MD01G1037700.v1.1

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004574
Physical Location & Seq
Reverse (-)
13038612 .. 13047589
8978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1037700.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGCTTCCGCCGTCCCAGAAAACCTCAGCCGCGAACAGTACGTGTACCTGGCGAAGCTCGCCGAGCAGGCCGAGCGCTACGAGGAGATGGTGAGCTTCATGGAGAAGCTGGTGGTGGGCTCCACCGCCGCCGGGACCGAGCTGACGGTGGAGGAGCGCAATCTGCTCAGCGTCGCCTACAAGAACGTCATCGGCTCGCTCCGCGCCGCCTGGCGCATAATCTCGTCCATCGAGCAGAAGGAGGAAGGCCGTCGCAACGAGGAGCACGTGGCGCTCGTCAAGCAGTACAGATCCAAGGTGGAGACTGAGCTCTCCGCCATCTGCGCTGGAATCCTGAAGCTCCTGCAATCGCACCTTGTTCCTTCGGCGACCACCGGAGAGTCCAAGGTCTTCTACTTGAAGATGAAGGGCGATTATCATCGATACCTCGCCGAGTTCAAAAATGGCGACGAGAGGAAGACCGCCGCCGAGGACACCATGGTTGCATACAAGGCTGCTCAGGATATTGCGGTGAGTGATTTAGCGCCCACGCATCCAATAAGGTTGGGGCTGGCGCTCAACTTTTCGGTGTTTTACTACGAGATCCTCAACTCCTCGGAGAAAGCTTGTAGCATGGCTAAACAGGCTTTTGAGGAAGCTATTGCTGAACTTGATACACTGGGAGAGGAATCATACAAGGATAGCACTTTGATTATGCAACTGCTGAGGGACAACCTCACTCTTTGGACTTCAGATTTGCAGGTCAGTGCTACTTTTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

254

Amino Acids

28.41

Weight (kDa)

5.22

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 243 1e-101 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 730
AccII CGCG 2 cut(s) 33, 204
AclWI GGATC 2 cut(s) 285, 577
AcuI CTGAAG 2 cut(s) 356, 714
AcvI CACGTG 1 cut(s) 268
AfaI GTAC 3 cut(s) 41, 47, 287
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 132
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 2 cut(s) 400, 439
AjnI CCWGG 2 cut(s) 48, 209
AluBI AGCT 8 cut(s) 58, 96, 109, 142, 310, 340, 605, 638
AluI AGCT 8 cut(s) 58, 96, 109, 142, 310, 340, 605, 638
Alw21I GWGCWC 2 cut(s) 267, 312
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 2 cut(s) 285, 577
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 2 cut(s) 69, 247
ApeKI GCWGC 1 cut(s) 494
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
AspLEI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 526, 556
AspS9I GGNCC 1 cut(s) 135
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 103, 523
AvaII GGWCC 1 cut(s) 135
BanII GRGCYC 2 cut(s) 122, 312
BbrPI CACGTG 1 cut(s) 268
BbsI GAAGAC 2 cut(s) 382, 464
Bbv12I GWGCWC 2 cut(s) 267, 312
BbvCI CCTCAGC 2 cut(s) 26, 704
BbvI GCAGC 1 cut(s) 481
BccI CCATC 3 cut(s) 82, 236, 326
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 2 cut(s) 50, 211
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 296
BfoI RGCGCY 4 cut(s) 79, 275, 527, 557
BfuAI ACCTGC 1 cut(s) 730
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 5 cut(s) 31, 129, 207, 465, 495
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 5 cut(s) 32, 130, 208, 466, 496
Bme1390I CCNGG 3 cut(s) 50, 133, 211
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 2 cut(s) 121, 136
BmrFI CCNGG 3 cut(s) 50, 133, 211
BmrI ACTGGG 1 cut(s) 668
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 382, 464
Bpu10I CCTNAGC 3 cut(s) 26, 498, 704
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 421
BsaAI YACGTR 2 cut(s) 43, 268
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 2 cut(s) 50, 211
BseCI ATCGAT 1 cut(s) 421
BseDI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMII CTCAG 5 cut(s) 40, 181, 297, 512, 695
BseNI ACTGG 1 cut(s) 663
BseRI GAGGAG 4 cut(s) 98, 167, 275, 583
BseXI GCAGC 1 cut(s) 481
Bsh1236I CGCG 2 cut(s) 33, 204
BshFI GGCC 2 cut(s) 71, 249
BshVI ATCGAT 1 cut(s) 421
BsiHKAI GWGCWC 2 cut(s) 267, 312
BsiSI CCGG 2 cut(s) 132, 375
BslFI GGGAC 2 cut(s) 148, 722
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 2 cut(s) 148, 722
BsnI GGCC 2 cut(s) 71, 249
Bsp1286I GDGCHC 3 cut(s) 122, 267, 312
Bsp143I GATC 2 cut(s) 290, 582
Bsp1720I GCTNAGC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 477
BspANI GGCC 2 cut(s) 71, 249
BspCNI CTCAG 5 cut(s) 39, 180, 298, 511, 696
BspDI ATCGAT 1 cut(s) 421
BspFNI CGCG 2 cut(s) 33, 204
BspLI GGNNCC 2 cut(s) 121, 136
BspMI ACCTGC 1 cut(s) 730
BspPI GGATC 2 cut(s) 285, 577
BsrI ACTGG 1 cut(s) 663
BssECI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BssMI GATC 2 cut(s) 290, 582
BssT1I CCWWGG 3 cut(s) 294, 384, 477
Bst2UI CCWGG 2 cut(s) 50, 211
Bst4CI ACNGT 2 cut(s) 39, 148
BstBAI YACGTR 2 cut(s) 43, 268
BstC8I GCNNGC 4 cut(s) 60, 69, 197, 552
BstDEI CTNAG 5 cut(s) 26, 167, 306, 498, 704
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 2 cut(s) 33, 204
BstH2I RGCGCY 4 cut(s) 79, 275, 527, 557
BstHHI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 526, 556
BstKTI GATC 2 cut(s) 293, 585
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 2 cut(s) 290, 582
BstNI CCWGG 2 cut(s) 50, 211
BstSCI CCNGG 3 cut(s) 48, 131, 209
BstUI CGCG 2 cut(s) 33, 204
BstV1I GCAGC 1 cut(s) 481
BstV2I GAAGAC 2 cut(s) 382, 464
BstX2I RGATCY 2 cut(s) 290, 582
BstYI RGATCY 2 cut(s) 290, 582
Bsu15I ATCGAT 1 cut(s) 421
BsuRI GGCC 2 cut(s) 71, 249
BsuTUI ATCGAT 1 cut(s) 421
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 2 cut(s) 656, 751
BveI ACCTGC 1 cut(s) 730
Cac8I GCNNGC 4 cut(s) 60, 69, 197, 552
CfoI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 526, 556
Cfr13I GGNCC 1 cut(s) 135
ClaI ATCGAT 1 cut(s) 421
CseI GACGC 1 cut(s) 160
Csp6I GTAC 3 cut(s) 40, 46, 286
CviAII CATG 3 cut(s) 100, 478, 613
CviQI GTAC 3 cut(s) 40, 46, 286
DdeI CTNAG 5 cut(s) 26, 167, 306, 498, 704
DpnI GATC 2 cut(s) 292, 584
DpnII GATC 2 cut(s) 290, 582
EciI GGCGGA 1 cut(s) 304
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 3 cut(s) 294, 384, 477
Eco24I GRGCYC 2 cut(s) 122, 312
Eco47I GGWCC 1 cut(s) 135
Eco47III AGCGCT 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 356, 714
Eco72I CACGTG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 48, 209
EcoT14I CCWWGG 3 cut(s) 294, 384, 477
EcoT38I GRGCYC 2 cut(s) 122, 312
ErhI CCWWGG 3 cut(s) 294, 384, 477
FaeI CATG 3 cut(s) 103, 481, 616
FaiI YATR 7 cut(s) 101, 218, 479, 487, 614, 673, 695
FaqI GGGAC 2 cut(s) 148, 722
FatI CATG 3 cut(s) 99, 477, 612
Fnu4HI GCNGC 5 cut(s) 31, 129, 207, 465, 495
FokI GGATG 1 cut(s) 519
FriOI GRGCYC 2 cut(s) 122, 312
Fsp4HI GCNGC 5 cut(s) 31, 129, 207, 465, 495
GlaI GCGC 8 cut(s) 77, 158, 205, 215, 273, 325, 525, 555
GluI GCNGC 5 cut(s) 31, 129, 207, 465, 495
HaeII RGCGCY 4 cut(s) 79, 275, 527, 557
HaeIII GGCC 2 cut(s) 71, 249
HapII CCGG 2 cut(s) 132, 375
HgaI GACGC 1 cut(s) 160
HhaI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 526, 556
Hin1II CATG 3 cut(s) 103, 481, 616
Hin6I GCGC 8 cut(s) 76, 157, 204, 214, 272, 324, 524, 554
HinP1I GCGC 8 cut(s) 76, 157, 204, 214, 272, 324, 524, 554
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 3 cut(s) 330, 380, 668
HpaII CCGG 2 cut(s) 132, 375
HphI GGTGA 2 cut(s) 103, 523
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 598, 733
Hpy188III TCNNGA 2 cut(s) 334, 500
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 3 cut(s) 176, 255, 452
HpyAV CCTTC 4 cut(s) 232, 239, 372, 400
HpyCH4III ACNGT 2 cut(s) 39, 148
HpyCH4IV ACGT 3 cut(s) 42, 186, 267
HpyCH4V TGCA 4 cut(s) 346, 485, 697, 739
HpyF3I CTNAG 5 cut(s) 26, 167, 306, 498, 704
HpySE526I ACGT 3 cut(s) 42, 186, 267
Hsp92II CATG 3 cut(s) 103, 481, 616
HspAI GCGC 8 cut(s) 76, 157, 204, 214, 272, 324, 524, 554
Kzo9I GATC 2 cut(s) 290, 582
LmnI GCTCC 5 cut(s) 125, 154, 204, 262, 345
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 1 cut(s) 541
MaeII ACGT 3 cut(s) 42, 186, 267
MalI GATC 2 cut(s) 292, 584
MboI GATC 2 cut(s) 290, 582
MboII GAAGA 3 cut(s) 382, 412, 469
MflI RGATCY 2 cut(s) 290, 582
MhlI GDGCHC 3 cut(s) 122, 267, 312
MlyI GAGTC 1 cut(s) 389
MspI CCGG 2 cut(s) 132, 375
MspR9I CCNGG 3 cut(s) 50, 133, 211
MvaI CCWGG 2 cut(s) 50, 211
MvnI CGCG 2 cut(s) 33, 204
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 477
NdeII GATC 2 cut(s) 290, 582
NlaIII CATG 3 cut(s) 103, 481, 616
NlaIV GGNNCC 2 cut(s) 121, 136
NmeAIII GCCGAG 4 cut(s) 88, 97, 457, 493
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 273
PfeI GAWTC 2 cut(s) 330, 668
PkrI GCNGC 5 cut(s) 32, 130, 208, 466, 496
PleI GAGTC 1 cut(s) 388
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 2 cut(s) 43, 268
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 48, 209
PspCI CACGTG 1 cut(s) 268
PspGI CCWGG 2 cut(s) 48, 209
PspN4I GGNNCC 2 cut(s) 121, 136
PspPI GGNCC 1 cut(s) 135
PsuI RGATCY 2 cut(s) 290, 582
RsaI GTAC 3 cut(s) 41, 47, 287
RsaNI GTAC 3 cut(s) 40, 46, 286
SacI GAGCTC 1 cut(s) 312
SatI GCNGC 5 cut(s) 31, 129, 207, 465, 495
Sau3AI GATC 2 cut(s) 290, 582
Sau96I GGNCC 1 cut(s) 135
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 3 cut(s) 50, 133, 211
SduI GDGCHC 3 cut(s) 122, 267, 312
SfaNI GCATC 1 cut(s) 541
SinI GGWCC 1 cut(s) 135
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 3 cut(s) 48, 131, 209
StyI CCWWGG 3 cut(s) 294, 384, 477
TaaI ACNGT 2 cut(s) 39, 148
TaiI ACGT 3 cut(s) 45, 189, 270
TaqI TCGA 2 cut(s) 231, 421
TaqII GACCGA 1 cut(s) 152
TatI WGTACW 1 cut(s) 285
TauI GCSGC 4 cut(s) 33, 131, 209, 467
TfiI GAWTC 2 cut(s) 330, 668
TscAI CASTG 2 cut(s) 663, 751
TseI GCWGC 1 cut(s) 494
TspDTI ATGAA 2 cut(s) 88, 419
TspRI CASTG 2 cut(s) 663, 751
VpaK11BI GGWCC 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.