FvH4_1g19920

14-3-3-like protein GF14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004574
Physical Location & Seq
Forward (+)
12054375 .. 12058501
4127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19920.t1

Sequence Viewer

Length: 762 bp
ATGGCCTCCGCCGTGCCCGAAACCCTAAGCCGCGAGCAGTACGTCTACATGGCCAAGCTCGCCGAGCAAGCCGAGCGCTACGAGGAGATGGTCAGCTTCATGGAGAAGCTCGTCGTCGCCTCCACCGCCGCCGGCAGCGAGCTGACGGTGGAGGAGCGCAACCTCCTCAGCGTCGCCTACAAGAACGTCATCGGCTCCCTACGCGCCGCCTGGCGCATCGTCTCCTCCATCGAGCAGAAGGAGGAGGGCCGCCGCAACGAGGACCACGTCGCGCTCGTCAAGCAGTACAGATCTAAAGTCGAGACCGAGCTAACGGCGATATGCGCCGGAATTCTGGACCTCCTGCAGTCGCACCTGGTGCCGTCGGCCACCGCCGGCGAGTCGAAGGTGTTTTACTTGAAGATGAAGGGGGATTACCATCGGTACCTTGCTGAGTTTAAGAACGGCGATGAGAGGAAGACTGCTGCTGAGGACACTATGCTGGCTTACAAGGCCGCGCAGGATATTGCTCTGGCTGATTTGGCTCCAACACATCCTATAAGGCTGGGGCTGGCGCTTAATTTTTCGGTGTTTTACTATGAGATCCTCAATTCCTCTGAGAAGGCTTGCAGCATGGCTAAGCAGGCCTTTGAAGAAGCAATTGCTGAACTGGATACGCTGGGAGAGGAATCATACAAGGACAGCACTTTAATCATGCAACTTTTGAGGGACAACCTCACTCTTTGGACTTCTGATTTGCAGGAGCAAATGGATGAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

254

Amino Acids

28.41

Weight (kDa)

4.83

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 17 - 243 2.5e-101 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 358, 423
AccI GTMKAC 1 cut(s) 45
AccII CGCG 4 cut(s) 33, 204, 272, 497
AciI CCGC 9 cut(s) 9, 31, 126, 129, 207, 250, 253, 372, 495
AclWI GGATC 1 cut(s) 577
AcoI YGGCCR 2 cut(s) 51, 366
AcsI RAATTY 1 cut(s) 330
AdeI CACNNNGTG 1 cut(s) 358
AfaI GTAC 3 cut(s) 41, 287, 425
AfeI AGCGCT 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 2 cut(s) 400, 632
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 2 cut(s) 209, 354
AluBI AGCT 5 cut(s) 58, 96, 109, 142, 310
AluI AGCT 5 cut(s) 58, 96, 109, 142, 310
Alw26I GTCTC 2 cut(s) 226, 296
AlwI GGATC 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 77
AoxI GGCC 6 cut(s) 3, 51, 247, 366, 492, 624
ApeKI GCWGC 3 cut(s) 135, 464, 609
ApoI RAATTY 1 cut(s) 330
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 499, 556
AspS9I GGNCC 3 cut(s) 247, 262, 337
AvaII GGWCC 2 cut(s) 262, 337
BaeGI GKGCMC 1 cut(s) 18
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 2 cut(s) 358, 423
BarI GAAGNNNNNNTAC 4 cut(s) 377, 398, 409, 430
BbsI GAAGAC 1 cut(s) 464
BbvCI CCTCAGC 2 cut(s) 167, 468
BbvI GCAGC 3 cut(s) 147, 451, 621
BccI CCATC 3 cut(s) 82, 236, 426
BceAI ACGGC 3 cut(s) 330, 346, 460
BciT130I CCWGG 2 cut(s) 211, 356
BciVI GTATCC 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 226, 296
BfmI CTRYAG 1 cut(s) 344
BfoI RGCGCY 2 cut(s) 79, 557
BfuI GTATCC 1 cut(s) 646
BglII AGATCT 1 cut(s) 290
BisI GCNGC 9 cut(s) 31, 129, 136, 207, 250, 253, 465, 495, 610
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 9 cut(s) 32, 130, 137, 208, 251, 254, 466, 496, 611
Bme1390I CCNGG 2 cut(s) 211, 356
Bme18I GGWCC 2 cut(s) 262, 337
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 247, 262, 337
BmiI GGNNCC 4 cut(s) 196, 360, 425, 525
BmrFI CCNGG 2 cut(s) 211, 356
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 464
Bpu10I CCTNAGC 3 cut(s) 26, 167, 468
Bpu1102I GCTNAGC 1 cut(s) 618
BsaBI GATNNNNATC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 296
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 2 cut(s) 131, 374
Bse1I ACTGG 1 cut(s) 654
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 2 cut(s) 211, 356
BseGI GGATG 2 cut(s) 532, 757
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 4 cut(s) 181, 423, 459, 588
BseNI ACTGG 1 cut(s) 654
BseRI GAGGAG 5 cut(s) 98, 155, 167, 214, 257
BseSI GKGCMC 1 cut(s) 18
BseXI GCAGC 3 cut(s) 147, 451, 621
BseYI CCCAGC 2 cut(s) 544, 658
Bsh1236I CGCG 4 cut(s) 33, 204, 272, 497
BshFI GGCC 6 cut(s) 5, 53, 249, 368, 494, 626
BshNI GGYRCC 2 cut(s) 358, 423
BsiSI CCGG 3 cut(s) 132, 327, 375
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 226, 296
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 6 cut(s) 5, 53, 249, 368, 494, 626
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 290, 582
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 9 cut(s) 9, 31, 126, 129, 207, 250, 253, 372, 495
BspANI GGCC 6 cut(s) 5, 53, 249, 368, 494, 626
BspCNI CTCAG 4 cut(s) 180, 424, 460, 589
BspFNI CGCG 4 cut(s) 33, 204, 272, 497
BspLI GGNNCC 4 cut(s) 196, 360, 425, 525
BspMAI CTGCAG 1 cut(s) 348
BspPI GGATC 1 cut(s) 577
BspT107I GGYRCC 2 cut(s) 358, 423
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 2 cut(s) 131, 374
BsrI ACTGG 1 cut(s) 654
BssAI RCCGGY 2 cut(s) 131, 374
BssMI GATC 2 cut(s) 290, 582
Bst2UI CCWGG 2 cut(s) 211, 356
Bst4CI ACNGT 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 358
BstDEI CTNAG 6 cut(s) 26, 167, 432, 468, 597, 618
BstF5I GGATG 2 cut(s) 532, 757
BstFNI CGCG 4 cut(s) 33, 204, 272, 497
BstH2I RGCGCY 2 cut(s) 79, 557
BstHHI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 499, 556
BstKTI GATC 2 cut(s) 293, 585
BstMAI GTCTC 2 cut(s) 226, 296
BstMBI GATC 2 cut(s) 290, 582
BstNI CCWGG 2 cut(s) 211, 356
BstSCI CCNGG 2 cut(s) 209, 354
BstSFI CTRYAG 1 cut(s) 344
BstSLI GKGCMC 1 cut(s) 18
BstUI CGCG 4 cut(s) 33, 204, 272, 497
BstV1I GCAGC 3 cut(s) 147, 451, 621
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 2 cut(s) 290, 582
BstYI RGATCY 2 cut(s) 290, 582
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 6 cut(s) 5, 53, 249, 368, 494, 626
BtgZI GCGATG 1 cut(s) 462
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 2 cut(s) 532, 757
CfoI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 499, 556
Cfr10I RCCGGY 2 cut(s) 131, 374
Cfr13I GGNCC 3 cut(s) 247, 262, 337
CseI GACGC 1 cut(s) 160
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 3 cut(s) 40, 286, 424
CviAII CATG 4 cut(s) 49, 100, 613, 694
CviQI GTAC 3 cut(s) 40, 286, 424
DdeI CTNAG 6 cut(s) 26, 167, 432, 468, 597, 618
DpnI GATC 2 cut(s) 292, 584
DpnII GATC 2 cut(s) 290, 582
DraIII CACNNNGTG 1 cut(s) 358
EaeI YGGCCR 2 cut(s) 51, 366
Eco147I AGGCCT 1 cut(s) 626
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 262, 337
Eco47III AGCGCT 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 2 cut(s) 209, 354
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 4 cut(s) 52, 103, 616, 697
FaiI YATR 9 cut(s) 50, 101, 322, 479, 539, 579, 614, 673, 695
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 722
FatI CATG 4 cut(s) 48, 99, 612, 693
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 9 cut(s) 31, 129, 136, 207, 250, 253, 465, 495, 610
FokI GGATG 1 cut(s) 519
Fsp4HI GCNGC 9 cut(s) 31, 129, 136, 207, 250, 253, 465, 495, 610
GlaI GCGC 8 cut(s) 77, 158, 205, 215, 273, 325, 498, 555
GluI GCNGC 9 cut(s) 31, 129, 136, 207, 250, 253, 465, 495, 610
GsaI CCCAGC 2 cut(s) 548, 662
HaeII RGCGCY 2 cut(s) 79, 557
HaeIII GGCC 6 cut(s) 5, 53, 249, 368, 494, 626
HapII CCGG 3 cut(s) 132, 327, 375
HgaI GACGC 1 cut(s) 160
HhaI GCGC 8 cut(s) 78, 159, 206, 216, 274, 326, 499, 556
Hin1II CATG 4 cut(s) 52, 103, 616, 697
Hin6I GCGC 8 cut(s) 76, 157, 204, 214, 272, 324, 497, 554
HinP1I GCGC 8 cut(s) 76, 157, 204, 214, 272, 324, 497, 554
HinfI GANTC 2 cut(s) 380, 668
HpaII CCGG 3 cut(s) 132, 327, 375
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 598, 733
Hpy188III TCNNGA 2 cut(s) 301, 335
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 5 cut(s) 116, 119, 176, 272, 367
HpyAV CCTTC 4 cut(s) 232, 379, 400, 595
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 3 cut(s) 42, 186, 267
HpyCH4V TGCA 4 cut(s) 346, 609, 697, 739
HpyF3I CTNAG 6 cut(s) 26, 167, 432, 468, 597, 618
HpySE526I ACGT 3 cut(s) 42, 186, 267
Hsp92II CATG 4 cut(s) 52, 103, 616, 697
HspAI GCGC 8 cut(s) 76, 157, 204, 214, 272, 324, 497, 554
KpnI GGTACC 1 cut(s) 427
KroI GCCGGC 2 cut(s) 131, 374
KroNI GCCGGC 2 cut(s) 133, 376
Kzo9I GATC 2 cut(s) 290, 582
LmnI GCTCC 4 cut(s) 154, 200, 529, 742
Lsp1109I GCAGC 3 cut(s) 147, 451, 621
LweI GCATC 1 cut(s) 225
MabI ACCWGGT 1 cut(s) 354
MaeII ACGT 3 cut(s) 42, 186, 267
MalI GATC 2 cut(s) 292, 584
MboI GATC 2 cut(s) 290, 582
MboII GAAGA 3 cut(s) 412, 469, 644
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 2 cut(s) 290, 582
MhlI GDGCHC 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 4 cut(s) 330, 559, 589, 639
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 551
Mox20I TGGCCA 1 cut(s) 53
MreI CGCCGGCG 1 cut(s) 374
MroNI GCCGGC 2 cut(s) 131, 374
MscI TGGCCA 1 cut(s) 53
MseI TTAA 4 cut(s) 438, 558, 689, 760
Msp20I TGGCCA 1 cut(s) 53
MspI CCGG 3 cut(s) 132, 327, 375
MspR9I CCNGG 2 cut(s) 211, 356
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 2 cut(s) 211, 356
MvnI CGCG 4 cut(s) 33, 204, 272, 497
NaeI GCCGGC 2 cut(s) 133, 376
NdeII GATC 2 cut(s) 290, 582
NgoMIV GCCGGC 2 cut(s) 131, 374
NlaIII CATG 4 cut(s) 52, 103, 616, 697
NlaIV GGNNCC 4 cut(s) 196, 360, 425, 525
NmeAIII GCCGAG 2 cut(s) 88, 97
PceI AGGCCT 1 cut(s) 626
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 273
PdiI GCCGGC 2 cut(s) 133, 376
PfeI GAWTC 1 cut(s) 668
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 9 cut(s) 32, 130, 137, 208, 251, 254, 466, 496, 611
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 2 cut(s) 209, 354
PspFI CCCAGC 2 cut(s) 544, 658
PspGI CCWGG 2 cut(s) 209, 354
PspN4I GGNNCC 4 cut(s) 196, 360, 425, 525
PspPI GGNCC 3 cut(s) 247, 262, 337
PstI CTGCAG 1 cut(s) 348
PsuI RGATCY 2 cut(s) 290, 582
PsyI GACNNNGTC 1 cut(s) 266
RsaI GTAC 3 cut(s) 41, 287, 425
RsaNI GTAC 3 cut(s) 40, 286, 424
SaqAI TTAA 4 cut(s) 438, 558, 689, 760
SatI GCNGC 9 cut(s) 31, 129, 136, 207, 250, 253, 465, 495, 610
Sau3AI GATC 2 cut(s) 290, 582
Sau96I GGNCC 3 cut(s) 247, 262, 337
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 211, 356
SduI GDGCHC 1 cut(s) 18
SexAI ACCWGGT 1 cut(s) 354
SfaNI GCATC 1 cut(s) 225
SfcI CTRYAG 1 cut(s) 344
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 2 cut(s) 262, 337
Sse9I AATT 4 cut(s) 330, 559, 589, 639
SseBI AGGCCT 1 cut(s) 626
SsiI CCGC 9 cut(s) 9, 31, 126, 129, 207, 250, 253, 372, 495
StuI AGGCCT 1 cut(s) 626
StyD4I CCNGG 2 cut(s) 209, 354
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 3 cut(s) 45, 189, 270
TaqI TCGA 3 cut(s) 231, 300, 383
TaqII GACCGA 1 cut(s) 320
TasI AATT 4 cut(s) 330, 559, 589, 639
TatI WGTACW 1 cut(s) 285
TauI GCSGC 6 cut(s) 33, 131, 209, 252, 255, 497
TfiI GAWTC 1 cut(s) 668
Tru1I TTAA 4 cut(s) 438, 558, 689, 760
Tru9I TTAA 4 cut(s) 438, 558, 689, 760
TseI GCWGC 3 cut(s) 135, 464, 609
TspDTI ATGAA 2 cut(s) 88, 419
Tth111I GACNNNGTC 1 cut(s) 266
VpaK11BI GGWCC 2 cut(s) 262, 337
XapI RAATTY 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.