MD15G1331400.v1.1

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005024
Physical Location & Seq
Forward (+)
36760714 .. 36763975
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1331400.v1.1.491

Sequence Viewer

Length: 375 bp
ATGGTGGAAGTGTGGTGGTCCCTGTTAGCAGCTATCCCTGCAGTGGTTGCAGGACAAGCATTTCGAGTGAAGAAAAGGTGTGCTGAAGAACAGAGATTAATGAGTGCTAGGGGGAGGGAGAAGAGTTCTGATGAGATCTTTGTCTGTGAAAGGGTATGTACATCAAAGAGAATGCTGAAAAAGGTAGGTGCATTTTCAAAGGATCCAATTCCTGATAGCTGTGTTACCGTGTGCGGTGTATCTGAGCTGGATGCTTGTGCTGATGCATGTGCACGCACTGTTTGCGTAAACCAACATCAAGTACCTAACTGGAATGACATCTGCCTTCGGAGATGCCAGAGTGAATGTCTTAAACTCTCTTCTTCCAGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.71

Weight (kDa)

8.53

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 234
AclWI GGATC 2 cut(s) 197, 210
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 2 cut(s) 160, 303
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 1 cut(s) 198
AluBI AGCT 3 cut(s) 32, 219, 247
AluI AGCT 3 cut(s) 32, 219, 247
Alw21I GWGCWC 1 cut(s) 274
Alw44I GTGCAC 1 cut(s) 270
AlwI GGATC 2 cut(s) 197, 210
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AseI ATTAAT 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 18
AvaII GGWCC 1 cut(s) 18
BaeGI GKGCMC 1 cut(s) 274
BamHI GGATCC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 1 cut(s) 41
BfaI CTAG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 39
BglII AGATCT 1 cut(s) 135
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 2 cut(s) 20, 204
BmsI GCATC 3 cut(s) 241, 253, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 2 cut(s) 314, 366
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 2 cut(s) 314, 366
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 274
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 4
BsmI GAATGC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 2 cut(s) 135, 202
BspACI CCGC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 20, 204
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 2 cut(s) 197, 210
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 2 cut(s) 314, 366
BssMI GATC 2 cut(s) 135, 202
Bst4CI ACNGT 2 cut(s) 229, 280
Bst6I CTCTTC 2 cut(s) 116, 364
BstAPI GCANNNNNTGC 2 cut(s) 47, 282
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 2 cut(s) 243, 372
BstF5I GGATG 1 cut(s) 256
BstKTI GATC 2 cut(s) 138, 205
BstMBI GATC 2 cut(s) 135, 202
BstMWI GCNNNNNNNGC 4 cut(s) 38, 47, 56, 282
BstNSI RCATGY 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 2 cut(s) 135, 202
BstYI RGATCY 2 cut(s) 135, 202
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 276
Cac8I GCNNGC 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 18
Csp6I GTAC 2 cut(s) 159, 302
CviAII CATG 1 cut(s) 267
CviJI RGCY 3 cut(s) 32, 219, 247
CviKI_1 RGCY 3 cut(s) 32, 219, 247
CviQI GTAC 2 cut(s) 159, 302
DdeI CTNAG 2 cut(s) 243, 372
DpnI GATC 2 cut(s) 137, 204
DpnII GATC 2 cut(s) 135, 202
Eam1104I CTCTTC 2 cut(s) 116, 364
EarI CTCTTC 2 cut(s) 116, 364
Eco47I GGWCC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 105
EcoT22I ATGCAT 1 cut(s) 268
FaeI CATG 1 cut(s) 270
FaiI YATR 2 cut(s) 157, 268
FaqI GGGAC 1 cut(s) 4
FatI CATG 1 cut(s) 266
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 108
GluI GCNGC 1 cut(s) 30
Hin1II CATG 1 cut(s) 270
Hpy166II GTNNAC 2 cut(s) 272, 289
Hpy188I TCNGA 3 cut(s) 130, 244, 330
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 272, 289
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 2 cut(s) 229, 280
HpyCH4V TGCA 5 cut(s) 41, 50, 191, 266, 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 47, 56, 282
HpyF3I CTNAG 2 cut(s) 243, 372
Hsp92II CATG 1 cut(s) 270
Kzo9I GATC 2 cut(s) 135, 202
LpnPI CCDG 7 cut(s) 35, 36, 51, 225, 233, 295, 350
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 3 cut(s) 241, 253, 323
MaeI CTAG 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 223
MalI GATC 2 cut(s) 137, 204
MboI GATC 2 cut(s) 135, 202
MboII GAAGA 5 cut(s) 82, 98, 133, 351, 354
MflI RGATCY 2 cut(s) 135, 202
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 1 cut(s) 207
MnlI CCTC 1 cut(s) 108
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 2 cut(s) 98, 351
Mva1269I GAATGC 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 38, 47, 56, 282
NdeII GATC 2 cut(s) 135, 202
NlaIII CATG 1 cut(s) 270
NlaIV GGNNCC 2 cut(s) 20, 204
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 1 cut(s) 270
PctI GAATGC 1 cut(s) 177
PkrI GCNGC 1 cut(s) 31
PshBI ATTAAT 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 20, 204
PspPI GGNCC 1 cut(s) 18
PstI CTGCAG 1 cut(s) 43
PsuI RGATCY 2 cut(s) 135, 202
RsaI GTAC 2 cut(s) 160, 303
RsaNI GTAC 2 cut(s) 159, 302
SaqAI TTAA 2 cut(s) 98, 351
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 135, 202
Sau96I GGNCC 1 cut(s) 18
SduI GDGCHC 1 cut(s) 274
SetI ASST 7 cut(s) 34, 80, 186, 190, 221, 249, 307
SfaNI GCATC 3 cut(s) 241, 253, 323
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 18
Sse9I AATT 1 cut(s) 207
SsiI CCGC 1 cut(s) 234
SspMI CTAG 1 cut(s) 108
TaaI ACNGT 2 cut(s) 229, 280
TaqI TCGA 1 cut(s) 64
TasI AATT 1 cut(s) 207
TatI WGTACW 1 cut(s) 158
Tru1I TTAA 2 cut(s) 98, 351
Tru9I TTAA 2 cut(s) 98, 351
TscAI CASTG 2 cut(s) 48, 283
TseI GCWGC 1 cut(s) 29
TspRI CASTG 2 cut(s) 48, 283
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 18
VspI ATTAAT 1 cut(s) 98
XceI RCATGY 1 cut(s) 270
XspI CTAG 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.