Rorug02G0175800

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004574
Physical Location & Seq
Reverse (-)
16154073 .. 16156172
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0175800.1

Sequence Viewer

Length: 1155 bp
ATGACTTCTCCTGTCGAATTCAAAACCCAGAATTATTTGGTTTTGAAACCAAACAAGGCTGGTCCGATCAAGCTCGTACAATTCTTGTGGGCTGGCTACATAGCGGAGGGAGAAGATGCTATGATATTGTGCCCAAAGGGAGAGGAGGAAAATCTCAAACTTTTTGAGTGGTTCCTCAACCTTCCCTCTAGTAAGCGAAACTGGCTTGAGCTCTCTCTTGATCTTTTAACAGGTGACATAAAGAAAGAGAAAGACTCAGCGAGTTTCATTTCCACTGTTGGACATACAGACAAGAGAGTGAAGTTGGATAGTAGCATCAGCCGGTTAGATGTCAGATATAGAAGAGCACTCTCTGCAATGGCTGCTAAAATAGCATATGAGAACCAAGCCTTCATTGAAACTACTGTAAAAAATCATTGGAAGGAAATTAATGGTCCTGAAAAGATTACCCAAAACAAGAAGATGAAACTAATCAAATTCTTTGACTTTTATAATTATTATCAAGGAAGACATACTACTCAAGCCTTCATGCTCCATGACCCTGTTGATCAATTGATCGTTGTGGCATTCAGAGGTACCGAGTTCTTTGATGCAGATGCATGGAGTGAGTTCTTTGATGCAGATGCATGGAGTACTGACCTCGATATTTCCTGGTACGACTACAGGAATCAATTGCGAGATCTCGTGAAAGACACCAATAACACGAAATTCATAGTGACCGGCCATAGCTTGGGTGGTGCACTGGCAATTTTGTTTGCAGCAATTTTGGCATTGCATAAGGAAGATTTGTTGTTGGAAAGATTGCAAGGGGTTTATACGTTCGGGCAGCCACGAGTTGGGGATAAGGACTTTCAAGGATTCATGGAGAAACAAATGAAAAATCATAAGATCAAGTATCAGAGGATCGTTTATAGTAACGATTTGGTTCCTAGGATACCCTTTGATAGTTCTACATTTTTGTTCAAGCATTTTGGGACCTGCCTCTACTATAACAGCCTGTATAAAGAAAAGATACTTACAGAAGAGCCAAACAAGAATTACTTTGCTCCAAAGGAGGCAATAATTAGTTGCTGTTTGGGAGCTCATAAGAAGCTTCATAATTCCACACGTAGAAGGATGGGAGTACAAAGAAACTTGGTTACTCAAGGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

384

Amino Acids

44.5

Weight (kDa)

8.85

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phospholipase-like PF26363 118 - 253 7e-07 Tle-like phospholipase domain
Lipase_3 PF01764 217 - 315 4.3e-27 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
Acc36I ACCTGC 1 cut(s) 986
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 575
AccB7I CCANNNNNTGG 2 cut(s) 730, 836
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 911
AcoI YGGCCR 1 cut(s) 721
AcsI RAATTY 3 cut(s) 17, 476, 707
AfaI GTAC 5 cut(s) 78, 577, 634, 656, 1125
AfiI CCNNNNNNNGG 2 cut(s) 730, 836
AflIII ACRYGT 1 cut(s) 1106
AgsI TTSAA 5 cut(s) 22, 46, 398, 854, 964
AjnI CCWGG 1 cut(s) 650
AluBI AGCT 5 cut(s) 73, 211, 729, 1082, 1093
AluI AGCT 5 cut(s) 73, 211, 729, 1082, 1093
Alw21I GWGCWC 4 cut(s) 213, 349, 742, 1084
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 1 cut(s) 911
AoxI GGCC 1 cut(s) 721
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 3 cut(s) 362, 758, 826
ApoI RAATTY 3 cut(s) 17, 476, 707
AseI ATTAAT 1 cut(s) 429
Asp718I GGTACC 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 929
AspS9I GGNCC 3 cut(s) 62, 434, 975
AsuHPI GGTGA 1 cut(s) 245
AvaII GGWCC 3 cut(s) 62, 434, 975
AvrII CCTAGG 1 cut(s) 929
BaeGI GKGCMC 2 cut(s) 134, 742
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 2 cut(s) 213, 1084
BarI GAAGNNNNNNTAC 2 cut(s) 499, 531
BauI CACGAG 2 cut(s) 683, 831
BbsI GAAGAC 1 cut(s) 514
Bbv12I GWGCWC 4 cut(s) 213, 349, 742, 1084
BbvI GCAGC 3 cut(s) 349, 770, 838
BccI CCATC 1 cut(s) 1111
BciT130I CCWGG 1 cut(s) 652
BciVI GTATCC 1 cut(s) 927
BclI TGATCA 1 cut(s) 547
BfaI CTAG 2 cut(s) 189, 930
BfmI CTRYAG 1 cut(s) 661
BfuAI ACCTGC 1 cut(s) 986
BfuI GTATCC 1 cut(s) 927
BglII AGATCT 1 cut(s) 679
BisI GCNGC 3 cut(s) 363, 759, 827
BlnI CCTAGG 1 cut(s) 929
BlsI GCNGC 3 cut(s) 364, 760, 828
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 1 cut(s) 652
Bme18I GGWCC 3 cut(s) 62, 434, 975
BmgT120I GGNCC 3 cut(s) 62, 434, 975
BmiI GGNNCC 4 cut(s) 173, 577, 927, 976
BmrFI CCNGG 1 cut(s) 652
BmsI GCATC 6 cut(s) 106, 324, 580, 586, 607, 613
BpiI GAAGAC 1 cut(s) 514
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpuEI CTTGAG 3 cut(s) 227, 504, 1128
BsaAI YACGTR 1 cut(s) 1109
BsaJI CCNNGG 1 cut(s) 929
Bsc4I CCNNNNNNNGG 2 cut(s) 730, 836
Bse118I RCCGGY 2 cut(s) 321, 719
Bse1I ACTGG 2 cut(s) 206, 747
Bse3DI GCAATG 2 cut(s) 363, 770
BseBI CCWGG 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 929
BseGI GGATG 1 cut(s) 1122
BseLI CCNNNNNNNGG 2 cut(s) 730, 836
BseMI GCAATG 2 cut(s) 363, 770
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 2 cut(s) 206, 747
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 2 cut(s) 134, 742
BseXI GCAGC 3 cut(s) 349, 770, 838
BshFI GGCC 1 cut(s) 723
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 4 cut(s) 213, 349, 742, 1084
BsiSI CCGG 2 cut(s) 322, 720
BslFI GGGAC 1 cut(s) 988
BslI CCNNNNNNNGG 2 cut(s) 730, 836
BsmFI GGGAC 1 cut(s) 988
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 1 cut(s) 723
Bsp1286I GDGCHC 5 cut(s) 134, 213, 349, 742, 1084
Bsp143I GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 723
BspCNI CTCAG 1 cut(s) 269
BspLI GGNNCC 4 cut(s) 173, 577, 927, 976
BspMI ACCTGC 1 cut(s) 986
BspPI GGATC 1 cut(s) 911
BspQI GCTCTTC 2 cut(s) 337, 1017
BspT107I GGYRCC 1 cut(s) 575
BsrDI GCAATG 2 cut(s) 363, 770
BsrFI RCCGGY 2 cut(s) 321, 719
BsrI ACTGG 2 cut(s) 206, 747
BssAI RCCGGY 2 cut(s) 321, 719
BssECI CCNNGG 1 cut(s) 929
BssMI GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
BssSI CACGAG 2 cut(s) 683, 831
BssT1I CCWWGG 1 cut(s) 929
Bst2BI CACGAG 2 cut(s) 683, 831
Bst2UI CCWGG 1 cut(s) 652
Bst4CI ACNGT 2 cut(s) 277, 406
Bst6I CTCTTC 2 cut(s) 337, 1017
BstAPI GCANNNNNTGC 2 cut(s) 353, 362
BstBAI YACGTR 1 cut(s) 1109
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 1122
BstKTI GATC 7 cut(s) 69, 223, 550, 558, 682, 891, 906
BstMBI GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
BstMWI GCNNNNNNNGC 5 cut(s) 202, 353, 362, 371, 767
BstNI CCWGG 1 cut(s) 652
BstSCI CCNGG 1 cut(s) 650
BstSFI CTRYAG 1 cut(s) 661
BstSLI GKGCMC 2 cut(s) 134, 742
BstV1I GCAGC 3 cut(s) 349, 770, 838
BstV2I GAAGAC 1 cut(s) 514
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BsuI GTATCC 1 cut(s) 927
BsuRI GGCC 1 cut(s) 723
BtsCI GGATG 1 cut(s) 1122
BtsIMutI CAGTG 2 cut(s) 273, 740
BveI ACCTGC 1 cut(s) 986
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 2 cut(s) 321, 719
Cfr13I GGNCC 3 cut(s) 62, 434, 975
Csp6I GTAC 5 cut(s) 77, 576, 633, 655, 1124
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 5 cut(s) 529, 536, 600, 627, 862
CviQI GTAC 5 cut(s) 77, 576, 633, 655, 1124
DdeI CTNAG 1 cut(s) 256
DpnI GATC 7 cut(s) 68, 222, 549, 557, 681, 890, 905
DpnII GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
EaeI YGGCCR 1 cut(s) 721
Eam1104I CTCTTC 2 cut(s) 337, 1017
EarI CTCTTC 2 cut(s) 337, 1017
Ecl136II GAGCTC 2 cut(s) 211, 1082
Eco130I CCWWGG 1 cut(s) 929
Eco24I GRGCYC 2 cut(s) 213, 1084
Eco47I GGWCC 3 cut(s) 62, 434, 975
Eco53kI GAGCTC 2 cut(s) 211, 1082
EcoICRI GAGCTC 2 cut(s) 211, 1082
EcoO109I RGGNCCY 1 cut(s) 975
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 650
EcoT14I CCWWGG 1 cut(s) 929
EcoT22I ATGCAT 2 cut(s) 601, 628
EcoT38I GRGCYC 2 cut(s) 213, 1084
ErhI CCWWGG 1 cut(s) 929
FaeI CATG 5 cut(s) 532, 539, 603, 630, 865
FalI AAGNNNNNCTT 2 cut(s) 1025, 1057
FaqI GGGAC 1 cut(s) 988
FatI CATG 5 cut(s) 528, 535, 599, 626, 861
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 363, 759, 827
FokI GGATG 1 cut(s) 1129
FriOI GRGCYC 2 cut(s) 213, 1084
Fsp4HI GCNGC 3 cut(s) 363, 759, 827
FspBI CTAG 2 cut(s) 189, 930
GluI GCNGC 3 cut(s) 363, 759, 827
HaeIII GGCC 1 cut(s) 723
HapII CCGG 2 cut(s) 322, 720
Hin1II CATG 5 cut(s) 532, 539, 603, 630, 865
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 3 cut(s) 254, 667, 858
HpaII CCGG 2 cut(s) 322, 720
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 740
Hpy188I TCNGA 4 cut(s) 66, 335, 572, 900
Hpy188III TCNNGA 3 cut(s) 218, 437, 685
Hpy8I GTNNAC 1 cut(s) 740
HpyAV CCTTC 5 cut(s) 191, 400, 415, 535, 1107
HpyCH4III ACNGT 2 cut(s) 277, 406
HpyCH4IV ACGT 2 cut(s) 818, 1108
HpyCH4V TGCA 9 cut(s) 356, 593, 599, 620, 626, 740, 758, 775, 805
HpyF10VI GCNNNNNNNGC 5 cut(s) 202, 353, 362, 371, 767
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 2 cut(s) 818, 1108
Hsp92II CATG 5 cut(s) 532, 539, 603, 630, 865
KpnI GGTACC 1 cut(s) 579
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
LguI GCTCTTC 2 cut(s) 337, 1017
LmnI GCTCC 3 cut(s) 537, 1051, 1079
Lsp1109I GCAGC 3 cut(s) 349, 770, 838
LweI GCATC 6 cut(s) 106, 324, 580, 586, 607, 613
MaeI CTAG 2 cut(s) 189, 930
MaeII ACGT 2 cut(s) 818, 1108
MaeIII GTNAC 4 cut(s) 233, 715, 914, 1138
MalI GATC 7 cut(s) 68, 222, 549, 557, 681, 890, 905
MboI GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
MboII GAAGA 6 cut(s) 125, 354, 472, 519, 794, 1034
MfeI CAATTG 2 cut(s) 551, 671
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 5 cut(s) 134, 213, 349, 742, 1084
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 3 cut(s) 259, 285, 774
Mph1103I ATGCAT 2 cut(s) 601, 628
MseI TTAA 2 cut(s) 227, 429
MspI CCGG 2 cut(s) 322, 720
MspR9I CCNGG 1 cut(s) 652
MunI CAATTG 2 cut(s) 551, 671
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 1 cut(s) 652
MwoI GCNNNNNNNGC 5 cut(s) 202, 353, 362, 371, 767
NdeI CATATG 1 cut(s) 376
NdeII GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
NlaIII CATG 5 cut(s) 532, 539, 603, 630, 865
NlaIV GGNNCC 4 cut(s) 173, 577, 927, 976
NmuCI GTSAC 2 cut(s) 233, 715
NsiI ATGCAT 2 cut(s) 601, 628
PciSI GCTCTTC 2 cut(s) 337, 1017
PctI GAATGC 1 cut(s) 566
PfeI GAWTC 2 cut(s) 667, 858
PflMI CCANNNNNTGG 2 cut(s) 730, 836
PkrI GCNGC 3 cut(s) 364, 760, 828
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
Ppu21I YACGTR 1 cut(s) 1109
PpuMI RGGWCCY 1 cut(s) 975
PshBI ATTAAT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 492
Psp124BI GAGCTC 2 cut(s) 213, 1084
Psp5II RGGWCCY 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 650
PspGI CCWGG 1 cut(s) 650
PspN4I GGNNCC 4 cut(s) 173, 577, 927, 976
PspPI GGNCC 3 cut(s) 62, 434, 975
PspPPI RGGWCCY 1 cut(s) 975
PsuI RGATCY 1 cut(s) 679
RsaI GTAC 5 cut(s) 78, 577, 634, 656, 1125
RsaNI GTAC 5 cut(s) 77, 576, 633, 655, 1124
SacI GAGCTC 2 cut(s) 213, 1084
SapI GCTCTTC 2 cut(s) 337, 1017
SaqAI TTAA 2 cut(s) 227, 429
SatI GCNGC 3 cut(s) 363, 759, 827
Sau3AI GATC 7 cut(s) 66, 220, 547, 555, 679, 888, 903
Sau96I GGNCC 3 cut(s) 62, 434, 975
ScaI AGTACT 1 cut(s) 634
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 652
SduI GDGCHC 5 cut(s) 134, 213, 349, 742, 1084
SfaNI GCATC 6 cut(s) 106, 324, 580, 586, 607, 613
SfcI CTRYAG 1 cut(s) 661
SinI GGWCC 3 cut(s) 62, 434, 975
SmlI CTYRAG 3 cut(s) 206, 519, 1143
SmoI CTYRAG 3 cut(s) 206, 519, 1143
SsiI CCGC 1 cut(s) 104
SspMI CTAG 2 cut(s) 189, 930
SstI GAGCTC 2 cut(s) 213, 1084
StyD4I CCNGG 1 cut(s) 650
StyI CCWWGG 1 cut(s) 929
TaaI ACNGT 2 cut(s) 277, 406
TaiI ACGT 2 cut(s) 821, 1111
TaqI TCGA 2 cut(s) 15, 642
TatI WGTACW 2 cut(s) 632, 1123
TfiI GAWTC 2 cut(s) 667, 858
Tru1I TTAA 2 cut(s) 227, 429
Tru9I TTAA 2 cut(s) 227, 429
TscAI CASTG 2 cut(s) 280, 747
TseFI GTSAC 2 cut(s) 233, 715
TseI GCWGC 3 cut(s) 362, 758, 826
Tsp45I GTSAC 2 cut(s) 233, 715
TspDTI ATGAA 8 cut(s) 256, 382, 479, 517, 700, 850, 890, 1085
TspRI CASTG 2 cut(s) 280, 747
Van91I CCANNNNNTGG 2 cut(s) 730, 836
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 3 cut(s) 62, 434, 975
VspI ATTAAT 1 cut(s) 429
XapI RAATTY 3 cut(s) 17, 476, 707
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XmaJI CCTAGG 1 cut(s) 929
XspI CTAG 2 cut(s) 189, 930
ZrmI AGTACT 1 cut(s) 634
Zsp2I ATGCAT 2 cut(s) 601, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.