pycom03g18380

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005024
Physical Location & Seq
Forward (+)
19357859 .. 19358236
378 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g18380.1

Sequence Viewer

Length: 378 bp
ATGGTGGAAGTGTGGTGGTCCCTCCTAGCTGCTATCCCTGCAGTGGTTGCAGGACAAGCATTTCGAGTGAAGAAAAGGCACGCCGAAGAGCAGAGATTAAAGAGCGCTAGAGGAAGGGAGAAGAGTTCTGATGAGATTTTTGTCTGCGAAAGGGTATGCACGTCGAAGAGAATGCTGAAAAAGGTTGGTGCATTCTCAAAGGACCCAATTCCTGATACTTGTGTTACCGTCTGCGGTGTATCTGAGCTTGATGCGTGTGCTGATGCGTGCGTGCGCACTGTCTGTGTAAACCAACATCAAGTACCCAACTGGAATGACATCTGCCTTAAGAGATGCCAGAGTGAGTGTTTGAAACTCTCTTCTTCAAGTTCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.87

Weight (kDa)

8.73

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 275
AciI CCGC 1 cut(s) 234
AfaI GTAC 1 cut(s) 303
AfeI AGCGCT 1 cut(s) 106
AfiI CCNNNNNNNGG 1 cut(s) 43
AflII CTTAAG 1 cut(s) 326
AgsI TTSAA 2 cut(s) 352, 366
AjiI CACGTC 1 cut(s) 162
AluBI AGCT 2 cut(s) 29, 247
AluI AGCT 2 cut(s) 29, 247
Aor51HI AGCGCT 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AspLEI GCGC 2 cut(s) 107, 276
AspS9I GGNCC 2 cut(s) 18, 202
AvaII GGWCC 2 cut(s) 18, 202
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BbvI GCAGC 1 cut(s) 16
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BfaI CTAG 2 cut(s) 26, 108
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 108
BfrI CTTAAG 1 cut(s) 326
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 2 cut(s) 18, 202
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 18, 202
BmiI GGNNCC 2 cut(s) 20, 204
BmsI GCATC 3 cut(s) 241, 253, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 16
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 4
BsmI GAATGC 2 cut(s) 177, 191
BspACI CCGC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 20, 204
BspMAI CTGCAG 1 cut(s) 43
BspQI GCTCTTC 1 cut(s) 81
BspTI CTTAAG 1 cut(s) 326
BsrI ACTGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 229, 280
Bst6I CTCTTC 4 cut(s) 81, 116, 161, 364
BstAFI CTTAAG 1 cut(s) 326
BstAPI GCANNNNNTGC 1 cut(s) 47
BstC8I GCNNGC 3 cut(s) 81, 268, 272
BstDEI CTNAG 1 cut(s) 243
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 2 cut(s) 107, 276
BstMWI GCNNNNNNNGC 3 cut(s) 38, 47, 56
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 16
BtrI CACGTC 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 276
Cac8I GCNNGC 3 cut(s) 81, 268, 272
CfoI GCGC 2 cut(s) 107, 276
Cfr13I GGNCC 2 cut(s) 18, 202
Csp6I GTAC 1 cut(s) 302
CviJI RGCY 2 cut(s) 29, 247
CviKI_1 RGCY 2 cut(s) 29, 247
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 1 cut(s) 243
Eam1104I CTCTTC 4 cut(s) 81, 116, 161, 364
EarI CTCTTC 4 cut(s) 81, 116, 161, 364
Eco47I GGWCC 2 cut(s) 18, 202
Eco47III AGCGCT 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 202
FaiI YATR 2 cut(s) 157, 376
FaqI GGGAC 1 cut(s) 4
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
FspAI RTGCGCAY 1 cut(s) 275
FspBI CTAG 2 cut(s) 26, 108
FspI TGCGCA 1 cut(s) 275
GlaI GCGC 2 cut(s) 106, 275
GluI GCNGC 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 108
HhaI GCGC 2 cut(s) 107, 276
Hin6I GCGC 2 cut(s) 105, 274
HinP1I GCGC 2 cut(s) 105, 274
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 130, 244
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 2 cut(s) 229, 280
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 4 cut(s) 41, 50, 159, 191
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 47, 56
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 161
HspAI GCGC 2 cut(s) 105, 274
LguI GCTCTTC 1 cut(s) 81
LpnPI CCDG 5 cut(s) 36, 51, 225, 295, 350
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 241, 253, 323
MaeI CTAG 2 cut(s) 26, 108
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 1 cut(s) 223
MboII GAAGA 6 cut(s) 82, 98, 133, 178, 351, 354
MluCI AATT 1 cut(s) 207
MnlI CCTC 2 cut(s) 32, 104
MseI TTAA 2 cut(s) 98, 327
MspCI CTTAAG 1 cut(s) 326
Mva1269I GAATGC 2 cut(s) 177, 191
MwoI GCNNNNNNNGC 3 cut(s) 38, 47, 56
NlaIV GGNNCC 2 cut(s) 20, 204
NsbI TGCGCA 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 81
PctI GAATGC 2 cut(s) 177, 191
PkrI GCNGC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 20, 204
PspPI GGNCC 2 cut(s) 18, 202
PspPPI RGGWCCY 1 cut(s) 202
PstI CTGCAG 1 cut(s) 43
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 98, 327
SatI GCNGC 1 cut(s) 30
Sau96I GGNCC 2 cut(s) 18, 202
SetI ASST 4 cut(s) 31, 164, 186, 249
SfaNI GCATC 3 cut(s) 241, 253, 323
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 2 cut(s) 18, 202
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 1 cut(s) 207
SsiI CCGC 1 cut(s) 234
SspMI CTAG 2 cut(s) 26, 108
TaaI ACNGT 2 cut(s) 229, 280
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 64, 164
TasI AATT 1 cut(s) 207
Tru1I TTAA 2 cut(s) 98, 327
Tru9I TTAA 2 cut(s) 98, 327
TscAI CASTG 2 cut(s) 48, 283
TseI GCWGC 1 cut(s) 29
TspRI CASTG 2 cut(s) 48, 283
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 18, 202
XspI CTAG 2 cut(s) 26, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.