Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004574

Physical Location & Seq

Genomic Coordinates Reverse (-)

26148150 .. 26150593

Gene Structure 2444 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G65430.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 747 bp

ATGGCGACGACCTTAAGCAGAGATCAATATGTCTACATGGCGAAGCTCGCCGAGCAAGCCGAGCGTTACGAAGAGATGGTTCAATTCATGGAACAGCTCGTAAGTGGAGCTACACCGGCCGGTGAGCTGACCGTAGAAGAGAGGAATCTTCTCTCGGTCGCGTATAAGAACGTGATTGGATCTCTTCGTGCGGCATGGAGAATCGTGTCTTCGATTGAGCAAAAGGAAGAGAGCAGGAAGAACGAAGAACACGTGTCGCTTGTTAAGGATTACAGATCTAAAGTTGAGACTGAGCTTTCTTCGATCTGTTCTGGGATTCTCAGGTTACTTGATTCGCATCTAATTCCTTCAGCTACTGCCAGTGAGTCTAAGGTTTTTTACCTGAAGATGAAAGGAGATTATCATCGTTATTTGGCTGAGTTTAAATCTGGTGATGAGAGGAAAACTGCTGCTGAAGATACTATGATCGCTTACAAAGCTGCTCAGGACGTTGCAGTTGCTGATCTAGCACCTACACATCCGATCAGGCTTGGTTTGGCTCTTAACTTCTCAGTGTTTTACTACGAGATTCTCAACTCTTCAGAGAAAGCTTGTAGCATGGCGAAACAGGCTTTTGAAGAAGCCATTGCTGAGCTGGACACATTGGGAGAGGAGTCATACAAGGACAGTACTCTCATCATGCAGTTGCTAAGGGACAATCTAACCCTTTGGACCTCCGATATGCAGGAGCAGATGGATGAGGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0019222 GO:0050789 GO:0050826 GO:0050896 GO:0065007

KEGG Pathways

Metabolic & Signaling

ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203

Pfam Domains

Protein Families

14-3-3

Protein Analysis

248

Amino Acids

28.03

Weight (kDa)

4.82

Isoelectric Point (pI)

45.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
14-3-3	PF00244	13 - 238	1.5e-102	14-3-3 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004574)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65430 AT5G65430 AT5G65430
fragaria_vesca	FvH4_1g19920 FvH4_5g35740
malus_domestica	MD01G1037700.v1.1 MD08G1193600.v1.1 MD15G1315300.v1.1
prunus_persica	Prupe.1G528100_v2.0.a1 Prupe.6G196400_v2.0.a1
pyrus_communis	pycom01g06560 pycom08g16670 pycom15g27850
rosa_chinensis	RchiOBHm_Chr2g0111721 RchiOBHm_Chr7g0237361
rosa_laevigata	RLG00000001009 RLG00000017920
rosa_multiflora	Rmu_sc0000448.1_g000010
rosa_roxburghii	Rroxscaffold_2G00132140 Rroxscaffold_3G00224280
rosa_rugosa	Rorug02G0175800 Rorug07G0302900
rosa_samantha	Rh2AG228700 Rh2BG241000 Rh2CG232100 Rh2DG236700 Rh7BG430000 Rh7CG477500 Rh7DG446800
rosa_wichuraiana	Rw2G017670 Rw7G038110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	33
AccII	CGCG	1 cut(s)	161
AciI	CCGC	1 cut(s)	191
AclWI	GGATC	1 cut(s)	187
AcoI	YGGCCR	1 cut(s)	117
AcuI	CTGAAG	4 cut(s)	333, 404, 474, 564
AcvI	CACGTG	1 cut(s)	253
AfaI	GTAC	1 cut(s)	670
AflII	CTTAAG	1 cut(s)	13
AflIII	ACRYGT	2 cut(s)	250, 252
AgsI	TTSAA	2 cut(s)	83, 617
AluBI	AGCT	9 cut(s)	46, 97, 110, 127, 295, 353, 479, 590, 634
AluI	AGCT	9 cut(s)	46, 97, 110, 127, 295, 353, 479, 590, 634
Alw26I	GTCTC	1 cut(s)	281
AlwI	GGATC	1 cut(s)	187
AlwNI	CAGNNNCTG	2 cut(s)	356, 500
AoxI	GGCC	2 cut(s)	117, 741
ApeKI	GCWGC	2 cut(s)	449, 479
AspS9I	GGNCC	1 cut(s)	711
AsuHPI	GGTGA	2 cut(s)	134, 443
AvaII	GGWCC	1 cut(s)	711
BbrPI	CACGTG	1 cut(s)	253
BbsI	GAAGAC	1 cut(s)	201
BbvI	GCAGC	2 cut(s)	436, 466
BccI	CCATC	2 cut(s)	70, 727
BcoDI	GTCTC	1 cut(s)	281
BfaI	CTAG	1 cut(s)	506
BfrI	CTTAAG	1 cut(s)	13
BglII	AGATCT	1 cut(s)	275
BisI	GCNGC	3 cut(s)	192, 450, 480
BlpI	GCTNAGC	1 cut(s)	630
BlsI	GCNGC	3 cut(s)	193, 451, 481
BmcAI	AGTACT	1 cut(s)	670
Bme18I	GGWCC	1 cut(s)	711
BmgT120I	GGNCC	1 cut(s)	711
BmsI	GCATC	1 cut(s)	346
BpiI	GAAGAC	1 cut(s)	201
Bpu10I	CCTNAGC	2 cut(s)	483, 689
Bpu1102I	GCTNAGC	1 cut(s)	630
BsaAI	YACGTR	1 cut(s)	253
BsaBI	GATNNNNATC	2 cut(s)	336, 402
Bse118I	RCCGGY	2 cut(s)	115, 119
Bse1I	ACTGG	1 cut(s)	360
Bse3DI	GCAATG	1 cut(s)	624
Bse8I	GATNNNNATC	2 cut(s)	336, 402
BseGI	GGATG	2 cut(s)	517, 742
BseJI	GATNNNNATC	2 cut(s)	336, 402
BseMI	GCAATG	1 cut(s)	624
BseMII	CTCAG	6 cut(s)	282, 334, 408, 497, 564, 621
BseNI	ACTGG	1 cut(s)	360
BseRI	GAGGAG	1 cut(s)	665
BseX3I	CGGCCG	1 cut(s)	117
BseXI	GCAGC	2 cut(s)	436, 466
Bsh1236I	CGCG	1 cut(s)	161
Bsh1285I	CGRYCG	2 cut(s)	120, 159
BshFI	GGCC	2 cut(s)	119, 743
BsiEI	CGRYCG	2 cut(s)	120, 159
BsiSI	CCGG	2 cut(s)	116, 120
BslFI	GGGAC	1 cut(s)	707
BsmAI	GTCTC	1 cut(s)	281
BsmFI	GGGAC	1 cut(s)	707
BsnI	GGCC	2 cut(s)	119, 743
Bsp143I	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
Bsp1720I	GCTNAGC	1 cut(s)	630
BspACI	CCGC	1 cut(s)	191
BspANI	GGCC	2 cut(s)	119, 743
BspCNI	CTCAG	6 cut(s)	283, 333, 409, 496, 563, 622
BspFNI	CGCG	1 cut(s)	161
BspPI	GGATC	1 cut(s)	187
BspTI	CTTAAG	1 cut(s)	13
BsrDI	GCAATG	1 cut(s)	624
BsrFI	RCCGGY	2 cut(s)	115, 119
BsrI	ACTGG	1 cut(s)	360
BssAI	RCCGGY	2 cut(s)	115, 119
BssMI	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
Bst4CI	ACNGT	2 cut(s)	133, 668
Bst6I	CTCTTC	5 cut(s)	66, 132, 189, 222, 583
BstAFI	CTTAAG	1 cut(s)	13
BstBAI	YACGTR	1 cut(s)	253
BstC8I	GCNNGC	2 cut(s)	48, 57
BstDEI	CTNAG	8 cut(s)	291, 320, 369, 417, 483, 550, 630, 689
BstF5I	GGATG	2 cut(s)	517, 742
BstFNI	CGCG	1 cut(s)	161
BstKTI	GATC	7 cut(s)	25, 182, 278, 306, 468, 505, 525
BstMAI	GTCTC	1 cut(s)	281
BstMBI	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
BstMCI	CGRYCG	2 cut(s)	120, 159
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 52, 56, 61, 116, 476, 506, 608
BstUI	CGCG	1 cut(s)	161
BstV1I	GCAGC	2 cut(s)	436, 466
BstV2I	GAAGAC	1 cut(s)	201
BstX2I	RGATCY	2 cut(s)	179, 275
BstYI	RGATCY	2 cut(s)	179, 275
BstZI	CGGCCG	1 cut(s)	117
BsuRI	GGCC	2 cut(s)	119, 743
BtsCI	GGATG	2 cut(s)	517, 742
BtsIMutI	CAGTG	2 cut(s)	367, 558
Cac8I	GCNNGC	2 cut(s)	48, 57
CaiI	CAGNNNCTG	2 cut(s)	356, 500
Cfr10I	RCCGGY	2 cut(s)	115, 119
Cfr13I	GGNCC	1 cut(s)	711
Csp6I	GTAC	1 cut(s)	669
CviAII	CATG	5 cut(s)	37, 88, 195, 598, 679
CviQI	GTAC	1 cut(s)	669
DdeI	CTNAG	8 cut(s)	291, 320, 369, 417, 483, 550, 630, 689
DpnI	GATC	7 cut(s)	24, 181, 277, 305, 467, 504, 524
DpnII	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
DraI	TTTAAA	1 cut(s)	424
EaeI	YGGCCR	1 cut(s)	117
EagI	CGGCCG	1 cut(s)	117
Eam1104I	CTCTTC	5 cut(s)	66, 132, 189, 222, 583
EarI	CTCTTC	5 cut(s)	66, 132, 189, 222, 583
EclXI	CGGCCG	1 cut(s)	117
Eco147I	AGGCCT	1 cut(s)	743
Eco47I	GGWCC	1 cut(s)	711
Eco52I	CGGCCG	1 cut(s)	117
Eco57I	CTGAAG	4 cut(s)	333, 404, 474, 564
Eco72I	CACGTG	1 cut(s)	253
FaeI	CATG	5 cut(s)	40, 91, 198, 601, 682
FaqI	GGGAC	1 cut(s)	707
FatI	CATG	5 cut(s)	36, 87, 194, 597, 678
FblI	GTMKAC	1 cut(s)	33
Fnu4HI	GCNGC	3 cut(s)	192, 450, 480
FokI	GGATG	1 cut(s)	504
Fsp4HI	GCNGC	3 cut(s)	192, 450, 480
FspBI	CTAG	1 cut(s)	506
GluI	GCNGC	3 cut(s)	192, 450, 480
HaeIII	GGCC	2 cut(s)	119, 743
HapII	CCGG	2 cut(s)	116, 120
Hin1II	CATG	5 cut(s)	40, 91, 198, 601, 682
HindIII	AAGCTT	1 cut(s)	588
HinfI	GANTC	7 cut(s)	145, 201, 316, 332, 365, 568, 653
HpaII	CCGG	2 cut(s)	116, 120
HphI	GGTGA	2 cut(s)	134, 443
Hpy166II	GTNNAC	1 cut(s)	34
Hpy188I	TCNGA	3 cut(s)	522, 583, 718
Hpy188III	TCNNGA	1 cut(s)	485
Hpy8I	GTNNAC	1 cut(s)	34
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	1 cut(s)	357
HpyCH4III	ACNGT	2 cut(s)	133, 668
HpyCH4IV	ACGT	3 cut(s)	171, 252, 489
HpyCH4V	TGCA	3 cut(s)	494, 682, 724
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 52, 56, 61, 116, 476, 506, 608
HpyF3I	CTNAG	8 cut(s)	291, 320, 369, 417, 483, 550, 630, 689
HpySE526I	ACGT	3 cut(s)	171, 252, 489
Hsp92II	CATG	5 cut(s)	40, 91, 198, 601, 682
Kzo9I	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
LmnI	GCTCC	2 cut(s)	107, 727
Lsp1109I	GCAGC	2 cut(s)	436, 466
LweI	GCATC	1 cut(s)	346
MaeI	CTAG	1 cut(s)	506
MaeII	ACGT	3 cut(s)	171, 252, 489
MaeIII	GTNAC	2 cut(s)	65, 324
MalI	GATC	7 cut(s)	24, 181, 277, 305, 467, 504, 524
MboI	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
MflI	RGATCY	2 cut(s)	179, 275
MluCI	AATT	2 cut(s)	83, 342
MlyI	GAGTC	2 cut(s)	374, 662
MnlI	CCTC	5 cut(s)	135, 432, 643, 724, 733
MseI	TTAA	4 cut(s)	14, 264, 423, 543
MspCI	CTTAAG	1 cut(s)	13
MspI	CCGG	2 cut(s)	116, 120
MvnI	CGCG	1 cut(s)	161
MwoI	GCNNNNNNNGC	8 cut(s)	47, 52, 56, 61, 116, 476, 506, 608
NdeII	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
NlaIII	CATG	5 cut(s)	40, 91, 198, 601, 682
NmeAIII	GCCGAG	2 cut(s)	76, 85
PceI	AGGCCT	1 cut(s)	743
PcsI	WCGNNNNNNNCGW	1 cut(s)	249
PfeI	GAWTC	5 cut(s)	145, 201, 316, 332, 568
PkrI	GCNGC	3 cut(s)	193, 451, 481
PleI	GAGTC	2 cut(s)	373, 661
PmaCI	CACGTG	1 cut(s)	253
PmlI	CACGTG	1 cut(s)	253
PpsI	GAGTC	2 cut(s)	373, 661
Ppu21I	YACGTR	1 cut(s)	253
PspCI	CACGTG	1 cut(s)	253
PspPI	GGNCC	1 cut(s)	711
PstNI	CAGNNNCTG	2 cut(s)	356, 500
PsuI	RGATCY	2 cut(s)	179, 275
RsaI	GTAC	1 cut(s)	670
RsaNI	GTAC	1 cut(s)	669
SaqAI	TTAA	4 cut(s)	14, 264, 423, 543
SatI	GCNGC	3 cut(s)	192, 450, 480
Sau3AI	GATC	7 cut(s)	22, 179, 275, 303, 465, 502, 522
Sau96I	GGNCC	1 cut(s)	711
ScaI	AGTACT	1 cut(s)	670
SchI	GAGTC	2 cut(s)	374, 662
SfaNI	GCATC	1 cut(s)	346
SinI	GGWCC	1 cut(s)	711
SmlI	CTYRAG	1 cut(s)	13
SmoI	CTYRAG	1 cut(s)	13
Sse9I	AATT	2 cut(s)	83, 342
SseBI	AGGCCT	1 cut(s)	743
SsiI	CCGC	1 cut(s)	191
SspMI	CTAG	1 cut(s)	506
StuI	AGGCCT	1 cut(s)	743
TaaI	ACNGT	2 cut(s)	133, 668
TaiI	ACGT	3 cut(s)	174, 255, 492
TaqI	TCGA	2 cut(s)	212, 302
TaqII	GACCGA	1 cut(s)	145
TasI	AATT	2 cut(s)	83, 342
TatI	WGTACW	1 cut(s)	668
TauI	GCSGC	1 cut(s)	194
TfiI	GAWTC	5 cut(s)	145, 201, 316, 332, 568
Tru1I	TTAA	4 cut(s)	14, 264, 423, 543
Tru9I	TTAA	4 cut(s)	14, 264, 423, 543
TscAI	CASTG	2 cut(s)	367, 558
TseI	GCWGC	2 cut(s)	449, 479
TspDTI	ATGAA	2 cut(s)	76, 404
TspRI	CASTG	2 cut(s)	367, 558
Vha464I	CTTAAG	1 cut(s)	13
VpaK11BI	GGWCC	1 cut(s)	711
XcmI	CCANNNNNNNNNTGG	1 cut(s)	631
XmiI	GTMKAC	1 cut(s)	33
XspI	CTAG	1 cut(s)	506
ZrmI	AGTACT	1 cut(s)	670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.