MD15G1315300.v1.1

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004574
Physical Location & Seq
Forward (+)
32152582 .. 32157628
5047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1315300.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCTTCCGCCGTCCCTGAAAACCTCAGCAGAGAACAGTACGTGTACCTGGCGAAGCTCGCCGAGCAGGCGGAGCGCTACGAGGAGATGGTGAGCTTCATGGAGAAGCTTGTCGTTGGCTCCACCGCCGCCGGGACCGAGCTGACGGTGGAGGAGCGCAACCTGCTCAGCGTCGCCTACAAGAACGTCATCGGCTCGCTCCGCGCCGCCTGGCGCATAATCTCGTCGATCGAGCAGAAGGAGGAAGGCCGCCGCAACGAGGAGCACGTGGCGCTCGTCAAACAGTACAGATCCAAGGTGGAGACCGAGCTCTCCGCCATCTGCGCCGGAATCCTGGAGCTCCTGCAGTCGCACCTTGTTCCTTCGGCTACCACCGGAGAGTCCAAGGTCTTCTACTTGAAGATGAAGGGCGATTACCATCGGTACCTCGCCGAGTTCAAAAATGGCGACGAGAGAAAGACCGCCGCCGAGGACACCATGGTTGCCTACAAGGCTGCTCAGGATATTGCGCTGGCTGATTTGGCGCCTACGCATCCAATAAGGTTGGGGCTGGCGCTCAACTTCTCGGTGTTTTACTACGAGATCCTTAACTCCTCGGAGAAAGCTTGTAGCATGGCTAAACAGGCTTTTGAGGAAGCTATTGCTGAACTTGATACACTGGGAGAGGAATCGTACAAGGATAGCACTTTGATTATGCAACTGCTGAGGGACAACCTCACTCTTTGGACTTCAGATTTGCAGCAGGAGCAGATGGACGAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

255

Amino Acids

28.58

Weight (kDa)

4.85

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 243 1.3e-101 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 171
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 423, 523
AccII CGCG 1 cut(s) 204
AclWI GGATC 2 cut(s) 285, 577
AcuI CTGAAG 1 cut(s) 714
AcvI CACGTG 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 5 cut(s) 41, 47, 287, 425, 674
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 132, 259
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 2 cut(s) 400, 439
AjnI CCWGG 3 cut(s) 48, 209, 333
AluBI AGCT 8 cut(s) 58, 96, 109, 142, 310, 340, 605, 638
AluI AGCT 8 cut(s) 58, 96, 109, 142, 310, 340, 605, 638
Alw21I GWGCWC 3 cut(s) 267, 312, 342
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 2 cut(s) 285, 577
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 494, 739
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
AspS9I GGNCC 1 cut(s) 135
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 135
BanI GGYRCC 2 cut(s) 423, 523
BanII GRGCYC 2 cut(s) 312, 342
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbrPI CACGTG 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 382
Bbv12I GWGCWC 3 cut(s) 267, 312, 342
BbvCI CCTCAGC 2 cut(s) 26, 704
BbvI GCAGC 2 cut(s) 481, 751
BccI CCATC 4 cut(s) 82, 326, 426, 745
BciT130I CCWGG 3 cut(s) 50, 211, 335
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 4 cut(s) 79, 275, 527, 557
BfuAI ACCTGC 1 cut(s) 171
BglI GCCNNNNNGGC 2 cut(s) 68, 491
BisI GCNGC 7 cut(s) 129, 207, 250, 253, 465, 495, 740
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 7 cut(s) 130, 208, 251, 254, 466, 496, 741
Bme1390I CCNGG 4 cut(s) 50, 133, 211, 335
Bme18I GGWCC 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 4 cut(s) 121, 136, 425, 525
BmrFI CCNGG 4 cut(s) 50, 133, 211, 335
BmrI ACTGGG 1 cut(s) 668
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 382
BpmI CTGGAG 1 cut(s) 356
Bpu10I CCTNAGC 3 cut(s) 26, 498, 704
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 133
BsaAI YACGTR 2 cut(s) 43, 268
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 259
Bse1I ACTGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 3 cut(s) 50, 211, 335
BseDI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 132, 259
BseMII CTCAG 4 cut(s) 40, 181, 512, 695
BseNI ACTGG 1 cut(s) 663
BseRI GAGGAG 4 cut(s) 98, 167, 275, 583
BseXI GCAGC 2 cut(s) 481, 751
Bsh1236I CGCG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 2 cut(s) 423, 523
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 3 cut(s) 267, 312, 342
BsiSI CCGG 3 cut(s) 132, 327, 375
BslFI GGGAC 2 cut(s) 148, 722
BslI CCNNNNNNNGG 2 cut(s) 132, 259
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 2 cut(s) 148, 722
BsnI GGCC 1 cut(s) 249
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 3 cut(s) 267, 312, 342
Bsp143I GATC 3 cut(s) 228, 290, 582
Bsp1720I GCTNAGC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 477
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 4 cut(s) 39, 180, 511, 696
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 4 cut(s) 121, 136, 425, 525
BspMAI CTGCAG 1 cut(s) 348
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 285, 577
BspT107I GGYRCC 2 cut(s) 423, 523
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 663
BssECI CCNNGG 5 cut(s) 294, 384, 468, 477, 594
BssMI GATC 3 cut(s) 228, 290, 582
BssNI GRCGYC 1 cut(s) 524
BssT1I CCWWGG 3 cut(s) 294, 384, 477
Bst2UI CCWGG 3 cut(s) 50, 211, 335
Bst4CI ACNGT 3 cut(s) 39, 148, 285
BstACI GRCGYC 1 cut(s) 524
BstBAI YACGTR 2 cut(s) 43, 268
BstC8I GCNNGC 5 cut(s) 60, 69, 197, 513, 552
BstDEI CTNAG 4 cut(s) 26, 167, 498, 704
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 204
BstH2I RGCGCY 4 cut(s) 79, 275, 527, 557
BstHHI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
BstKTI GATC 3 cut(s) 231, 293, 585
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 228, 290, 582
BstMCI CGRYCG 1 cut(s) 231
BstNI CCWGG 3 cut(s) 50, 211, 335
BstSCI CCNGG 4 cut(s) 48, 131, 209, 333
BstSFI CTRYAG 1 cut(s) 344
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 481, 751
BstV2I GAAGAC 1 cut(s) 382
BstX2I RGATCY 2 cut(s) 290, 582
BstYI RGATCY 2 cut(s) 290, 582
BsuRI GGCC 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 1 cut(s) 656
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 5 cut(s) 60, 69, 197, 513, 552
CfoI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
Cfr13I GGNCC 1 cut(s) 135
CseI GACGC 1 cut(s) 160
Csp6I GTAC 5 cut(s) 40, 46, 286, 424, 673
CviAII CATG 3 cut(s) 100, 478, 613
CviQI GTAC 5 cut(s) 40, 46, 286, 424, 673
DdeI CTNAG 4 cut(s) 26, 167, 498, 704
DinI GGCGCC 1 cut(s) 525
DpnI GATC 3 cut(s) 230, 292, 584
DpnII GATC 3 cut(s) 228, 290, 582
EciI GGCGGA 2 cut(s) 86, 304
Ecl136II GAGCTC 2 cut(s) 310, 340
Eco130I CCWWGG 3 cut(s) 294, 384, 477
Eco24I GRGCYC 2 cut(s) 312, 342
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 135
Eco47III AGCGCT 1 cut(s) 77
Eco53kI GAGCTC 2 cut(s) 310, 340
Eco57I CTGAAG 1 cut(s) 714
Eco72I CACGTG 1 cut(s) 268
EcoICRI GAGCTC 2 cut(s) 310, 340
EcoRII CCWGG 3 cut(s) 48, 209, 333
EcoT14I CCWWGG 3 cut(s) 294, 384, 477
EcoT38I GRGCYC 2 cut(s) 312, 342
EgeI GGCGCC 1 cut(s) 525
EheI GGCGCC 1 cut(s) 525
ErhI CCWWGG 3 cut(s) 294, 384, 477
FaeI CATG 3 cut(s) 103, 481, 616
FaiI YATR 5 cut(s) 101, 218, 479, 614, 695
FaqI GGGAC 2 cut(s) 148, 722
FatI CATG 3 cut(s) 99, 477, 612
Fnu4HI GCNGC 7 cut(s) 129, 207, 250, 253, 465, 495, 740
FokI GGATG 1 cut(s) 519
FriOI GRGCYC 2 cut(s) 312, 342
Fsp4HI GCNGC 7 cut(s) 129, 207, 250, 253, 465, 495, 740
GlaI GCGC 9 cut(s) 77, 158, 205, 215, 273, 325, 510, 525, 555
GluI GCNGC 7 cut(s) 129, 207, 250, 253, 465, 495, 740
GsuI CTGGAG 1 cut(s) 356
HaeII RGCGCY 4 cut(s) 79, 275, 527, 557
HaeIII GGCC 1 cut(s) 249
HapII CCGG 3 cut(s) 132, 327, 375
HgaI GACGC 1 cut(s) 160
HhaI GCGC 9 cut(s) 78, 159, 206, 216, 274, 326, 511, 526, 556
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 3 cut(s) 103, 481, 616
Hin6I GCGC 9 cut(s) 76, 157, 204, 214, 272, 324, 509, 524, 554
HinP1I GCGC 9 cut(s) 76, 157, 204, 214, 272, 324, 509, 524, 554
HindIII AAGCTT 2 cut(s) 107, 603
HinfI GANTC 3 cut(s) 330, 380, 668
HpaII CCGG 3 cut(s) 132, 327, 375
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 598, 733
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 3 cut(s) 176, 229, 452
HpyAV CCTTC 4 cut(s) 232, 239, 372, 400
HpyCH4III ACNGT 3 cut(s) 39, 148, 285
HpyCH4IV ACGT 3 cut(s) 42, 186, 267
HpyCH4V TGCA 3 cut(s) 346, 697, 739
HpyF3I CTNAG 4 cut(s) 26, 167, 498, 704
HpySE526I ACGT 3 cut(s) 42, 186, 267
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 3 cut(s) 103, 481, 616
HspAI GCGC 9 cut(s) 76, 157, 204, 214, 272, 324, 509, 524, 554
KasI GGCGCC 1 cut(s) 523
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 228, 290, 582
LmnI GCTCC 8 cut(s) 73, 125, 154, 204, 262, 337, 345, 745
Lsp1109I GCAGC 2 cut(s) 481, 751
LweI GCATC 1 cut(s) 541
MaeII ACGT 3 cut(s) 42, 186, 267
MalI GATC 3 cut(s) 230, 292, 584
MboI GATC 3 cut(s) 228, 290, 582
MboII GAAGA 2 cut(s) 382, 412
MflI RGATCY 2 cut(s) 290, 582
MhlI GDGCHC 3 cut(s) 267, 312, 342
Mly113I GGCGCC 1 cut(s) 524
MlyI GAGTC 1 cut(s) 389
MseI TTAA 2 cut(s) 588, 763
MspI CCGG 3 cut(s) 132, 327, 375
MspR9I CCNGG 4 cut(s) 50, 133, 211, 335
MvaI CCWGG 3 cut(s) 50, 211, 335
MvnI CGCG 1 cut(s) 204
NarI GGCGCC 1 cut(s) 524
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 477
NdeII GATC 3 cut(s) 228, 290, 582
NlaIII CATG 3 cut(s) 103, 481, 616
NlaIV GGNNCC 4 cut(s) 121, 136, 425, 525
NmeAIII GCCGAG 3 cut(s) 88, 457, 493
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 273
PfeI GAWTC 2 cut(s) 330, 668
PfoI TCCNGGA 1 cut(s) 333
PkrI GCNGC 7 cut(s) 130, 208, 251, 254, 466, 496, 741
Ple19I CGATCG 1 cut(s) 231
PleI GAGTC 1 cut(s) 388
PluTI GGCGCC 1 cut(s) 527
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 2 cut(s) 43, 268
Psp124BI GAGCTC 2 cut(s) 312, 342
Psp6I CCWGG 3 cut(s) 48, 209, 333
PspCI CACGTG 1 cut(s) 268
PspGI CCWGG 3 cut(s) 48, 209, 333
PspN4I GGNNCC 4 cut(s) 121, 136, 425, 525
PspPI GGNCC 1 cut(s) 135
PstI CTGCAG 1 cut(s) 348
PsuI RGATCY 2 cut(s) 290, 582
PvuI CGATCG 1 cut(s) 231
RsaI GTAC 5 cut(s) 41, 47, 287, 425, 674
RsaNI GTAC 5 cut(s) 40, 46, 286, 424, 673
SacI GAGCTC 2 cut(s) 312, 342
SaqAI TTAA 2 cut(s) 588, 763
SatI GCNGC 7 cut(s) 129, 207, 250, 253, 465, 495, 740
Sau3AI GATC 3 cut(s) 228, 290, 582
Sau96I GGNCC 1 cut(s) 135
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 4 cut(s) 50, 133, 211, 335
SduI GDGCHC 3 cut(s) 267, 312, 342
SfaNI GCATC 1 cut(s) 541
SfcI CTRYAG 1 cut(s) 344
SfoI GGCGCC 1 cut(s) 525
SinI GGWCC 1 cut(s) 135
SspDI GGCGCC 1 cut(s) 523
SstI GAGCTC 2 cut(s) 312, 342
StyD4I CCNGG 4 cut(s) 48, 131, 209, 333
StyI CCWWGG 3 cut(s) 294, 384, 477
TaaI ACNGT 3 cut(s) 39, 148, 285
TaiI ACGT 3 cut(s) 45, 189, 270
TaqI TCGA 2 cut(s) 227, 231
TaqII GACCGA 2 cut(s) 152, 320
TatI WGTACW 1 cut(s) 285
TauI GCSGC 5 cut(s) 131, 209, 252, 255, 467
TfiI GAWTC 2 cut(s) 330, 668
Tru1I TTAA 2 cut(s) 588, 763
Tru9I TTAA 2 cut(s) 588, 763
TscAI CASTG 1 cut(s) 663
TseI GCWGC 2 cut(s) 494, 739
TspDTI ATGAA 2 cut(s) 88, 419
TspRI CASTG 1 cut(s) 663
VpaK11BI GGWCC 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.