RLG00000018328

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005024
Physical Location & Seq
Reverse (-)
28376921 .. 28379446
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018328

Sequence Viewer

Length: 471 bp
ATGTGGAGCTCACAAATCGCCTTGTATTCCAGGTTCATTGTGTTTCTGTATCTGTTAAAGACTGCTGAAGAAGAGATTGACCTTGCATTGGTGATGGTGGATGCGTGGTGGTCCCTGTTAGCTGCTATCCCTGCGGTGGTTGCAGGACAAGCATATCGGGTCAAGAAAAGGCGTGCTGAAGATCAGAGATTAAAGAGTGCTAGGGGAAGGGAGAAGAGTTCTGATGAGATTTTTGTCTGTGAGAGGGTATGCACATCAAAGAGAATGCTCAAAAAGGTAGGTGCATTCTCAAAGGACCCTATTCCCGATACGTGTGTTACTGTATGTGGTGTCTCTGAGCTCGATGCATGCGCTGATGCATGTGCACGCACTGTTTGCGTGAACCAACACCAAGTGCCCAACTGGAATGACATCTGCCTTCGGAGATGCCAGAGTGAATGTCTAAAACTCTCTTCCTCCAGTTCTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.47

Weight (kDa)

8.54

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 134
AcuI CTGAAG 2 cut(s) 87, 198
AdeI CACNNNGTG 1 cut(s) 394
AfiI CCNNNNNNNGG 2 cut(s) 88, 136
AflIII ACRYGT 1 cut(s) 311
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 3 cut(s) 9, 122, 340
AluI AGCT 3 cut(s) 9, 122, 340
Alw21I GWGCWC 3 cut(s) 11, 342, 367
Alw26I GTCTC 1 cut(s) 337
Alw44I GTGCAC 1 cut(s) 363
ApaLI GTGCAC 1 cut(s) 363
ApeKI GCWGC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 2 cut(s) 111, 295
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 2 cut(s) 111, 295
BaeGI GKGCMC 2 cut(s) 367, 399
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BanII GRGCYC 2 cut(s) 11, 342
Bbv12I GWGCWC 3 cut(s) 11, 342, 367
BbvI GCAGC 1 cut(s) 109
BccI CCATC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 1 cut(s) 201
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 2 cut(s) 111, 295
BmgT120I GGNCC 2 cut(s) 111, 295
BmiI GGNNCC 2 cut(s) 113, 297
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 4 cut(s) 91, 334, 346, 416
BpmI CTGGAG 1 cut(s) 442
BsaAI YACGTR 1 cut(s) 312
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 136
Bse1I ACTGG 2 cut(s) 407, 459
BseBI CCWGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 2 cut(s) 88, 136
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 2 cut(s) 407, 459
BseSI GKGCMC 2 cut(s) 367, 399
BseXI GCAGC 1 cut(s) 109
BsiHKAI GWGCWC 3 cut(s) 11, 342, 367
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 2 cut(s) 88, 136
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 2 cut(s) 270, 284
Bsp1286I GDGCHC 4 cut(s) 11, 342, 367, 399
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 113, 297
BsrI ACTGG 2 cut(s) 407, 459
BssMI GATC 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 2 cut(s) 322, 373
Bst6I CTCTTC 3 cut(s) 66, 209, 457
BstAPI GCANNNNNTGC 1 cut(s) 375
BstBAI YACGTR 1 cut(s) 312
BstC8I GCNNGC 3 cut(s) 174, 349, 367
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 4 cut(s) 131, 140, 149, 375
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 2 cut(s) 351, 363
BstSCI CCNGG 1 cut(s) 29
BstSLI GKGCMC 2 cut(s) 367, 399
BstV1I GCAGC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 174, 349, 367
CfoI GCGC 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 111, 295
CviAII CATG 2 cut(s) 348, 360
CviJI RGCY 3 cut(s) 9, 122, 340
CviKI_1 RGCY 3 cut(s) 9, 122, 340
DdeI CTNAG 1 cut(s) 336
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DraIII CACNNNGTG 1 cut(s) 394
Eam1104I CTCTTC 3 cut(s) 66, 209, 457
EarI CTCTTC 3 cut(s) 66, 209, 457
Ecl136II GAGCTC 2 cut(s) 9, 340
Eco24I GRGCYC 2 cut(s) 11, 342
Eco47I GGWCC 2 cut(s) 111, 295
Eco53kI GAGCTC 2 cut(s) 9, 340
Eco57I CTGAAG 2 cut(s) 87, 198
EcoICRI GAGCTC 2 cut(s) 9, 340
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 29
EcoT22I ATGCAT 2 cut(s) 349, 361
EcoT38I GRGCYC 2 cut(s) 11, 342
FaeI CATG 2 cut(s) 351, 363
FaiI YATR 5 cut(s) 154, 250, 325, 349, 361
FaqI GGGAC 1 cut(s) 97
FatI CATG 2 cut(s) 347, 359
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 1 cut(s) 113
FriOI GRGCYC 2 cut(s) 11, 342
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 352
GluI GCNGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 442
HhaI GCGC 1 cut(s) 353
Hin1II CATG 2 cut(s) 351, 363
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 365, 382
Hpy188I TCNGA 4 cut(s) 186, 223, 337, 423
Hpy188III TCNNGA 2 cut(s) 163, 305
Hpy8I GTNNAC 2 cut(s) 365, 382
HpyAV CCTTC 2 cut(s) 201, 428
HpyCH4III ACNGT 2 cut(s) 322, 373
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 7 cut(s) 86, 143, 252, 284, 347, 359, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 140, 149, 375
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 2 cut(s) 351, 363
HspAI GCGC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 6
LpnPI CCDG 7 cut(s) 16, 43, 128, 129, 144, 388, 443
Lsp1109I GCAGC 1 cut(s) 109
LweI GCATC 4 cut(s) 91, 334, 346, 416
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 316
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 6 cut(s) 80, 83, 191, 226, 444, 456
MhlI GDGCHC 4 cut(s) 11, 342, 367, 399
MnlI CCTC 2 cut(s) 237, 466
Mph1103I ATGCAT 2 cut(s) 349, 361
MseI TTAA 2 cut(s) 56, 191
MspR9I CCNGG 1 cut(s) 31
Mva1269I GAATGC 2 cut(s) 270, 284
MvaI CCWGG 1 cut(s) 31
MwoI GCNNNNNNNGC 4 cut(s) 131, 140, 149, 375
NdeII GATC 1 cut(s) 181
NlaIII CATG 2 cut(s) 351, 363
NlaIV GGNNCC 2 cut(s) 113, 297
NsiI ATGCAT 2 cut(s) 349, 361
NspI RCATGY 2 cut(s) 351, 363
PaeI GCATGC 1 cut(s) 351
PctI GAATGC 2 cut(s) 270, 284
PkrI GCNGC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 295
Psp124BI GAGCTC 2 cut(s) 11, 342
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 2 cut(s) 113, 297
PspPI GGNCC 2 cut(s) 111, 295
PspPPI RGGWCCY 1 cut(s) 295
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
SacI GAGCTC 2 cut(s) 11, 342
SaqAI TTAA 2 cut(s) 56, 191
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 2 cut(s) 111, 295
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 4 cut(s) 11, 342, 367, 399
SetI ASST 8 cut(s) 11, 35, 84, 124, 279, 283, 314, 342
SfaNI GCATC 4 cut(s) 91, 334, 346, 416
SinI GGWCC 2 cut(s) 111, 295
SphI GCATGC 1 cut(s) 351
SsiI CCGC 1 cut(s) 134
SspMI CTAG 1 cut(s) 201
SstI GAGCTC 2 cut(s) 11, 342
StyD4I CCNGG 1 cut(s) 29
TaaI ACNGT 2 cut(s) 322, 373
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 342
Tru1I TTAA 2 cut(s) 56, 191
Tru9I TTAA 2 cut(s) 56, 191
TscAI CASTG 1 cut(s) 376
TseI GCWGC 1 cut(s) 122
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 1 cut(s) 376
VneI GTGCAC 1 cut(s) 363
VpaK11BI GGWCC 2 cut(s) 111, 295
XceI RCATGY 2 cut(s) 351, 363
XspI CTAG 1 cut(s) 201
Zsp2I ATGCAT 2 cut(s) 349, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.