MD08G1193600.v1.1

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004574
Physical Location & Seq
Reverse (-)
24908573 .. 24911343
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1193600.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGCTTCCGTCGTCCCCGATAACCTCACCAGGGAGCAGTACGTGTACCTGGCGAAGCTCTCCGAGCAAGCGGAGCGCTACGAGGAGATGGTCCAGTTCATGGAGAAGCTGGTCGTTGGTTCCACTCCGGCCGCCGAGCTGACCGTTGAGGAGCGCAATCTTCTCTCCGTTGCGTACAAGAACGTGATCGGCTCGCTCCGTGCCGCCTGGCGCATCGTGTCGTCGATCGAGCAGAAGGAGGAGGGCCGGAAGAACGAGGAACACGTGGCGCTCGTCAAGGAGTACAGATCCAAGGTCGAGTCCGAGCTCTCTGATGTCTGCGCTAAGATATTGAAGCTCTTGGACTCCAATCTCGTGCCGTCGGCGTCGGCCAGTGAGTCCAAGGTCTTCTATCTGAAGATGAAGGGCGACTACCACCGCTACATGGCGGAGTTCAAATCCGCCGAGGAGAGAAAGACTGCTGCTGAGGACACTATGCTTGCTTACAAGGCTGCCCAGGACATAGCGCTTGCGGATCTGTCACCAACTCATCCAATAAGATTGGGTCTGGCACTGAATTTCTCAGTGTTCTACTATGAGATTCTCAATCAGTCTGATAAAGCTTGCAGCATGGCCAAACAGGCATTTGAGGAAGCTATTGCTGAGCTAGACACTCTGGGAGAAGAATCATACAAGGACAGCACTCTAATCATGCAACTTCTAAGGGACAACCTCACTCTTTGGACTTCTGATGCTTCGGACCAGTTGGACGAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

253

Amino Acids

28.43

Weight (kDa)

4.8

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 242 6e-100 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 7 cut(s) 71, 132, 204, 418, 428, 441, 512
AclWI GGATC 2 cut(s) 282, 522
AcoI YGGCCR 3 cut(s) 129, 369, 612
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 416
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 4 cut(s) 41, 47, 176, 284
AfeI AGCGCT 2 cut(s) 77, 507
AfiI CCNNNNNNNGG 3 cut(s) 31, 100, 424
AflIII ACRYGT 2 cut(s) 42, 262
AgsI TTSAA 2 cut(s) 334, 436
AjnI CCWGG 4 cut(s) 29, 48, 206, 495
AluBI AGCT 8 cut(s) 58, 109, 139, 307, 337, 602, 635, 646
AluI AGCT 8 cut(s) 58, 109, 139, 307, 337, 602, 635, 646
Alw21I GWGCWC 1 cut(s) 309
AlwI GGATC 2 cut(s) 282, 522
Aor51HI AGCGCT 2 cut(s) 77, 507
AoxI GGCC 4 cut(s) 129, 244, 369, 612
ApeKI GCWGC 3 cut(s) 461, 491, 606
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 6 cut(s) 78, 156, 213, 271, 323, 508
AspS9I GGNCC 3 cut(s) 91, 244, 739
AsuHPI GGTGA 2 cut(s) 19, 513
AvaII GGWCC 2 cut(s) 91, 739
BalI TGGCCA 1 cut(s) 614
BanII GRGCYC 1 cut(s) 309
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 353
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 448, 478, 618
BccI CCATC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 4 cut(s) 31, 50, 208, 497
BfaI CTAG 1 cut(s) 647
BfoI RGCGCY 3 cut(s) 79, 272, 509
BglI GCCNNNNNGGC 1 cut(s) 620
BisI GCNGC 5 cut(s) 132, 204, 462, 492, 607
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 5 cut(s) 133, 205, 463, 493, 608
Bme1390I CCNGG 4 cut(s) 31, 50, 208, 497
Bme18I GGWCC 2 cut(s) 91, 739
BmgT120I GGNCC 3 cut(s) 91, 244, 739
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 4 cut(s) 31, 50, 208, 497
BmsI GCATC 2 cut(s) 222, 721
BpiI GAAGAC 1 cut(s) 379
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 642
BsaAI YACGTR 2 cut(s) 43, 265
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 5 cut(s) 30, 291, 381, 444, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 100, 424
Bse1I ACTGG 3 cut(s) 94, 372, 742
BseBI CCWGG 4 cut(s) 31, 50, 208, 497
BseDI CCNNGG 5 cut(s) 30, 291, 381, 444, 495
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 3 cut(s) 31, 100, 424
BseMII CTCAG 3 cut(s) 456, 576, 633
BseNI ACTGG 3 cut(s) 94, 372, 742
BseRI GAGGAG 4 cut(s) 98, 164, 254, 461
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 448, 478, 618
Bsh1285I CGRYCG 2 cut(s) 132, 228
BshFI GGCC 4 cut(s) 131, 246, 371, 614
BsiEI CGRYCG 2 cut(s) 132, 228
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 128, 247
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 3 cut(s) 31, 100, 424
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 4 cut(s) 131, 246, 371, 614
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 4 cut(s) 186, 225, 287, 514
Bsp1720I GCTNAGC 1 cut(s) 642
BspACI CCGC 7 cut(s) 71, 132, 204, 418, 428, 441, 512
BspANI GGCC 4 cut(s) 131, 246, 371, 614
BspCNI CTCAG 3 cut(s) 457, 575, 634
BspLI GGNNCC 1 cut(s) 121
BspPI GGATC 2 cut(s) 282, 522
BsrI ACTGG 3 cut(s) 94, 372, 742
BssECI CCNNGG 5 cut(s) 30, 291, 381, 444, 495
BssMI GATC 4 cut(s) 186, 225, 287, 514
BssNI GRCGYC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 2 cut(s) 291, 381
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 4 cut(s) 31, 50, 208, 497
Bst4CI ACNGT 1 cut(s) 145
BstACI GRCGYC 1 cut(s) 365
BstBAI YACGTR 2 cut(s) 43, 265
BstC8I GCNNGC 5 cut(s) 69, 194, 480, 510, 604
BstDEI CTNAG 5 cut(s) 324, 465, 562, 642, 701
BstF5I GGATG 1 cut(s) 529
BstH2I RGCGCY 3 cut(s) 79, 272, 509
BstHHI GCGC 6 cut(s) 78, 156, 213, 271, 323, 508
BstKTI GATC 4 cut(s) 189, 228, 290, 517
BstMBI GATC 4 cut(s) 186, 225, 287, 514
BstMCI CGRYCG 2 cut(s) 132, 228
BstMWI GCNNNNNNNGC 4 cut(s) 64, 73, 488, 620
BstNI CCWGG 4 cut(s) 31, 50, 208, 497
BstSCI CCNGG 4 cut(s) 29, 48, 206, 495
BstV1I GCAGC 3 cut(s) 448, 478, 618
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 287, 514
BstYI RGATCY 2 cut(s) 287, 514
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 4 cut(s) 131, 246, 371, 614
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 3 cut(s) 379, 551, 570
Cac8I GCNNGC 5 cut(s) 69, 194, 480, 510, 604
CfoI GCGC 6 cut(s) 78, 156, 213, 271, 323, 508
Cfr13I GGNCC 3 cut(s) 91, 244, 739
CseI GACGC 1 cut(s) 354
Csp6I GTAC 4 cut(s) 40, 46, 175, 283
CviAII CATG 4 cut(s) 100, 424, 610, 691
CviQI GTAC 4 cut(s) 40, 46, 175, 283
DdeI CTNAG 5 cut(s) 324, 465, 562, 642, 701
DpnI GATC 4 cut(s) 188, 227, 289, 516
DpnII GATC 4 cut(s) 186, 225, 287, 514
EaeI YGGCCR 3 cut(s) 129, 369, 612
EagI CGGCCG 1 cut(s) 129
EciI GGCGGA 2 cut(s) 430, 443
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 291, 381
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 91, 739
Eco47III AGCGCT 2 cut(s) 77, 507
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 416
Eco72I CACGTG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 307
EcoRII CCWGG 4 cut(s) 29, 48, 206, 495
EcoT14I CCWWGG 2 cut(s) 291, 381
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 2 cut(s) 291, 381
FaeI CATG 4 cut(s) 103, 427, 613, 694
FaiI YATR 9 cut(s) 101, 425, 476, 503, 576, 611, 670, 692, 757
FaqI GGGAC 1 cut(s) 719
FatI CATG 4 cut(s) 99, 423, 609, 690
Fnu4HI GCNGC 5 cut(s) 132, 204, 462, 492, 607
FokI GGATG 1 cut(s) 516
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 132, 204, 462, 492, 607
FspBI CTAG 1 cut(s) 647
GlaI GCGC 6 cut(s) 77, 155, 212, 270, 322, 507
GluI GCNGC 5 cut(s) 132, 204, 462, 492, 607
HaeII RGCGCY 3 cut(s) 79, 272, 509
HaeIII GGCC 4 cut(s) 131, 246, 371, 614
HapII CCGG 2 cut(s) 128, 247
HgaI GACGC 1 cut(s) 354
HhaI GCGC 6 cut(s) 78, 156, 213, 271, 323, 508
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 4 cut(s) 103, 427, 613, 694
Hin6I GCGC 6 cut(s) 76, 154, 211, 269, 321, 506
HinP1I GCGC 6 cut(s) 76, 154, 211, 269, 321, 506
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 5 cut(s) 299, 344, 377, 580, 665
HpaII CCGG 2 cut(s) 128, 247
HphI GGTGA 2 cut(s) 19, 513
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 7 cut(s) 64, 304, 313, 396, 595, 730, 739
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 4 cut(s) 14, 226, 364, 370
HpyAV CCTTC 2 cut(s) 229, 397
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 3 cut(s) 42, 183, 264
HpyCH4V TGCA 2 cut(s) 606, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 73, 488, 620
HpyF3I CTNAG 5 cut(s) 324, 465, 562, 642, 701
HpySE526I ACGT 3 cut(s) 42, 183, 264
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 4 cut(s) 103, 427, 613, 694
HspAI GCGC 6 cut(s) 76, 154, 211, 269, 321, 506
Kzo9I GATC 4 cut(s) 186, 225, 287, 514
LmnI GCTCC 4 cut(s) 34, 73, 151, 201
Lsp1109I GCAGC 3 cut(s) 448, 478, 618
LweI GCATC 2 cut(s) 222, 721
MaeI CTAG 1 cut(s) 647
MaeII ACGT 3 cut(s) 42, 183, 264
MaeIII GTNAC 1 cut(s) 519
MalI GATC 4 cut(s) 188, 227, 289, 516
MboI GATC 4 cut(s) 186, 225, 287, 514
MboII GAAGA 5 cut(s) 152, 262, 379, 409, 674
MflI RGATCY 2 cut(s) 287, 514
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 1 cut(s) 556
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 3 cut(s) 308, 338, 386
MmeI TCCRAC 1 cut(s) 726
Mox20I TGGCCA 1 cut(s) 614
MscI TGGCCA 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 614
MspI CCGG 2 cut(s) 128, 247
MspR9I CCNGG 4 cut(s) 31, 50, 208, 497
MvaI CCWGG 4 cut(s) 31, 50, 208, 497
MwoI GCNNNNNNNGC 4 cut(s) 64, 73, 488, 620
NdeII GATC 4 cut(s) 186, 225, 287, 514
NlaIII CATG 4 cut(s) 103, 427, 613, 694
NlaIV GGNNCC 1 cut(s) 121
NmeAIII GCCGAG 2 cut(s) 160, 469
NmuCI GTSAC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 270
PfeI GAWTC 2 cut(s) 580, 665
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 5 cut(s) 133, 205, 463, 493, 608
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 3 cut(s) 307, 338, 385
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 3 cut(s) 307, 338, 385
Ppu21I YACGTR 2 cut(s) 43, 265
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 4 cut(s) 29, 48, 206, 495
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 4 cut(s) 29, 48, 206, 495
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 3 cut(s) 91, 244, 739
PsuI RGATCY 2 cut(s) 287, 514
PvuI CGATCG 1 cut(s) 228
RsaI GTAC 4 cut(s) 41, 47, 176, 284
RsaNI GTAC 4 cut(s) 40, 46, 175, 283
SacI GAGCTC 1 cut(s) 309
SatI GCNGC 5 cut(s) 132, 204, 462, 492, 607
Sau3AI GATC 4 cut(s) 186, 225, 287, 514
Sau96I GGNCC 3 cut(s) 91, 244, 739
SchI GAGTC 3 cut(s) 308, 338, 386
ScrFI CCNGG 4 cut(s) 31, 50, 208, 497
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 2 cut(s) 222, 721
SinI GGWCC 2 cut(s) 91, 739
Sse9I AATT 1 cut(s) 556
SsiI CCGC 7 cut(s) 71, 132, 204, 418, 428, 441, 512
SspMI CTAG 1 cut(s) 647
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 4 cut(s) 29, 48, 206, 495
StyI CCWWGG 2 cut(s) 291, 381
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 3 cut(s) 45, 186, 267
TaqI TCGA 3 cut(s) 224, 228, 297
TasI AATT 1 cut(s) 556
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 134, 206
TfiI GAWTC 2 cut(s) 580, 665
TscAI CASTG 3 cut(s) 379, 558, 570
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 3 cut(s) 461, 491, 606
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 88, 416
TspGWI ACGGA 2 cut(s) 157, 188
TspRI CASTG 3 cut(s) 379, 558, 570
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 91, 739
XapI RAATTY 1 cut(s) 556
XspI CTAG 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.