Rh2CG232100

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004574
Physical Location & Seq
Forward (+)
23580548 .. 23586193
5646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG232100.1

Sequence Viewer

Length: 693 bp
ATGGCGTCCGCCGTGCCGGAAACCCTAAGCCGCGAGCAGTACGTGTACATGGCGAAGCTCGCCGAGCAAGCCGAGCGCTACGAGGAGATGGTCAGCTTCATGGAGAAGCTCGTCGTTGCCTCGACCGCCGCCGGCACCGAGCTCACCGTCGAGGAGCGCAACCTCCTCAGCGTCGCCTACAAGAACGTCATCGGCTCCCTCCGCGCCGCCTGGCGCATCGTCTCCTCCATCGAGCAGAAGGAGGAGGGCCGCCGCAACGAGGAGCACGTGGCGCTCGTCAAGCAGTACAGATCTAAAGTCGAGACTGAGCTCTCGGCCATCTGCGCCGGAATTCTCGACCTCCTTCAGTCGCACCTTGTGCCGTCGGCCACCGCCGGCGAGTCCAAGGTGTTTTACTTGAAGATGAAGGGGGATTACCATCGGTACCTTGCTGAGTTTAAGAACGGCGATGAGAGGAAGACTGCTGCTGAGGATACTATGCTGGCTTACAAGGCTGCTCAGGATATTGCGTTGGCTGATTTGGCTCCTACACATCCTATAAGGCTGGGGCTGGCGCTTAACTTTTCGGTGTTTTACTATGAGATCCTCAATTCCTCAGAGAAGGCTTGCAGCATGGCGAAGCAGGTTTGGGCTCATAAAATTCTTTTAATTTATTATAATTCTGTTTTACTCTGCATGTCTCTGATCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

230

Amino Acids

25.85

Weight (kDa)

6.01

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 213 3.3e-81 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
Acc36I ACCTGC 1 cut(s) 613
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 134, 423
AccII CGCG 2 cut(s) 33, 204
AciI CCGC 9 cut(s) 9, 31, 126, 129, 202, 207, 250, 253, 372
AclWI GGATC 1 cut(s) 577
AcoI YGGCCR 2 cut(s) 315, 366
AcsI RAATTY 2 cut(s) 330, 639
AcuI CTGAAG 1 cut(s) 329
AcvI CACGTG 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 358
AfaI GTAC 4 cut(s) 41, 47, 287, 425
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 5 cut(s) 58, 96, 109, 142, 310
AluI AGCT 5 cut(s) 58, 96, 109, 142, 310
Alw21I GWGCWC 3 cut(s) 144, 267, 312
Alw26I GTCTC 3 cut(s) 226, 296, 684
AlwI GGATC 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 3 cut(s) 247, 315, 366
ApeKI GCWGC 3 cut(s) 464, 494, 609
ApoI RAATTY 2 cut(s) 330, 639
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 7 cut(s) 78, 159, 206, 216, 274, 326, 556
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 136
BanI GGYRCC 2 cut(s) 134, 423
BanII GRGCYC 3 cut(s) 144, 312, 634
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbrPI CACGTG 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 3 cut(s) 144, 267, 312
BbvCI CCTCAGC 2 cut(s) 167, 468
BbvI GCAGC 3 cut(s) 451, 481, 621
BccI CCATC 4 cut(s) 82, 236, 326, 426
BceAI ACGGC 2 cut(s) 346, 460
BciT130I CCWGG 1 cut(s) 211
BciVI GTATCC 1 cut(s) 466
BclI TGATCA 1 cut(s) 684
BcoDI GTCTC 3 cut(s) 226, 296, 684
BfoI RGCGCY 3 cut(s) 79, 275, 557
BfuAI ACCTGC 1 cut(s) 613
BfuI GTATCC 1 cut(s) 466
BglII AGATCT 1 cut(s) 290
BisI GCNGC 8 cut(s) 31, 129, 207, 250, 253, 465, 495, 610
BlsI GCNGC 8 cut(s) 32, 130, 208, 251, 254, 466, 496, 611
Bme1390I CCNGG 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 4 cut(s) 136, 196, 425, 525
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 464
Bpu10I CCTNAGC 4 cut(s) 26, 167, 468, 498
BsaAI YACGTR 2 cut(s) 43, 268
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 384
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 2 cut(s) 131, 374
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 211
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 6 cut(s) 181, 297, 423, 459, 512, 609
BseRI GAGGAG 6 cut(s) 98, 155, 167, 214, 257, 275
BseXI GCAGC 3 cut(s) 451, 481, 621
BseYI CCCAGC 1 cut(s) 544
Bsh1236I CGCG 2 cut(s) 33, 204
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 249, 317, 368
BshNI GGYRCC 2 cut(s) 134, 423
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 3 cut(s) 144, 267, 312
BsiSI CCGG 4 cut(s) 17, 132, 327, 375
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 3 cut(s) 226, 296, 684
BsmBI CGTCTC 1 cut(s) 226
BsnI GGCC 3 cut(s) 249, 317, 368
Bsp1286I GDGCHC 4 cut(s) 144, 267, 312, 634
Bsp1407I TGTACA 1 cut(s) 45
Bsp143I GATC 3 cut(s) 290, 582, 684
BspACI CCGC 9 cut(s) 9, 31, 126, 129, 202, 207, 250, 253, 372
BspANI GGCC 3 cut(s) 249, 317, 368
BspCNI CTCAG 6 cut(s) 180, 298, 424, 460, 511, 608
BspFNI CGCG 2 cut(s) 33, 204
BspLI GGNNCC 4 cut(s) 136, 196, 425, 525
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 1 cut(s) 577
BspT107I GGYRCC 2 cut(s) 134, 423
BsrFI RCCGGY 2 cut(s) 131, 374
BsrGI TGTACA 1 cut(s) 45
BssAI RCCGGY 2 cut(s) 131, 374
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 3 cut(s) 290, 582, 684
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 211
Bst4CI ACNGT 1 cut(s) 148
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 358
BstAUI TGTACA 1 cut(s) 45
BstBAI YACGTR 2 cut(s) 43, 268
BstC8I GCNNGC 8 cut(s) 35, 60, 69, 133, 376, 483, 552, 607
BstDEI CTNAG 7 cut(s) 26, 167, 306, 432, 468, 498, 595
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 2 cut(s) 33, 204
BstH2I RGCGCY 3 cut(s) 79, 275, 557
BstHHI GCGC 7 cut(s) 78, 159, 206, 216, 274, 326, 556
BstKTI GATC 3 cut(s) 293, 585, 687
BstMAI GTCTC 3 cut(s) 226, 296, 684
BstMBI GATC 3 cut(s) 290, 582, 684
BstMCI CGRYCG 1 cut(s) 126
BstNI CCWGG 1 cut(s) 211
BstNSI RCATGY 1 cut(s) 679
BstSCI CCNGG 1 cut(s) 209
BstUI CGCG 2 cut(s) 33, 204
BstV1I GCAGC 3 cut(s) 451, 481, 621
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 2 cut(s) 290, 582
BstYI RGATCY 2 cut(s) 290, 582
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 3 cut(s) 249, 317, 368
BtgZI GCGATG 1 cut(s) 462
BtsCI GGATG 1 cut(s) 532
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 8 cut(s) 35, 60, 69, 133, 376, 483, 552, 607
CfoI GCGC 7 cut(s) 78, 159, 206, 216, 274, 326, 556
Cfr10I RCCGGY 2 cut(s) 131, 374
Cfr13I GGNCC 1 cut(s) 247
CseI GACGC 1 cut(s) 160
Csp6I GTAC 4 cut(s) 40, 46, 286, 424
CviAII CATG 5 cut(s) 49, 100, 613, 676, 690
CviQI GTAC 4 cut(s) 40, 46, 286, 424
DdeI CTNAG 7 cut(s) 26, 167, 306, 432, 468, 498, 595
DpnI GATC 3 cut(s) 292, 584, 686
DpnII GATC 3 cut(s) 290, 582, 684
DraIII CACNNNGTG 1 cut(s) 358
EaeI YGGCCR 2 cut(s) 315, 366
Ecl136II GAGCTC 2 cut(s) 142, 310
Eco130I CCWWGG 1 cut(s) 384
Eco24I GRGCYC 3 cut(s) 144, 312, 634
Eco47III AGCGCT 1 cut(s) 77
Eco53kI GAGCTC 2 cut(s) 142, 310
Eco57I CTGAAG 1 cut(s) 329
Eco72I CACGTG 1 cut(s) 268
EcoICRI GAGCTC 2 cut(s) 142, 310
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 3 cut(s) 144, 312, 634
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 5 cut(s) 52, 103, 616, 679, 693
FatI CATG 5 cut(s) 48, 99, 612, 675, 689
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 8 cut(s) 31, 129, 207, 250, 253, 465, 495, 610
FokI GGATG 1 cut(s) 519
FriOI GRGCYC 3 cut(s) 144, 312, 634
Fsp4HI GCNGC 8 cut(s) 31, 129, 207, 250, 253, 465, 495, 610
GlaI GCGC 7 cut(s) 77, 158, 205, 215, 273, 325, 555
GluI GCNGC 8 cut(s) 31, 129, 207, 250, 253, 465, 495, 610
GsaI CCCAGC 1 cut(s) 548
HaeII RGCGCY 3 cut(s) 79, 275, 557
HaeIII GGCC 3 cut(s) 249, 317, 368
HapII CCGG 4 cut(s) 17, 132, 327, 375
HgaI GACGC 1 cut(s) 160
HhaI GCGC 7 cut(s) 78, 159, 206, 216, 274, 326, 556
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 52, 103, 616, 679, 693
Hin6I GCGC 7 cut(s) 76, 157, 204, 214, 272, 324, 554
HinP1I GCGC 7 cut(s) 76, 157, 204, 214, 272, 324, 554
HinfI GANTC 1 cut(s) 380
HpaII CCGG 4 cut(s) 17, 132, 327, 375
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 598, 684
Hpy188III TCNNGA 3 cut(s) 301, 335, 500
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 4 cut(s) 116, 152, 176, 367
HpyAV CCTTC 4 cut(s) 232, 353, 400, 595
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 3 cut(s) 42, 186, 267
HpyCH4V TGCA 2 cut(s) 609, 675
HpyF3I CTNAG 7 cut(s) 26, 167, 306, 432, 468, 498, 595
HpySE526I ACGT 3 cut(s) 42, 186, 267
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 52, 103, 616, 679, 693
HspAI GCGC 7 cut(s) 76, 157, 204, 214, 272, 324, 554
KpnI GGTACC 1 cut(s) 427
KroI GCCGGC 2 cut(s) 131, 374
KroNI GCCGGC 2 cut(s) 133, 376
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 3 cut(s) 290, 582, 684
LmnI GCTCC 4 cut(s) 154, 200, 262, 529
Lsp1109I GCAGC 3 cut(s) 451, 481, 621
LweI GCATC 1 cut(s) 225
MaeII ACGT 3 cut(s) 42, 186, 267
MalI GATC 3 cut(s) 292, 584, 686
MboI GATC 3 cut(s) 290, 582, 684
MboII GAAGA 2 cut(s) 412, 469
MflI RGATCY 2 cut(s) 290, 582
MhlI GDGCHC 4 cut(s) 144, 267, 312, 634
MluCI AATT 5 cut(s) 330, 589, 639, 648, 658
MlyI GAGTC 1 cut(s) 389
MreI CGCCGGCG 1 cut(s) 374
MroNI GCCGGC 2 cut(s) 131, 374
MseI TTAA 3 cut(s) 438, 558, 647
MspI CCGG 4 cut(s) 17, 132, 327, 375
MspR9I CCNGG 1 cut(s) 211
MvaI CCWGG 1 cut(s) 211
MvnI CGCG 2 cut(s) 33, 204
NaeI GCCGGC 2 cut(s) 133, 376
NdeII GATC 3 cut(s) 290, 582, 684
NgoMIV GCCGGC 2 cut(s) 131, 374
NlaIII CATG 5 cut(s) 52, 103, 616, 679, 693
NlaIV GGNNCC 4 cut(s) 136, 196, 425, 525
NmeAIII GCCGAG 3 cut(s) 88, 97, 293
NspI RCATGY 1 cut(s) 679
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 273
PdiI GCCGGC 2 cut(s) 133, 376
PkrI GCNGC 8 cut(s) 32, 130, 208, 251, 254, 466, 496, 611
PleI GAGTC 1 cut(s) 388
PmaCI CACGTG 1 cut(s) 268
PmlI CACGTG 1 cut(s) 268
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 2 cut(s) 43, 268
PsiI TTATAA 1 cut(s) 657
Psp124BI GAGCTC 2 cut(s) 144, 312
Psp6I CCWGG 1 cut(s) 209
PspCI CACGTG 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 544
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 4 cut(s) 136, 196, 425, 525
PspPI GGNCC 1 cut(s) 247
PsuI RGATCY 2 cut(s) 290, 582
RsaI GTAC 4 cut(s) 41, 47, 287, 425
RsaNI GTAC 4 cut(s) 40, 46, 286, 424
SacI GAGCTC 2 cut(s) 144, 312
SaqAI TTAA 3 cut(s) 438, 558, 647
SatI GCNGC 8 cut(s) 31, 129, 207, 250, 253, 465, 495, 610
Sau3AI GATC 3 cut(s) 290, 582, 684
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 4 cut(s) 144, 267, 312, 634
SfaNI GCATC 1 cut(s) 225
SgrAI CRCCGGYG 1 cut(s) 374
Sse9I AATT 5 cut(s) 330, 589, 639, 648, 658
SsiI CCGC 9 cut(s) 9, 31, 126, 129, 202, 207, 250, 253, 372
SstI GAGCTC 2 cut(s) 144, 312
StyD4I CCNGG 1 cut(s) 209
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 3 cut(s) 45, 189, 270
TaqI TCGA 5 cut(s) 122, 150, 231, 300, 336
TasI AATT 5 cut(s) 330, 589, 639, 648, 658
TatI WGTACW 2 cut(s) 45, 285
TauI GCSGC 5 cut(s) 33, 131, 209, 252, 255
Tru1I TTAA 3 cut(s) 438, 558, 647
Tru9I TTAA 3 cut(s) 438, 558, 647
TseI GCWGC 3 cut(s) 464, 494, 609
TspDTI ATGAA 2 cut(s) 88, 419
XapI RAATTY 2 cut(s) 330, 639
XceI RCATGY 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.