI GCGC 2 cut(s) 344, 823
Ksp22I TGATCA 1 cut(s) 265
KspI CCGCGG 1 cut(s) 495
Kzo9I GATC 4 cut(s) 265, 410, 635, 1075
LmnI GCTCC 1 cut(s) 687
Lsp1109I GCAGC 6 cut(s) 289, 364, 752, 766, 945, 1179
LweI GCATC 4 cut(s) 842, 927, 1017, 1047
MaeI CTAG 1 cut(s) 243
MaeII ACGT 4 cut(s) 141, 466, 648, 653
MaeIII GTNAC 7 cut(s) 148, 245, 382, 472, 649, 746, 1169
MalI GATC 4 cut(s) 267, 412, 637, 1077
MbiI CCGCTC 2 cut(s) 590, 678
MboI GATC 4 cut(s) 265, 410, 635, 1075
MboII GAAGA 5 cut(s) 41, 70, 154, 1055, 1076
MhlI GDGCHC 2 cut(s) 588, 692
MluCI AATT 1 cut(s) 790
MlyI GAGTC 2 cut(s) 320, 743
MmeI TCCRAC 2 cut(s) 50, 151
MseI TTAA 2 cut(s) 789, 947
MslI CAYNNNNRTG 2 cut(s) 967, 1200
MspA1I CMGCKG 2 cut(s) 355, 494
MspI CCGG 4 cut(s) 318, 596, 607, 998
MspR9I CCNGG 1 cut(s) 998
MvnI CGCG 2 cut(s) 494, 823
MwoI GCNNNNNNNGC 9 cut(s) 352, 533, 596, 761, 925, 964, 978, 1157, 1192
NciI CCSGG 1 cut(s) 998
NdeII GATC 4 cut(s) 265, 410, 635, 1075
NlaIII CATG 4 cut(s) 266, 872, 1151, 1205
NlaIV GGNNCC 3 cut(s) 585, 586, 988
NmeAIII GCCGAG 4 cut(s) 365, 671, 731, 1048
NmuCI GTSAC 4 cut(s) 148, 472, 649, 746
NspI RCATGY 1 cut(s) 872
PaeI GCATGC 1 cut(s) 872
PagI TCATGA 1 cut(s) 262
PcsI WCGNNNNNNNCGW 4 cut(s) 66, 138, 378, 631
PinAI ACCGGT 1 cut(s) 606
Ple19I CGATCG 1 cut(s) 638
PleI GAGTC 2 cut(s) 320, 743
PpsI GAGTC 2 cut(s) 320, 743
Psp124BI GAGCTC 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 615
PspN4I GGNNCC 3 cut(s) 585, 586, 988
PspOMI GGGCCC 1 cut(s) 584
PspPI GGNCC 5 cut(s) 584, 585, 784, 1020, 1137
PvuI CGATCG 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 3 cut(s) 398, 481, 605
RsaNI GTAC 3 cut(s) 397, 480, 604
RseI CAYNNNNRTG 2 cut(s) 967, 1200
SacI GAGCTC 1 cut(s) 692
SacII CCGCGG 1 cut(s) 495
SaqAI TTAA 2 cut(s) 789, 947
Sau3AI GATC 4 cut(s) 265, 410, 635, 1075
Sau96I GGNCC 5 cut(s) 584, 585, 784, 1020, 1137
SchI GAGTC 2 cut(s) 320, 743
ScrFI CCNGG 1 cut(s) 998
SduI GDGCHC 2 cut(s) 588, 692
SfaNI GCATC 4 cut(s) 842, 927, 1017, 1047
Sfr303I CCGCGG 1 cut(s) 495
SgrBI CCGCGG 1 cut(s) 495
SmiMI CAYNNNNRTG 2 cut(s) 967, 1200
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
SphI GCATGC 1 cut(s) 872
Sse9I AATT 1 cut(s) 790
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 692
StyD4I CCNGG 1 cut(s) 996
StyI CCWWGG 1 cut(s) 837
TaaI ACNGT 2 cut(s) 34, 484
TaiI ACGT 4 cut(s) 144, 469, 651, 656
TaqI TCGA 5 cut(s) 409, 432, 592, 634, 638
TasI AATT 1 cut(s) 790
TauI GCSGC 7 cut(s) 297, 324, 497, 536, 710, 785, 823
Tru1I TTAA 2 cut(s) 789, 947
Tru9I TTAA 2 cut(s) 789, 947
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 4 cut(s) 148, 472, 649, 746
TseI GCWGC 6 cut(s) 302, 352, 740, 779, 958, 1192
Tsp45I GTSAC 4 cut(s) 148, 472, 649, 746
TspDTI ATGAA 3 cut(s) 148, 774, 906
TspGWI ACGGA 3 cut(s) 58, 161, 999
TspRI CASTG 1 cut(s) 304
XceI RCATGY 1 cut(s) 872
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.