AT5G64816

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005024
Physical Location & Seq
Forward (+)
25912969 .. 25914027
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64816.2

Sequence Viewer

Length: 393 bp
ATGGTGGAGGTGTGGTGGTCACTGATAGGAGCAGCAGTGCCTGCACTAATTGCTGGTCAAGCTTGGAGGATAAAGAAGAGACATGGCGAAGAAGAGAGGATCAAGAGTGCTCGAGGAAGAGAGAAAAGCTCTGATGAAATCTTTGTCTGTGAAAGAGTTTGCACTTCCAAAAGAATGCTGAAAAAAGTTGGAGCTTTCTCTAAAGATCCTATCCCCGACACTTGTGTTACTGTCTGCGGTGTATCTGAGCTCGACGCTTGCTCTGATGCTTGTGCTCGAACTGTTTGCGTTAACCAGCATCAAGTTGCTAATTGGAATGACATTTGTCTCAGAAGATGTCAAAGTGAATGTCTTAAGCTCTCTTCTTCTTCTTCTTCTTCTCGCTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.44

Weight (kDa)

8.73

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 237
AclWI GGATC 2 cut(s) 107, 200
AflII CTTAAG 1 cut(s) 353
AluBI AGCT 5 cut(s) 62, 129, 194, 250, 358
AluI AGCT 5 cut(s) 62, 129, 194, 250, 358
Alw21I GWGCWC 3 cut(s) 112, 252, 277
Alw26I GTCTC 2 cut(s) 73, 332
AlwI GGATC 2 cut(s) 107, 200
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 111
ApeKI GCWGC 1 cut(s) 32
ArsI GACNNNNNNTTYG 2 cut(s) 334, 366
AvaI CYCGRG 1 cut(s) 111
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 3 cut(s) 112, 252, 277
BbvI GCAGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 267, 301
BcoDI GTCTC 2 cut(s) 73, 332
BfrI CTTAAG 1 cut(s) 353
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 111
BmsI GCATC 2 cut(s) 256, 307
BoxI GACNNNNGTC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BsaXI ACNNNNNCTCC 3 cut(s) 21, 29, 51
BseMII CTCAG 2 cut(s) 237, 343
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 27
BsiHKAI GWGCWC 3 cut(s) 112, 252, 277
BsiHKCI CYCGRG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 73, 332
BsmI GAATGC 1 cut(s) 180
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 112, 252, 277
Bsp143I GATC 2 cut(s) 99, 205
BspACI CCGC 1 cut(s) 237
BspCNI CTCAG 2 cut(s) 238, 342
BspPI GGATC 2 cut(s) 107, 200
BspTI CTTAAG 1 cut(s) 353
BssMI GATC 2 cut(s) 99, 205
Bst4CI ACNGT 2 cut(s) 232, 283
Bst6I CTCTTC 4 cut(s) 71, 87, 112, 367
BstAFI CTTAAG 1 cut(s) 353
BstAPI GCANNNNNTGC 2 cut(s) 41, 50
BstC8I GCNNGC 2 cut(s) 42, 259
BstDEI CTNAG 2 cut(s) 246, 329
BstKTI GATC 2 cut(s) 102, 208
BstMAI GTCTC 2 cut(s) 73, 332
BstMBI GATC 2 cut(s) 99, 205
BstMWI GCNNNNNNNGC 3 cut(s) 41, 50, 59
BstPAI GACNNNNGTC 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 44
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 2 cut(s) 20, 42
Cac8I GCNNGC 2 cut(s) 42, 259
CaiI CAGNNNCTG 1 cut(s) 41
CseI GACGC 1 cut(s) 263
CviAII CATG 1 cut(s) 83
CviJI RGCY 5 cut(s) 62, 129, 194, 250, 358
CviKI_1 RGCY 5 cut(s) 62, 129, 194, 250, 358
DdeI CTNAG 2 cut(s) 246, 329
DpnI GATC 2 cut(s) 101, 207
DpnII GATC 2 cut(s) 99, 205
Eam1104I CTCTTC 4 cut(s) 71, 87, 112, 367
EarI CTCTTC 4 cut(s) 71, 87, 112, 367
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 86
FaiI YATR 1 cut(s) 84
FatI CATG 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 33
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 33
HgaI GACGC 1 cut(s) 263
Hin1II CATG 1 cut(s) 86
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 1 cut(s) 60
HpaI GTTAAC 1 cut(s) 292
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 4 cut(s) 133, 247, 265, 332
Hpy188III TCNNGA 2 cut(s) 103, 390
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 1 cut(s) 257
HpyCH4III ACNGT 2 cut(s) 232, 283
HpyCH4V TGCA 2 cut(s) 44, 162
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 50, 59
HpyF3I CTNAG 2 cut(s) 246, 329
Hsp92II CATG 1 cut(s) 86
KspAI GTTAAC 1 cut(s) 292
Kzo9I GATC 2 cut(s) 99, 205
LmnI GCTCC 2 cut(s) 29, 191
LpnPI CCDG 3 cut(s) 39, 54, 308
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 2 cut(s) 256, 307
MaeIII GTNAC 2 cut(s) 18, 226
MalI GATC 2 cut(s) 101, 207
MboI GATC 2 cut(s) 99, 205
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 3 cut(s) 112, 252, 277
MluCI AATT 2 cut(s) 48, 310
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 3 cut(s) 60, 90, 107
MseI TTAA 2 cut(s) 291, 354
MspCI CTTAAG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 180
MwoI GCNNNNNNNGC 3 cut(s) 41, 50, 59
NdeII GATC 2 cut(s) 99, 205
NlaIII CATG 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 111
PctI GAATGC 1 cut(s) 180
PkrI GCNGC 1 cut(s) 34
PshAI GACNNNNGTC 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 252
PspXI VCTCGAGB 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 205
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 291, 354
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 2 cut(s) 99, 205
SduI GDGCHC 3 cut(s) 112, 252, 277
SetI ASST 6 cut(s) 12, 64, 131, 196, 252, 360
SfaNI GCATC 2 cut(s) 256, 307
Sfr274I CTCGAG 1 cut(s) 111
SlaI CTCGAG 1 cut(s) 111
SmlI CTYRAG 2 cut(s) 111, 353
SmoI CTYRAG 2 cut(s) 111, 353
Sse9I AATT 2 cut(s) 48, 310
SsiI CCGC 1 cut(s) 237
SstI GAGCTC 1 cut(s) 252
TaaI ACNGT 2 cut(s) 232, 283
TaqI TCGA 3 cut(s) 112, 252, 277
TasI AATT 2 cut(s) 48, 310
Tru1I TTAA 2 cut(s) 291, 354
Tru9I TTAA 2 cut(s) 291, 354
TscAI CASTG 2 cut(s) 27, 42
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 2 cut(s) 27, 42
Vha464I CTTAAG 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.