Rh5CG243700

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005024
Physical Location & Seq
Reverse (-)
25399762 .. 25401869
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG243700.1

Sequence Viewer

Length: 378 bp
ATGGTGGAAGTGTGGTGGTCCCTTTTAGCTGCTATCCCTGCAGTGGTTGCAGGACAAGCATTTCGAGTGAAGAAAAGACGTGATGAAGAGCAGAAATTAAAGAGTGCTAGAGGAAGGGAGAAGAGTTCTGATGAGATTTTTGTCTGCGAGAGGGTATGCACATCTAAGAGGATGCTGAAGAAAGTTGGTGCATTCTCTAAGGACCCAATTCCTGATACTTGTGTTACTGTGTGCGGTGTTTCTGAGCTTGACGTATGCGCTGATGCATGTGCACGGACTATTTGTGTAAACCAACATCAATTACCAAACTGGAATGACATCTGCCTCCGAAGATGCCAGAGTGAGTGTTTGAAACTCTCTTCTTCGACTTCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.98

Weight (kDa)

8.73

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 1 cut(s) 352
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 2 cut(s) 29, 247
AluI AGCT 2 cut(s) 29, 247
Alw21I GWGCWC 1 cut(s) 274
Alw44I GTGCAC 1 cut(s) 270
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 2 cut(s) 18, 202
AvaII GGWCC 2 cut(s) 18, 202
BaeGI GKGCMC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 1 cut(s) 16
BfaI CTAG 2 cut(s) 108, 376
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 2 cut(s) 18, 202
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 18, 202
BmiI GGNNCC 2 cut(s) 20, 204
BmsI GCATC 3 cut(s) 162, 253, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 314
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 1 cut(s) 16
BsiHKAI GWGCWC 1 cut(s) 274
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 4
BsmI GAATGC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 274
BspACI CCGC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 20, 204
BspMAI CTGCAG 1 cut(s) 43
BspQI GCTCTTC 1 cut(s) 81
BsrI ACTGG 1 cut(s) 314
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 3 cut(s) 81, 116, 364
BstAPI GCANNNNNTGC 1 cut(s) 47
BstDEI CTNAG 3 cut(s) 165, 198, 243
BstF5I GGATG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 260
BstMWI GCNNNNNNNGC 3 cut(s) 38, 47, 56
BstNSI RCATGY 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 16
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
CfoI GCGC 1 cut(s) 260
Cfr13I GGNCC 2 cut(s) 18, 202
CviAII CATG 1 cut(s) 267
CviJI RGCY 2 cut(s) 29, 247
CviKI_1 RGCY 2 cut(s) 29, 247
DdeI CTNAG 3 cut(s) 165, 198, 243
Eam1104I CTCTTC 3 cut(s) 81, 116, 364
EarI CTCTTC 3 cut(s) 81, 116, 364
Eco47I GGWCC 2 cut(s) 18, 202
Eco57I CTGAAG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 202
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 1 cut(s) 270
FaiI YATR 3 cut(s) 157, 256, 268
FaqI GGGAC 1 cut(s) 4
FatI CATG 1 cut(s) 266
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 2 cut(s) 108, 376
GlaI GCGC 1 cut(s) 259
GluI GCNGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 260
Hin1II CATG 1 cut(s) 270
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
Hpy166II GTNNAC 2 cut(s) 272, 289
Hpy188I TCNGA 3 cut(s) 130, 244, 329
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 272, 289
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 79, 252
HpyCH4V TGCA 6 cut(s) 41, 50, 159, 191, 266, 272
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 47, 56
HpyF3I CTNAG 3 cut(s) 165, 198, 243
HpySE526I ACGT 2 cut(s) 79, 252
Hsp92II CATG 1 cut(s) 270
HspAI GCGC 1 cut(s) 258
LguI GCTCTTC 1 cut(s) 81
LpnPI CCDG 5 cut(s) 36, 51, 225, 295, 350
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 162, 253, 323
MaeI CTAG 2 cut(s) 108, 376
MaeII ACGT 2 cut(s) 79, 252
MaeIII GTNAC 1 cut(s) 223
MboII GAAGA 7 cut(s) 82, 98, 133, 190, 342, 351, 354
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 3 cut(s) 95, 207, 299
MnlI CCTC 4 cut(s) 104, 144, 162, 335
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 38, 47, 56
NlaIII CATG 1 cut(s) 270
NlaIV GGNNCC 2 cut(s) 20, 204
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 81
PctI GAATGC 1 cut(s) 191
PkrI GCNGC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 20, 204
PspPI GGNCC 2 cut(s) 18, 202
PspPPI RGGWCCY 1 cut(s) 202
PstI CTGCAG 1 cut(s) 43
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 1 cut(s) 30
Sau96I GGNCC 2 cut(s) 18, 202
SduI GDGCHC 1 cut(s) 274
SetI ASST 4 cut(s) 31, 82, 249, 255
SfaNI GCATC 3 cut(s) 162, 253, 323
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 2 cut(s) 18, 202
Sse9I AATT 3 cut(s) 95, 207, 299
SsiI CCGC 1 cut(s) 234
SspMI CTAG 2 cut(s) 108, 376
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 2 cut(s) 82, 255
TaqI TCGA 2 cut(s) 64, 365
TasI AATT 3 cut(s) 95, 207, 299
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 1 cut(s) 48
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 1 cut(s) 48
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 2 cut(s) 18, 202
XceI RCATGY 1 cut(s) 270
XspI CTAG 2 cut(s) 108, 376
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.