Prupe.6G170300_v2.0.a1

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0005024
Physical Location & Seq
Forward (+)
17547046 .. 17548722
1677 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G170300.2

Sequence Viewer

Length: 378 bp
ATGGTGGAAGTGTGGTGGTCCCTATTAGCTGCAATCCCCGCATTGGTTGCAGGACAAGCATTTCGAGTGAAGAAAAGGCGTGCTGAAGAGCAGAGATTAAAGAGTGCCAGGGGGAGAGAGAAGAATTCTGATGAGATTTTTGTCTGTGAGAGGGTATGTACATCCAAGAGAATGCTGAAAAAGCTAGGTGCATTCTCAAAGGACCCAATTCCTGATACTTGTGTCACTGTGTGTGGCGTGTCGGAGCTGGATGCGTGTGCTGATGCATGCGCACGCACTGTTTGCGTAAACCAACATCAAGTACCCAACTGGAATGACAAATGCCTTCGGAGATGCCAGAGTGAATGTCTAAAACTCTCTTCTTCCAGTTATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.01

Weight (kDa)

9.0

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 271
AciI CCGC 1 cut(s) 39
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 231
AfaI GTAC 2 cut(s) 160, 303
AfiI CCNNNNNNNGG 1 cut(s) 43
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 3 cut(s) 29, 184, 247
AluI AGCT 3 cut(s) 29, 184, 247
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 124
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 18, 202
AvaII GGWCC 2 cut(s) 18, 202
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BbvI GCAGC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 109
BfaI CTAG 1 cut(s) 185
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 2 cut(s) 18, 202
BmgT120I GGNCC 2 cut(s) 18, 202
BmiI GGNNCC 2 cut(s) 20, 204
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 3 cut(s) 241, 253, 323
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 2 cut(s) 314, 366
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 161, 256
BseLI CCNNNNNNNGG 1 cut(s) 43
BseNI ACTGG 2 cut(s) 314, 366
BseXI GCAGC 1 cut(s) 16
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 4
BsmI GAATGC 2 cut(s) 177, 191
Bsp1407I TGTACA 1 cut(s) 158
BspACI CCGC 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 20, 204
BspQI GCTCTTC 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 2 cut(s) 314, 366
BssECI CCNNGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 2 cut(s) 229, 280
Bst6I CTCTTC 2 cut(s) 81, 364
BstAPI GCANNNNNTGC 2 cut(s) 47, 282
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 81, 268, 274
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 161, 256
BstHHI GCGC 1 cut(s) 272
BstMWI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 181, 282
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 16
BtsCI GGATG 2 cut(s) 161, 256
BtsIMutI CAGTG 2 cut(s) 225, 276
Cac8I GCNNGC 3 cut(s) 81, 268, 274
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 18, 202
Csp6I GTAC 2 cut(s) 159, 302
CviAII CATG 1 cut(s) 267
CviJI RGCY 3 cut(s) 29, 184, 247
CviKI_1 RGCY 3 cut(s) 29, 184, 247
CviQI GTAC 2 cut(s) 159, 302
DdeI CTNAG 1 cut(s) 375
DraIII CACNNNGTG 1 cut(s) 231
Eam1104I CTCTTC 2 cut(s) 81, 364
EarI CTCTTC 2 cut(s) 81, 364
Eco47I GGWCC 2 cut(s) 18, 202
Eco57I CTGAAG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 107
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 1 cut(s) 270
FaiI YATR 2 cut(s) 157, 268
FaqI GGGAC 1 cut(s) 4
FatI CATG 1 cut(s) 266
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 148, 263
Fsp4HI GCNGC 1 cut(s) 30
FspAI RTGCGCAY 1 cut(s) 271
FspBI CTAG 1 cut(s) 185
FspI TGCGCA 1 cut(s) 271
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 272
Hin1II CATG 1 cut(s) 270
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 130, 244, 330
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 2 cut(s) 229, 280
HpyCH4V TGCA 4 cut(s) 32, 50, 191, 266
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 181, 282
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 1 cut(s) 270
HspAI GCGC 1 cut(s) 270
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 7 cut(s) 36, 94, 121, 225, 233, 295, 350
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 241, 253, 323
MaeI CTAG 1 cut(s) 185
MaeIII GTNAC 1 cut(s) 223
MboII GAAGA 5 cut(s) 82, 98, 133, 351, 354
MluCI AATT 2 cut(s) 124, 207
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 1 cut(s) 144
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 109
Mva1269I GAATGC 2 cut(s) 177, 191
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 5 cut(s) 38, 47, 56, 181, 282
NlaIII CATG 1 cut(s) 270
NlaIV GGNNCC 2 cut(s) 20, 204
NmuCI GTSAC 1 cut(s) 223
NsbI TGCGCA 1 cut(s) 271
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 1 cut(s) 270
PaeI GCATGC 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 81
PctI GAATGC 2 cut(s) 177, 191
PkrI GCNGC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 2 cut(s) 20, 204
PspPI GGNCC 2 cut(s) 18, 202
PspPPI RGGWCCY 1 cut(s) 202
RsaI GTAC 2 cut(s) 160, 303
RsaNI GTAC 2 cut(s) 159, 302
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 1 cut(s) 30
Sau96I GGNCC 2 cut(s) 18, 202
ScrFI CCNGG 1 cut(s) 109
SetI ASST 4 cut(s) 31, 186, 190, 249
SfaNI GCATC 3 cut(s) 241, 253, 323
SinI GGWCC 2 cut(s) 18, 202
SphI GCATGC 1 cut(s) 270
Sse9I AATT 2 cut(s) 124, 207
SsiI CCGC 1 cut(s) 39
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 229, 280
TaqI TCGA 1 cut(s) 64
TasI AATT 2 cut(s) 124, 207
TatI WGTACW 1 cut(s) 158
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 2 cut(s) 232, 283
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 223
TspRI CASTG 2 cut(s) 232, 283
VpaK11BI GGWCC 2 cut(s) 18, 202
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 270
XspI CTAG 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.