AT5G64700

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006280
Physical Location & Seq
Reverse (-)
25864937 .. 25867025
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64700.2

Sequence Viewer

Length: 1080 bp
ATGGACATGGAGTCGAAGAAACCATATCTGATGGTTACTATAATACAAGTCATATATACAATCATGTTCTTGATCTCCAAAGCCGTCTTCAACGGTGGAATGAACACTTTCGTCTTTGTTTTCTATCGTCAAGCTTTCGCTACCATCTTCTTGGCTCCTCTGGCTTTCTTCTTTGAGCGGAAAAGTGCTCCACCTCTTTCATTTGTGACCTTCATCAAGATCTTCATGCTCTCTTTATTTGGTGTGACATTGAGCTTGGACTTAAACGGTATCGCATTATCATACACGTCGGCAACTTTAGCCGCCGCCACAACAGCTTCCCTTCCGGCCATCACATTCTTCTTGGCTCTTTTATTTGGAATGGAGAGGCTTAAGGTTAAGAGCATACAAGGAACAGCAAAGCTTGTGGGGATTACTGTGTGCATGGGAGGGGTAATTATCTTAGCTATTTACAAAGGTCCATTACTGAAATTACCTTTATGCCCTCATTTTTACCACGGCCAAGAACATCCTCACCGGAATAATCCCGGCCACGTCTCCGGCGGTTCCACCTCGTGGCTCAAAGGATGTGTCCTCATGATAACCTCCAACATTCTATGGGGTCTCTGGCTCGTTTTACAGGGTCGTGTGTTGAAGGTTTATCCTTCGAAGCTATATTTCACAACTCTTCATTGTCTATTGAGTTCCATTCAATCATTCGTCATCGCGATTGCTTTAGAGAGAGATATTTCAGCATGGAAGCTTGGTTGGAACCTAAGACTCGTTGCAGTCATTTACTGCGGATTCATTGTAACAGGCGTAGCATATTATTTGCAATCATGGGTTATAGAAAAGAGAGGACCTGTCTTCTTATCAATGTTTACTCCTTTGTCTCTCCTCTTCACTCTCCTCTCTTCAGCGATTCTCTTGTGCGAGATCATCAGTCTTGGAAGTATTGTAGGTGGATTATTATTGATCATAGGACTTTACTGTGTGCTGTGGGGGAAAAGCAGAGAGGAGAAAAACAGTGGTGATGATAAGATTGATTTACAGAAGGAAAACGATGTCGTGTGCAATGAAGTGAAGGTTGTCATTAGTTGA

Protein Analysis

359

Amino Acids

39.75

Weight (kDa)

9.25

Isoelectric Point (pI)

28.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 9 - 145 5.1e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 555
AccBSI CCGCTC 1 cut(s) 178
AccII CGCG 1 cut(s) 707
AciI CCGC 5 cut(s) 178, 303, 306, 543, 780
AcoI YGGCCR 3 cut(s) 327, 499, 529
AcuI CTGAAG 1 cut(s) 879
AdeI CACNNNGTG 1 cut(s) 555
AfiI CCNNNNNNNGG 1 cut(s) 555
AflII CTTAAG 1 cut(s) 371
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 3 cut(s) 91, 634, 692
AhdI GACNNNNNGTC 1 cut(s) 10
AjiI CACGTC 2 cut(s) 288, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 7 cut(s) 134, 255, 317, 403, 446, 652, 742
AluI AGCT 7 cut(s) 134, 255, 317, 403, 446, 652, 742
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 3 cut(s) 541, 608, 876
AoxI GGCC 3 cut(s) 327, 499, 529
ArsI GACNNNNNNTTYG 2 cut(s) 555, 587
Asp700I GAANNNNTTC 1 cut(s) 107
AspS9I GGNCC 2 cut(s) 458, 839
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 2 cut(s) 506, 1022
AsuII TTCGAA 1 cut(s) 647
AvaII GGWCC 2 cut(s) 458, 839
BauI CACGAG 1 cut(s) 553
BbsI GAAGAC 2 cut(s) 79, 838
Bbv12I GWGCWC 1 cut(s) 190
BccI CCATC 3 cut(s) 25, 152, 338
BceAI ACGGC 2 cut(s) 68, 514
BclI TGATCA 1 cut(s) 954
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 3 cut(s) 541, 608, 876
BfrI CTTAAG 1 cut(s) 371
BglII AGATCT 1 cut(s) 219
BisI GCNGC 2 cut(s) 303, 306
BlsI GCNGC 2 cut(s) 304, 307
Bme1390I CCNGG 1 cut(s) 528
Bme18I GGWCC 2 cut(s) 458, 839
BmeRI GACNNNNNGTC 1 cut(s) 10
BmgBI CACGTC 2 cut(s) 288, 535
BmgT120I GGNCC 2 cut(s) 458, 839
BmiI GGNNCC 3 cut(s) 156, 547, 752
BmrFI CCNGG 1 cut(s) 528
BpiI GAAGAC 2 cut(s) 79, 838
Bpu14I TTCGAA 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 1 cut(s) 496
BsaWI WCCGGW 1 cut(s) 516
Bsc4I CCNNNNNNNGG 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 1060
BseDI CCNNGG 1 cut(s) 496
BseGI GGATG 2 cut(s) 508, 572
BseLI CCNNNNNNNGG 1 cut(s) 555
BseMI GCAATG 1 cut(s) 1060
BseRI GAGGAG 4 cut(s) 147, 866, 878, 1010
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 3 cut(s) 329, 501, 531
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 4 cut(s) 326, 517, 528, 540
BslI CCNNNNNNNGG 1 cut(s) 555
BsmAI GTCTC 3 cut(s) 541, 608, 876
BsmBI CGTCTC 1 cut(s) 541
BsnI GGCC 3 cut(s) 329, 501, 531
Bso31I GGTCTC 1 cut(s) 608
Bsp119I TTCGAA 1 cut(s) 647
Bsp1286I GDGCHC 1 cut(s) 190
Bsp143I GATC 4 cut(s) 72, 219, 915, 954
Bsp68I TCGCGA 1 cut(s) 707
BspACI CCGC 5 cut(s) 178, 303, 306, 543, 780
BspANI GGCC 3 cut(s) 329, 501, 531
BspFNI CGCG 1 cut(s) 707
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 3 cut(s) 156, 547, 752
BspT104I TTCGAA 1 cut(s) 647
BspTI CTTAAG 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 1060
BssECI CCNNGG 1 cut(s) 496
BssMI GATC 4 cut(s) 72, 219, 915, 954
BssSI CACGAG 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 553
Bst4CI ACNGT 5 cut(s) 95, 269, 418, 971, 1007
Bst6I CTCTTC 3 cut(s) 672, 884, 898
BstAFI CTTAAG 1 cut(s) 371
BstBI TTCGAA 1 cut(s) 647
BstDEI CTNAG 2 cut(s) 442, 755
BstDSI CCRYGG 1 cut(s) 496
BstF5I GGATG 2 cut(s) 508, 572
BstFNI CGCG 1 cut(s) 707
BstKTI GATC 4 cut(s) 75, 222, 918, 957
BstMAI GTCTC 3 cut(s) 541, 608, 876
BstMBI GATC 4 cut(s) 72, 219, 915, 954
BstMWI GCNNNNNNNGC 3 cut(s) 161, 299, 314
BstSCI CCNGG 1 cut(s) 526
BstUI CGCG 1 cut(s) 707
BstV2I GAAGAC 2 cut(s) 79, 838
BstX2I RGATCY 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 219
BsuRI GGCC 3 cut(s) 329, 501, 531
BtgI CCRYGG 1 cut(s) 496
BtgZI GCGATG 1 cut(s) 688
BtrI CACGTC 2 cut(s) 288, 535
BtsCI GGATG 2 cut(s) 508, 572
BtsIMutI CAGTG 1 cut(s) 1012
BtuMI TCGCGA 1 cut(s) 707
CciI TCATGA 1 cut(s) 576
Cfr13I GGNCC 2 cut(s) 458, 839
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CviAII CATG 7 cut(s) 7, 64, 226, 424, 577, 735, 819
DdeI CTNAG 2 cut(s) 442, 755
DpnI GATC 4 cut(s) 74, 221, 917, 956
DpnII GATC 4 cut(s) 72, 219, 915, 954
DraIII CACNNNGTG 1 cut(s) 555
DriI GACNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 3 cut(s) 327, 499, 529
Eam1104I CTCTTC 3 cut(s) 672, 884, 898
Eam1105I GACNNNNNGTC 1 cut(s) 10
EarI CTCTTC 3 cut(s) 672, 884, 898
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 2 cut(s) 458, 839
Eco57I CTGAAG 1 cut(s) 879
EcoO109I RGGNCCY 1 cut(s) 839
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 7 cut(s) 10, 67, 229, 427, 580, 738, 822
FatI CATG 7 cut(s) 6, 63, 225, 423, 576, 734, 818
FbaI TGATCA 1 cut(s) 954
Fnu4HI GCNGC 2 cut(s) 303, 306
FokI GGATG 2 cut(s) 495, 579
Fsp4HI GCNGC 2 cut(s) 303, 306
GluI GCNGC 2 cut(s) 303, 306
HaeIII GGCC 3 cut(s) 329, 501, 531
HapII CCGG 4 cut(s) 326, 517, 528, 540
Hin1II CATG 7 cut(s) 10, 67, 229, 427, 580, 738, 822
HindIII AAGCTT 3 cut(s) 132, 401, 740
HinfI GANTC 4 cut(s) 11, 759, 783, 901
HpaII CCGG 4 cut(s) 326, 517, 528, 540
HphI GGTGA 2 cut(s) 506, 1022
Hpy166II GTNNAC 1 cut(s) 861
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 4 cut(s) 70, 217, 577, 706
Hpy8I GTNNAC 1 cut(s) 861
Hpy99I CGWCG 1 cut(s) 292
HpyAV CCTTC 6 cut(s) 220, 332, 628, 654, 1027, 1057
HpyCH4III ACNGT 5 cut(s) 95, 269, 418, 971, 1007
HpyCH4IV ACGT 2 cut(s) 287, 534
HpyCH4V TGCA 4 cut(s) 423, 767, 814, 1053
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 299, 314
HpyF3I CTNAG 2 cut(s) 442, 755
HpySE526I ACGT 2 cut(s) 287, 534
Hsp92II CATG 7 cut(s) 10, 67, 229, 427, 580, 738, 822
Ksp22I TGATCA 1 cut(s) 954
Kzo9I GATC 4 cut(s) 72, 219, 915, 954
LmnI GCTCC 2 cut(s) 160, 193
LpnPI CCDG 9 cut(s) 146, 339, 530, 541, 553, 592, 605, 780, 855
MaeII ACGT 2 cut(s) 287, 534
MaeIII GTNAC 4 cut(s) 34, 205, 244, 790
MalI GATC 4 cut(s) 74, 221, 917, 956
MbiI CCGCTC 1 cut(s) 178
MboI GATC 4 cut(s) 72, 219, 915, 954
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 1 cut(s) 190
MluCI AATT 2 cut(s) 435, 470
MlyI GAGTC 2 cut(s) 20, 753
MmeI TCCRAC 2 cut(s) 612, 728
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 3 cut(s) 263, 372, 378
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 4 cut(s) 326, 517, 528, 540
MspR9I CCNGG 1 cut(s) 528
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 3 cut(s) 161, 299, 314
NciI CCSGG 1 cut(s) 528
NdeII GATC 4 cut(s) 72, 219, 915, 954
NlaIII CATG 7 cut(s) 10, 67, 229, 427, 580, 738, 822
NlaIV GGNNCC 3 cut(s) 156, 547, 752
NmuCI GTSAC 2 cut(s) 205, 244
NruI TCGCGA 1 cut(s) 707
NspV TTCGAA 1 cut(s) 647
PagI TCATGA 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 2 cut(s) 783, 901
PflMI CCANNNNNTGG 1 cut(s) 555
PkrI GCNGC 2 cut(s) 304, 307
PleI GAGTC 2 cut(s) 19, 753
PpsI GAGTC 2 cut(s) 19, 753
PpuMI RGGWCCY 1 cut(s) 839
Psp5II RGGWCCY 1 cut(s) 839
PspN4I GGNNCC 3 cut(s) 156, 547, 752
PspPI GGNCC 2 cut(s) 458, 839
PspPPI RGGWCCY 1 cut(s) 839
PsuI RGATCY 1 cut(s) 219
RruI TCGCGA 1 cut(s) 707
SaqAI TTAA 3 cut(s) 263, 372, 378
SatI GCNGC 2 cut(s) 303, 306
Sau3AI GATC 4 cut(s) 72, 219, 915, 954
Sau96I GGNCC 2 cut(s) 458, 839
SchI GAGTC 2 cut(s) 20, 753
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 190
SfuI TTCGAA 1 cut(s) 647
SinI GGWCC 2 cut(s) 458, 839
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 2 cut(s) 435, 470
SsiI CCGC 5 cut(s) 178, 303, 306, 543, 780
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 5 cut(s) 95, 269, 418, 971, 1007
TaiI ACGT 2 cut(s) 290, 537
TaqI TCGA 2 cut(s) 14, 647
TasI AATT 2 cut(s) 435, 470
TauI GCSGC 2 cut(s) 305, 308
TfiI GAWTC 2 cut(s) 783, 901
Tru1I TTAA 3 cut(s) 263, 372, 378
Tru9I TTAA 3 cut(s) 263, 372, 378
TscAI CASTG 1 cut(s) 1012
TseFI GTSAC 2 cut(s) 205, 244
Tsp45I GTSAC 2 cut(s) 205, 244
TspDTI ATGAA 7 cut(s) 116, 189, 202, 214, 659, 775, 1071
TspRI CASTG 1 cut(s) 1012
Van91I CCANNNNNTGG 1 cut(s) 555
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 2 cut(s) 458, 839
XmnI GAANNNNTTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.